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Species clarification of the prize medicinal Ganoderma mushroom“Lingzhi”

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Fungal Diversity 2012年第5期56卷 49-62页

作者： Yun Cao sheng-hua wu Yu-Cheng Dai State Key Laboratory of Forest and Soil Ecology Institute of Applied EcologyChinese Academy of SciencesShenyang 110164China Graduate University of the Chinese Academy of Sciences Beijing 100049China Department of Botany National Museum of Natural ScienceTaichung 404Taiwan Department of Plant Pathology National Chunghsing UniversityTaichung 402Taiwan e-mail:shwu@mail.nmns.edu.tw Institute of Microbiology Beijing Forestry UniversityBeijing 100083China e-mail:yuchengd@***

“Lingzhi”is a mushroom that has been renowned in China for more than 2,000 years because of its claimed medicinal properties plus its symbolic fortune.“Lingzhi”has high economic value mostly as a dietary supplement in the modern market especially in East Asia,and its medicinal functions have become a hot study *** over a century,the highly prized medicinal fungus,known as“Lingzhi”in East Asia,has been assigned to Ganoderma lucidum,a species originally described from *** studies in recent years have revealed that the commercially cultivated‘***’(“Lingzhi”)in East Asia is a different species from the true *** present study aims to clarify the species identity of“Lingzhi”based on morphological studies and analysis of rDNA nuc-ITS sequences,and additional gene fragments of mt-SSU,RPB1,RPB2,and TEF1-αof“Lingzhi”were *** Ganoderma species that mostly resemble“Lingzhi”in phylogeny and/or morphology were included for *** propose a new species *** for“Lingzhi”,which has an East Asia *** most striking characteristics which differentiate *** from *** are the presence of melanoid bands in the context,a yellow pore surface and thick dissepiments(80–120μm)at *** is most closely related to *** in phylogeny and is from North *** flexipes,***,***,*** and‘***’,are also closely related with *** and are reported from *** species are compared and discussed.‘Ganoderma tsuage’reported from China is determined as conspecific with ***,hence the distribution of *** extends from Europe to northeastern China.

关键词： Ganodermataceae Ganoderma lingzhi G lucidum Medicinal fungus Phylogeny Taxonomy

Prediction of Forming Limit Diagrams for Materials with HCP Structure

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Acta Metallurgica Sinica(English Letters) 2015年第12期28卷 1442-1451页

作者： sheng-hua wu Nan-Nan Song Francisco M.Andrade Pires Abel D.Santos Faculty of Engineering University of Porto INEGI University of Porto

The forming limit diagram（FLD） is an important tool to be used when characterizing the formability of metallic sheets used in metal forming processes. Experimental measurement and determination of the FLD is timeconsuming and therefore the analytical prediction based on theory of plasticity and instability criteria allows a direct and efficient methodology to obtain critical values at different loading paths, thus carrying significant practical ***, the accuracy of the plastic instability prediction is strongly dependent on the choice of the material constitutive model [1–3]. Particularly for materials with hexagonal close packed（HCP） crystallographic structure, they have a very limited number of active slip systems at room temperature and demonstrate a strong asymmetry between yielding in tension and compression [4, 5]. Not only the magnitude of the yield locus changes, but also the shape of the yield surface is evolving during the plastic deformation [4]. Conventional phenomenological constitutive models of plasticity fail to capture this unconventional mechanical behavior [4, 6]. Cazacu and Plunkett [6] have proposed generic yield criteria, by using the transformed principal stress, to account for the initial plastic anisotropy and strength differential（SD） effect simultaneously. In this contribution, a generic FLD MATLAB script was developed based on Marciniak–Kuczynski analytical theory and applied to predict the localized necking. The influence of asymmetrical effect on the FLD was evaluated. Several yield functions such as von Mises, Hill, Barlat89, and Cazacu06 were incorporated into analysis. The paper also presents and discusses the influence of different hardening laws on the formability of materials with HCP crystal structures. The findings indicate that the plastic instability theory coupled with Cazacu model can adequately predict the onset of localized necking for HCP materials under different strain paths.

