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CAFs分泌的PDGFC通过PI3K-mTOR信号通路促进乳腺癌细胞对阿霉素耐药

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中国药理学通报 2024年第5期40卷 833-839页

作者：董浩王雪洁段万理盛智梅史立宏张宝刚山东第二医科大学临床病理系山东潍坊261042 山东第二医科大学附属医院病理科山东潍坊261041 山东第二医科大学康复医学院山东潍坊261053

目的探讨肿瘤相关成纤维细胞(cancer-associated fibroblasts,CAFs)来源的血小板衍生生长因子C(platelet derived growth factor C,PDGFC)是否促进乳腺癌细胞对阿霉素(doxorubicin,DOX)耐药并探讨其机制。方法原代提取CAFs和正常成纤维细胞(normal fibroblasts,NFs)并获取CAFs和NFs的条件培养基(conditional medium,CM),观察CAFs-CM是否影响乳腺癌细胞对DOX的敏感性;检测CAFs及其条件培养基中PDGFC的表达;Western blot检测乳腺癌细胞凋亡相关蛋白BAX/BCL2、耐药蛋白ABCG2、线粒体膜蛋白TOM20与COXⅣ及信号蛋白(p-)PI3K与(p-)mTOR的表达;DCFH-DA荧光染色、JC-1试剂盒分别检测乳腺癌细胞内ROS水平与线粒体膜电位。结果CAFs-CM减少乳腺癌细胞中DOX的含量,诱导对DOX耐药,同时乳腺癌细胞中BAX减少,Bcl2增加,ROS减少,线粒体膜电位升高及线粒体膜蛋白TOM20与COXⅣ表达增加。进一步研究发现,CAFs及CAFs-CM中PDGFC均高表达,重组人PDGFC产生了与CAFs-CM相似的促进乳腺癌细胞对DOX耐药的作用,PDGFRα的特异性抑制剂则显著抑制了CAF-CM诱导的耐药;进一步机制研究表明,CAFs分泌的PDGFC通过激活PI3K-mTOR信号通路诱导了乳腺癌细胞耐药。结论CAFs分泌的PDGFC通过PI3K-mTOR通路促进乳腺癌细胞对DOX耐药,这一发现为开发靶向CAFs的抗癌药物提供了新的思路。

关键词：肿瘤相关成纤维细胞血小板衍生生长因子C 乳腺癌耐药线粒体 PI3K-mTOR

Hydrogen Peroxide in Plants:a Versatile Molecule of the Reactive Oxygen Species Network

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Journal of Integrative Plant Biology 2008年第1期50卷 2-18页

作者： li-Juan Quan Bo Zhang Wei-Wei shi hong-Yu li MOE Key Laboratory of Arid and Grassland Ecology School of Life Sciences Lanzhou University Lanzhou 730000 China

Plants often face the challenge of severe environmental conditions, which include various biotic and abiotic stresses that exert adverse effects on plant growth and development. During evolution, plants have evolved complex regulatory mechanisms to adapt to various environmental stressors. One of the consequences of stress is an increase in the cellular concentration of reactive oxygen species （ROS）, which are subsequently converted to hydrogen peroxide （H2O2）. Even under normal conditions, higher plants produce ROS during metabolic processes. Excess concentrations of ROS result in oxidative damage to or the apoptotic death of cells. Development of an antioxidant defense system in plants protects them against oxidative stress damage. These ROS and, more particularly, H2O2, play versatile roles in normal plant physiological processes and in resistance to stresses. Recently, H2O2 has been regarded as a signaling molecule and regulator of the expression of some genes in cells. This review describes various aspects of H2O2 function, generation and scavenging, gene regulation and cross-links with other physiological molecules during plant growth, development and resistance responses.

关键词： antioxidant system gene regulation hydrogen peroxide (H2O2) reactive oxygen species (ROS) signaling molecule.

