Bispecific aptamer-initiated 3D DNA nanomotor biosensor powered by DNAzyme and entropy-driven circuit for sensitive and specificity detection of lysozyme
作者机构：State Key Laboratory of Food Science and TechnologyInternational Joint Laboratory on Food SafetyJiangnan UniversityWuxi 214122China School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Key Laboratory of Meat Processing of SichuanChengdu UniversityChengdu 610106China National Engineering Research Center for Functional FoodJiangnan UniversityWuxi 214122China Collaborative Inno vation Center of Food Safety and Quality Control in Jiangsu ProvinceJiangnan UniversityWuxi 214122China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2023年第16卷第1期
页 面：1286-1295页
核心收录：
学科分类：08[工学] 080202[工学-机械电子工程] 0805[工学-材料科学与工程（可授工学、理学学位）] 0802[工学-机械工程] 0703[理学-化学] 0702[理学-物理学]
基 金：This work was partly funded by the National Natural Science Foundation of China(Nos.31871881 and 31871721) the National First-class Discipline Program of Food Science and Technology(No.JUFSTR20180303) the National High-Level Personnel of Special Support Program(No.W03020371).
主 题：bispecific aptamer DNA rolling DNA walking amplification specificity
摘 要：Dynamic DNA nanodevices have gained tremendous attention due to their extraordinary inherent functionality and advantages,however,dynamic DNA nanodevices-based biosensors are still challenging due to their high reliance on proteases and limited amplification capabilities.Herein,exploiting bispecific aptamer as initiators for the first time,we developed a three-dimensional(3D)DNA nanomotor biosensor powered by DNAzyme and entropy-driven circuit for sensitive and specific detection of lysozyme,in which walking and rolling strategies are efficiently integrated to achieve excellent signal amplification capability.Benefiting from the high selectivity of bispecific aptamer,the 3D DNA nanomotor biosensor can respond to lysozyme with high specificity and operate at high speed to release signals.The whole process is independent of protease,avoiding the influence of adverse environment on the operation stability.Under optimal conditions,it can achieve a limit of detection as low as 0.01 pg/mL with an excellent linear range of 0.05 pg/mL–500 ng/mL for lysozyme.Furthermore,the proposed strategy revealed high accuracy in the analysis of real samples,indicating a great potential for the application of nanomotor biosensors to the detection of non-nucleic acid targets.
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