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Cultured human embryonic neocortical cells survive and grow in infarcted cavities of adult rat brains and interconnect with host brain

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Chinese Medical Journal 2005年第4期118卷 275-280页

作者： ZENGJin-sheng YUJian CUIChun-mei ZHAOZhan HONGHua shengwen-li TAOYu-qian liling HUANGRu-xun StrokeCenter DepartmentofNeurologyFirstUniversityHospitalSunYat-SenUniversityGuangzhou510080China

Background There are no reports on exnografting cultured human fetal neocortical cells in this infracted cavities of adult rat brains. This study was undertaken to observe whether cultured human cortical neurons and astrocytes can survive and grow in the infarcted cavities of adult rat brains and whether they interconnect with host brains. Methods The right middle cerebral artery was ligated distal to the striatal branches in 16 adult stroke-prone renovascular hypertensive rats. One week later, cultured cells from human embryonic cerebral cortexes were stereotaxically transferred to the infarcted cavity of 11 rats. The other 5 rats receiving sham transplants served as controls. For immunosuppression, all transplanted rats received intraperitoneal injection of cyclosporine A daily starting on the day of grafting. Immunohistochemistry for glial fibrillary acidic protein ( GFAP), synaptophysin, neurofilament, and microtubule associated protein-2 ( MAP-2) was performed on brain sections perfused in situ 8 weeks after transplantation. Results Grafts in the infarcted cavities of 6 of 10 surviving rats consisted of bands of neurons with an immature appearance, bundles of fibers, and GFAP-immunopositive astrocytes, which were unevenly distributed. The grafts were rich in synaptophysin, neurofilament, and MAP2-positive neurons with long processes. The graft/host border was diffuse with dendrites apparently bridging over to the host brain, into which neurofilament immunopositive fibers protruded. Conclusion Cultured human fetal brain cells inummodepressed rats and integrate with the host brain.

关键词： cerebral infarction exnograft human rats

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Direct regeneration of liNi_(0.5)Co_(0.2)Mn_(0.3)O_(2) cathode material from spent lithium-ion batteries

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Chinese Journal of Chemical Engineering 2021年第12期34卷 278-286页

作者： Xiaodong Tang Qiankun Guo Miaomiao Zhou shengwen Zhong Faculty of Materials Melallurgy and Chemistry Jiangxi University of Science and TechnologyGanzhou 341000China

At present,metal ions from spent lithium-ion batteries are mostly recovered by the acid leaching procedure,which unavoidably introduces potential pollutants to the ***,it is necessary to develop more direct and effective green recycling *** this research,a method for the direct regeneration of anode materials is reported,which includes the particles size reduction of recovered raw materials by jet milling and ball milling,followed by calcination at high temperature after lithium *** regenerated liNi_(0.5)Co_(0.2)Mn_(0.3)O_(2) single-crystal cathode material possessed a relatively ideal layered structure and a complete surface morphology when the lithium content was n(Ni+Co+Mn):n(li)=1:1.10 at a sintering temperature of 920 0 C,and a sintering time of 12 *** first discharge specific capacity was 154.87 mA·h·g^(-1) between 2.75 V and 4.2 V,with a capacity retention rate of 90% after 100 cycles.

关键词： Spent lithium-ion batteries liNi_(0.5)Co_(0.2)Mn_(0.3)O_(2)cathode material Direct regeneration

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Multiplex network reconstruction for the coupled spatial diffusion of infodemic and pandemic of COVID-19

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International Journal of Digital Earth 2021年第4期14卷 401-423页

作者： Xiaoqi Zhang Zi-Ke Zhang Wenbo Wang Donglin Hou Jiajing Xu Xinyue Ye shengwen li Dong Furen Economic and Social Development School Wuhan UniversityWuhanPeople’s Republic of China Institute of Economics Chinese Academy of Social ScienceBeijingPeople’s Republic of China College of Media and International Culture Zhejiang UniversityHangzhouPeople’s Republic of China Alibaba Research Center for Complexity Sciences Hangzhou Normal UniversityHangzhouPeople’s Republic of China New Jersey Institute of Technology University HeightsNewarkNJUSA Texas A&M University College StationTXUSA School of Geography and Information Engineering China University of GeosciencesWuhanPeople’s Republic of China

