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Quantitative estimation of enzymatic released specific oligosaccharides from Hericium erinaceus polysaccharides using CE-liF

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Journal of Pharmaceutical Analysis 2023年第2期13卷 201-208页

作者： Yong Deng Jing Zhao shaoping li State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR999078China Joint Laboratory of Chinese Herbal Glycoengineering and Testing Technology University of MacaoMacao SAR999078China Macao Centre for Testing of Chineese Medicine University of MacaoMacao SAR999078China

Polysaccharides exhibit multiple pharmacological activities which are closely related to their structural features.Therefore,quantitatively quality control of polysaccharides based on their chemical charac-teristics is important for their application in biomedical and functional food sciences.However,poly-saccharides are mixed macromolecular compounds that are difficult to isolate and lack standards,making them challenging to quantify directly.In this study,we proposed an improved saccharide mapping method based on the release of specific oligosaccharides for the assessment of Hericium eri-naceus polysaccharides from laboratory cultured and different regions of China.Briefly,a polysaccharide from H.erinaceus was digested by b-(1-3)-glucanase,and the released specific oligosaccharides were labeled with 8-aminopyrene-1,3,6-trisulfonic-acid(APTS)and separated by using micellar electrokinetic chromatography(MEKC)coupled with laser induced fluorescence(liF),and quantitatively estimated.MEKC presented higher resolution compared to polysaccharide analysis using carbohydrate gel elec-trophoresis(PACE),and provided great peak capacity between oligosaccharides with polymerization degree of 2(DP2)and polymerization degree of 6(DP6)in a dextran ladder separation.The results of high performance size exclusion chromatography coupled with multi-angle laser light scattering and refractive index detector(HPSEC-MALLS-RI)showed that 12 h was sufficient for complete digestion of polysaccharides from H.erinaceus.Laminaritriose(DP3)was used as an internal standard for quantifi-cation of all the oligosaccharides.The calibration curve for DP3 showed a good linear regression(R2>0.9988).The limit of detection(LOD)and limit of quantification(LOQ)values were 0.05 mg/mL and 0.2 mg/mL,respectively.The recovery for DP3 was 87.32(±0.03)%in the three independent injections.To sum up,this proposed method is helpful for improving the quality control of polysaccharides from H.erinaceus as well as other materials.

关键词： Saccharide mapping Hericium erinaceus Polysaccharide CE-liF PACE

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Analysis of Cordyceps by multi-column liquid chromatography

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Acta Pharmaceutica Sinica B 2017年第2期7卷 202-207页

作者： Zhengming Qian shaoping li Institute of Chinese Medical Sciences University of MacaoMacaoChina Key Laboratory of State Administration of Traditional Chinese Medicine Sunshine Lake Pharma Co.Ltd.Dongguan 523850China

Cordyceps is a famous traditional Chinese medicine(TCM) that has been used in China for hundreds of years.In the present study a multi-column liquid chromatography(MC-LC) system was developed for the qualitative analysis of macromolecules and micromolecules in Cordyceps.The MC-LC system includes a size exclusion pre-column,a size exclusion column(SEC) and a reversed phase column(RP) which were controlled by column-switching valves.The sample was separated by the size exclusion pre-column into two fractions(macromolecules and micromolecules).These fractions were further separated on SEC and RP columns,respectively.A diode array detector(DAD) and a mass spectrometer(MS) were used to detect the components.This MC-LC method was utilized for analysis of Cordyceps samples.Two macromolecular peaks and 15 micromolecular peaks were found in Cordyceps,and 11 of the micromolecular peaks were identified as adenosine-5'-monophosphate(AMP),phenylalanine,uridine,hypoxanthine,inosine,guanine,guanosine,deoxyadenosine-5'-monophosphate(dAMP),adenosine,adenine and cordycepin(or its isomer).This method is useful for quality control of Cordyceps.

关键词： Cordyceps Macromolecules Micromolecules Multi-column Size exclusion column

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Current-Induced Magnetic Switching in an L1_(0) FePt Single Layer with Large Perpendicular Anisotropy Through Spin–Orbit Torque

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Engineering 2022年第5期8卷 55-61页

作者： Kaifeng Dong Chao Sun Laizhe Zhu Yiyi Jiao Ying Tao Xin Hu Ruofan li Shuai Zhang Zhe Guo Shijiang Luo Xiaofei Yang shaoping li Long You School of Automation China University of GeosciencesWuhan 430074China Hubei Key Laboratory of Advanced Control and Intelligent Automation for Complex Systems China University of GeosciencesWuhan 430074China School of Optical and Electronic Information Huazhong University of Science and TechnologyWuhan 430074China China Resources Microelectronics limited Shanghai 200072China Shenzhen Huazhong University of Science and Technology Research Institute Shenzhen 518000China Wuhan National High Magnetic Field Center Huazhong University of Science and TechnologyWuhan 430074China

