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Significance of hepatitis B virus surface antigen, hepatitis C virus expression in hepatocellular carcinoma and pericarcinomatous tissues

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World Journal of Gastroenterology 2007年第12期13卷 1870-1874页

作者： shi-ying xuan Yong-Ning Xin Hua Chen Guang-Jun shi Hua-shi Guan Yang Li College of Medicine and Pharmaceutics Ocean University of China 5 Yushan Road Qingdao 266003 Shandong Province China Qingdao Municipal Hospital Qingdao University Medical College Qingdao 266021 Shandong Province China

AIM： To investigate the correlation between hepatitis B virus surface antigen （HBsAg）, hepatitis C virus （HCV） expression in hepatocellular carcinoma （HCC）, the HAI score of the noncancerous region of the liver and the serum Alpha fetoprotein （AFP） level. METHODS： The patterns of HBsAg and HCV in 100 cases of HCC and their surrounding liver tissues were studied on paraffin-embedded sections with immunohistochemistry, the histological status was determined by one pathologist and one surgeon simultaneously using the hepatitis activity index （HAIl score, and AFP was detected by radioimmunity. The study included 100 consecutive patients who underwent curative resection for HCC. Based on HBsAg and HCV expression, the patients were classified into 4 groups： patients positive for HBsAg （HBsAg group）, patients positive for HCV （HCV group）, patients negative for both HCV and HBsAg （NBNC group） and patients positive for both HBsAg and HCV （BC group）. RESULTS： The BC group had significantly higher HAI scores than the other three groups. （BC 〉 HCV 〉 HBsAg 〉 NBNC）. HBV and HCV virus infection was positively correlated with HAI （rs = 0.39, P = 0.00011. The positive rate of AFP （85.7%） and the value of AFP （541.2 ng/mL） in the group with HBV and HCV co-infection were the highest among the four groups. The positive rate （53.3%） of AFP and the value of AFP （ 53.3 ng/mL） in the group with none-infection of HBV and HCV were the lowest. HBV and HCV virus infection was positively correlated with AFP（rs = 0.38, P = 0.0001）. CONCLUSION： The AFP increase in patients with liver cancer was positively correlated with the infection of HBV and HCV. The-serum AFP elevation by the infection of HBV and HCV is one of mechanisms which lead to hepatocarcinogenesis, and the antivirus intervening treatment of hepatitis is significant for the prognosis of liver cancer. From our Spearman＇s rank correlation analysis, we can conclude that the severity of virally induced inflammation is correlated wit

关键词： Hepatitis B virus surface antigen Hepatitis C virus antigen Histological activity index Immunohistochemistry Hepatocellular carcinoma Alpha-fetoprotein.

Helicobacter infection in hepatocellular carcinoma tissue

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World Journal of Gastroenterology 2006年第15期12卷 2335-2340页

作者： shi-ying xuan Ning Li Xin Qiang Rong-Rong Zhou Yong-Xin shi Wen-Jie Jiang Qingdao Municipal Hospital Affiliated to School of Medicine Qingdao UniversityQingdao 266021 Shandong Province China Department of Microbiology School of Medicine Qingdao University Qingdao266021 Shandong Province China

AIM： To investigate whether Helicobacter species （Helicobacter spp.） could be detected in hepatocellular carcinoma （HCC） tissue.METHODS： Liver samples from 28 patients with hepatocellular carcinoma （HCC） diagnosed by histopathology were studied. Twenty-two patients with other liver diseases （5 with liver trauma, 7 with cavernous liver hemangioma, 6 with liver cyst and 4 with hepatolithiasis）, 25 patients with gastric cancer, 15 with colonic cancer and 15 with myoma of uterus served as controls. Two piceces of biopsy were obtained from each patient. One was cultured for Helicobacter spp. and extraction of DNA, the other was prepared for scanning electron microscopy （SEM） and in situ hybridization. The samples were cultured on Columbia agar plates with microaerobic techniques. Helicobacter spp. in biopsy from the studied subjects was detected by polymerase chain reaction （PCR） with Helicobacter spp. 16S rRNA primers. Amplified products were identified by Southern hybridization and sequenced further. Besides, other genes （vacA, cagA） specific for Helicobacter pylori （H pylorO were also detected by PCR. Helicobacter spp. in biopsies was observed by SEM. Transmission electron microscopy （TEM） was performed to identify the cultured positive Helicobacter spp. The presence of Helicobacter spp. was detected by in situ hybridization to confirm the type of Helicobacter. RESULTS： The positive rate of He/icobacter cultured in HCC and gastric cancer tissue was 10.7% （3/28） and 24%（6/25）, respectively. Helicobacter microorganisms were identified further by typical appearance on Gram staining, positive urease test and characteristic colony morphology on TEM. The bacterium was observed in adjacent hepatocytes of the two HCC samples by *** number of cocci was greater than that of bacilli. The bacterium was also found in four gastric cancer samples. PCR showed that the positive rate of HCC and gastric cancer samples was 60.7% and 72% respectively, while the controls were negat