Boosting reversible charging of li-ion batteries at low temperatures by a synergy of propylene carbonate-based electrolyte and defective graphite

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Nano Research 2024年第3期17卷 1491-1499页

作者： Yingqiang Wu Jiao Zhang Jinli liu li Sheng Bo Zhang limin Wang Siqi shi li Wang hong Xu Xiangming He Institute of Nuclear and New Energy Technology Tsinghua UniversityBeijing 100084China State Key Laboratory of Rare Earth Resource Utilization Changchun Institute of Applied ChemistryChinese Academy of Sciences(CAS)Changchun 130022China China National Quality Supervision and Inspection Center for Industrial Explosive Materials Nanjing University of Science and TechnologyNanjing 210094China School of Materials Science and Engineering Materials Genome InstituteShanghai UniversityShanghai 200444China

Propylene carbonate(PC)-based electrolytes have exhibited significant advantages in boosting the low-temperature discharging of graphite-based li-ion ***,it is still unclear whether they can improve the charging property and suppress lithium *** this topic is challenging due to the problem of electrochemical *** overcome this issue,we introduced graphite with phase *** results show that the pouch-type full batteries using PC-based electrolyte exhibit steady performance over 500 cycles and can be reversibly charged over 30 times at-20℃ with an average Coulombic efficiency of 99.95%,while the corresponding value for the conventional ethylene carbonate(EC)-based electrolyte sample is only 31.20%.This indicates that the use of PC-based electrolyte significantly suppresses lithium plating during low-temperature *** further demonstrate that the improved performance is mainly attributed to the unique solvation structure,where PF-6more anions participate in solvation,leading to the formation of a stable F-rich solid state electrolyte interface on the graphite surface and a lower reduction tendency of li+*** work inspires new ideas for the design of PC-based electrolytes for low-temperature charging and fast-charging batteries.

关键词： lithium-ion batteries graphite propylene carbonate low-temperature charging

High expression of hepatitis B virus based vector with reporter gene in hepatitis B virus infection system

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World Journal of Gastroenterology 2007年第17期13卷 2490-2495页

作者： shi-hong li Wen-Ge Huang Bing Huang Xi-Gu Chen Center ofExperimental Animals Sun Yat-sen University Guangzhou510080 Guangdong Province China State KeyLaboratory of Ophthalmology ZhongShanOphthalmic Center Sun Yat-sen University Guangzhou 510060Guangdong Province China

AIM: To construct a hepatitis B virus (HBV)-based vector with a reporter gene and to establish an HBV infection system to evaluate the availability of the vector. METHODS: The HBV-based vectors with green fluorescence protein (GFP) were packaged into the liver of immunodeficient mice through transfer and helper plasmid using hydrodynamic technology. Wild type HBV (wt HBV) was provided by plasmid MC2009. Primary human hepatocytes (PHH) were isolated and infected with recombinant HBV (rHBV) or wt HBV. GFP expression was monitored by confocal and flow cytometry. HBV DNA and HBV surface antigen (HBSAg) were analyzed by PCR and EliSA. RESULTS: 3 × 107 wt HBV copies/mL and 5 × 106 rHBV copies/mL were collected from mice serum. In the wt HBV infected group, HBV progeny was 2 × 107 copies/mL and HBSAg was 770 ng/mL. In the rHBV infected group, GFP fluorescence was detected on d 3 post-infection and over 85% of the parenchymal cells expressed green fluorescence on d 12 post-infection. Compared with wt HBV in the PHH infection system, no rHBV DNA or HBSAg were detected in PHH culture media. CONCLUSION: An effective HBV based vector was developed, which proved to be a useful HBV infection system. This vector and infection system can be applied to develop a therapeutic vector and study the HBV life cycle and viral pathogenesis.

