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Myo-inositol accelerates the metamorphosis from megalopa to crablet of Scylla paramamosain by modulating cuticle decomposition and reconstruction

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Aquaculture and Fisheries 2023年第6期8卷 695-705页

作者： Wenxiao Cui Hafiz Sohaib Ahmed Saqib Weifeng Gao Ruibo Wang Yang He Yang Yu Zhen lin Qingyu Zhang Yin Zhang shengkang li Huaiping Zheng Yueling Zhang Mhd Ikhwanuddin Hongyu Ma Guangdong Provincial Key Laboratory of Marine Biotechnology Shantou UniversityShantou515063China STU-UMT Joint Shellfish Research Laboratory Shantou UniversityShantou515063China Rongtong Agricultural Development(Guangzhou)Co. LtdGuangzhou510030China Institute of Tropical Aquaculture and Fisheries Universiti Malaysia TerengganuKuala Nerus21030Malaysia

The mud crab Scylla paramamosain is a key species in China due to its high nutritional value and great economic worth and has grown in popularity.Myo-inositol can modulate versatile physiological functions in aquatic animals.In the present study,S.paramamosain megalopa were given graded concentrations of myo-inositol(0,1,2,4,and 8 ppm)by water immersion to explore how their metamorphosis would be affected.The results showed that supplementing with myo-inositol remarkably increased transformation and survival rate from megalopa to crablet by at least 1.16 and 1.26 times,respectively.To decipher the molecular mechanism of how myo-inositol increases metamorphosis and survival rate,we further performed transcriptome-based gene expression profiling of both megalopa and crablet treated with myo-inositol.The integrative transcriptome analyses predicted that the differentially expressed genes(DEGs)were significantly enriched in chitinase activity,structural constituent of cuticle,and chitin binding,which are associated with the decomposition and reconstruction of cuticle.qPCR results confirmed that myo-inositol mediated gene expression levels of the above cuticle-related pathways.Considering the importance of the cuticle in exoskeleton formation and molting,it can be concluded that myo-inositol-induced changes in the cuticle decomposition and reconstruction might have accelerated the transformation from megalopa to crablet of S.paramamosain.Besides,numerous DEGs were significantly enriched in protein digestion and absorption,amino sugar and nucleotide sugar metabolism.It implies that myo-inositol may improve survival by regulating energy or nutrient absorption.Additionally,the accelerated metamorphosis by myo-inositol may improve survival from megalopa to crablet of S.paramamosain.Overall,this study will provide the first insights into the underlying molecular mechanisms by which myo-inositol increases metamorphosis and survival.

关键词： Myo-inositol Scylla paramamosain Cuticle decomposition and reconstruction Chitin Metamorphosis Survival

滇东南南温河钨矿床成因和成矿时代:来自矿床地质特征和Sm-Nd同位素年龄的证据

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地质通报 2024年第6期43卷 914-925页

作者：李顺庭祝新友路增龙张定才韩胜康蒲兴明李孝光中色紫金地质勘查(北京)有限责任公司北京100012 北京矿产地质研究院有限责任公司北京100012 文山麻栗坡紫金钨业集团有限公司云南文山663600

南温河钨矿是滇东南地区规模最大的白钨矿床。从加里东期开始,区内遭受了多期构造-变质作用,地质作用复杂,导致以往对矿床成因、成矿时代等关键问题一直存在不同认识。在详实的野外地质工作基础上,结合前人研究成果,认为其为岩浆-热液... 详细信息

南温河钨矿是滇东南地区规模最大的白钨矿床。从加里东期开始,区内遭受了多期构造-变质作用,地质作用复杂,导致以往对矿床成因、成矿时代等关键问题一直存在不同认识。在详实的野外地质工作基础上,结合前人研究成果,认为其为岩浆-热液控制的矽卡岩型白钨矿床;成矿热液是岩浆-热液过渡态流体,具体表现形式为“穿层”和“顺层”石英脉-长石石英脉,其控制了矽卡岩化和白钨矿化;目前勘探工作揭露的层状、似层状矽卡岩矿体为“浆-液过渡成矿流体”顺层交代的产物。获得南温河钨矿Sm-Nd同位素年龄为126±6.7 Ma(n=7,MSWD=0.33),与区内构造研究成果和其他年代学成果获得良好的印证,成矿作用与燕山晚期岩浆活动有关。结合矿床地质特征和Sm-Nd同位素年龄等证据,认为南温河钨矿是由燕山晚期岩浆-热液系统控制的矽卡岩型白钨矿床。

关键词：滇东南地区南温河钨矿矿床成因成矿年代学

从调控肺泡中性粒细胞凋亡探讨血必净注射液防治体外循环肺损伤的机制

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中华危重病急救医学 2024年第2期36卷 166-171页