关键词： Hepatocellular carcinoma Helicobacter infection

Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC

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Journal of Clinical and Translational Hepatology 2022年第5期10卷 901-912页

作者： Zhong-Hua Lin Jie Zhang Li-Kun Zhuang Yong-Ning Xin shi-ying xuan College of Medicine and Pharmaceutics Ocean University of ChinaQingdaoShandongChina Department of Gastroenterology Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Medical College Qingdao UniversityQingdaoShandongChina Clinical Research Center Qingdao Municipal HospitalQingdao UniversityQingdaoShandongChina

Background and Aims:Generally acceptable prognostic models for hepatocellular carcinoma(HCC)are not *** study aimed to establish a prognostic model for HCC by identifying immune-related differentially expressed genes(IR-DEGs)and to investigate the potential role of NR6A1 in the progression of ***:Bioinformatics analysis using The Cancer Genome Atlas and ImmPort databases was used to identify *** Cox regression and multivariate Cox regression analysis were used to establish a prognostic model of ***-Meier analysis and the receiver operating characteristic(ROC)curves were used to evaluate the performance of the prognostic model,which was further verified in the International Cancer Genome Consortium(ICGC)*** set enrichment analysis was used to explore the potential pathways of *** counting kit 8,colony formation,wound healing,and Transwell migration assays using Huh7 cells,and tumor formation models in nude mice were ***:A prognostic model established based on ten identified IR-DEGs including HSPA4,FABP6,MAPT,NDRG1,APLN,IL17D,LHB,SPP1,GLP1R,and NR6A1,effectively predicted the prognosis of HCC patients,was confirmed by the ROC curves and verified in ICGC ***6A1 expression was significantly up-regulated in HCC patients,and NR6A1 was significantly associated with a low survival *** set enrichment analysis showed the enrichment of cell cycle,mTOR,WNT,and ERBB signaling pathways in patients with high NR6A1 ***6A1 promoted cell proliferation,invasiveness,migration,and malignant tumor formation and growth in vitro and in ***:An effective prognostic model for HCC,based on a novel signature of 10 immune-related genes,was ***6A1 was up-regulated in HCC and was associated with a poor prognosis of ***6A1 promoted cell proliferation,migration,and growth of HCC,most likely through the cell cycle,mTOR,WNT,and ERBB signaling pathways.

关键词： Hepatocellular carcinoma Bioinformatics analysis Prognostic model NR6A1 Proliferation Migration Tumorigenesis

Association between the presence of H pylori in the liver and hepatocellular carcinoma: A meta-analysis

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World Journal of Gastroenterology 2008年第2期14卷 307-312页

作者： shi-ying xuan Yong-Ning Xin An-Jin Chen Quan-Jiang Dong Xin Qiang Ning Li Ming-Hua Zheng Hua-shi Guan College of Medicine and Pharmaceutics Ocean University of China Qingdao 266003Shandong Province China Qiaodao Municipal Hospital Qingdao University Medical College Qingdao 266021 Shandong Province China Department of Microbiology School of MedicineQingdao University Qingdao 266021 Shandong Province China Department of Infection and Liver Disease The First Affiliated Hospital of Wenzhou Medical CollegeWenzhou 325000 Zhejiang Province China