The pandemic of COVID-19 witnessed a massive infodemic with the public being bombarded with vast quantities of *** spreading of neutral and highly accurate reports can guide the public to self-protect and reduce the ***-and dis-information would intrigue panic and high exposure risk to *** the infodemic has attracted attentions from the academia,it is still not known to what degree and in which direction the information flows contribute to the COVID-19 *** fill the gap,we apply network reconstruction techniques to rebuild the hidden multiplex network of information and COVID-19 spreading by which we aim at quantifying the interaction between the propagation of information and the spatial outbreak of COVID-19,and delineate between the positive and negative impact of information on the *** differentiating the types of media that participated in the information process,we find that in the early stage of COVID-19 pandemic,infodemic does play a critical role to amplify the risk of virus outbreak in China and the risk is even larger for those highly developed *** to the old-fashion media,the new mobile platforms impose a greater risk to reinforce the positive feedback between infodemic and COVID-19 pandemic.

关键词： Infodemic COVID-19 multiplex network spatial social network

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The Potential Role of Powdery Mildew-Resistance Gene Pm40 in Chinese Wheat-Breeding Programs in the Post-Pro21 Era

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Engineering 2018年第4期4卷 500-506页

作者： shengwen Tang Yuting Hu Shengfu Zhong Peigao Luo Sichuan Provincial Key Laboratory of Plant Breeding and Genetics Sichuan Agricultural University Chengdu 611130 China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100193 China

Powdery mildew, which is caused by Blumeria graminis f. sp. tritici (Bgt), is an important leaf disease that affects wheat yield. Powdery mildew-resistance (Pm) gene Pm21 was first transferred into wheat in the 1980s, by translocating the Heuchera villosa chromosome arm 6VS to the wheat chromosome arm 6AL (6VS·6AL). Recently, new Bgt isolates that are virulent to Pm21 have been identified in some wheat fields, indicating that wheat breeders should be aware of the risk of deploying Pm21, although pathological details regarding these virulent isolates still remain to be discovered. Pm40 was identified and mapped on the wheat chromosome arm 7BS from several wheat lines developed from the progenies of a wild cross between wheat and Thinopyrum intermedium. Pm40 offers a broad spectrum of resistance to Bgt, which suggests that it is likely to provide potentially durable resistance. Cytological methods did not detect any large alien chromosomal segment in the wheat lines carrying Pm40. lines with Pm40 and promising agronomical traits have been released by several wheat-breeding programs in the past several years. Therefore, we believe that Pm40 will play a role in powdery mildew-resistance wheat breeding after Pm21 resistance is overcome by Bgt isolates. In addition, both Pm21 and Pm40 were derived from alien species, suggesting that the resistance genes derived from alien species are potentially more durable or effective than those identified from wheat.

关键词：小麦染色体白粉病种植技术

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深度学习在颅内出血CT中的研究进展

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中国医学物理学杂志 2023年第6期40卷 775-779页

作者：牛胜文李坤华重庆医科大学附属第二医院放射科重庆400010

颅内出血起病迅速,死亡率及致残率高,非对比增强CT是疑似颅内出血患者的首选检查方式。深度学习高效、精准,已广泛应用于颅内出血的影像学研究中,但也存在一些问题,本研究整理了近年来深度学习在颅内出血CT领域中的研究进展,就深度学习... 详细信息

颅内出血起病迅速,死亡率及致残率高,非对比增强CT是疑似颅内出血患者的首选检查方式。深度学习高效、精准,已广泛应用于颅内出血的影像学研究中,但也存在一些问题,本研究整理了近年来深度学习在颅内出血CT领域中的研究进展,就深度学习引入颅内出血影像学领域后所取得的成果与存在的不足展开综述,以期实现深度学习辅助颅内出血精准诊断的进一步突破。

关键词：颅内出血深度学习非对比增强CT 综述

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农业绿色发展模式的农业保险需求引致效应——基于荔枝种植户的经验分析

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中国农业资源与区划 2022年第8期43卷 1-9页

作者：李胜文谢云飞华南农业大学经济管理学院广东广州510642

[目的]研究农业绿色发展模式引致的农业风险规避需求及其特点,提出完善和推广农业保险、推进农业绿色发展的政策启示。[方法]文章将农户农业保险需求分为5个等级,以采用生物防控和非化学生长调节技术来衡量农业绿色发展模式,然后构建排... 详细信息

