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The Mining of Citrus EST-SNP and Its Application in Cultivar Discrimination

Agricultural Sciences in China 2010年第2期9卷 179-190页

作者： JIANG Dong YE Qing-liang WANG Fu-sheng cao li Citrus Research Institute Chinese Academy of Agricultural Sciences Chongqing 400712 P.R.China

Single nucleotide polymorphisms （SNPs） are the most abundant sequence variations found in plant genomes and are widely used as molecular genetic markers in cultivar identification and genetic diversity studies. The objective of this study was to identify SNP markers useful for discrimination of citrus cultivars, since large numbers of expressed sequence tags （ESTs） of sweet orange are available from the National Center for Biotechnology Information （NCBI）. We now have the opportunity to discover SNP markers suitable for determining the haplotypes with which to distinguish very closely related cultivars and to assess genetic diversity within or between related species of citrus. SNPs and small insertions/deletions （Indels） from ESTs of sweet orange and satsuma were identified by the in silico SNP discovery strategy. 55 296 EST sequences of sweet orange and 2 575 of satsuma retrieved from the NCBI repository were mined for potential SNPs. Cleaved amplified polymorphic sequences （CAPS） and sequencing approaches were used to validate putative SNPs in a sample of 30 citrus accessions. A total of 3 348 putative SNPs were identified based on the abundance of sequences and haplotype cosegregation. Of these 3 348 SNPs, the transitions, transversions and Indels ratios were 47.9, 36.1 and 16.0%, respectively. The SNPs occurred on average at a frequency of 1 per 164 bp in the coding region of citrus. 14 SNPs were randomly selected and genotyped according to 30 citrus accessions including 23 accessions of sweet orange; 11 SNPs displayed polymorphism with an average polymorphism information content （PIC） of 0.20 among 30 citrus accessions. The genetic diversity present in sweet orange was low, so the 14 SNP markers failed to discriminate different cultivars of sweet orange, but they did succeed in distinguishing accessions of inter-species of citrus. In this study, SNPs were mined from EST sequences of sweet orange and satsuma, which displayed potential capability as molecular ma

关键词： citrus single nucleotide polymorphisms (SNPs) EST-SNP cleaved amplified polymorphic sequences (CAPS)

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Synthesis and Biological Activities of N-Phosphoryl Branched Peptides

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Chinese Chemical Letters 2003年第4期14卷 343-346页

作者： sheng li cao Yu Yang JIANG Yu Ping FENG Yan ling NIU Yu Fen ZHAO Key Laboratory of Bioorganic Phosphorus Chemistry Ministry of EducationDepartment of ChemistryTsinghua UniversityBeijing 100084 Department of Chemistry Capital Normal UniversityBeijing 100037

A series of N-phosphoryl branched peptides were synthesized by coupling of various N-phosphoryl amino acids to L-Lysine methyl ester, and their structures were confirmed by 31P NMR, 1H NMR, MS and elemental analysis. The results of cell biological tests indicated that compound 1d and 1e obviously inhibited the growth of both K562 and A2780 cells.

关键词： N-Phosphoryl branched peptide synthesis biological activity apoptosis.

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Sequential Inverse Optimal Control of Discrete-Time Systems

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IEEE/CAA Journal of Automatica Sinica 2024年第3期11卷 608-621页

作者： sheng cao Zhiwei Luo Changqin Quan the Graduate School of System Informatics Kobe University Kobe 657-8501Japan

This paper presents a novel sequential inverse optimal control(SIOC)method for discrete-time systems,which calculates the unknown weight vectors of the cost function in real time using the input and output of an optimally controlled discrete-time *** proposed method overcomes the limitations of previous approaches by eliminating the need for the invertible Jacobian *** calculates the possible-solution spaces and their intersections sequentially until the dimension of the intersection space decreases to *** remaining one-dimensional vector of the possible-solution space’s intersection represents the SIOC *** paper presents clear conditions for convergence and addresses the issue of noisy data by clarifying the conditions for the singular values of the matrices that relate to the possible-solution *** effectiveness of the proposed method is demonstrated through simulation results.