AIM： To evaluate the arguments for and against the possible roles of H pylori in hepatocellular carcinoma （HCC）. METHODS： We performed a systematic review of all relevant studies published in the literature. A total of 103 clinical trials and reports were identified, but only 10 trials qualified under our selection criteria. A metaanalysis was carried out by a biostatistician according to the Cochrane Reviewers＇ Handbook recommended by The Cochrane Collaboration. RESULTS： Nine case-control studies and one retrospective cross sectional study were included in the final analysis. Overall the prevalence of H pylori infection was 53.3% （129 of 242） in cases and 10.4% （29 of 280） in controls, and the summary odds ratio for the association of H pylori infection with the risk for HCC （using the fixed-effects model, which accounted for the homogeneity across the 10 studies） was determined to be 13.63 （95% CI, 7.90-23.49）. CONCLUSION： Our analysis showed a positive association between F1 pylori infection and the risk of HCC, with an indication of possible publication bias and possible confounders due to study designs that showed results of less pronounced associations.

关键词： H pylori Hepatocellular carcinoma Meta-analysis Publication bias

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World Journal of Gastroenterology 2012年第45期18卷 6571-6576页

作者： Quan-Jiang Dong Shu-Hui Zhan Li-Li Wang Yong-Ning Xin Man Jiang shi-ying xuan Central Laboratories Department of GastroenterologyQingdao Municipal Hospital

Helicobacter pylori （H. pylorl） is a Gram-negative bac- terium that infects half of the human population. The infection is associated with chronic inflammation of the gastric mucosa and peptic ulcers. It is also a major risk factor for gastric cancer. Phylogenetic analysis of global strains reveals there are seven populations of H. pylori, including hpAfrical, hpAfrica2, hpEastAsia, hpEurope, hpNEAfrica, hpAsia2 and hpSahul. These populations are consistent with their geographical origins, and pos- sibly result from geographical separation of the bac- terium leading to reduced bacterial recombination in some populations. For each population, H. pylori has evolved to possess genomic contents distinguishable from others. The hpEurope population is distinct in that it has the largest genome of 1.65 mbp on average, and the highest number of coding sequences. This confers its competitive advantage over other populations but at the cost of a lower infection rate. The large genomic size could be a cause of the frequent occurrence of the deletion of the cag pathogenicity island in H. pylori strains from hpEurope. The incidence of gastric cancer varies among different geographical regions. This can be attributed in part to different rates of infection of H. pylori. Recent studies found that different popula- tions of H, pylori vary in their carcinogenic potential and contribute to the variation in incidence of gastric cancer among geographical regions. This could be related to the ancestral origin of H, pylori. Further studies are indi- cated to investigate the bacterial factors contributing to differential virulence and their influence on the clinical features in infected individuals.

关键词： Helicobacter pylori Population genetics Gastric cancer Virulence Genome

PNPLA3 I148M variant in nonalcoholic fatty liver disease:Demographic and ethnic characteristics and the role of the variant in nonalcoholic fatty liver fibrosis

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World Journal of Gastroenterology 2015年第3期21卷 794-802页

作者： Li-Zhen Chen Yong-Ning Xin Ning Geng Man Jiang Ding-Ding Zhang shi-ying xuan Department of Gastroenterology Qingdao Municipal Hospital Medical College of Qingdao University

Patatin-like phospholipase domain-containing 3(PNPLA3 or adiponutrin) displays anabolic and catabolic activities in lipid metabolism,and has been reported to be significantly associated with liver fat *** have established a strong link between the 148 isoleucine to methionine protein variant(I148M) of PNPLA3 and liver diseases,including nonalcoholic fatty liver disease(NAFLD).However,detailed demographic and ethnic characteristics of the I148 M variant and its role in the development of nonalcoholic fatty liver fibrosis have not been fully *** present review summarizes the current knowledge on the association between the PNPLA3 I148 M variant and NAFLD,and especially its role in the development of nonalcoholic fatty liver ***,we analyze the impact of demographic and ethnic characteristics of the PNPLA3 I148 M variant and the presence of metabolic syndrome on the association between PNPLA3 I148 M and ***,we explore the role of the PNPLA3 I148 M in the development of nonalcoholic fatty liver fibrosis,and hypothesize the underlying mechanisms by speculating a pro-fibrogenic ***,we briefly highlight future research that may elucidate the specific mechanisms of the PNPLA3 I148 M variant in fibrogenesis,which,in turn,provides a theoretical foundation and valuable experimental data for the clinical management of nonalcoholic fatty liver fibrosis.