作者：徐朝军张胜康张怡周代勇明润宇宋岚湖南中医药大学第一附属医院心胸外科长沙410007 湖南中医药大学医学院生物化学与分子生物学教研室长沙410208

目的从调控中性粒细胞(PMN)凋亡探讨血必净注射液对体外循环(CPB)所致急性肺损伤(Ali)的防治作用。方法按随机数字表法将30只雄性SD大鼠分为假手术组(Sham组)、CPB模型组(CPB组)、血必净预处理组(XBJ组),每组10只。CPB组和XBJ组CPB 60 ... 详细信息

目的从调控中性粒细胞(PMN)凋亡探讨血必净注射液对体外循环(CPB)所致急性肺损伤(Ali)的防治作用。方法按随机数字表法将30只雄性SD大鼠分为假手术组(Sham组)、CPB模型组(CPB组)、血必净预处理组(XBJ组),每组10只。CPB组和XBJ组CPB 60 min后停机;Sham组不行CPB。XBJ组CPB前2 h腹腔注射4 mL/kg血必净注射液;Sham组和CPB组注射等量生理盐水。CPB后4 h,取动脉血进行血气分析,计算呼吸指数(RI);取肺组织测定肺系数(li)和肺含水率。收集支气管肺泡灌洗液(BALF)获取PMN,检测天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)活性,并用流式细胞仪检测细胞凋亡率;实时荧光定量聚合酶链反应(RT-qPCR)检测微小RNA-142-3p(miR-142-3p)、FoxO1 mRNA表达;蛋白质免疫印迹试验(Western blotting)检测FoxO1蛋白表达。此外,将HL-60细胞分为对照寡核苷酸转染组、miR-142-3p模拟物转染组和miR-142-3p拮抗物转染组,转染48 h后双荧光素酶报告基因检测miR-142-3p与FoxO1结合的活性。结果与Sham组相比,CPB组大鼠RI、li及肺含水率显著升高,BALF获取PMN中caspase-3活性和细胞凋亡率显著降低,miR-142-3p表达下降,FoxO1蛋白表达增加。与CPB组比较,XBJ组大鼠RI、li、肺含水率显著降低〔RI:0.281±0.066比0.379±0.071,li:4.50±0.26比5.71±0.42,肺含水率:(80.31±3.25)%比(84.59±3.41)%,均Pli的作用。

关键词：体外循环急性肺损伤血必净注射液微小RNA-142-3p FoxO1 凋亡

基于πPS-FBG传感技术的激光超声水下铝板缺陷检测研究

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河南师范大学学报（自然科学版） 2024年第4期52卷 46-51,I0006页

作者：郭古青白盛康田亚莉宫廷孙小聪周月婷邱选兵李传亮太原科技大学应用科学学院山西省精密测量与在线检测装备工程研究中心山西省光场调控与融合应用技术创新中心太原030024

为实现水下金属构件在线服役状态的在线评估,搭建了一套基于激光超声和π相移光纤布拉格光栅(πPS-FBG)的水下板材缺陷检测系统.为了研究水下激光超声传播规律,以6061铝合金板材为测试样品,进行了不同水深下的激光超声探测实验.与空气... 详细信息

为实现水下金属构件在线服役状态的在线评估,搭建了一套基于激光超声和π相移光纤布拉格光栅(πPS-FBG)的水下板材缺陷检测系统.为了研究水下激光超声传播规律,以6061铝合金板材为测试样品,进行了不同水深下的激光超声探测实验.与空气中超声传播相比,实验发现水下πPS-FBG多接收到一个超声波信号,此信号是激光第一次与水面作用产生的纵波信号.随着激励源与πPS-FBG传感距离的增加,超声信号在时域上也延迟增加;πPS-FBG接收的激光激励的首个超声波信号的波速为2871.91 m/s,缺陷反射波的波速为2911.02 m/s,其与铝板中超声表面波的速度接近,两者的相对误差分别为1.3%和0.03%.改变激励源与缺陷的距离,发现缺陷的定位精度与缺陷距离无关,缺陷位置的定位相对误差随着距离的增大而逐渐减小.本研究为水下金属构件的在线服役状态检测提供了一种潜在应用技术(包括在南水北调工程中),对于提高水下结构的安全性和可靠性具有一定意义.