关键词： Hepatitis B virus Primary human hepatocyte Transfer plasmid Helper plasmid Infection system

Pectate lyase is a factor in the adaptability for Heterodera glycines infecting tobacco

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Journal of Integrative Agriculture 2019年第3期18卷 618-626页

作者： TIAN Zhong-ling shi hong-li Munawar Maria ZHENG Jing-wu Laboratory of Plant Nematology Institute of BiotechnologyCollege of Agriculture&BiotechnologyZhejiang University School of Resource and Environmental Sciences Henan Institute of Science and Technology Key Lab of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture

The soybean cyst nematode, Heterodeara glycines, is a serious pathogen of soybean, and reported to be the host of a wide range of Fabaceae. In the present study, the host specificity and reproductivity of two populations of H. glycines collected from soybean and tobacco were identified and characterized. The comparative identity between β-1,4-endoglucanase, pectate lyase and chorismate mutase of H. glycines parasitizing on soybean and tobacco were 99, 97 and 98%, respectively. The qR T-PCR analysis indicated that the expression of pectate lyase 2 gene was significantly higher in second-stage juveniles of H. glycines Henan population parasitizing on tobacco than that of H. glycines Shanxi population parasitizing on soybean. In addition, the pectic acid content of cell wall was significantly higher(45%) in the roots of tobacco than the roots of soybean. Our results indicate that the changes in transcript parasitism genes may be a result of long-term evolution illustrating how a plant-parasitic nematode adapts to the host environment for optimal infestation and survival.

关键词： s: Heterodera glycines tobacco parasitism genes β-1,4-endoglucanase pectate lyase chorismate mutase pectic acid

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一体化复合电源系统方案设计

移动电源与车辆 2024年第3期55卷 19-22页

作者：史红利郑高峰吴东建郑州金阳电气有限公司河南郑州450199 空军驻郑州地区军事代表室河南郑州450006

本文介绍了一体化复合电源系统的结构组成,以及氢燃料电池发电、储能锂电池、电能变换与能量管理、电站一体化散热与热平衡管理、电站一体化监控、电站综合集成等分系统的详细设计。该系统集成了氢燃料电池、内燃发电机组/市电和锂电池... 详细信息

本文介绍了一体化复合电源系统的结构组成,以及氢燃料电池发电、储能锂电池、电能变换与能量管理、电站一体化散热与热平衡管理、电站一体化监控、电站综合集成等分系统的详细设计。该系统集成了氢燃料电池、内燃发电机组/市电和锂电池组等供电设备,并通过一体化整合设计与控制,从而实现了多种能源在不同应用场合的一体化供电输出。

关键词：一体化电源氢燃料电池储能锂电池电源系统

结肠镜下黏膜切除术与冷圈套息肉切除术治疗结肠息肉的疗效对比分析

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中国处方药 2024年第3期22卷 190-193页

作者：张媛石丽红唐成祥赵旋赵淼徐州医科大学第二附属医院消化内科江苏徐州221000

目的探讨结肠镜下黏膜切除术(endoscopic mucosal resection,EMR)与冷圈套息肉切除术(cold snare polypectomy,CSP)治疗结肠息肉的疗效。方法在2020年1月~2022年12月徐州医科大学第二附属医院诊治结肠息肉患者中选取120例作为研究对象,... 详细信息

目的探讨结肠镜下黏膜切除术(endoscopic mucosal resection,EMR)与冷圈套息肉切除术(cold snare polypectomy,CSP)治疗结肠息肉的疗效。方法在2020年1月~2022年12月徐州医科大学第二附属医院诊治结肠息肉患者中选取120例作为研究对象,根据治疗方法将其分为两组。两组均进行常规治疗,对照组(60例)采用EMR治疗,观察组(60例)采用CSP治疗,对比两组患者治疗效果、手术指标和并发症。结果观察组治疗有效率95.00%(57/60)高于对照组80.00%(48/60),差异有统计学意义(P0.05)。结论黏膜切除术与冷圈套息肉切除术治疗结肠息肉均有较好的治疗效果,术后并发症较少,安全有效,冷圈套息肉切除术手术时间方面具有一定的优势。

关键词：结肠镜下黏膜切除术冷圈套息肉切除术结肠息肉手术时间并发症

Fine Mapping and Cloning of the Grain Number Per-Panicle Gene (Gnp4) on Chromosome 4 in Rice (Oryza sativa L.)