关键词： Inverse optimal control promised calculation step sequential calculation

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Toward high performance microwave absorber by implanting La_(0.8)CoO_(3)nanoparticles on rGO

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Journal of Materials Science & Technology 2024年第7期174卷 176-187页

作者： li Chang Yu-Ze Wang Xin-Ci Zhang lin li Hua-Zhang Zhai Mao-sheng cao Key Laboratory for Photonic and Electronic Bandgap Materials Ministry of EducationSchool of Physics&Electronic EngineeringHarbin Normal UniversityHarbin 150025China School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China

The advent of“intelligent era”brings our life more convenience,but the electromagnetic radiation sur-rounding us not only greatly threatens human health,also makes information leakage and hidden trouble to national defense ***,it is very urgent to develop novel electromagnetic wave absorption materials with lightweight,strong absorption,tunable absorption frequency and broad band ***,a novel electromagnetic wave absorber is obtained by constructing La_(0.8)CoO_(3)-rGO nanocompos-ite,where La_(0.8)CoO_(3)nanoparticles are anchored on graphene nanosheets by the electrostatic interaction between GO and La_(0.8)CoO_(3).The effect of hybridization ratio of La_(0.8)CoO_(3)and rGO on microwave ab-sorption properties is carefully *** optimal reflection loss of La_(0.8)CoO_(3)-rGO nanocomposite can reach-62.34 dB with the maximum effective bandwidth of 6.08 GHz,presenting 48.78%and 245.45%increment compared to bare La_(0.8)CoO_(3)nanoparticles,*** effective absorption bandwidth covers a broad electromagnetic wave absorption band from Ku band to the C band by tailoring thickness of the absorbers from 2.4 mm to 4.4 *** fascinating electromagnetic wave absorption performance is attributed to the synergy effect of La_(0.8)CoO_(3)and rGO,which integrates magnetic and dielectric loss caused by resonance,conductance,relaxation,and scattering *** result confirms that La_(0.8)CoO_(3)-rGO nanocomposite is potential candidates toward high-efficiency microwave absorbers and provides a valuable pathway for designing high-performance microwave attenuation materials in the future.

关键词： Perovskite La_(0.8)CoO_(3)nanoparticles Graphene Nanocomposite Microwave absorbing Broad band absorption

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Improving antioxidant activities of water-soluble lignin-carbohydrate complex isolated from wheat stalk through prolonging ball-milling pretreatment and homogeneous extraction

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Journal of Bioresources and Bioproducts 2024年第1期9卷 113-125页

作者： Di Xie Zhulan liu Yunfeng cao sheng-I Yang Chen Su Mi li Jiangsu Co-innovation Center for Efficient Processing and Utilization of Forest Products Nanjing Forestry UniversityNanjing 210037China International Innovation Center for Forest Chemicals and Materials Nanjing Forestry UniversityNanjing 210037China Center for Renewable Carbon School of Natural ResourcesUniversity of TennesseeKnoxvilleTN 37996USA Shandong Huatai Paper Co. Ltd.Dongying 257000China School of Natural Resources University of TennesseeKnoxvilleTN 37996USA Key Lab of Biomass Energy and Material Institute of Chemical Industry of Forest ProductsChinese Academy of ForestryJiangsu ProvinceNanjing 210042China

Water-soluble lignin-carbohydrate complex(LCC)rich in polysaccharides exhibits benign in vitro antioxidant activities and distinguishes high biocompatibility from lignin-rich LCC and ***,the antioxidant activity of water-soluble LCCs remains to be improved and its structure-antioxidant relationship is still ***,structurally diversified water-soluble LCCs were isolated under different ball-milling pretreatment durations(4,6,8 h),extraction pathways(ho-mogeneous and heterogeneous),and isolation routines(water extracts and residues after water extraction).Their structures were characterized by wet chemistry,chromatography and *** activities were evaluated by ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl radicals scavenging rate(RDPPH).Results show that altering ball-milling duration and isolation procedures cause varied structures and antioxidant activities of the water-soluble ***,prolonging ball-milling duration to 8 hours and homogeneous extrac-tion can enhance their antioxidant activity through releasing more phenolic structures and pro-moting the extraction of high-molecular-weight LCCs via reducing mass-transfer resistance,*** a result,the RDPPH of water-soluble LCCs reaches up to 97.35%,which is associated with the arabinan content with statistical significance(P<0.05).This study provides new insights into the structure-antioxidation relationship of herbaceous LCC as potential antioxidants.