[目的]研究农业绿色发展模式引致的农业风险规避需求及其特点,提出完善和推广农业保险、推进农业绿色发展的政策启示。[方法]文章将农户农业保险需求分为5个等级,以采用生物防控和非化学生长调节技术来衡量农业绿色发展模式,然后构建排序Logit模型,利用2018—2019年国家荔枝龙眼产业技术体系的调查数据证明农业绿色发展模式和农业保险需求之间引致效应,并借助OLS、2SLS、liML和分位数回归法检验了模型的稳健性。[结果](1)排序模型中农业绿色发展模式的系数均在1%的显著性水平上显著,说明农户的农业绿色发展模式会引致显著的农业保险需求,年龄、种植经验、合作组织、种植规模的系数也显著不为0,体现出种植规模较大、种植时间较长、参加合作组织的非老年人农户对农业保险需求影响较明显;(2) 0.50分位数回归的农业绿色发展模式系数值最大,表明农业绿色发展模式引致的农业保险需求往往处于中等水平。[结论]政府政策应充分考虑和满足农户农业绿色发展模式中的风险规避需求,才能深入推进农业绿色发展;而促进农业绿色发展的农业保险费率标准与推广策略也需要充分考虑农户种植规模、种植经验、合作组织、农户年龄和中等水平的农业保险需求等因素。

关键词：农业绿色发展绿色种植农业保险需求排序Logit模型引致需求

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十字形钢管混凝土柱-H形钢梁框架节点抗震性能研究

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建筑结构学报 2022年第11期43卷 95-106页

作者：何子奇胡盛文李彬洋杨远龙成宇重庆大学山地城镇建设与新技术教育部重点实验室重庆400045 重庆大学土木工程学院重庆400045 高速铁路轨道技术国家重点实验室北京100081

为了研究十字形钢管混凝土柱-H形钢梁框架中节点的抗震性能和破坏机理,进行了6个缩尺比为1∶2的节点拟静力试验。观察节点的损伤过程及破坏模式,分析柱端荷载-位移滞回曲线、节点核心区剪力-剪切变形曲线、层间位移角组成、耗能能力及... 详细信息

为了研究十字形钢管混凝土柱-H形钢梁框架中节点的抗震性能和破坏机理,进行了6个缩尺比为1∶2的节点拟静力试验。观察节点的损伤过程及破坏模式,分析柱端荷载-位移滞回曲线、节点核心区剪力-剪切变形曲线、层间位移角组成、耗能能力及应力分布。采用ABAQUS软件建立钢管混凝土异形柱-H形钢梁框架节点的有限元分析模型,分析结果与试验结果吻合良好,并对节点核心区受剪承载力和节点刚度进行参数分析。研究结果表明:节点的滞回曲线饱满,延性系数介于2.63~4.45之间,等效黏滞阻尼系数介于0.202~0.241之间,节点域的变形和耗能能力较强;建立的有限元分析模型可用于模拟节点的抗震性能,有限元参数分析结果表明增加节点区钢管厚度可以明显提高核心区受剪承载力,增加竖向肋板尺寸可以有效提高节点刚度。为保证竖向肋板节点达到刚性节点要求,建议柱钢板宽厚比不大于30;竖向肋板翼缘外高度、翼缘内高度以及竖向肋板与梁翼缘连接长度分别不应小于梁翼缘宽度的30%、15%和150%;竖向肋板厚度不应小于梁翼缘厚度。

关键词：十字形钢管混凝土柱梁柱节点拟静力试验有限元分析抗震性能

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风电机组关键部件磨蚀现状及防治研究进展

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中国表面工程 2023年第1期36卷 30-43页

作者：李太江李巍刘立营李聚涛王彩侠李生文孙琦西安热工研究院有限公司西安710054

随着风电事业的迅猛发展,风电机组关键部件磨蚀问题日益突出,而关于该问题的全面综述鲜有报道,亟须系统分析总结风电机组磨蚀问题及防治手段,为解决长期困扰风电行业的磨蚀难题提供参考和建议.首先,阐述陆上及海上风电机组金属部件腐蚀... 详细信息

