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Bispecific aptamer-initiated 3D DNA nanomotor biosensor powered by DNAzyme and entropy-driven circuit for sensitive and specificity detection of lysozyme

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Nano Research 2023年第1期16卷 1286-1295页

作者： Shuo Qi Yuhan Sun Xiaoze Dong Imran Mahmood Khan Yan Lv Yin Zhang Nuo Duan Shijia Wu Zhouping Wang State Key Laboratory of Food Science and Technology International Joint Laboratory on Food SafetyJiangnan UniversityWuxi 214122China School of Food Science and Technology Jiangnan UniversityWuxi 214122China Key Laboratory of Meat Processing of Sichuan Chengdu UniversityChengdu 610106China National Engineering Research Center for Functional Food Jiangnan UniversityWuxi 214122China Collaborative Inno vation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityWuxi 214122China

Dynamic DNA nanodevices have gained tremendous attention due to their extraordinary inherent functionality and advantages,however,dynamic DNA nanodevices-based biosensors are still challenging due to their high reliance on proteases and limited amplification capabilities.Herein,exploiting bispecific aptamer as initiators for the first time,we developed a three-dimensional(3D)DNA nanomotor biosensor powered by DNAzyme and entropy-driven circuit for sensitive and specific detection of lysozyme,in which walking and rolling strategies are efficiently integrated to achieve excellent signal amplification capability.Benefiting from the high selectivity of bispecific aptamer,the 3D DNA nanomotor biosensor can respond to lysozyme with high specificity and operate at high speed to release signals.The whole process is independent of protease,avoiding the influence of adverse environment on the operation stability.Under optimal conditions,it can achieve a limit of detection as low as 0.01 pg/mL with an excellent linear range of 0.05 pg/mL–500 ng/mL for lysozyme.Furthermore,the proposed strategy revealed high accuracy in the analysis of real samples,indicating a great potential for the application of nanomotor biosensors to the detection of non-nucleic acid targets.

关键词： bispecific aptamer DNA rolling DNA walking amplification specificity

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γ-MnO2 nanorods/graphene composite as efficient cathode for advanced rechargeable aqueous zinc-ion battery

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Journal of Energy Chemistry 2020年第4期29卷 182-187页

作者： Chao Wang Yinxiang Zeng Xiang Xiao Shijia Wu Guobin Zhong Kaiqi Xu Zengfu Wei Wei Su Xihong Lu Electric Power Research Institute of Guangdong Power Grid Co. Ltd.Guangzhou 510080GuangdongChina MOE of the Key Laboratory of Bioinorganic and Synthetic Chemistry The Key Lab of Low-Carbon Chem&Energy Conservation of Guangdong ProvinceSchool of ChemistrySun Yat-Sen UniversityGuangzhou 510275GuangdongChina

Aqueous Zn//MnO2 batteries are emerging as promising large-scale energy storage devices owing to their cost-effectiveness,high safety,high output voltage,and energy density.However,the MnO2 cathode suffers from intrinsically poor rate performance and rapid capacity deterioration.Here,we remove the roadblock by compositing MnO2 nanorods with highly conductive graphene,which remarkably enhances the electrochemical properties of the MnO2 cathode.Benefiting from the boosted electric conductivity and ion diffusion rate as well as the structural protection of graphene,the Zn//MnO2-graphene battery presents an admirable capacity of 301 mAh g^-1 at 0.5 A g^-1,corresponding to a high energy density of 411.6 Wh kg^-1.Even at a high current density of 10 A g^-1,a decent capacity of 95.8 mAh g^-1 is still obtained,manifesting its excellent rate property.Furthermore,an impressive power density of 15 kW kg^-1 is achieved by the Zn//MnO2-graphene battery.

关键词： γ-MnO2 Graphene Zinc-ion battery High-capacity cathode

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Facile cyclic ammonium salt with the smallest size for high performance electric double layer capacitors

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Chinese Chemical Letters 2019年第6期30卷 1269-1272页

作者： Chao Wang Jing Wang Xiang Xiao Guobin Zhong Shijia Wu Kaiqi Xu Wei Zhao Wei Su Jie Zeng Baojun wu Weili Zhang Changcheng wu Zhiqiang Shi Electric Power Research Institute of Guangdong Power Grid Co. Ltd. Guangzhou 510080 China Tianjin Key Laboratory of Advanced Fibers and Energy Storage College of Materials Science and Engineering Tianjin Polytechnic University Tianjin 300387 China

The smallest cyclic ammonium salt reported to date, N,N-dimethylpyrrolidinium tetrafluoroborate (P11-BF4), was successively synthesized using a synthesis route without metal ions and halogen ions, then investigated as the electrolyte with Propylene carbonate in EDLCs. The electrochemical characteristics of EDLCs assembled by 1 mol/L P11-BF4/PC paired with activated carbon electrodes were compared to traditional electrolytes. P11-BF4 has proven to have superior voltage resistance by using cyclic voltammetry and constant current charge-discharge testing. Moreover, P11-BF4 exhibits a more brilliant rate performance due to its high conductivity. These results demonstrate that P11-BF4 is an ideal electrolyte to improve the energy density and power density of supercapacitors.

