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Frontiers of physics 2023年第5期18卷 183-192页

作者： Yue Ding Chonghui Li Meng Tian Jihua Wang Zhenxing Wang Xiaohui Lin Guofeng Liu Wanling Cui xuefan Qi Siyu Li Weiwei Yue shicai xu School of Physics and Electronics Shandong Normal UniversityJinan 250014China Shandong Key Laboratory of Biophysics Institute of BiophysicsDezhou UniversityDezhou 253023China Shandong Engineering Laboratory of Swine Herd Health Big Data and Intelligent Monitoring Dezhou UniversityDezhou 253023China

Adenosine triphosphate(ATP)is closely related to the pathogenesis of certain diseases,so the detection of trace ATP is of great significance to disease diagnosis and drug development.Graphene field-effect transistors(GFETs)have been proven to be a promising platform for the rapid and accurate detection of small molecules,while the Debye shielding limits the sensitive detection in real samples.Here,a three-dimensional wrinkled graphene field-effect transistor(3D WG-FET)biosensor for ultra-sensitive detection of ATP is demonstrated.The lowest detection limit of 3D WG-FET for analyzing ATP is down to 3.01 aM,which is much lower than the reported results.In addition,the 3D WG-FET biosensor shows a good linear electrical response to ATP concentrations in a broad range of detection from 10 aM to 10 pM.Meanwhile,we achieved ultra-sensitive(LOD:10 aM)and quantitative(range from 10 aM to 100 fM)measurements of ATP in human serum.The 3D WG-FET also exhibits high specificity.This work may provide a novel approach to improve the sensitivity for the detection of ATP in complex biological matrix,showing a broad application value for early clinical diagnosis and food health monitoring.

关键词： ATP in complex human serum three-dimensional wrinkled graphene field effect transistor Debye shielding ultra-sensitive

Mechanical stretching boosts expansion and regeneration of intestinal organoids through fueling stem cell self-renewal

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Cell Regeneration 2022年第1期11卷 399-412页

作者： Fanlu Meng Congcong Shen Li Yang Chao Ni Jianyong Huang Kaijun Lin Zanxia Cao shicai xu Wanling Cui Xiaoxin Wang Bailing Zhou Chunyang Xiong Jihua Wang Bing Zhao Shandong Key Laboratory of Biophysics Institute of BiophysicsDezhou UniversityDezhou 253023China Department of Mechanics and Engineering Science College of EngineeringPeking UniversityBeijing 100871China State Key Laboratory of Genetic Engineering School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Beijing Innovation Center for Engineering Science and Advanced Technology College of EngineeringPeking UniversityBeijing 100871China Wenzhou Institute University of Chinese Academy of SciencesOujiang LaboratoryWenzhou 325000ZhejiangChina

Intestinal organoids,derived from intestinal stem cell self-organization,recapitulate the tissue structures and behav-iors of the intestinal epithelium,which hold great potential for the study of developmental biology,disease modeling,and regenerative medicine.The intestinal epithelium is exposed to dynamic mechanical forces which exert profound effects on gut development.However,the conventional intestinal organoid culture system neglects the key role of mechanical microenvironments but relies solely on biological factors.Here,we show that adding cyclic stretch to intestinal organoid cultures remarkably up-regulates the signature gene expression and proliferation of intestinal stem cells.Furthermore,mechanical stretching stimulates the expansion of SOX9+progenitors by activating the Wnt/β-Catenin signaling.These data demonstrate that the incorporation of mechanical stretch boosts the stemness of intestinal stem cells,thus benefiting organoid growth.Our findings have provided a way to optimize an organoid generation system through understanding cross-talk between biological and mechanical factors,paving the way for the application of mechanical forces in organoid-based models.

关键词： Mechanical stretching Intestinal organoid Lgr5+stem cell Regeneration Wnt/β-catenin signaling

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关于第二类Zagreb指数猜想的一个证明

中国科学：数学 2024年第1期54卷 105-120页

作者：龚世才罗高炫浙江科技学院理学院杭州310023

设G是一个n阶简单图.G的第二类Zagreb指数定义为M_(2)(G)=∑ij∈E(G)d_(i)d_(j).其中d_(i)表示顶点i的度.xu等(2014)提出了一个关于第二类Zagreb指数的猜想:在所有阶数为n、边数为m的图中,M_(2)(G)最大的图是拟完全的.借助于门槛图的Fer... 详细信息

设G是一个n阶简单图.G的第二类Zagreb指数定义为M_(2)(G)=∑ij∈E(G)d_(i)d_(j).其中d_(i)表示顶点i的度.xu等(2014)提出了一个关于第二类Zagreb指数的猜想:在所有阶数为n、边数为m的图中,M_(2)(G)最大的图是拟完全的.借助于门槛图的Ferrers表的性质,本文将上述猜想转化为组合矩阵论优化问题,并给出该猜想的一个代数证明.

