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Gene expression profiling of the whitefly （Bemisia tabaci） Middle East- Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco

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Insect Science 2011年第1期18卷 11-22页

作者： Jun-Min Li Yong-Ming Ruan Fang-Fang Li shu-sheng Liu Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Insect Sciences Zhejiang University Hangzhou College of Chemistry and Life Sciences Zhejiang Normal University Jinhua Zhejiang Province China

Begomoviruses are exclusively transmitted by whitefly （Bemisia tabaci） in a circulative, non-propagative manner. The influences of begomoviruses on whitefly vector are complex with both direct and indirect effects. However, the molecular mechanisms underlying these effects are poorly understood and the transcriptional profiles of whitefly on healthy and virus-infected plants have not yet been studied. Using suppression subtractive hybridization, we investigated the differentially expressed genes in whitefly Middle East - Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus （TYLCCNV） infected tobacco. From the forward cDNA library, 124 differentially expressed expression sequence tags （ESTs） were obtained which represent up-regulated genes in the whiteflies feeding on the infected tobacco. From the reverse library, 112 ESTs were isolated which represent down-regulated genes. Among the up-regulated genes, we identified several genes that are probably involved in direct interaction between whitefly and TYLCCN~, including a 26/29-kDa proteinase that appears to participate in the elimination of foreign proteins, heparan sulfate proteoglycan which mediates the entry of several viruses into host cells and two genes of Rickettsia-a secondary endosymbiotic bacterium of whitefly. In addition, we identified a number of genes involved in metabolism, transcription and translation which might be the result of indirect effects of TYLCCNV on the whitefly via host plants. Collectively, our results suggest that TYLCCNV-infected tobacco changes the gene expression profiles of whitefly via both direct and indirect interactions. This study revealed a number of genes involved in whitefly-TYLCCNV-tobacco interactions and provided useful information for future study on this complex system.

关键词： begomovirus Bemisia tabaci suppression subtractive hybridization Tomatoyellow leaf curl China virus whitefly

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High Performance Affinity Chromatography of Antithrombin III Based on Monodisperse Poly (glycidyl methacrylate) Beads

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Chinese Chemical Letters 2001年第5期12卷 443-446页

作者： Ying Xin ZHAO Di Hua SHANGGUAN Rui ZHAO Tian sheng shu Guo Quan LIU Center for Molecular Science Institute of Chemistry Chinese Academy of ScienceBeijing 100080

A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse, non-porous, cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide, heparin was linked covalently to amino-PGMA-beads, which was prepared by amination of PGMA. The adsorbent obtained exhibits high binding activity to antithrombin III (ATIII), good resolution and excellent mechanical properties and can be used under high flow rate.

关键词： High performance affinity chromatography antithrombin III heparin

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Genomic and transcriptomic analyses reveal metabolic complementarity between whiteflies and their symbionts

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Insect Science 2022年第2期29卷 539-549页

作者： Dan-Tong Zhu Qiong Rao Chi Zou Fei-Xue Ban Juan-Juan Zhao shu-sheng Liu Ministry of Agriculture Key Lab of Molecular Biology of Crop Pathogens and Insects Institute of Insect SciencesZhejiang UniversityHangzhou310029 China School of Agriculture and Food Science Zhejiang Agriculture and Forestry UniversityLin'anHangzhou311300 China

Nutritional mutualism between insects and symbiotic bacteria is *** various sap-feeding whitefly species within the Bemisia tabaci complex associate with the same obligate symbiont(Portiera)and multiple secondary *** is often assumed that some of the symbionts residing in the whiteflies play crucial roles in the nutritional physiology of their insect *** effort has been made to understand the functions of the whitefly symbionts,the metabolic complementarity offered by these symbionts to the hosts is not yet well *** examined two secondary symbionts,Arsenophonus and Wolbachia,in two species of the *** whitefly complex,provisionally named as Asia II 3 and China *** sequence analyses revealed that Arsenophonus and Wolbachia retained genes responsible for the biosynthesis of B *** then conducted transcriptomic surveys of the bacteriomes in these two species of whiteflies together with that in another species named MED of this whitefly complex previously *** analyses indicated that several key genes in B vitamin syntheses from the three whitefly species were *** findings suggest that,similar to another secondary symbiont Hamiltonella,Arsenophonus and Wolbachia function in the nutrient provision of host *** phylogenetically distant species of symbionts are associated with their respective hosts,they have evolved and retained similar functions in biosynthesis of some B *** metabolic complementarity between whiteflies and symbionts represents an important feature of their coevolution.