关键词： PNPLA3 I148M variant Polymorphism Nonalcoholic fat

Lack of association between apolipoprotein C3 gene polymorphisms and risk of nonalcoholic fatty liver disease in a Chinese Han population

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World Journal of Gastroenterology 2014年第13期20卷 ing3655-3662页

作者： Tong-Hong Niu Man Jiang Yong-Ning Xin Xiang-Jun Jiang Zhong-Hua Lin shi-ying xuan Medical College of Qingdao University Department of Gastroenterology Qingdao Municipal Hospital

AIM: To investigate the association between two polymorphisms of apolipoprotein C3 (APOC3) and risk of nonalcoholic fatty liver disease (NAFLD) in a Chinese Han population.

关键词： Polymorphism Single nucleotide Nonalcoholic fatty liver disease Apolipoprotein C3 Insulin resistance Oxidative stress

Specific HLA-DQB1 alleles associated with risk for development of hepatocellular carcinoma:A meta-analysis

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World Journal of Gastroenterology 2011年第17期17卷 2248-2254页

作者： Yong-Ning Xin Zhong-Hua Lin Xiang-Jun Jiang Shu-Hui Zhan Quan-Jiang Dong Qing Wang shi-ying xuan Department of Gastroenterology Qingdao Municipal Hospital Qingdao 266071 Shandong Province China School of Medicine Qingdao University Qingdao 266021 Shandong Province China College of Medicine and Pharmaceutics Ocean University of China Qingdao 266003 Shandong Province China

AIM:To evaluate the association of human leukocyte antigen(HLA)-DQB1 alleles with hepatocellular carcinoma(HCC) through meta-analysis of published ***:Case-control studies on HLA-DQB1 allele association with HCC published up to January 2010 were included in the *** odds ratios(ORs) of HLADQB1 allele distributions in HCC patients were analyzed and compared with healthy *** meta-analysis software REVMAN 5.0 was applied for investigating heterogeneity among individual studies and for summarizing all the studies.A meta-analysis was performed using fixed-effect or random-effect methods,depending on the absence or presence of significant *** case-control studies containing 398 cases and 594 controls were included in the final ***:Among the five family alleles,two(DQB1*02 and DQB1*03) were found to be significantly associated with the risk of *** combined OR for the association of DQB1*02 and DQB1*03 allele with the risk for HCC was 1.78(95% CI:1.05-3.03,P = 0.03) and 0.65(95% CI:0.48-0.89,P = 0.007),*** the 13 specific alleles,two(DQB1*0502 and DQB1*0602) were significantly associated with risk of *** combined OR for the association of DQB1*0502 and DQB1*0602 allele with the risk for HCC was 1.82(95% CI:1.14-2.92,P = 0.01) and 0.58(95% CI:0.36-0.95,P = 0.03),*** significant association was established for other HLA-DQB1 family alleles and specific ***:Our results support the hypothesis that specific HLA-DQB1 allele families and alleles might influence the susceptibility or resistance to HCC,although it needs further investigations.

关键词： Hepatocellular carcinoma Human leukocyte antigen-DQB1 alleles Meta-analysis

Reduced genome size of Helicobacter pylori originating from East Asia

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World Journal of Gastroenterology 2014年第19期20卷 5666-5671页

作者： Quan-Jiang Dong Li-Li Wang Zi-Bing Tian Xin-Jun Yu Sheng-Jiao Jia shi-ying xuan Central Laboratories and Department of Gastroenterology Qingdao Municipal Hospital Department of Gastroenterology the Affiliated Hospital of Qingdao University

Helicobacter pylori(***),a major pathogen colonizing the human stomach,shows great genetic *** analysis of strains from different *** populations revealed that the genome size of strains from East Asia decreased to 1.60 Mbp,which is significantly smaller than that from Europe or *** parallel with the genome reduction,the number of protein coding genes was decreased,and the guaninecytosine content was lowered to 38.9%.Elimination of non-essential genes by mutations is likely to be a major cause of the genome *** with a small genome cost less ***,*** strains from East Asia may have proliferation and growth advantages over those from Western *** could result in enhanced capacity of bacterial ***,the reduced genome size potentially contributes to the high prevalence of *** in East Asia.

关键词： Helicobacter pylori Genome Mutation Epidemiology Recombination