关键词： Forming limit diagram(FLD) Marciniak–Kuczynski analysis Plastic instability Yield function Hardening law

Species diversity,taxonomy and multi-gene phylogeny of phlebioid clade(Phanerochaetaceae,Irpicaceae,Meruliaceae)of Polyporales

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Fungal Diversity 2021年第6期111卷 337-442页

作者： Che-Chih Chen Chi-Yu Chen sheng-hua wu Department of Plant Pathology National Chung Hsing UniversityTaichung 402202Taiwan Department of Biology National Museum of Natural ScienceTaichung 404605Taiwan Biodiversity Research Center Academia SinicaTaipei 115201Taiwan

The phlebioid clade(Polyporales,Basidiomycota)accommodates numerous species of corticioid and polyporoid fungi of the Phanerochaetaceae,Irpicaceae,and *** present study used morphological and phylogenetic approaches to revise the generic classification of the phlebioid clade and survey species *** phylogenetic analyses were performed using sequences of multiple genes,including the nuc rDNA ITS1-5.8S-ITS2(ITS),the D1-D2 domains of 28S rDNA(28S),the RNA polymerase II largest subunit(rpb1),the RNA polymerase II second largest subunit(rpb2),and the translation elongation factor 1-α(tef1).We overall recognize 57 genera including six new ones(Alboefibula,Cremeoderma,Gelatinofungus,Luteochaete,Phanerochaetella and Quasiphlebia).We describe 26 new species belonging to 15 genera(Alboefibula bambusicola,***,Crustodontia taiwanensis,Cytidiella albomarginata,Efibula matsuensis,***-gida,***,Gelatinofungus brunneus,Hydnophlebia aurantia,***,Irpex lenis,Mycoaciella efibulata,Phanerochaete alpina,***,***,***,***,***,Phanerochaetella formosana,Phlebiopsis odontoidea,***,Quasiphlebia densa,Rhizochaete chinensis,Roseograndinia jilinensis,***,and Scopuloides allantoidea),and present 18 new combinations belonging to 12 genera(Cremeoderma unicum,Crustodontia nigrodontea,***,Cytidiella albida,Efibula intertexta,Hydnophlebia alachuana,Irpex laceratus,***,***,Luteochaete subglobosa,Luteoporia lutea,Phanerochaetella angustocystidiata,***,***,***,Phlebiopsis alba,Rhizochaete lutea,Scopuloides dimorpha).Descriptions,illustrations and notes of new species and some new records are provided,as well as identification keys to genera of each family.

关键词： Basidiomycota Classification Systematics Wood-decay fungi 50 new taxa

Characteristics of respiratory syncytial virus-induced bronchiolitis co-infection with Mycoplasma pneumoniae and add-on therapy with montelukast

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World Journal of Pediatrics 2016年第1期12卷 88-95页

作者： sheng-hua wu Xiao-Qing Chen Xia Kong Pei-Ling Yin Ling Dong Pei-Yuan Liao Jia-Ming wu Department of Pediatrics First Affi liated Hospital of Nanjing Medical University300 Guangzhou RoadNanjing 210029China Department of Pediatrics Jiangsu Maternity and Children Healthcare HospitalNanjing 210036China Department of Pediatrics Nanjing First Hospital Affi liated to Nanjing Medical UniversityNanjing 210006China Department of Pediatrics Central Hospital of TengzhouTengzhou 277500China Department of Pediatrics Qidong People's Hospital753 Central Jianghai RoadQidong 226200China

Background:The influence of Mycoplasma pneumoniae(MP)infection on bronchiolitis remains ***,reports on the efficacies of leukotriene receptor antagonists in the treatment of bronchiolitis have been ***:Children with respiratory syncytial virus(RSV)-induced bronchiolitis were divided into two groups:RSV+MP group and RSV *** group was randomly divided into two subgroups:one received routine and placebo treatment,while the other received routine and montelukast treatment for 9 *** cumulative numbers of wheezing episodes and recurrent respiratory tract infections were *** parameters were ***:Patients in the RSV+MP group exhibited an older average age,fever,more frequent flaky and patchy shadows in chest X-rays,more frequent extrapulmonary manifestations,and longer hospital stays compared with patients in the RSV ***,higher baseline blood eosinophil counts,eosinophil cationic protein(ECP),total immunoglobulin E(IgE),interleukin(IL)-4,IL-5,IL-4/interferon-γratios,leukotriene(LT)B4,and LTC4,and lower baseline lipoxin A4(LXA4)/LTB4 ratios were observed in the RSV+MP group compared with the RSV *** treatment decreased the cumulative numbers of recurrent wheezing episodes and recurrent respiratory tract infections at 9 and 12 *** efficacy may be related to the montelukast-induced reductions in peripheral eosinophil counts,ECP and total IgE,as well as the montelukast-dependent recovery in T helper(Th)1/Th2 balance and LXA4/LTB4 ratios in children with ***:RSV bronchiolitis with MP infection was associated with clinical and laboratory features that differed from those of RSV bronchiolitis without MP ***-on therapy with montelukast for 9 months was benefi cial for children with bronchiolitis at 9 and 12 months after the initiation of treatment.

