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High-resolution genetic mapping and identification of candidate genes for the wheat stem rust resistance gene Sr8155B1

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The Crop Journal 2023年第6期11卷 1852-1861页

作者： Jian Wang Hongyu Li Tao Shen shikai lyu Shams ur Rehman Hongna Li Guiping Wang Binyang Xu Qing Wang Wanyi Hu Kairong Li Shengsheng Bai Jian Ma Haitao Yu Matthew N.Rouse Shisheng Chen School of Advanced Agriculture Sciences Peking UniversityBeijing 100871China National Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural SciencesShandong Laboratory of Advanced Agricultural Sciences in WeifangWeifang 261325ShandongChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina Wheat Research Institute Weifang Academy of Agricultural SciencesWeifang 261071ShandongChina US Department of Agriculture-Agricultural Research Service Cereal Disease Laboratory and Department of Plant PathologyUniversity of MinnesotaSt.PaulMN 55108USA

Stem rust,caused by Puccinia graminis ***(Pgt),threatens global wheat *** of cultivars with increased resistance to stem rust by identification,mapping,and deployment of resistance genes is the best strategy for controlling the *** this study,we performed fine mapping and characterization of the all-stage stem rust resistance(Sr)gene Sr8155B1 from the durum wheat line *** seedling tests of biparental populations,Sr8155B1 was effective against six Chinese Pgt races *** a segregating population of 5060 gametes,Sr8155B1 was mapped to a 0.06-cM region flanked by markers Pku2772 and Pku43365,corresponding to 1.5-and 2.7-Mb regions in the Svevo and Chinese Spring reference *** regions include several typical nucleotide-binding leucine-rich repeat(NLR)and protein kinase genes that represent candidate *** them,three NLR genes and three receptor-like protein kinases were highly polymorphic between the parental lines and their transcripts were upregulated in the homozygous resistant line TdR2 relative to its susceptible sister line *** markers(Pku2772,Pku43365,Pku2950,and Pku3721)developed in this study,together with seedling resistance responses,correctly predicted Sr8155B1 absence or presence in 78 tetraploid wheat genotypes *** presence of Sr8155B1 in tetraploid wheat accessions CItr 14916,PI 197492,and PI 197493 was confirmed by mapping in three F_(2)*** genetic map and linked markers developed in this study may accelerate the deployment of Sr8155B1-mediated resistance in wheat breeding programs.

关键词： Durum wheat Stem rust Resistance gene Sr8155B1 CC-NBS-LRR

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An orchestrated ethylene-gibberellin signaling cascade contributes to mesocotyl elongation and emergence of rice direct seeding

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Journal of Integrative Plant Biology 2024年第7期66卷 1427-1439页

作者： Yusong lyu Xinli Dong Shipeng Niu Ruijie Cao Gaoneng Shao Zhonghua Sheng Guiai Jiao Lihong Xie shikai Hu Shaoqing Tang Xiangjin Wei Peisong Hu State Key Laboratory of Rice Biology and Breeding China National Center for Rice ImprovementChina National Rice Research InstituteHangzhou 310006China College of Agriculture and Biotechnology Zhejiang UniversityHangzhou 310058China

A mechanized direct seeding of rice with less labor and water usage,has been widely ***,this approach requires varieties that exhibit uniform seedling *** elongation(ME)offers the main drive of fast emergence of rice seedlings from soils;nevertheless,its genetic basis remains ***,we identify a major rice quantitative trait locus Mesocotyl Elongation1(qME1),an allele of the Green Revolution gene Semi-Dwarf1(SD1),encoding GA20-oxidase for gibberellin(GA)***1 expression is strongly induced by soil depth and *** rice grains are direct-seeded in soils,the ethylene core signaling factor OsEIL1 directly promotes ME1 transcription,accelerating bioactive GA *** GAs further degrade the DELLA protein SLENDER RICE 1(SLR1),alleviating its inhibition of rice PHYTOCHROME-INTERACTING FACTOR-LIKE13(OsPIL13)to activate the downstream expansion gene OsEXPA4 and ultimately promote rice seedling ME and *** ancient traits of long mesocotyl and strong emergence ability in wild rice and landrace were gradually lost in company with the Green Revolution dwarf breeding process,and an elite ME1-R allele(D349H)is found in some modern Geng varieties(long mesocotyl lengths)in northern China,which can be used in the direct seeding and dwarf breeding of Geng ***,the ectopic and high expression of ME1 driven by mesocotyl-specific promoters resulted in rice plants that could be direct-seeded without obvious plant architecture or yield ***,we reveal the molecular mechanism of rice ME,and provide useful information for breeding new Green Revolution varieties with long mesocotyl suitable for direct-seeding practice.