关键词： symbiont B vitamins complement evolution whitefly

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Storm-Time Ionospheric Disturbances Monitored by GPS Beacon Measurements

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Wuhan University Journal of Natural Sciences 2001年第3期6卷 687-693页

作者： Pei Xiao hong, Yu sheng bing, Xu Ji sheng, Ma shu\|ying College of Electronic Information, Wuhan University, Wuhan 430072,China Wuhan University College of Electronic Information Wuhan China (GRID:grid.49470.3e) (ISNI:***)

The Total Electron Content (TEC) during three great storms, from April to August 2000, was collected by means of a GPS receiver located in Jingzhou (30.4° N, 112.2° E). The time-latitude-dependent features of ionospheric storms are identified using TEC difference images based on the deviations of TEC during storm relative to quiet time. The responses of ionospheric TEC to magnetic storms were analyzed. The results show that: 1) In middle and low latitude, ionospheric storms effects are more apparent in local day time than at night: 2) Ionospheric storm effects are more dominant near the hump of the equatorial anomaly region than in other regions of TEC measurements; 3) The positive effects during the main phase of ionospheric storm may be caused by electric fields in low latitude; 4) During the recovery period of ionospheric storm, the negative phase of storm may be due to the perturbation of the neutral gas composition.

关键词： global positioning system ionosphere total electron content magnetic storm ionospheric storm

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Neural Network Applications in Petroleum Exploration Based on Statistical Space Mapping

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Journal of China University of Mining and Technology 2005年第3期15卷 247-250页

作者： XU Zheng-guang HAO Qing-mei WANG shu-sheng WANG Zhi-liang School of Information Engineering University of Science and Technology Beijing Beijing 100083 China

In this paper, we propose the statistical space mapping thought and classify the seismic body space throughlithology space clustering combining to the actual application background of petroleum exploration. A new method ofstratum petroleum recognition based on neural network was set up through the foundation of the data mapping relationbetween log and seismic body. It can break a new path for recognition petroleum using both log and seismic data. Andthis method has been validated in the practical data analysis in Liaohe oil field.

关键词： petroleum exploration space mapping gray system theory lithology differentiation criterion seismic data log data

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Roles of supporting cells in the maintenance and regeneration of the damaged inner ear:A literature review

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Journal of Otology 2024年第4期19卷 234-240页

作者： Jing-Ying Guo Jun-Yi Xu shu-sheng Gong Guo-Peng Wang Department of Otolaryngology-Head and Neck Surgery Beijing Friendship HospitalCapital Medical UniversityBeijingChina Clinical Center for Hearing Loss Capital Medical UniversityBeijingChina

The inner ear sensory epithelium consists of two major types of cells:hair cells(HCs)and supporting cells(SCs).Critical functions of HCs in the perception of mechanical stimulation and mechanosensory transduction have long been *** are indispensable components of the sensory epithelia,and they maintain the structural integrity and ionic environment of the inner *** delicate inner ear epithelia sustain injuries(for example,due to ototoxic drugs or noise exposure),SCs respond immediately to serve as repairers of the epithelium and as adapters to become HC progenitors,aiming at morphological and functional recovery of the inner *** regenerative process is extensive in non-mammals,but is limited in the mammalian inner ear,especially in the mature *** review aimed to discuss the important roles of SCs in the repair of the mammalian inner ear.

关键词： epithelium injuries supporting

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Design and Synthesis of Glycosylated Aromatic Nitrogen Mustard Derivatives

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Chemical Research in Chinese Universities 2007年第3期23卷 300-302页

作者： LIU Tie-mei WANG shu-sheng ZHU Guang-ze LI Ming-yang ZHANG Li-ping School of Life Sciences Northeast Normal University Changchun 130024 P. R. China China-Japan Union Hospital Jilin Universty Changchun 130033 P. R. China School of Life Science Anhui University Hefei 230039 P. R. China College of Traditional Chingese Medicine in Changchun Changchun 130021 P. R. China

Antibody-directed enzyme prodrug therapy（ADEPT） is a new strategy for the treatment of cancer that has arisen in recent twenty years, the main merits of which are that it can improve the selectivity of anticancer drugs and reduce the side effects in remote tissue. In the present study, two prodrugs-glycosylated aromatic nitrogen mustard derivatives were synthesized. Glucose and lactose were converted into glycosyl donors-trichloroacetimidate; the obtained glycosyl donors were glycosylated with p-nitrophenol （ glycosyl donors） to form β-glucosyl p-nitrobenzene and β-lactosyl p-nitrobenzene that were protected by acetyl in a stereoselective manner; the two products were reduced by zinc dust and then treated with ethylene oxide, afforded two glycosylated nitrogen mustard derivatives that were protected by acetyl; the last step was to deacetylate and then afforded the two target compounds that could be used as prodrugs of ADEPT for further Anti-tumor research.