关键词：激光超声 π相移光纤布拉格光栅 6061铝合金无损检测缺陷定位南水北调工程

Impact of Water-Sediment Regulation on Variations of Amino Acids in the Middle-Lower Yellow River, China

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Journal of Ocean University of China 2020年第2期19卷 369-376页

作者： ZHANG Kun ZOU li DAI Qunying WANG Jian JIANG Xueyan liANG shengkang College of Environmental Science and Engineering Ocean University of ChinaQingdao 266100China Key Laboratory of Marine Environment and Ecology Ministry of EducationOcean University of ChinaQingdao 266100China SGS-CSTC Standards Technical Services(Qingdao)Co. Ltd.Qingdao 266101China Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationOcean University of ChinaQingdao 266100China

In order to examine the impacts of water-sediment regulation on regional carbon cycling,we collected water,particulate and sediment samples from the middle-lower Yellow River in late June and early July,2015 and analyzed their specific amino acids(AA),DOC,POC,and bacteria abundance.Summarized by 14 specific AA,the total hydrolysable AA(THAA),particulate AA(PAA),and sediment AA(SAA)varied in ranges of 2.29-9.05μmol L^-1,5.22-22.96μmol L^-1,and 81.7-137.19μg g^-1 dry weight.After the regulation,dissolved free AA(DFAA)decreased by 29%while DCAA increased by 72%.These variations suggested that DFAA were further degraded,while DCAA molecules were further activated.Meanwhile,PAA increased almost 4 times as many as those before regulation,and SAA increased as well.After regulation,the amounts of bioactive amino acids(Asp,Glu and Gly)increased in THAA but decreased in PAA,with little changes in SAA.The ratios of Asp/Gly in different phases increased after regulation,indicating the AA contributions were promoted by calcareous organisms rather than by siliceous organisms.Multiple correlation analysis showed that PAA was primary representatives of AA and organic carbon,followed by DCAA and POC.Moreover,bacterial reproduction played a key role in shaping the AA compositions and properties,followed by the redox condition and acid-base balance.The results of this study provided a clear evidence for the effects of water-sediment regulation on regional biogeochemistry of organic carbon in the middle-lower Yellow River.

关键词： the middle-lower Yellow River water-sediment regulation amino acids organic carbon

Total Maximum Allocated Loads on Stoichiometry of Nitrogen and Identification of Critical Form in Jiaozhou Bay, China

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Journal of Ocean University of China 2020年第3期19卷 622-632页

作者： liN Guohong SONG Xianli LU Dongliang li Keqiang liANG shengkang WANG Xiulin Key Laboratory of Marine Chemistry Theory and Technology(Ocean University of China) Ministry of EducationQingdao 266100China College of Material Science and Engineering Qingdao UniversityQingdao 266100China Marine Biology Institute of Shandong Province Qingdao 266104China Guangxi Key Laboratory of Marine Disaster in the Beibu Gulf Qinzhou UniversityQinzhou 535099China

Total pollutant load control management for total dissolved nitrogen(TDN) is an urgent task required to gain a good water quality status in Jiaozhou Bay(JZB), China. In this paper, the stoichiometry of multiform TDN on land-ocean interactions associated with marine biogeochemical reaction(LOIMBR) was studied by modeling the load-response relationship based on a three-dimensional water quality model of nitrogen in JZB. The results showed that the stoichiometry on LOIMBR of dissolved organic nitrogen(DON), NO3-N and NH4-N was 3:1:1, with one-third of the contribution on the concentration of dissolved inorganic nitrogen(DIN) in JZB for the land-based DON loads to DIN loads. Based on the stoichiometric relationship of nitrogen forms, the total maximum allocated load(TMAL) of equivalent TDN(ETDN) was approximately 5300 t a^-1 in JZB, equivalent to the TMAL of 5700, 5800 and 15600 t a^-1 for NH4-N, NO3-N and DON, respectively. According to the loads of ETDN, there were four outfalls overloaded in JZB in 2015, which lie in the head of the bay. In the four overloaded outfalls, besides NO3-N, NH4-N was the critical nitrogen control form for Moshui River, while DON for Dagu River and Haibo River. The results of numerical experiments further showed that JZB will achieve good water quality after 7 years by implementation of the 'different emission reduction' based on TMAL of ETDN, which is significantly better than 'equal percent removal'.

关键词： total dissolved nitrogen water quality stoichiometry total maximum allocated load Jiaozhou Bay

A nitrogen and phosphorus dynamic model of mesocosm pelagic ecosystem in the Jiaozhou Bay in China

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Acta Oceanologica Sinica 2008年第5期27卷 98-110页

作者： li Keqiang WANG Xiulin liANG shengkang SHI Xiaoyong ZHU Chenjian CHEN Hu College of Chemistry and Chemical Engineering Ocean University of China Qingdao 266100 China Key Laboratory of Marine Chemistry Theory and Technology of Ministry of Education Ocean University of China Qingdao 266100 China Marine Pollution Eco-chemistry Laboratory Ocean University of China Qingdao 266100 China National Oceanography Centre University of Southampton Southampton SO14 3ZE UK Qingdao Land of State Power Environment Engineering CO. LTD Qingdao 266071 China

A nitrogen and phosphorus dynamic model of mesocosm pelagic ecosystem was established according to the summary and synthesis of the models available, in which seven state variables （DIN, PO4-P, DON, DOP, phytoplankton, zooplankton and detritus） were included. Logically it had five modules--phytoplankton, zooplankton, dissolved inorganic nutrients, dissolved organic nutrients and detritus. The results showed that this model could simulate the variations of DIN, PO4-P, DON, DOP, POC and phytoplankton biomass in pelagic ecosystem in mesocosm properly, based on the site experiment data in the Jiaozhou Bay in the autumn of 1999 and the summer of 2000. Not only the logical structure but also the model parameters were feasible, and about 20 parameters were made to fit for the Jiaozhou Bay during the simulation. All of these are necessary to study the control mechanism of nutrients biogeochemical cycling in the Jiaozhou Bay and other China＇ s coastal waters.

