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Chinese Journal of Oceanology and Limnology 1998年第S1期16卷 17-24页

作者： Yoshikazu Kawata shin-ichi yano Hiroyuki Kojima(Osaka Mational Research Institute. Agency of Industrial Science and Technology, Ikeda, Osaka 563, Japan)

An efficient and simple method for constructing a genomic DNA library using a TAcloning vector is presented. It is based on the sonicative cleavage of genomic DNA and modification offragment ends with Taq DNA polymerase, followed by ligation using a TA vector. This method was ap-plied for cloning of the phytoene synthase gene crtB from Spirulina platensis. This method is useful whengenomic DNA cannot be efficiently digested with restriction enzymes, a problem often encountered duringthe construction of a genomic DNA library of cyanobacteria.

关键词： genomic library Spirulina phytoene synthase carotenoid

Fabrication of Mie-resonant silicon nanoparticles using laser annealing for surface-enhanced fluorescence spectroscopy

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Microsystems & Nanoengineering 2024年第2期10卷 133-141页

作者： Tatsuya Fukuta Ryo Kato Takuo Tanaka Taka-aki yano Institute of Post-LED Photonics Tokushima University2-1 Minami-JosanjimaTokushima770-8506Japan Innovative Photon Manipulation Research Team RIKEN Center for Advanced PhotonicsWakoSaitama351-0198Japan Metamaterials Laboratory RIKEN Cluster for Pioneering ResearchWakoSaitama351-0109Japan

Silicon nanostructures with unique Mie resonances have garnered considerable attention in the field of ***,we present a simple and efficient method for the fabrication of silicon(Si)nanoparticle substrates using continuous-wave(CW)laser *** resulting silicon nanoparticles exhibit Mie resonances in the visible region,and their resonant wavelengths can be precisely ***,laser-annealed silicon nanoparticle substrates show a 60-fold enhancement in *** tunable and fluorescence-enhancing silicon nanoparticle platform has tremendous potential for highly sensitive fluorescence sensing and biomedical imaging applications.

关键词： nanoparticles annealing resonant

EXTRACELLULAR RELEASE OF β-LACTAMASE BY OSMOTIC SHOCK IN SYNECHOCOCCUS TRANSFORMANT

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Chinese Journal of Oceanology and Limnology 1998年第S1期16卷 105-108页

作者： shin-ichi yano Yoshikazu Kawata Hiroyuki Kojima(Osaka National Research Institute, Agency of Industrial Science and Technology, Ikeda, Osaka 563, Japan )

A unicellular cyanobacterium Synechococcus sp. strain PCC 7002 was transformedwith plasmid pQL1, on which β-lactamase gene (bla) and β-galactosidase gene (lacZ) were *** transformant cells released β-lactamase into medium by an abrupt drop of osmotic pressure. This re-sult indicates that this cyanobacterium recognizes and processes the signal sequence of β-lactamase, andaccumulates the enzyme in periplasm. Repeated release of β-lactamase was possible by repeated osmoticshocks without impairing cell viability. On the other hand, most of the β-galactosidase remained in cyto-plasm under the osmotic shock.

关键词： Synechococcus perienzyme β-lactamase osmotic shock

Correlation between dose-volume parameters and rectal bleeding after 12 fractions of carbon ion radiotherapy for prostate cancer

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World Journal of Radiology 2024年第7期16卷 256-264页

作者： Takashi Ono Hiraku Sato Yuya Miyasaka Yasuhito Hagiwara Natsuko yano Hiroko Akamatsu Mayumi Harada Mayumi ichikawa Department of Radiation Oncology Faculty of MedicineYamagata UniversityYamagata 990-9585Japan Department of Heavy Particle Medical Science Yamagata University Graduate School of Medical ScienceYamagata 990-9585Japan

BACKGROUND Carbon ion radiotherapy(CIRT)is currently used to treat prostate *** bleeding is a major cause of toxicity even with ***,to date,a correlation between the dose and volume parameters of the 12 fractions of CIRT for prostate cancer and rectal bleeding has not been ***,the clinical risk factors for rectal bleeding were absent after 12 fractions of *** To identify the risk factors for rectal bleeding in 12 fractions of CIRT for prostate *** Among 259 patients who received 51.6 Gy[relative biological effectiveness(RBE)],in 12 fractions of CIRT,15 had grade 1(5.8%)and nine had grade 2 rectal bleeding(3.5%).The dose-volume parameters included the volume(cc)of the rectum irradiated with at least x Gy(RBE)(Vx)and the minimum dose in the most irradiated x cc normal rectal volume(Dx).RESULTS The mean values of D6cc,D2cc,V10 Gy(RBE),V20 Gy(RBE),V30 Gy(RBE),and V40 Gy(RBE)were significantly higher in the patients with rectal bleeding than in those *** cutoff values were D6cc=34.34 Gy(RBE),D2cc=46.46 Gy(RBE),V10 Gy(RBE)=9.85 cc,V20 Gy(RBE)=7.00 cc,V30 Gy(RBE)=6.91 cc,and V40 Gy(RBE)=4.26 *** D2cc,V10 Gy(RBE),and V20 Gy(RBE)cutoff values were significant predictors of grade 2 rectal *** The above dose-volume parameters may serve as guidelines for preventing rectal bleeding after 12 fractions of CIRT for prostate cancer.