关键词： rice(Oryza sativa L.) direct seeding Green Revolu-tion mesocotyl elongation ethylene gibberellin semi-dwarf1(SD1)

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IHVFL: a privacy-enhanced intention-hiding vertical federated learning framework for medical data

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Cybersecurity 2024年第2期7卷 96-112页

作者： Fei Tang shikai Liang Guowei Ling Jinyong Shan College of Computer Science and Technology Chongqing University of Posts and TelecommunicationsNo.2Chongwen RoadNan'an DistrictChongqing 400065China School of Cyber Security and Information Law Chongqing University of Posts and TelecommunicationsChongqing 400065China sudo Technology Co. LTD.Beijing 100083China

Vertical Federated Learning(VFL)has many applications in the field of smart healthcare with excellent ***,current VFL systems usually primarily focus on the privacy protection during model training,while the preparation of training data receives little *** real-world applications,like smart healthcare,the process of the training data preparation may involve some participant's intention which could be privacy information for this *** protect the privacy of the model training intention,we describe the idea of Intention-Hiding Vertical Feder-ated Learning(IHVFL)and illustrate a framework to achieve this privacy-preserving ***,we construct two secure screening protocols to enhance the privacy protection in feature ***,we implement the work of sample alignment bases on a novel private set intersection ***,we use the logistic regression algorithm to demonstrate the process of *** show that our model can perform better efficiency(less than 5min)and accuracy(97%)on Breast Cancer medical dataset while maintaining the intention-hiding goal.

关键词： Medical data Vertical federated learning Privacy-presserving Intention-hiding Logistic regression

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Non-Targeted Metabolomics Reveals the Metabolic Alterations in Response to Artificial Selective Breeding in the Fast-Growing Strains of Pacific Oyster

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Journal of Ocean University of China 2024年第2期23卷 518-528页

作者： HU Boyang TIAN Yuan LIU shikai LI Qi Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Laoshan LaboratoryQingdao 266237China

Pacific oyster(Crassostrea gigas)is one of the most important mollusks cultured all around the *** breeding programs of Pacific oysters in China is initiated since 2006 and developed the genetically improved strain with fast-growing ***,little is known about the metabolic signatures of the fast-growing *** the present study,the non-targeted metabolomics was performed to analyze the metabolic signatures of adductor muscle tissue in one-year old Pacific oysters from fast-growing strain and the wild population.A total of 7767 and 10174 valid peaks were extracted and quantified in ESI^(+)and ESI^(−)modes,resulting in 399 and 381 annotated metabolites,*** and OPLS-DA revealed that considerable separation among samples from fastgrowing strain and wild population,suggesting the differences in metabolic ***,81 significantly different metabolites(SDMs)were identified in the comparisons between fast-growing strain and wild population,based on the strict *** was found that there were highly correlation and conserved coordination among these *** enrichment analysis indicated that the SDMs were tightly related to pantothenate and CoA biosynthesis,steroid hormone biosynthesis,riboflavin metabolism,and arginine and proline *** them,the CoA biosynthesis and metabolism,affected by pantetheine and pantothenic acid,might be important for the growth of Pacific oysters under artificial selective *** study provides the comprehensive views of metabolic signatures in response to artificially selective breeding,and is helpful to better understand the molecular mechanism of fastgrowing traits in Pacific oysters.

关键词： metabolic signature Pacific oyster artificial selection fast-growing trait

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2’-Fucosyllactose alleviate immune checkpoint blockade-associated colitis by reshaping gut microbiota and activating AHR pathway

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Food Science and Human Wellness 2024年第5期13卷 2543-2561页

作者： shikai Yan Leilei Yu Fengwei Tian Jianxin Zhao Wei Chen Qixiao Zhai State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China National Engineering Research Center for Functional Food Jiangnan UniversityWuxi 214122China