关键词： Glycosylation Nitrogen mustard derivative Prodrug ADEPT

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Proteomic analysis of heat stress resistance of cucumber leaves when grafted onto Momordica rootstock

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Horticulture Research 2018年第1期5卷 218-235页

作者： Ye Xu Yinghui Yuan Nanshan Du Yu Wang sheng shu Jin Sun Shirong Guo Key Laboratory of Southern Vegetable Crop Genetic Improvement in Ministry of Agriculture College of HorticultureNanjing Agricultural UniversityNanjingChina Department of Horticulture Henan Agricultural UniversityZhengzhouChina Suqian Academy of Protected Horticulture Nanjing Agricultural UniversitySuqianChina

Various biotic and abiotic stresses threaten the cultivation of future agricultural *** these stresses,heat stress is a major abiotic stress that substantially reduces agricultural *** strategies to enhance heat stress tolerance of crops have been developed,among which is ***,we show that Momordica-grafted cucumber scions have intrinsically enhanced chlorophyll content,leaf area,and net photosynthetic rate under heat stress compared to plants grafted onto cucumber *** investigate the mechanisms by which Momordica rootstock enhanced cucumber scions heat stress tolerance,comparative proteomic analysis of cucumber leaves in response to rootstock-grafting and/or heat stress was ***-seven differentially accumulated proteins involved in diverse biological processes were identified by two-dimensional electrophoresis(2-DE)in conjunction with matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry(MALDI-TOF/TOF MS).The following four main categories of proteins were involved:photosynthesis(42.8%),energy and metabolism(18.2%),defense response(14.3%),and protein and nucleic acid biosynthesis(11.7%).Proteomic analysis revealed that scions grafted onto Momordica rootstocks upregulated more proteins involved in photosynthesis compared to scions grafted onto cucumber rootstocks under heat stress and indicated enhanced photosynthetic capacity when seedlings were exposed to heat ***,the expression of photosynthesis-related genes in plants grafted onto Momordica rootstocks significantly increased in response to heat *** addition,increased high-temperature tolerance of plants grafted onto Momordica rootstock was associated with the accumulation of ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)and oxygen-evolving enhancer protein 1(OEE1).Taken together,the data indicated that Momordica rootstock might alleviate growth inhibition caused by heat stress by improving ph

关键词： crops stress resistance

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The Translation Invariant Contourlet-like Transform for Image Denoising

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自动化学报 2009年第5期35卷 505-508页

作者： LIAN Qiu-sheng CHEN shu-Zhen Institute of Information Science and Technology Yanshan Uni-versity Qinhuangdao 066004 P.R. China

The contourlet transform with anisotropy and directionality is a new extension to the wavelet transform. Because of its filter bank structure, the contourlet transform is not translation-invariant. In this paper, we propose the translation-invariant contourlet-like transform (TICLT) with lower redundancy than both the nonsubsampled contourlet transform (NSCT) and the translation invariant contourlet transform (TICT). The TICLT is constructed by combining the translation invariant Laplacian pyramid and undecimated directional filter banks. The undecimated directional filter banks, which satisfy the perfect reconstruction condition, are designed by the mapping approach using one-dimensional fractional splines orthogonal filter banks as prototype filters. We evaluate the performance of the TICLT in image denoising. Some comparisons with the state-of-the-art denoising methods are given to illustrate the potential of the TICLT.

关键词：滤波技术滤波器图像降噪

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Immunoglobulin G4-related kidney disease involving the renal pelvis and perirenal fat:A case report

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World Journal of Clinical Cases 2022年第8期10卷 2510-2515页

作者： Jun-Wei He Qian-Ming Zou Jun Pan shu-sheng Wang Song-Tao Xiang Department of Urology the Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510000Guangdong ProvinceChina

BACKGROUND Immunoglobulin(Ig)G4-related disease(Ig G4-RD)is an autoimmune disease associated with chronic and progressive inflammation and *** is difficult to differentiate Ig G4-RD involving the kidney from infectious diseases and malignancy on *** SUMMARY We report the case of a 51-year-old Chinese man whose abdominal computed tomography scan showed diffuse bilateral enlargement of the kidneys and perirenal fat,thickening of the renal pelvic walls,and hydronephrosis of the right *** laboratory test results showed a serum creatinine level of 464μmol/*** patient was diagnosed with acute renal failure and was started on intermittent *** tests revealed high serum Ig G4 levels(20.8 g/L)and an enlarged right submaxillary lymph *** and histopathological examination of the enlarged node led to the diagnosis of Ig *** corticosteroid therapy,his serum creatinine level quickly decreased to near normal *** Ig G4-RD affecting the renal pelvis or perirenal fat is rare,with atypical imaging *** consultation is critical for accurate diagnosis and treatment of this *** cases should undergo biopsy to avoid misdiagnosis.

关键词： Immunoglobulin G4-related disease Renal pelvic Perirenal fat Case report

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