关键词： Carbon ion radiotherapy Prostate cancer Rectal bleeding Dose volume parameters Prevention

Effect of Intertidal Elevation at Tsuyazaki Cove,Fukuoka,Japan on Survival Rate of Horseshoe Crab Tachypleus tridentatus Eggs

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Journal of Ocean University of China 2022年第3期21卷 601-610页

作者： ITAYA shinji SHUUNO Mari ONIKURA Norio TAI Akira yano shinichiro Environmental Fluid Dynamics Laboratory Department of Urban and Environmental EngineeringGraduate School of EngineeringKyushu UniversityFukuoka819-0395Japan Tsuyazaki Seaside Nature School Fukutsu811-3304Japan Fishery Research Laboratory Department of Bioresource ScienceGraduate School of Bioenvironmental ScienceKyushu UniversityFukuoka819-0395Japan

Physical factors affecting the survival of Tachypleus tridentatus eggs were investigated by translocating their eggs between the high intertidal zone and the low intertidal zone of a known spawning *** mean egg survival rates per day were highest in the mid intertidal zone(45.1%±25.4%)and the lowest in the low intertidal zone(13.3%±27.6%).Differences in the elevation,air exposure time,and water content of the spawning ground were significant factors determining the egg survival *** or insufficient air exposure time resulted in inadequate water content at higher and lower intertidal zones and could reduce egg *** the other hand,moderate saturation and dehydration were repeated with each tidal movement in the mid intertidal *** dynamic is considered as one of the crucial factors for the survival of eggs and is considered optimal for ***,the protection of the mid intertidal zone is imperative for maximizing the egg survival rate in Tsuyazaki Cove where almost all suitable nesting sites have disappeared due to coastal *** protecting these optimal sites for spawning and recovering other optimal sites on suitable beaches,a positive contribution can be made to future management and *** study also suggests that translocating eggs from marginal to optimal spawning sites might be a recovery strategy for this globally endangered species.

关键词： air exposure time globally endangered species intertidal elevation intertidal zone optimal spawning site Tachypleus tridentatus translocated horseshoe crab eggs Tsuyazaki Cove

Evapotranspiration from citrus trees growing in sandy soil under drip irrigation with saline water

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Science China Earth Sciences 2002年第Z1期45卷 41-46页

作者：杨胜利 shin-ichi Takeuchi Tomohisa yano Yoshinobu Kitamura Arid Land Research Center Tottori University 1390 Hamaska Tottori 680-0001 Japan Faculty of Engineering Kyushu Kyouritsu University Kitakyushu 807-8585 Japan Faculty of Agriculture Tottori University Tottori 680-8553 Japan

An experiment on evapotranspiration from citrus trees under irrigation with saline waterwas carried out for 4 months. Two lysimeters planted with a citrus tree in the green house wereused. One lysimeter was irrigated with saline water (NaCl and CaCl2 of 2000 mg/L equivalence,EC = 3.8 dS/m, SAR = 5.9) and the other was irrigated with freshwater using drip irrigation. Theapplied irrigation water was 1.2 times that of the evapotranspiration on the previous *** was calculated as the change in lysimeter weight recorded every 30 *** lysimeters were filled with soil with 95.8% sand. The results of the experiment were as follows.(i) The evapotranspiration from citrus tree was reduced after irrigation with saline water. Theevapotranspiration returns to normal after leaching. However it takes months to exhaust the saltfrom the tree. ( ii ) To estimate the impact of irrigation with saline water on the evapotranspirationfrom citrus trees, the reduction coefficient due to salt stress (Ks) was used in this *** under irrigation with saline water (ETs) can be calculated from evapotranspira-tion under irrigation with freshwater (ET) by the equation ETs = Ks× ET. Ks can be expressed as afunction of ECsw. (iii) The critical soil-water electrical conductivity (ECsw) is 9.5 dS/m, beyondwhich adverse effects on evapotranspiration begin to appear. If ECsw can be controlled at below9.5 dS/m, saline water can be safely used for irrigation.

关键词： lysimeter, drip irrigation, saline water, reduction coefficient due to salt stress, evapotranspiration.

Crowd evacuation of pairs of pedestrians

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Communications in Theoretical Physics 2022年第4期74卷 97-112页

作者： Ryosuke yano Tokio Marine d R Co.ltd. 1-5-1OtemachiChiyoda-kuTokyo100-0004Japan

The crowd evacuation of pairs of pedestrians(*** consisting of a parent and a child)is numerically ***,it is assumed that all pedestrians have their own partners,and move randomly inside the bounded domain of the right-hand room as an initial *** pedestrians start their evacuations after they contact their *** evacuations are completed by the transfer of all the pairs from the right-hand room to the left-hand room through an exit.A frozen swarm tends to appear in the right-hand room as the total number of pedestrians *** frozen swarm moves without changing its form,unless it is dissolved by a strong collision with a pair of pedestrians that comes back from the left-hand room by ***,the evacuation speed also depends on the area of the Escape Zone,whereas an obstacle placed in front of an exit also changes the speed of the evacuation in accordance with the type of motion of the children.