随着风电事业的迅猛发展,风电机组关键部件磨蚀问题日益突出,而关于该问题的全面综述鲜有报道,亟须系统分析总结风电机组磨蚀问题及防治手段,为解决长期困扰风电行业的磨蚀难题提供参考和建议.首先,阐述陆上及海上风电机组金属部件腐蚀磨蚀现状,统计国内典型区域非金属部件的磨蚀现状,分析其产生磨蚀损伤的机理,指出塔筒等金属结构件腐蚀磨蚀问题突出,叠加所在地的特殊区域环境,出现加速失效;齿轮等金属零部件的磨蚀损伤是进一步失效的诱发因素,表现出与承担功能相关的特有失效形式;非金属部件以叶片前缘的磨蚀最为突出,引起持续的发电量损失和维修费用激增.其次,分别综述风电机组金属部件和非金属部件的磨蚀防护技术,指出塔筒等结构件在普遍应用传统涂层保护的基础上,仍须探索包覆技术和附加防护装置等新的防护方式;叶片本体涂料防护技术相对成熟,但涂料防护和贴膜防护均无法满足叶片前缘全周期防护的要求.最后,分析总结风电机组关键部件磨蚀防治存在的主要技术问题,对后续的研究方向进行了探讨和展望,填补了风电机组关键部件磨蚀防治领域的综述空白.

关键词：塔筒叶片磨蚀现状及防治

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基于图像深度学习的零件加工特征信息提取方法

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中国机械工程 2022年第3期33卷 348-355页

作者：张胜文周曦李滨城程德俊陈文笛江苏科技大学机械工程学院镇江212100 江苏省船海机械装备先进制造重点实验室镇江212100

针对各类基于模型定义(MBD)的零件模型加工特征的信息集成问题,提出一种多层次提取架构的加工特征全息信息提取方法。通过对零件的结构特点进行分析,以具有制造语义且无法拆分的最简化特征实现加工特征的分类;在阐述提取策略的基础上,... 详细信息

针对各类基于模型定义(MBD)的零件模型加工特征的信息集成问题,提出一种多层次提取架构的加工特征全息信息提取方法。通过对零件的结构特点进行分析,以具有制造语义且无法拆分的最简化特征实现加工特征的分类;在阐述提取策略的基础上,构建了基于深度学习图像识别技术的加工特征分类器;依据MBD模型信息标注的特点快速定位、抽取加工特征的拓扑结构,并利用多角度捕捉降维方法将加工特征彩色图像化,在此基础上设计了加工特征多角度图像识别综合分析方法;基于查询视图对MBD模型的标注信息进行过滤,构建了加工特征几何信息双层过滤式提取方法。最后开发了加工特征全息信息提取软件,并以船用柴油机关键件为实例验证了该方法的有效性。

关键词：全息信息深度学习图像化处理特征识别过滤式提取

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Development of a miniaturized 3D organoid culture platform for ultra-high-throughput screening

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Journal of Molecular Cell Biology 2020年第8期12卷 630-643页

作者： Yuhong Du Xingnan li Qiankun Niu Xiulei Mo Min Qui Tingxuan Ma calvin J.Kuo Haian Fu Department of Pharmacology and Chemical Biology and Emory Chemical Biology Discovery Center Emory University School of MedicineAtlantaGAUSA Department of Medicine Division of HematologyStanford University School of MedicineStanfordCAUSA

The recent advent of robust methods to grow human tissues as 3D organoids allows us to recapitulate the 3D architecture of tumors in an in vitro setting and offers a new orthogonal approach for drug ***,organoid culturing with extracellular matrix to support 3D architecture has been challenging for high-throughput screening(HTS)-based drug discovery due to technical *** genetically engineered human colon organoids as a model system,here we report our effort to miniaturize such 3D organoid culture with extracellular matrix support in high-density plates to enable *** first established organoid culturing in a 384-well plate format and validated its application in a cell viability HTS assay by screening a 2036-compound *** further miniaturized the 3D organoid culturing in a 1536-well ultra-HTS format and demonstrated its robust performance for large-scale primary compound *** miniaturized organoid culturing method may be adapted to other types of *** leveraging the power of 3D organoid culture in a high-density plate format,we provide a physiologically relevant screening platform to model tumors to accelerate organoid-based research and drug discovery.

关键词： human colon organoids KRAS^G12D 3D culture 384-well plate high-throughput screening(HTS) 1536-well plate ultra-HTS(uHTS)

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