Immune checkpoint blockade(ICB)therapeutics are highly effective in cancer immunotherapy,but gastrointestinal toxicity limited the *** microbiota plays a crucial role in ICB-associated colitis.2’-Fucosyllactose(2’FL)is most abundance prebiotic in human milk that can reshape gut microbiota and exert immune regulatory *** study aimed to determine the effects of 2’FL on ICB-associated colitis and to uncover the mediating ***-associated colitis was induced by the ipilimumab and dextran sulfate *** administration of 2’FL(0.6 g/(kg∙day))ameliorated ICB-induced colitis by enhancing regulatory T cells(Treg)and the M2/M1 ratio of macrophages in colon.2’FL treatment also increased the expression of tight junction proteins(zonula occludens-1(ZO-1)and mucin 2(MUC2))and antioxidant stress indicators(superoxide dismutase(SOD)and catalase(CAT)).In addition,administration of 2’FL increased the abundance of Bifidobacterium and Lactobacillus,and elevated the levels of microbial metabolites,such as indole-3-lactic acid(ILA),which activated the aryl hydrocarbon receptor ligands(AHR)*** protective effect of 2’FL was abolished upon depletion of gut microbiota,and ILA treatment partially simulated the protective effect of 2’***,2’FL did not exhibit inhibition of antitumor *** findings suggest that 2’FL could serve as a potential protective strategy for ICB-associated colitis by modulating the intestinal microbiota and bacterial metabolites.

关键词： 2’-Fucosyllactose Immune checkpoint blockade-associated colitis Gut microbiota Metabolomics Regulatory T cells(Treg)

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中国共产党面对的大党独有难题及其应对之道

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社会科学 2024年第5期 5-20,84页

作者：汪仕凯复旦大学国际关系与公共事务学院

中国共产党是由先进分子组成的先锋队,在领导革命和治国理政的过程中,党发展成超大规模的先锋队。中国共产党的规模对先锋队性质的影响以及对于党完成历史使命的制约,是中国共产党面对的大党独有难题。更进一步地说,中国共产党如何在保... 详细信息

中国共产党是由先进分子组成的先锋队,在领导革命和治国理政的过程中,党发展成超大规模的先锋队。中国共产党的规模对先锋队性质的影响以及对于党完成历史使命的制约,是中国共产党面对的大党独有难题。更进一步地说,中国共产党如何在保持超大规模的同时抑制住超大规模对于先锋队性质的可能侵蚀,并且在实现党的先锋队性质与超大规模之间平衡的基础上,能够始终赢得人民拥护、巩固长期执政地位,从而完成历史使命,则是中国共产党面对的大党独有难题的深层内涵。中国共产党从其发展成超大规模先锋队时就面对着大党独有难题,同样也开始了解决大党独有难题的历史实践,民主集中制是有效解决大党独有难题的制度基础,党中央权威和集中统一领导则体现了这个制度基础的深刻之处。以政治建设统领党的建设伟大工程、通过自我革命推进全面从严治党、以自我革命带动社会革命、依规治党与依法治国相统一,则是中国共产党在新时代有效解决大党独有难题的根本之道。

关键词：中国共产党大党独有难题先锋队民主集中制自我革命

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A High Efficiency Hardware Implementation of S-Boxes Based on Composite Field for Advanced Encryption Standard

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Journal of Computer and Communications 2024年第4期12卷 228-246页

作者： Yawen Wang Sini Bin shikai Zhu Xiaoting Hu College of Computer Science & Technology Jiangsu Normal University Xuzhou China

The SubBytes (S-box) transformation is the most crucial operation in the AES algorithm, significantly impacting the implementation performance of AES chips. To design a high-performance S-box, a segmented optimization implementation of the S-box is proposed based on the composite field inverse operation in this paper. This proposed S-box implementation is modeled using Verilog language and synthesized using Design Complier software under the premise of ensuring the correctness of the simulation result. The synthesis results show that, compared to several current S-box implementation schemes, the proposed implementation of the S-box significantly reduces the area overhead and critical path delay, then gets higher hardware efficiency. This provides strong support for realizing efficient and compact S-box ASIC designs.