关键词： Evacuation of paired pedestrians force chain network escape from crowd

Ongoing Applicative Studies of Plant Thioredoxins

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Molecular Plant 2014年第1期7卷 4-13页

作者： Hiroyuki yano National Food Research Institute National Agriculture and Food Research Organization Kannondai 2-1-12 Tsukuba Ibaraki 305-8642 Japan

Studies triggered by the discovery of the function of thioredoxin （Trx） in photosynthesis have revealed its role throughout biology. Parallel biochemical and proteomic analyses have led to the identification of its numerous puta- tive targets. Recently, to verify the biological significance of these targets, in vivo studies using transformants in which Trx is overexpressed or suppressed are in progress, and the transformants themselves that are being used in such studies show their potential applicative values. Moreover, Trx＇s mitigation of allergenicity for some proteins offers promising prospects in the food industry. Practical studies based on redox regulation, once only on the horizon, are now achieving new dimensions. This short review focuses on the industrial applications of Trx studies, the current situation, and future perspectives. The putative targets obtained by the proteomics approach in comparison with in vivo observations of the transformants are also examined. Applicative studies of glutathione, a counterpart of Trx, are also discussed briefly.

关键词： allergen food redox regulation target thioredoxin.

INTELLIGENT METATAL COMPLEXES CONTAINING N-GLYCOSIDES FORMED FROM TRIS (2-AMINOETHYL) AMINE AND ALDOSES, HAVING MOLECULAR RECOGNITION ABILITY

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Chinese Journal of Polymer Science 1998年第3期16卷 193-206页

作者： yano Shigenobu Department of Chemistry Nara Women's University Kitauoyanishimachi Nara 630-8263 Japan

Assembly of carbohydrates on nickel (Ⅱ) center by utilizing N-glycosidicbond formation with a branched amine: tris(2-aminoethyl)amine (tren), an unprecedentedchiral inversion around the metal center (Co or Mn) induced by an interaction betweensugars and sulfate anions, peroxo-bridged dinuclear cobalt (Ⅲ) complex containing N-glycoside ligands from tren and D-glucose and its reversible dioxygen binding property,and novel trimanganese complexes with a linear Mn_3 (Ⅱ, Ⅲ, Ⅱ) assemblage bridged bycarbohydrates are described.

关键词： Intelligent sugar complex Molecular recognition N-glycoside complex

Effects of docosahexaenoic acid or arachidonic acid supplementation on the behavior of cardiomyocytes derived from human pluripotent stem cells

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BIOCELL 2023年第5期47卷 1095-1106页

作者： MIZUNA yano KOTA HIROI TETSUYA YUASA KENJI INOUE OSAMU YAMAMOTO TAKAO NAKAMURA DAISUKE SATO ZHONGGANG FENG Graduate School of Science and Engineering Yamagata UniversityYonezawa9928510Japan Faculty of Health Sciences Japan Healthcare UniversitySapporo0620053Japan

Background:Human heart changes its energetic substrates from lactate and glucose to fatty acids during the neonatal *** the lack of fatty acids in media for the culture of cardiomyocytes derived from human pluripotent stem cells(hiPS-CM),researchers have supplemented mixtures of fatty acids to hiPS-CM and reported the enhancement in the maturation of *** our previous studies,we separately supplemented two polyunsaturated fatty acids(PUFAs),docosahexaenoic acid(DHA)or arachidonic acid(AA),to rat fetal cardiomyocytes and found that the supplementations upregulated the expressions of mRNAs for cardiomyocyte differentiation,fatty acid metabolism,and cellular *** enhancement in cellular contractility was attributed to the improvement in intercellular connection rather than a direct enhancement of the contractile ***:This study reports the successive results of the effects of DHA or AA supplementation on *** addition to the contractile force and mRNA measurements used in the previous study,we further investigated the effect of different cellular aggregations on the contractile force output by means of finite element analysis,measured glucose and fatty acids metabolites,and assessed cTNT and MLC2v expressions through immunofluorecsence ***:It showed that the sole supplementation of albumin-conjugated DHA or AA can be taken up by hiPS-CM without other uptake-enhancing factors,and the supplementations may activate the CD36_ERRγmetabolic *** or AA supplementation increased the cellular contractile ratio on collagen gels and AA supplementation stimulated hiPS-CM aggregation to form cellular *** enhancement effect on the hiPS-CM contractile force was modest since the increase in contractile force was not *** supplementation was more effective than DHA supplementation because it significantly upregulated mRNA expressions of P300 and ***,finite element analysis showed that the formati

关键词： Human iPS cells Cardiomyocytes Polyunsaturated fatty acid mRNA expression Contractility Fatty acid metabolism