Two-dimensional gel electrophoresis in bacterial proteomics

作     者：Shirly O.T.Curreem Rory M.Watt Susanna K.P.Lau Patrick C.Y.Woo 

作者机构：Department of MicrobiologyThe University of Hong KongQueen Mary HospitalHong KongChina Oral BiosciencesFaculty of DentistryThe University of Hong KongQueen Mary HospitalHong KongChina State Key Laboratory of Emerging Infectious DiseasesDepartment of MicrobiologyThe University of Hong KongQueen Mary HospitalHong KongChina Research Centre of Infection and ImmunologyThe University of Hong KongQueen Mary HospitalHong KongChina Carol Yu Centre of InfectionThe University of Hong KongHong KongChina 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2012年第3卷第5期

页      面：346-363页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：This work was partly supported by the Research Fund for the Control of Infectious Diseases(Commissioned study)of the Health,Welfare and Food Bureau of the Hong Kong SAR Government,Research Grant Council Grant University Development Fund 

主　　题：two-dimensional gel electrophoresis bacte-ria proteomics 

摘      要：Two-dimensional gel electrophoresis(2-DE)is a gel-based technique widely used for analyzing the pro-tein composition of biological *** is capable of resolving complex mixtures containing more than a thousand protein components into individual protein spots through the coupling of two orthogonal bio-physical separation techniques:isoelectric focusing(first dimension)and polyacrylamide gel electrophore-sis(second dimension).2-DE is ideally suited for ana-lyzing the entire expressed protein complement of a bacterial cell:its *** relative simplicity and good reproducibility have led to 2-DE being widely used for exploring proteomics within a wide range of envi-ronmental and medically-relevant *** we give a broad overview of the basic principles and his-torical development of gel-based proteomics,and how this powerful approach can be applied for studying bacterial biology and *** highlight specific 2-DE applications that can be used to analyze when,where and how much proteins are *** links between proteomics,genomics and mass spectrometry are *** explore how proteomics involving tandem mass spectrometry can be used to analyze(post-translational)protein modifications or to identify proteins of unknown origin by de novo peptide *** use of proteome fractionation tech-niques and non-gel-based proteomic approaches are also *** highlight how the analysis of pro-teins secreted by bacterial cells(secretomes or exo-proteomes)can be used to study infection processes or the immune *** review is aimed at non-specialists who wish to gain a concise,compre-hensive and contemporary overview of the nature and applications of bacterial proteomics.