关键词： Advanced Encryption Standard (AES) S-Box Tower Field Hardware Implementation Application Specific Integration Circuit (ASIC)

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“商量政府”:中国式现代政府理论初探

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理论学刊 2024年第1期 86-95页

作者：汪仕凯复旦大学国际关系与公共事务学院上海200433

当代中国政府是由中国共产党和行政机关共同组成的,特殊的构成使中国共产党将其革命时期创造的成果即协商,作为一种基本要素带入到当代中国政府中来。从中国共产党领导治国理政的经验来看,协商贯穿于当代中国政府的结构、体制、机制以... 详细信息

当代中国政府是由中国共产党和行政机关共同组成的,特殊的构成使中国共产党将其革命时期创造的成果即协商,作为一种基本要素带入到当代中国政府中来。从中国共产党领导治国理政的经验来看,协商贯穿于当代中国政府的结构、体制、机制以及过程中,从而使当代中国政府呈现出不同于一般现代政府的特性。并且,当代中国政府所具有的协商特性事实上构成了其优势,中国发展的巨大成就证明当代中国政府能够将其优势转化为治理效能。根据当代中国政府的特性和优势,“商量政府”是对当代中国政府进行的理论化表达,代表着一种对中国式现代政府理论的探索。

关键词：商量政府中国共产党政府理论

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基于混合维修机制的动车组多设备系统维修策略优化

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铁道运输与经济 2024年第10期46卷 200-206页

作者：齐群熊律严世开李晶田鹏广东交通职业技术学院轨道交通学院广东广州510650 兰州交通大学机电工程学院甘肃兰州730070 中国铁路广州局集团有限公司安全监察室广东广州528478

为降低动车组多设备系统一个寿命周期内的维修总成本,以动车组某设备系统为研究对象,采用混合式故障率演化规则描述其故障率变化,以预防性维修成本和故障维修成本为主要成本考量,对其一个寿命周期内的维修经济性进行优化研究,并结合我... 详细信息

为降低动车组多设备系统一个寿命周期内的维修总成本,以动车组某设备系统为研究对象,采用混合式故障率演化规则描述其故障率变化,以预防性维修成本和故障维修成本为主要成本考量,对其一个寿命周期内的维修经济性进行优化研究,并结合我国动车组维修实际,分别建立等周期预防性维修策略和基于可靠性的预防性维修策略模型。通过分析动车组系统中各单设备在两种维修策略下的维修总成本,选择使维修总成本最低的维修策略作为该设备一个寿命周期内的最终维修策略,进而提出一种动车组多设备系统混合维修策略。算例结果表明,混合维修策略下动车组多设备系统的维修总成本相较于等周期预防性维修策略降低了19.3%,相较于基于可靠性的预防性维修策略降低了16.7%。

关键词：动车组多设备系统等周期可靠性混合维修

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超声辅助对摆动激光熔丝增材制造2319铝合金沉积成形和显微组织的影响行为

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中国激光 2024年第10期51卷 217-230页

作者：王燕霜张震吴世凯齐鲁工业大学(山东省科学院)机械工程学院山东济南250353 齐鲁工业大学(山东省科学院)激光研究所山东济南250353

为解决高强铝合金激光增材制造过程中存在的孔洞缺陷、组织不均、晶粒粗大等难题,在摆动激光熔丝沉积的基础上引入超声能场是可行的抑制措施。搭建了超声辅助摆动激光熔丝增材制造试验平台,探究了超声功率、变幅杆压力、变幅杆输入位置... 详细信息

为解决高强铝合金激光增材制造过程中存在的孔洞缺陷、组织不均、晶粒粗大等难题,在摆动激光熔丝沉积的基础上引入超声能场是可行的抑制措施。搭建了超声辅助摆动激光熔丝增材制造试验平台,探究了超声功率、变幅杆压力、变幅杆输入位置对2319铝合金沉积层形貌和显微组织的影响规律并对超声工艺参数进行了优化。结果表明:变幅杆压力对沉积宏观形貌具有显著影响,随着压力的增大熔池横截面形貌逐渐趋于上宽下窄;超声参数对单道沉积层的晶粒尺寸有着明显影响,随着超声功率的增加晶粒尺寸先减小后增大,当超声功率占比(超声波发生器的输出功率占其最大输出功率的百分比)为40%时晶粒尺寸达到最小值70.9μm。超声输入位置在熔池之后时晶粒细化效果更好,且在-10 mm处晶粒尺寸同组最低,只有80.9μm。当变幅杆压力处于40~100 N范围内时晶粒的细化效果比较明显,晶粒尺寸在110~130μm之间。观察多层沉积结果可知,超声的引入使得多层样品的平均晶粒尺寸降低了66.3%、增加了Al2Cu强化相的含量并且抑制了Al的滑移面生长趋势。超声在熔池中传递所产生的声流效应和空化效应是使沉积层成分均匀、缺陷减少和组织细化的主要原因。

关键词：激光技术摆动激光超声辅助 2319铝合金增材制造显微组织

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