关键词： bronchiolitis leukotrienes montelukast Mycoplasma pneumoniae respiratory syncytial virus

Effect of Nitrogen Applied Before Transplanting on NUE in Rice

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Agricultural Sciences in China 2007年第7期6卷 842-848页

作者： ZHENG Yong-mei DING Yan-feng WANG Qiang-sheng LI Gang-hua wu Hao YUAN Qi WANG Hui-zhi WANG Shao-hua Key Laboratory of Crop Growth Regulation Ministry of Agriculture/Nanjing Agricultural University Nanjing 210095 P.R.China

Nitrogen （N） application before transplanting, where N fertilizers are applied in seedling-bed and carried to the paddy field with seedlings, is a novel method proposed in this article aiming for improving nitrogen utilization efficiency （NUE） in rice. The effect of this method on mineral N distribution in the rhizosphere soil was investigated in a field experiment with a japonica variety, Ningjing 2, in seasons of 2004 and 2005. There were four levels of N applied 16 h before transplanting： zero N （NO）, 207 kg ha^-1 （NL）, 310.5 kg ha^-1 （NM）, and 414 kg ha^-1 （NH）. The result indicated that N fertilizer before transplantation had positive effect of increasing mineral N content in the rhizosphere soil of rice. Generally, N content in the rhizosphere soil of rice tended to increase with the amount of N fertilizer before transplanting, with the NH treatment having the largest effect. Additionally, N fertilizer before transplanting had significant influence on rice NUE and grain yield. Compared with other treatments, the NM treatment showed the largest influence, with basal-tillering NUE, total NUE, and grain yield being 15%, 12%, and 529.5 kg ha^-1 higher than those of NO treatment. This result indicated that N fertilizer before transplantation had positive effect on mineral N distribution in the rhizosphere soil of rice, thus improving NUE and grain yield.

关键词： rice nitrogen (N) application before transplanting nitrogen utilization efficiency (NUE) grain yield

Global diversity and taxonomy of the Inonotus linteus complex(Hymenochaetales,Basidiomycota):Sanghuangporus ***.,Tropicoporus excentrodendri and *** *** ***.,and 17 new combinations

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Fungal Diversity 2016年第2期77卷 335-347页

作者： Li-Wei Zhou Josef Vlasák Cony Decock Addisu Assefa Jan Stenlid Dawit Abate sheng-hua wu Yu-Cheng Dai State Key Laboratory of Forest and Soil Ecology Institute of Applied EcologyChinese Academy of SciencesShenyang 110164China Department of Biology National Museum of Natural ScienceTaichung 404TaiwanRepublic of China Biology Centre of the Academy of Sciences of the Czech Republic Branišovská3137005ČeskéBudějoviceCzech Republic Mycothèque de l’Universitécatholique de Louvain(MUCL BCCMTM)Earth and Life Institute–Microbiology(ELIM)Universitécatholique de LouvainCroix du Sud 2 bte L7.05.061348 Louvain-la-NeuveBelgium Department of Biology Madawalabu UniversityP.O B.247Bale RobeEthiopia Department of Forest Mycology and Plant Pathology Swedish University of Agricultural SciencesP.O.B.702675007 UppsalaSweden Department of Microbial Cellular and Molecular BiologyAddis Ababa UniversityP.O.B.1176Addis AbabaEthiopia

Although Inonotus linteus complex is placed in the genusInonotus,its perennial basidiocarps with a dimitic hyphal system,at least in the trama,distinguish the complex from other species in the genus,which have an annual habit and a monomitic hyphal *** species number of Inonotus linteus complex has been increased in China and tropical America in recent ***,whether species in this complex belong to Inonotus has not specifically been ad*** explore the phylogenetic placement of species of Inonotus linteus complex,we performed a comprehensive study using morphological and phylogenetic data based on global ***(53 with 13 new)and ITS(70 with 18 new)datasets were used to produce the phylogenetic *** into consideration the phylogenies inferred from the nLSU and ITS datasets,Inonotus is a polyphyletic genus comprising at least three *** A,the core Inonotus clade,contains the generic type,Inonotus hispidus,while Clades B and C comprise species from the Inonotus linteus *** and phylogenetic evidence indicates that Clades B and C are new genera,and Sanghuangporus and Tropicoporus are introduced in this *** species are transferred to Sanghuangporus and seven to *** excentrodendri and *** *** described,and their distinctive characters are *** to the two new genera and the Inonotus sensu stricto,and to species of each new genus are provided.