关键词： Zagreb指数 Ferrers表门槛图度

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脊柱椎间盘退变与高尿酸血症的相关性

中国组织工程研究 2024年第32期28卷 5091-5096页

作者：李洋马飞冷叶波徐世财何宝强周佳俊廖烨晖唐强唐超王清钟德君西南医科大学附属医院骨科四川省泸州市646000 西南医科大学附属天府医院骨科四川省眉山市620036 陆军军医大学新桥医院骨科重庆市400030

背景:高尿酸血症是常见的代谢性疾病,高尿酸血症患者以尿酸结晶形成导致痛风为主要临床表现。既往研究仅报道了尿酸结晶会导致脊柱椎间盘的退变,但关于高尿酸血症与脊柱椎间盘退变的相关性研究较少。目的:回顾性分析高尿酸血症患者脊柱... 详细信息

背景:高尿酸血症是常见的代谢性疾病,高尿酸血症患者以尿酸结晶形成导致痛风为主要临床表现。既往研究仅报道了尿酸结晶会导致脊柱椎间盘的退变,但关于高尿酸血症与脊柱椎间盘退变的相关性研究较少。目的:回顾性分析高尿酸血症患者脊柱椎间盘的退变特点以及血尿酸浓度与脊柱椎间盘退变的相关性。方法:回顾性分析2021年1月至2022年12月在西南医科大学附属医院骨科就诊并被诊断为脊柱椎间盘退变的所有患者,纳入97例高尿酸血症患者作为高尿酸血症组,然后根据性别、年龄按照1∶2进行匹配,将194例非高尿酸血症患者作为对照组。收集两组患者的血尿酸检验结果,并在全脊柱MRI图像上对两组患者的椎间盘退变程度进行Pfirrmann评分。比较两组患者椎间盘退变程度的差异,分析血尿酸浓度与椎间盘退变程度的相关性。结果与结论:①高尿酸血症组的椎间盘退变程度Pfirrmann评分大于对照组,且高尿酸血症组的椎间盘退变总数大于对照组,差异均有显著性意义(P0.05);④结果提示:在高尿酸血症患者中,血尿酸浓度越高,椎间盘退变程度越严重。因此,高尿酸血症是导致椎间盘退变的危险因素之一。

关键词：高尿酸血症椎间盘退变 Pfirrmann评分脊柱MRI

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基于FPGA的光纤陀螺本征频率自动测量

智能计算机与应用 2024年第5期14卷 126-129页

作者：高辉马家君徐榆鑫白仕才徐文正孟鋆贵州大学大数据与信息工程学院贵阳550025

为实现光纤陀螺本征频率的高精度自动测量,本文基于FPGA提出了一种本征频率自动测量算法。理论研究了方波调制信号与光强信号之间的关系,获得了光纤陀螺本征频率测量误差信号,根据自动控制原理,通过闭环测量系统将本征频率测量误差信号... 详细信息

为实现光纤陀螺本征频率的高精度自动测量,本文基于FPGA提出了一种本征频率自动测量算法。理论研究了方波调制信号与光强信号之间的关系,获得了光纤陀螺本征频率测量误差信号,根据自动控制原理,通过闭环测量系统将本征频率测量误差信号积分进而实时跟踪光纤陀螺本征频率,当测量误差信号为0,方波调制频率即为光纤陀螺本征频率。实验结果表明,该算法可以实现光纤陀螺本征频率的高精度自动测量。与其他算法相比,本文提出的算法测量精度达到1 Hz,具有高精度、速度快、易实现等优点。

关键词：光纤光学光纤陀螺频率跟踪本征频率

全脊柱MRI评估骨质疏松患者椎体骨折的分布特点及临床意义

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中国组织工程研究 2025年第9期29卷 1883-1889页

作者：周佳俊马飞冷叶波徐世财何宝强李洋廖烨晖唐强唐超王清钟德君西南医科大学附属医院骨科四川省泸州市646000 陆军军医大学新桥医院骨科重庆市400030

背景:骨质疏松性椎体骨折是骨质疏松患者最常见的并发症。脊柱MRI作为一种新的成像技术,在诊断骨质疏松性椎体骨折方面的灵敏度远高于X射线平片。但全脊柱MRI费用高,扫描时间长。因此,对于骨质疏松性椎体骨折患者,是否都需要进行全脊柱... 详细信息