关键词： LCC Homogeneous extraction Ball-milling Structure-antioxidation relationship Wheat straw

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H∞ Robustness for Distributed Control in Autonomous Microgrids Considering Cyber Disturbances

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CSEE Journal of Power and Energy Systems 2024年第2期10卷 696-706页

作者： Ge cao Guannan Lou Wei Gu lina sheng School of Electrical Engineering Southeast UniversityNanjing 210096China

The physical connections and logical relationships between microgrids and communication networks allow microgrids to develop into typical cyber-physical systems(CPSs).With the extensive use of open communication mechanisms,the impact of cyber disturbances in public communication networks cannot be *** this paper,a parameter optimal method for a distributed secondary controller based on the robust control theory and consensus algorithm is presented to enhance the robustness of a secondary control system under data disturbance,parameter perturbation,and time ***,a distributed secondary control strategy of microgrids is demonstrated that coordinates frequency and voltage restoration and power ***,considering the impact of cyber events on the secondary control,a distributed robust controller gain design method is proposed to satisfy the H∞ performance *** solution of the distributed robust control is transformed into a linear matrix in equation problem and latency margin is simultaneously ***,a test microgrid CPS is simulated with and without time delay to investigate the impact of cyber events on system operational states and the effectiveness and robustness of the proposed method.

关键词： Cyber disturbance cyber-physical lsystem distributed control microgrid robust H∞control

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Curve Classification Based onMean-Variance Feature Weighting and Its Application

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Computers, Materials & Continua 2024年第5期79卷 2465-2480页

作者： Zewen Zhang sheng Zhou Chunzheng cao School of Mathematics and Statistics Nanjing University of Information Science and TechnologyNanjing210044China Center for Applied Mathematics of Jiangsu Province Nanjing University of Information Science and TechnologyNanjing210044China

The classification of functional data has drawn much attention in recent *** main challenge is representing infinite-dimensional functional data by finite-dimensional features while utilizing those features to achieve better classification *** this paper,we propose a mean-variance-based(MV)feature weighting method for classifying functional data or functional *** the feature extraction stage,each sample curve is approximated by B-splines to transfer features to the coefficients of the spline *** that,a feature weighting approach based on statistical principles is introduced by comprehensively considering the between-class differences and within-class variations of the *** also introduce a scaling parameter to adjust the gap between the weights of *** new feature weighting approach can adaptively enhance noteworthy local features while mitigating the impact of confusing *** algorithms for feature weighted K-nearest neighbor and support vector machine classifiers are both ***,the new approach can be well integrated into existing functional data classifiers,such as the generalized functional linear model and functional linear discriminant analysis,resulting in a more accurate *** performance of the mean-variance-based classifiers is evaluated by simulation studies and real *** results show that the newfeatureweighting approach significantly improves the classification accuracy for complex functional data.

关键词： Functional data analysis classification feature weighting B-splines

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T-CELL RESPONSE OF ADVANCED AIDS PATIENTS AFTER HIGHLY ACTIVE ANTIRETROVIRAL THERAPY

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Chinese Medical Sciences Journal 2005年第4期20卷 223-225,页

作者： Ai-xia Wang Tai-sheng li Yun-zhen cao Yang Han Zhi-feng Qiu Jing Xie Department of Medicine Infectious Diseases Section Peking Union Medical College Hospital Chiness Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730

Objective To investigate the response on late stage Chinese AIDS patients after highly active antiretroviral therapy （HAART）. Methods From October 2002 to March 2004, 20 cases of late stage Chinese AIDS patients were selected to participate in this opened and randomised study, we purposely chose those with CD4＋ T cell counts 〈 100/mm^3. All of them had one or two opportunistic infections and none had been treated with anti-HIV drugs. All patients were tested with CD4＋（naive CD4＋ T cell defined by CD45RA＋ and CD62L＋, memory CD4＋ T cell defined by CD45RA-）, CD8＋ T cell, plasma HIV viral load, and clinical manifestations on before, during, and after HAART （5 different regimes） on 1, 3, 6, 9, and 12 months. Before HAART mean CD4＋ T cell counts were 32 ± 31 （range 2-91）/mm^3, and plasma HIV viral load were 5.07 ± 0.85（range 2.04-5.70） log copies/mL. In 1 month＇s time patients treated with HAAT had mean CD4＋ and CD8＋ T cell counts increasing rapidly. After 1 month the increasing speed turned to slow down, but HIV viral load decreased predominantly within the first 3 months. The major part of increasing CD4＋ T cells were memory CD4＋ T cells, as for naive CD4＋ T cells increasing low and slow. Clinical symptoms and signs improved, and opportunistic infections reduced. The quality of life will be far much better than before. Each patient was followed for 12 months, and had finished 12 months＇ HAAT. Conclusion This is the first report in China that late stage Chinese AIDS patients after HAART could have their immune reconstitution. The regular pattern is similar to what had been reported in Western countries and also in China. So it is worth to treat late stage Chinese AIDS patients with HAAT.