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Agricultural Sciences in China 2011年第12期10卷 1825-1833页

作者： ZHANG Zhan-ying li Jin-jie YAO Guo-xin ZHANG hong-liang DOU Hui-jing shi hong-li SUN Xing-ming li Zi-chao Key Laboratory of Crop Heterosis and Utilization Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement ChinaAgricultural University Beijing 100193 P.R.China

Grain number per-panicle is one of the most important components for rice yield. Spikelets on the primary and secondary branches determine the grain number per-panicle in rice. In this study, we identified a natural mutant, gnp4, lack of lateral spikelet on the secondary branches in the field condition. In addition, the Gnp4 and Lax1-1 double mutant showed dramatically reduced secondary branches and spikelets in panicle at reproductive stage, and tillers at vegetative stage. By map-based cloning approach, and using four F2 segregating populations, the Gnp4 gene was finally mapped to a 10.7-kb region on the long arm of chromosome 4 in rice. In this region, only one gene was predicted, and genomic DNA sequencing of the 10.7-kb region showed no nucleotide differences between the mutant and wild type. Interestingly, we found that the methylation level of several cytosines in the promoter CpG islands region of the predicted gene in gnp4 were different from the wild type. Thus, we propose that the DNA methylation changes at these sites may induce to decrease expression level of Gnp4, consequently, resulting in phenotypic variation.

关键词： rice gnp4 laxl-1 grain number per-panicle methylation

Blood lead level in Chinese adults with and without coronary artery disease

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Journal of Geriatric Cardiology 2021年第11期18卷 857-866页

作者： shi-hong li hong-Ju ZHANG Xiao-Dong li Jian CUI Yu-Tong CHENG Qian WANG Su WANG Chayakrit Krittanawong Edward A El-Am Rody G.Bou Chaaya Xiang-Yu WU Wei GU hong-hong liU Xian-liang YAN Zhi-Zhong li shi-Wei YANG Tao SUN Department of Cardiology Beijing Anzhen HospitalCapital Medical UniversityBeijingChina Department of Echocardiography Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina Department of Cardiology Shougang Changzhi Steel HospitalChangzhiChina Department of Cardiology Baylor College of MedicineHoustonUSA Department of Medicine Indiana University School of MedicineIndianapolisUSA

BACKGROUND The Trial to Assess Chelation Therapy study found that edetate disodium(disodium ethylenediaminetet-raacetic acid)chelation therapy significantly reduced the incidence of cardiac events in stable post-myocardial infarction patients,and a body of epidemiological data has shown that accumulation of biologically active metals,such as lead and cadmium,is an important risk factor for cardiovascular ***,limited studies have focused on the relationship between angiograph-ically diagnosed coronary artery disease(CAD)and lead *** study compared blood lead level(BLL)in Chinese pa-tients with and without *** In this prospective,observational study,450 consecutive patients admitted to Beijing Anzhen Hospital with sus-pected CAD from November 1,2018,to January 30,2019,were *** patients underwent coronary angiography,and an experienced heart team calculated the SYNTAX scores(SXscore)for all available coronary *** were determined with atomic absorption spectrophotometry and compared between patients with angiographically diagnosed CAD and those without *** In total,343(76%)patients had CAD,of whom 42%had low(0−22),22%had intermediate(23−32),and 36%had high(≥33)*** were 36.8±16.95μg/L in patients with CAD and 31.2±15.75μg/L in those without CAD(P=0.003).When BLLs were categorized into three groups(low,middle,high),CAD prevalence increased with increasing BLLs(P<0.05).In the multivariate regression model,BLLs were associated with CAD(odds ratio(OR):1.023,95%confidence interval(CI):1.008−1.039;P=0.0017).OR in the high versus low BLL group was 2.36(95%CI:1.29−4.42,P=0.003).Furthermore,BLLs were independently associated with intermediate and high SXscore(adjusted OR:1.050,95%CI:1.036-1.066;P<0.0001).CONCLUSION BLLs were significantly associated with angiographically diagnosed ***,BLLs showed excel-lent predictive value for SXscore,especially for complex coronary artery lesions.

关键词： coronary Blood admitted