关键词： Polypore Hymenochaetaceae Morphology nLSU ITS

MyD88/PI3-K/NF-κB pathway mediates the antagonism of lipoxin A_4 on LPS-induced synthesis of interleukins in endothelial cells

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Journal of Microbiology and Immunology 2006年第2期4卷 125-130页

作者： sheng hua wu PEI YUAN LIAO LING DONG Department of Pediatrics the First Affiliated Hospital of Nanjing Medical University Nanjing P. R. China Department of Pediatrics Central Hospital of Tengzhou Tengzhou P. R. China

In order to investigate whether lipoxin A4 （LXA4） has an antagonistic effect on lipopolysaccharide （LPS）-induced synthesis of interleukin （IL）-β3, IL-6 and IL-8 in rat pulmonary microvascular endothelial cells （PMVEC）, and to explore the molecular mechanisms of signal pathway in LXA4 actions, cultured PMVEC were treated with LPS, with or without preincubation with LXA4. Proteins of IL-β3, IL-6 and IL-8 in supernatant were analyzed by enzyme-linked immunosorbent assay （ELISA）. Expressions of mRNA of IL-β3, IL-6 and IL-8 were determined by RT-PCR. Expressions of phosphorylation of phosphoinositide 3-kinase （PI3-K） and myeloid differentiation factor 88 （MyD88） were analyzed by Western blot. Activities of DNA-binding of nuclear factor-kappa B （NF-κB） and activator protein-1 （AP-1） were measured by electrophoretic mobility shift assay （EMSA）. The results showed that LPS induced production of IL-β3, IL-6 and IL-8 in rat PMVEC via MyD88/PI3-K/NF-κB and AP-1 pathway-dependent signal transduction. LPS-stimulated expression of PI3-K, activities of NF-κB and AP-1, secretion of protein and expression of mRNA of IL-β3, IL-6 and IL-8 but not MyD88 expression in PMVEC were inhibited by LXA4 in a dose-dependent manner. In conclusion, LXA4 inhibits synthesis of IL-β3, IL-6 and IL-8 by down-regulation of PI3-K/NF-κB and AP-1 signal pathway in PMVEC.

关键词： Lipoxin Lipopolysaccharide Interleukin Nuclear factor-κB Endothelial ceils

FIRST ORDER WALL VORTICITY FORMULA UNDER IRREGULAR MESH

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Journal of Hydrodynamics 1994年第3期6卷 47-52页

作者： Liao hua-sheng, wu Chi-gongChengdu University of Science and Technology, Chengdu 610065, P. R. China

In the engineering applications, flow problems with complicated geometrical shape of boundaries are of ten happen. Their boundary conditions should be given in precisely when doing the simulation of these type of flows because the regions near to the boundaries generally play an important role on the defined solutions. Using the vorticity-stream function form of the N-S equations as governing equations in the flow field simulation with irregular mesh, the determining/calculating wall vorticity under irregular mesh is very important. In this paper, one first order formula of wall vorticity under irregular mesh was derived based on the 2-D Taylor expansion and was tested numerically through an example of a flow with the Z type, shape of boundaries. A satisfactory result was found which was compared with one obtained by FEM.

关键词： vorticity-stream functions irregular mesh simulation.

Studies on the Interaction of 12-Tungstophosphoric Acid and Diethylene Glycol

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Chemical Research in Chinese Universities 1993年第4期9卷 317-323页

作者： ZHAO Yu-sheng, hua Shi-ying , wu Tong-hao and WEI Quan (Department of Chemistry, Jilin University, Changchun, 130023) Worked in Institute of Theoretical Chemistry, Jilin University, Changchun.

The homogeneous system of 12-tungstophosphoric acid and diethylene glycol was studied using IR and NMR spectra. It was found that the protons in 12-tungstophosphoric acid formed proton oxonium ions with the hydroxyl oxygen in diethylene glycol by hydrogen-bonds, and the formed proton oxonium ions could react with the terminal oxygens of heteropoly anions. The dehydration-cyclization mechanism of diethylene glycol in the presence of heteropoly acid was also proposed.

关键词： IR NMR Diethylene glycol Proton oxonium ions