关键词： acquired immune deficiency syndrome highly active antiretroviral therapy immune reconstitution

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Volume-averaged modeling of multiphase solidification with equiaxed crystal sedimentation in a steel ingot

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China Foundry 2024年第3期21卷 229-238页

作者： Xiao-lei Zhu Shuang cao Rui Guan Ji Yang Zhe Ning Xin-gang Ai sheng-li li Xin-cheng Miao The key Laboratory of Material Forming and Structure Property Control Anshan 114051LiaoningChina State Key Laboratory of Metal Material for Marine Equipment and Application Anshan 114009LiaoningChina School of Materials and Metallurgy University of Science and Technology LiaoningAnshan 114051LiaoningChina

Macrosegregation is a critical factor that limits the mechanical properties of *** impact of equiaxed crystal sedimentation on macrosegregation has been extensively studied,as it plays a significant role in determining the distribution of alloying elements and impurities within a *** improve macrosegregation in steel connecting shafts,a multiphase solidification model that couples melt flow,heat transfer,microstructure evolution,and solute transport was established based on the volume-averaged Eulerian-Eulerian *** this model,the effects of liquid phase,equiaxed crystals,columnar dendrites,and columnar-to-equiaxed transition(CET)during solidification and evolution of microstructure can be considered *** sedimentation of equiaxed crystals contributes to negative macrosegregation,where regions between columnar dendrites and equiaxed crystals undergo significant A-type positive macrosegregation due to the ***,noticeable positive macrosegregation occurs in the area of final solidification in the *** improvement in macrosegregation is beneficial for enhancing the mechanical properties of connecting *** mitigate the thermal convection of molten steel resulting from excessive superheating,reducing the superheating during casting without employing external fields or altering the design of the ingot mold is indeed an effective approach to control macrosegregation.

关键词： ingot casting multiphase solidification model equiaxed crystal sedimentation microstructure macrosegregation

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Energy modeling and optimization of building condenser water systems with all-variable speed pumps and tower fans:A case study

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Building Simulation 2024年第7期17卷 1085-1111页

作者： Yong cao Chuang Wang sheng Wang Xiao Fu Xinguo Ming School of Mechanical Engineering Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Air-Conditioning Equipment and System Energy Conservation Zhuhai 519070China GREE Electric Appliances Inc.of ZhuhaiZhuhai 519070China School of Environment and Energy Engineering Beijing University of Civil Engineering and ArchitectureBeijing 100044China Beijing Advanced Innovation Centerfor Future Urban Design Beijing University of Civil Engineering and ArchitectureBeijing 100044China

The emergence of building condenser water systems with all-variable speed pumps and tower fans allows for increased efficiency and flexibility of chiller plants in partial load operation but also increases the control complexity of condenser water *** study aims to develop an integrated modeling technique for evaluating and optimizing the energy performance of such a condenser water *** proposed system model is based on the semi-physical semi-empirical chiller,pump,and cooling tower models,with capabilities of fully considering the hydraulic and thermal interactions in the condenser water loop,being solved analytically and much faster than iterative solvers and supporting the explicit optimization of the pump and tower fan frequency.A mathematical approach,based on the system model and constrained optimization technique,is subsequently established to evaluate the energy performance of a typical dual setpoint-based variable speed strategy and find its energy-saving potential and most efficient operation by jointly optimizing pumps and tower *** all-variable speed chiller plant from Wuhan,China,is used for a case study to validate the system model’s accuracy and explore its *** results showed that the system model can accurately simulate the condenser water system’s performance under various operating *** optimizing the frequencies of pumps and tower fans,the total system energy consumption can be reduced by 12%–13%compared to the fixed dual setpoint-based strategy with range and approach setpoints of 4℃and 2℃.In contrast,the energy-saving potential of optimizing the cooling tower sequencing is insignificant.A simple joint speed control method for optimizing the pumps and tower fans emerged,i.e.,the optimal pump and fan frequency are linearly correlated(if both are non-extremes)and depend on the chiller part load ratio only,irrespective of the ambient wet-bulb temperature and chilled water supply *** was also foun

关键词： all-variable speed chiller plant model-based optimization hydraulic and thermal coupling analytical solution dual setpoint-based variable speed control optimal joint speed control

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