背景:骨质疏松性椎体骨折是骨质疏松患者最常见的并发症。脊柱MRI作为一种新的成像技术,在诊断骨质疏松性椎体骨折方面的灵敏度远高于X射线平片。但全脊柱MRI费用高,扫描时间长。因此,对于骨质疏松性椎体骨折患者,是否都需要进行全脊柱MRI扫描以及哪些患者需要行全脊柱MRI,目前临床上尚无统一定论。目的:通过观察骨质疏松性椎体骨折患者的全脊柱MRI影像学资料,分析椎体骨折的分布特点,并探讨其临床意义。方法:回顾性分析2018年8月至2022年9月在西南医科大学附属医院骨科就诊并被诊断为骨质疏松性椎体骨折患者的病历资料和MRI图像,根据纳入和排除标准将903例患者纳入研究。收集患者一般资料(年龄、性别、体质量指数)、病史特点(病程时间、脊柱外伤手术史、叩击痛区域、疼痛评分等);通过全脊柱MRI图像分析其椎体骨折特点。首先根据患者椎体骨折数量将患者分为单椎体骨折组(n=484)和多椎体骨折组(n=419),并分析两组间的差异。然后再根据骨折椎体间的最远间隔数是否≥5将多椎体骨折组再分为2个亚组,其中A组(骨折椎体最远间隔数0.05);③A组与B组进行比较,两组患者骨密度、叩击痛区域分布及数量、低能量损伤病史相比差异有显著性意义(P0.05);④提示对于有多次低能量创伤史、病史时间大于1个月伴多重叩诊痛、骨密度较低的患者,应警惕多发椎体骨折、跳跃骨折的发生,建议这些患者进行全脊柱MRI检查。

关键词：骨质疏松椎体骨折全脊柱MRI 骨折特点骨密度多椎体骨折

Predicted methylation landscape of all CpG islands on the human genome

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Chinese Science Bulletin 2010年第22期55卷 2353-2358页

作者： FAN shicai ZOU JianXiao xu HongBing ZHANG xueGong School of Automation Engineering University of Electronic Science and Technology of China Chengdu 610054 China MOE Key Laboratory of Bioinformatics and Bioinformatics Division TNLIST/ Department of Automation Tsinghua University Beijing 100084 China

CpG island methylation plays important role in various biological processes. To investigate methylation landscape of all CpG islands on the human genome, we develop a model for predicting the CpG island methylation status. This model outperforms other existing methods. We apply the model on the whole human genome and predict the landscape of DNA methylation of all CpG islands. Based on the methylation profile, we find that about 31% of CpG islands are methylation-prone and CpG islands located in promoter regions are seldom methylated. There is no significant difference in the CpG island methylation level between R and G bands among the chromosomes. The occupancy of RNA polymerase II is significantly higher in methylation-resistant promoter CpG islands, indicating that genes with such promoter CpG islands tend to be more active.

关键词： DNA甲基化人类基因组 CpG岛景观预测 RNA聚合酶基因启动子生物过程

不同收获期对杂交水稻F_(1)代种子质量的影响

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浙江农业科学 2024年第4期65卷 808-811页

作者：顾舒雯徐鸿诚李哲陈锦珠吴海峰杨世才潘竹栋朱孔志江苏金色农业股份有限公司江苏大丰224100

为探究杂交水稻F_(1)代不同收获期对种子质量的影响,选择3个杂交水稻制种组合,分别是晶4155S/R534、荃9311A/五山丝苗和隆臻36S/钰占作为试验材料,在喷施赤霉素后设置8个不同收获期,研究不同收获时间对杂交水稻F_(1)代种子质量的影响,... 详细信息

为探究杂交水稻F_(1)代不同收获期对种子质量的影响,选择3个杂交水稻制种组合,分别是晶4155S/R534、荃9311A/五山丝苗和隆臻36S/钰占作为试验材料,在喷施赤霉素后设置8个不同收获期,研究不同收获时间对杂交水稻F_(1)代种子质量的影响,考察相关农艺性状。结果表明,晶4155S/R534在喷施赤霉素后33 d左右种子综合质量较好,适宜收获;荃9311A/五山丝苗,在喷施赤霉素后30 d左右种子综合质量较好,适宜收获;而隆臻36S/钰占在喷施九二〇后35 d左右适宜收获。因此,选择适宜的收割时期可显著提高杂交水稻种子发芽率和发芽势,增加千粒重,降低种子粉质化程度和裂颖率,提升杂交水稻种子质量,确保杂交水稻产量稳收。