关键词： Jiaozhou Bay nitrogen and phosphorus dynamic model mesocosm

Identification and Characterization of Gene SpDMRT99B and Its Sex-Biased Expression Profile in the Mud Crab,Scylla paramamosain

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Journal of Ocean University of China 2021年第6期20卷 1495-1504页

作者： ZHANG Yin FANG Shaobin liN Fei li shengkang ZHENG Huaiping ZHANG Yueling IKHWANUDDIN Mhd MA Hongyu Guangdong Provincial Key Laboratory of Marine Biotechnology Institute of Marine SciencesShantou UniversityShantou 515063China STU-UMT Joint Shellfish Research Laboratory Shantou UniversityShantou 515063China Institute of Tropical Aquaculture Universiti Malaysia TerengganuKuala Terengganu 21030Malaysia

The doublesex and mab-3 related transcription factor(DMRT)gene family is conserved from invertebrates to humans.The functions of DMRT are mainly involved in sex development and the formation of many tissues and organs.In this study,a DM(Doublesex/Mab-3-domain gene was identified in the mud crab Scylla paramamosain,and was named SpDMRT99B because of its many similarities to arthropod DMRT99B and phylogenetically close relationship with arthropod DMRT99B.The cDNA of SpDMRT99B gene is 1249 bp in length,encoding 224 amino acids.From 254 bp to 928 bp there is a conserved DM domain.No transmembrane domain was identified.Through multiple amino acid alignment and phylogenetic tree analysis,the closest gene to SpDMRT99B is Eriocheir sinensis DMRT99B,followed by Macrobrachium rosenbergii and Drosophila melanogaster DMRT99B.The expressions of the gene were characterized in different tissues of female and male crabs during early development period of crab individuals,as well as in different development periods of gonads.The results showed that SpDMRT99B gene is significantly highly expressed in testis than in ovary and other tissues.The expression level of SpDMRT99B in testis at different stages is significantly higher than that in ovary,and it is particularly highly expressed in immature testis.In early developmental stages of larvae,the expressions of SpDMRT99B remain at a low level and reach a peak at zoea stage I when the body segments shape up.It is speculated that SpDMRT99B gene might be involved in the gonadal development process and somitogenesis of S.paramamosain.

关键词： Scylla paramamosain DM domain gene SpDMRT99B sex determination/differentiation early development stage

CcTV注射法:全面部注射填充透明质酸钠凝胶的微创美容方法

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中华医学美学美容杂志 2024年第2期30卷 173-176页

作者：李方伟郭晓波陈光宇宋建星陈柯洪伟谭晓燕吴晓军罗盛康广东省第二人民医院整形科广州510317 杭州芙艾医疗美容门诊部杭州310014 中国医学科学院整形外科医院注射美容中心北京100144 同济大学附属上海市第四人民医院整形外科上海200041 广州美莱医疗美容门诊部广州510010 朗姿集团晶肤医疗美容连锁成都610014 杭州整形医院整形美容外科杭州310014 上海交通大学医学院附属第九人民医院大沽路整形美容门诊部上海200041

注射用透明质酸钠凝胶是微创注射美容项目中最常用的填充剂,目前缺乏适合中国人全面部注射填充方法。经过临床实践,总结出一套适合中国人全面部注射填充的方法,定义为CcTV注射法。CcTV注射法将面部分为4区进行注射:C区(颞部、颧部、鼻... 详细信息

注射用透明质酸钠凝胶是微创注射美容项目中最常用的填充剂,目前缺乏适合中国人全面部注射填充方法。经过临床实践,总结出一套适合中国人全面部注射填充的方法,定义为CcTV注射法。CcTV注射法将面部分为4区进行注射:C区(颞部、颧部、鼻唇沟区及相关韧带)、c区(眶周,包括眉弓、泪沟、眶周相关韧带)、T区(额部、鼻部)、V区(颏部、下颌缘、颊部)。该方法结合中国人面部衰老特点、面部韧带注射提升技术、外轮廓注射填充技术等,内容包括CcTV注射法的注射目的、注射剂量、注射定位、注射技巧,可作为临床进行全面部注射填充美容的参考资料。

关键词：注射透明质酸填充美容医学微创手术