关键词： N N-Dimethylpyrrolidinium tetrafluoroborate Cyclic ammonium salt Electrical conductivity Supercapacitor Rate performance

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Design and fabrication of r-hirudin loaded dissolving microneedle patch for minimally invasive and long-term treatment of thromboembolic disease

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Asian Journal of Pharmaceutical Sciences 2022年第2期17卷 284-297页

作者： Mengfang wu Tian Xia Yaran Li Tianfa Wang shijia Yang Jinchao Yu Qiaoyan Liang Teng Shen Min Yu Bing Zhao Key Laboratory of Metabolism and Molecular Medicine Ministry of EducationFudan UniversityShanghai 200032China The Department of Biochemistry and Molecular Biology School of Basic Medical SciencesFudan UniversityShanghai 200032China Key Laboratory of Smart Drug Delivery Ministry of EducationDepartment of PharmaceuticsSchool of PharmacyFudan UniversityShanghai 201203China

Cardiovascular disease is the leading cause of global mortality,with anticoagulant therapy being the main prevention and treatment strategy.Recombinant hirudin(r-hirudin)is a direct thrombin inhibitor that can potentially prevent thrombosis via subcutaneous(SC)and intravenous(IV)administration,but there is a risk of haemorrhage via SC and IV.Thus,microneedle(MN)provides painless and sanitary alternatives to syringes and oral administration.However,the current technological process for the micro mould is complicated and expensive.The micro mould obtained via three-dimensional(3D)printing is expected to save time and cost,as well as provide a diverse range of MNs.Therefore,we explored a method for MNs array model production based on 3D printing and translate it to micro mould that can be used for fabrication of dissolving MNs patch.The results show that r-hirudin-loaded and hyaluronic acid(HA)-based MNs can achieve transdermal drug delivery and exhibit significant potential in the prevention of thromboembolic disease without bleeding in animal models.These results indicate that based on 3D printing technology,MNs combined with r-hirudin are expected to achieve diverse customizableMNs and thus realize personalized transdermal anticoagulant delivery for minimally invasive and long-term treatment of thrombotic disease.

关键词： Anticoagulant r-hirudin Microneedle patch 3D printing technology

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基于物联终端的工作面甲烷数字场实现方法研究

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煤炭科学技术 2021年第12期49卷 112-118页

作者：屈世甲吴雪菲中煤科工集团常州研究院有限公司江苏常州213015 天地(常州)自动化股份有限公司江苏常州213015 西安科技大学能源学院陕西西安710054 中煤科工集团西安研究院有限公司陕西西安710077

从系统监测和人工检测2个方面对现有综采工作面甲烷监测现状进行了分析,针对工作面现有甲烷传感器体积、功耗大,移动、维护困难和设备成本高等问题,在物联网技术和边缘计算理念的基础上,提出了利用微功耗甲烷数据采集、微功耗传感终端... 详细信息

从系统监测和人工检测2个方面对现有综采工作面甲烷监测现状进行了分析,针对工作面现有甲烷传感器体积、功耗大,移动、维护困难和设备成本高等问题,在物联网技术和边缘计算理念的基础上,提出了利用微功耗甲烷数据采集、微功耗传感终端定位、无线数据传输和终端自供电4种关键技术和开发微功耗甲烷感知终端的思路,并提出了4种关键技术的具体技术指标和性能要求。针对监控系统工作面区域甲烷监测点少,人工检测甲烷数据实时性、可读性和共享性不足的问题,以工作面区域落煤甲烷涌出、煤壁甲烷涌出和采空区甲烷涌出三大主要涌出特征为基础,结合现场生产班和检修班的实测甲烷数据分布特征,提出了综采工作面区域按照26台物联甲烷感知终端布置方案。在26个甲烷实时监测数据点基础上,为更直观、清楚地展示工作面区域甲烷分布状态,对有限的甲烷监测数据进行插值,拟形成工作面区域甲烷浓度数字场。在笛卡尔二维坐标系下分别优选了工作面区域X和Y方向的数字场实现方法并进行了验证,结果表明该方法大幅提高了工作面区域甲烷监测数据的可读性和实时性,为进一步做区域甲烷分析预警提供了实时性高、覆盖面广、可读性强的工作面甲烷浓度表达方式。