关键词：杂交水稻赤霉素收获期种子质量

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Spatial and Temporal Distribution of Au and Pb-Zn Mineralization:Phenomenon,Mechanism and Implication

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Acta Geologica Sinica(English Edition) 2000年第3期74卷 504-510页

作者： WANG Dongbo SHAO shicai xu Yong LIU Guoping Beijing Institute of Geology for Mineral Resources Beijing 100012 State Nonferrous Metals Industry Administration Beijing 100814

In the 1990s, some median-large gold deposits have been discovered in several lead-zinc metallogenetic belts (e.g. the Qinling lead-zinc metallogenetic belt, Shanxi Province and Gansu Province and the Qingchengzi lead-zinc ore field, Liaoning Province) in China. Gold deposits and lead-zinc deposits spatially co-exist in the same tectonic setting; lead-zinc orebodies are commonly located below gold ore bodies. The host rocks of lead-zinc ore-bodies are conformably overlain by those of gold ore bodies. The age of gold mineralization is obviously younger than that of lead-zinc mineralization. Preliminary geochemical research has demonstrated the following: lead-zinc mineralization took place in a marine sedimentary-exhalative system, which had the characteristics of a high fluid/rock ratio, a high salinity and a high halide activity; meanwhile, most of gold was transported into the low-temperature hydrothermal plume and primarily enriched in sediments. During later (magmatism-) metamorphism- tectonism, gold was remobilized and transported into the metamorphic fluid which had the characteristics of medium- high temperatures, a low fluid/rock ratio and a low activity of halide, and precipitated at a favourable structural site. Therefore, the co-existence of gold and lead-zinc deposits and the separation of gold from lead-zinc result from the differences of chemical composition and circulation of ore fluids in the same tectonic unit. This phenomenon can be used as an important criterion in exploration.

关键词： gold deposit lead-zinc deposit spatial-temporal distribution ore fluid

Relationship Between Allelopathic Effects and Functional Traits of Different Allelopathic Potential Rice Accessions at Different Growth Stages

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Rice science 2018年第1期25卷 32-41页

作者： xu Gaofeng SHEN shicai ZHANG Fudou ZHANG Yun Kato-Noguchi HISASHI Roy Clements DAVID Institute of Agricultural Environment and Resources Research Yunnan Academy of Agricultural Sciences Food Crops Research Institute Yunnan Academy of Agricultural Sciences Department of Applied Science Faculty of Agriculture Kagawa University Department of Biology Trinity Western University

In this study,effects of temperature,light and their interactions on allelopathic effects and the functional traits specific leaf area(SLA)and stem mass fraction(SMF)of different allelopathic potential rice accessions at different growth stages were analyzed.The main results were as follows:Allelopathic responses to temperature and light varied with different allelopathic potential rice accessions at different growth stages.With the rise of temperature and the extension of photoperiod,allelopathic effect increased firstly and then decreased at 2–3 leaf stage,but increased constantly at the 4–5 and 7–8 leaf stages in strong allelopathic rice accessions[O.longistaminata,F1(O.longistaminata×RD23),F2(RL159 and RL169)].Temperature had significant impact on allelopathic effect without considering light factors,but light showed little effect on rice allelopathy at the same temperature conditions.The greatest allelopathic effect was attained with moderate temperature and long photoperiod at 2–3 leaf stage in strong allelopathic rice accessions,but all the rice accessions showed weak allelopathic effects at the low temperature condition(15oC/10oC),and the influence of different factors on allelopathy followed a general trend as temperature>leaf stage>light,indicating that among the multiple factors impacting rice allelopathy,temperature was the main factor.Allelopathic characteristics of F1 and F2 to various temperature and light were similar to O.longistaminata,showing that allelopathic genes from wild rice can be expressed in its descendants.Temperature and light also had significant effects on SLA and SMF,and rice allelopathy was closely correlative to SLA in strong allelopathic rice accessions at the 4–5 and 7–8 leaf stages,but there was no correlation between rice allelopathy and SMF at different growth stages.These results suggested that rice adjust the relationship between allelopathy and SLA and adapt to the varied environments,and that high temperature and long photoperiod can enhance rice allelopathic activity.

关键词： rice allelopathy environment change functional trait specific leaf area stem mass fraction temperature-light interaction