Establishment of a Prognostic Model for Hepatocellular Carcinoma Based on Bioinformatics and the Role of NR6A1 in the Progression of HCC

作     者：Zhong-Hua Lin Jie Zhang Li-Kun Zhuang Yong-Ning Xin Shi-Ying Xuan 

作者机构：College of Medicine and PharmaceuticsOcean University of ChinaQingdaoShandongChina Department of GastroenterologyQingdao Municipal HospitalQingdao UniversityQingdaoShandongChina Medical CollegeQingdao UniversityQingdaoShandongChina Clinical Research CenterQingdao Municipal HospitalQingdao UniversityQingdaoShandongChina 

出 版 物：《Journal of Clinical and Translational Hepatology》 (临床与转化肝病杂志（英文版）)

年 卷 期：2022年第10卷第5期

页      面：901-912页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Hepatocellular carcinoma Bioinformatics analysis Prognostic model NR6A1 Proliferation Migration Tumorigenesis 

摘      要：Background and Aims:Generally acceptable prognostic models for hepatocellular carcinoma(HCC)are not *** study aimed to establish a prognostic model for HCC by identifying immune-related differentially expressed genes(IR-DEGs)and to investigate the potential role of NR6A1 in the progression of ***:Bioinformatics analysis using The Cancer Genome Atlas and ImmPort databases was used to identify *** Cox regression and multivariate Cox regression analysis were used to establish a prognostic model of ***-Meier analysis and the receiver operating characteristic(ROC)curves were used to evaluate the performance of the prognostic model,which was further verified in the International Cancer Genome Consortium(ICGC)*** set enrichment analysis was used to explore the potential pathways of *** counting kit 8,colony formation,wound healing,and Transwell migration assays using Huh7 cells,and tumor formation models in nude mice were ***:A prognostic model established based on ten identified IR-DEGs including HSPA4,FABP6,MAPT,NDRG1,APLN,IL17D,LHB,SPP1,GLP1R,and NR6A1,effectively predicted the prognosis of HCC patients,was confirmed by the ROC curves and verified in ICGC ***6A1 expression was significantly up-regulated in HCC patients,and NR6A1 was significantly associated with a low survival *** set enrichment analysis showed the enrichment of cell cycle,mTOR,WNT,and ERBB signaling pathways in patients with high NR6A1 ***6A1 promoted cell proliferation,invasiveness,migration,and malignant tumor formation and growth in vitro and in ***:An effective prognostic model for HCC,based on a novel signature of 10 immune-related genes,was ***6A1 was up-regulated in HCC and was associated with a poor prognosis of ***6A1 promoted cell proliferation,migration,and growth of HCC,most likely through the cell cycle,mTOR,WNT,and ERBB signaling pathways.