关键词：物联网甲烷感知物联终端甲烷浓度数字场

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IP时代下的电视播出系统架构思考

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电视技术 2023年第11期47卷 49-53,57页

作者：吴世佳海口广播电视台海南海口570126

随着4K、8K超高清技术的出现、信息技术(Information Technology,IT)的迅猛发展、虚拟化技术及基于网际互连协议(Internet Protocol,IP)网络的传输标准日益完善,电视系统传统的串行数字接口(Serial Digital Interface,SDI)技术架构在带... 详细信息

随着4K、8K超高清技术的出现、信息技术(Information Technology,IT)的迅猛发展、虚拟化技术及基于网际互连协议(Internet Protocol,IP)网络的传输标准日益完善,电视系统传统的串行数字接口(Serial Digital Interface,SDI)技术架构在带宽、互联网应用、灵活配置等方面显得越来越力不从心。虚拟化技术、云技术以海量的带宽、灵活的资源配置方式、多样的兼容性为电视播出系统的架构提供了新的思路。基于此,阐述电视播出系统架构的过去、现在、未来,对今后电视播出系统架构的选择进行深层次的思考。

关键词：串行数字接口(SDI) 切换台云播出

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基于边缘计算的采煤工作面甲烷监测模式研究

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煤炭科学技术 2020年第12期48卷 161-167页

作者：屈世甲武福生中煤科工集团常州研究院有限公司江苏常州213015 天地(常州)自动化股份有限公司江苏常州213015

针对工作面现有甲烷监测点不足,无法满足智慧矿山建设中工作面甲烷感知、分析和预警需求的问题,结合边缘计算技术、矿山物联网环境感知技术和数据传输等相关技术,分析了甲烷微功耗感知、自组网传输、监测终端供电和传感位置服务问题。... 详细信息

针对工作面现有甲烷监测点不足,无法满足智慧矿山建设中工作面甲烷感知、分析和预警需求的问题,结合边缘计算技术、矿山物联网环境感知技术和数据传输等相关技术,分析了甲烷微功耗感知、自组网传输、监测终端供电和传感位置服务问题。提出了集微功耗甲烷感知、自供电、自定位及自组网数据传输于一体的甲烷监测终端,配合工作面区域边缘网关、矿端边缘服务器和云端大数据分析,建立了基于云、边、端三级的甲烷边缘监测模式的系统架构,解决了工作面区域基于边缘计算的甲烷感知和传输问题。以100台液压支架、2台端头支架工作面为例,在笛卡尔二维坐标系下设计了该工作面基于边缘计算的甲烷监测方案,实现了甲烷浓度二维空间范围的大数据量监测,提出了工作面区域全覆盖的甲烷含量数字场实现方法和云图直观展示方式。在甲烷含量空间场和云图展示的基础上,提出了2种基于大数据支撑的甲烷含量分析预警模式和思路,一是对甲烷超限含量前后区域空间场进行关联分析,提取工作面甲烷含量超限对应空间场的分布特征和变化规律,利用区域含量场及早发现甲烷含量超限问题,另一种是利用大数据分析工具解决甲烷监测报警阈值以下的显现异常问题。

关键词：边缘计算边缘网关智慧矿山甲烷监测甲烷感知煤矿智能化

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煤矿安全监测监控体系中边缘计算模式研究

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煤炭科学技术 2022年第5期50卷 247-252页

作者：屈世甲武福生贺耀宜中煤科工集团常州研究院有限公司江苏常州213015 天地(常州)自动化股份有限公司江苏常州213015

针对煤矿现有监测监控体系下井下融合度不高,现场灾害分析和联动处理能力缺乏,无法满足监测监控深度融合需要的问题。对标智慧矿山建设中安全保障方面对监测监控体系的要求,论述了建立深度融合监测监控体系的必要性。分析了边缘计算技... 详细信息

针对煤矿现有监测监控体系下井下融合度不高,现场灾害分析和联动处理能力缺乏,无法满足监测监控深度融合需要的问题。对标智慧矿山建设中安全保障方面对监测监控体系的要求,论述了建立深度融合监测监控体系的必要性。分析了边缘计算技术在煤矿监测监控体系中的适用性,提出了建立基于云、边、端三级的煤矿监测监控体系架构,增加“端”侧传感信息的采集和第一级安全分析预警并实现区域联动功能。提出了煤矿监测监控体系中设置两级边缘节点的模式,分析指出边缘网关是当前更为关注的边缘计算节点。结合EdgeX Foundry平台架构对智能边缘网关的硬件平台和软件平台设计提出了构想,设计了煤矿安全监控系统边缘网关的整体架构。以煤矿工作面外因火灾为例,分析了现有监测监控体系和基于边缘计算监测监控体系下对火灾信息采集、分析预警和灾变处置的不同流程,将原有的火灾9步处理流程优化为4步处理流程,分析认为基于边缘计算的监测监控体系在系统井下深度融合、区域安全融合分析和处置方面相比较现有监测监控体系都有一定的优势。

关键词：边缘计算边缘网关监测监控数据分析火灾监测

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SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer

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Protein & Cell 2020年第7期11卷 483-504页

作者： shijia Bi Zunpeng Liu Zeming wu Zehua Wang Xiaoqian Liu Si Wang Jie Ren Yan Yao Weiqi Zhang Moshi Song Guang-Hui Liu Jing Qu State Key Laboratory of Stem Cell and Reproductive Biology Instiute of ZoologyChinese Academy of SciencesBejing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBejing 100101China Beiing Institute for Brain Disorders Advanced Innovation Center for Human Brain ProtectionNational Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China CAS Key Laboratory of Genomic and Precision Medicine Beiing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National Center for Bioinformation Bejing 100101China Institute for Stem cell and Regeneration Chinese Academy of SciencesBeiing 100101China University of Chinese Academy of Sciences Beijing 100049China Department of Cardiology Bejing Anzhen HospitalCapital Medical UniversityBeijing 100029China

SIRT7,a sirtuin family member implicated in aging and disease,is a regulator of metabolism and stress responses.It remains elusive how human somatic stem cell populations might be impacted by SIRT7.Here,we found that SIRT7 expression declines during human mesenchymal stem cell(hMSC)aging and that SIRT7 deficiency accelerates senescence.Mechanistically,SIRT7 forms a complex with nuclear lamina proteins and heterochromatin proteins,thus maintaining the repressive state of heterochromatin at nuclear periphery.Accordingly,deficiency of SIRT7 results in loss of heterochromatin,derepression of the LINE1 retrotransposon(LINE1),and activation of innate immune signaling via the cGAS-STING pathway.These agingassociated cellular defects were reversed by overexpression of heterochromatin proteins or treatment with a LINE1 targeted reverse-transcriptase inhibitor.Together,these findings highlight how SIRT7 safeguards chromatin architecture to control innate immune regulation and ensure geroprotection during stem cell aging.

关键词： SIRT7 stem cell aging LINE1 cGAS STING

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PA1426 regulates Pseudomonas aeruginosa quorum sensing and virulence:an in vitro study

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Journal of Bio-X Research 2021年第1期4卷 18-28页

作者： Fang Tan Pengfei She Linying Zhou shijia Li Xianghai Zeng Lanlan Xu Yaqian Liu Zubair Hussain Yong wu Department of Clinical Laboratory The Third Xiangya Hospital of Central South UniversityChangshaChina

Objective:Pseudomonas aeruginosa(P.aeruginosa)contains a hierarchy of quorum sensing(QS)network,consisting of the las,rhl and pqs systems,which play a key role in coordinating the expression of virulence factors.PA2146 was found to be associated with P.aeruginosa pathogenicity in macrophage and host Immune response.The aim of this study was to investigate the effects of PA2146 on the virulence of P.aeruginosa and explore its mechanism.Methods:PA2146 gene knockout strain and complement strain of P.aeruginosa PAO1 were constructed.The biomass of biofilm was detected by crystal violet staining;the virulence factors were measured,including pyocyanin,rhamnolipid,LasA elastase,LasB elastase and hemolytic activity;RNA-seq and label-free relative quantitative proteomics analyses were carried out to test the influence of PA2146 on transcriptomics and proteomics.This study was approved by the Institutional Review Board of the Third Xiangya Hospital,Central South University,China(approval No.2019-S021).Results:PA2146-deficient strains showed reduced biofilm formation and increased pyocyanin,rhamnolipid,LasA elastase,LasB elastase,and hemolytic activity,as well as increased motility,compared with the wild-type strain.RNA-seq and label-free relative quantitative proteomics analyses revealed that PA2146 repressed the transcription of several genes that are integral to the pqs system and to pyocyanin biosynthesis,and increased the expression of MexEF-OprN efflux pump components at the gene and protein level.Conclusion:PA2146 gene was found to inhibit the pqs system.PA2146 may affect quorum sensing by directly inhibiting the pqs system or by enhancing the expression of MexEF-OprN efflux pump components,thereby promoting efflux of 2-heptyl-4(1H)-quinolone,a Pseudomonas quinolone signal precursor,and thus affecting P.aeruginosa virulence.

关键词： bacterial virulence PA2146 Pseudomonas aeruginosa QS quorum sensing

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