In this study,current-induced partial magnetization-based switching was realized through the spin–orbit torque(SOT)in single-layer L1_(0) FePt with a perpendicular anisotropy(K_(u⊥))of 1.19×10^(7) erg·cm^(-3)(1 erg·cm^(-3)=0.1 J·m^(-3)),and its corresponding SOT efficiency(βDL)was 8×10^(-6) Oe·(A·cm^(-2))^(-1)(1 Oe=79.57747 A·m^(-1)),which is several times higher than that of the traditional Ta/CoFeB/MgO structure reported in past work.The SOT in the FePt films originated from the structural inversion asymmetry in the FePt films since the dislocations and defects were inhomogeneously distributed within the samples.Furthermore,the FePt grown on MgO with a granular structure had a larger effective SOT field and effi-ciency than that grown on SrTiO_(3)(STO)with a continuous structure.The SOT efficiency was found to be considerably dependent on not only the sputtering temperature-induced chemical ordering but also the lattice mismatch-induced evolution of the microstructure.Our findings can provide a useful means of efficiently electrically controlling a magnetic bit that is highly thermally stable via SOT.

关键词： L1_(0)FePt SOT Inversion asymmetry Magnetic switching Perpendicular anisotropy

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Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China

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Journal of Pharmaceutical Analysis 2021年第3期11卷 284-291页

作者： Yong Deng Cunwu Chen lingxiao Chen Bangxing Han shaoping li Jing Zhao State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacaoChina Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources West Anhui UniversityLu'anAnhuiChina

Due to the extensive use of xylooligosaccharides(XOS)as functional food ingredients,many inferior goods and even adulterants are generally found in the market,which may pose a health hazard to certain populations.Chromatography method such as high-performance liquid chromatography(HPLC)and high-performance thin-layer chromatography(HPTLC)is traditionally applied for the quality analysis of XOS.However,it is time consuming due to the prolonged separation and pre-or post-derivatization procedure.In this study,a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry(MALDI-TOF-MS)was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China.The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate,at least 6 times faster than that by the traditional HPTLC chromatography method.In addition,MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of m/z,which is hardly separated using HPTLC.The results showed that XOS were present only in samples XY01-XY11,samples XY12-XY14 only consisted of hex oligosaccharides,and samples XY15-XY22 were free of oligosaccharides.These indicate that the quality consistency of XOS products in the China market was poor,which should be carefully investigated.

关键词： Xylooligosaccharides Saccharide mapping MALDI-MS Quality control

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Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology

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Journal of Pharmaceutical Analysis 2016年第6期6卷 382-388页

作者： Wei Xiong Xianqiang Chen Guangping Lv Dejun Hu Jing Zhao shaoping li State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao SAR

In this work,a fast and efficient microwave-assisted extraction(MAE) method was developed to extract main bioactive alkaloids from lotus plumue.To optimize MAE conditions,three main factors were selected using univariate approach experiments,and then central composite design(CCD).The optimal extraction conditions were as follows:methanol concentration of 65%,microwave power of 200 W,and extraction time of 260 s.A high performance liquid chromatography–diode array detector(HPLC–DAD) method was established to quantitatively analyze these phytochemicals in different lotus plumule samples and in different part of lotus.Chromatographic separation was carried out on an Agilent Zorbax Extend-C_(18) column(4.6 mm×150 mm,3.5 μm).Gradient elution was applied with the mobile phase constituted with 0.1% triethylamine in water(A)and acetonitrile(B):40%-70% B at 0-8 min,70%-100% B at 8–9 min,100% B for 2 min,and then equilibrated with 40% B for 2 min.

关键词： Lotus plumule Functional food Microwave-assisted extraction Alkaloids

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数字化发展、乡村建设与农村节能减排

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农林经济管理学报 2024年第2期23卷 206-215页

作者：张恒硕李绍萍钱学胜东北石油大学经济管理学院黑龙江大庆163318 复旦大学管理学院上海200443

基于2011—2020年中国30个省份的面板数据,采用固定效应模型和面板门槛模型,实证分析数字化发展对农村节能减排的影响及门槛效应,并从乡村建设视角揭示乡村宜居与宜业建设的绿色渠道作用。结果表明:数字化发展能够显著抑制农村能源强度... 详细信息

基于2011—2020年中国30个省份的面板数据,采用固定效应模型和面板门槛模型,实证分析数字化发展对农村节能减排的影响及门槛效应,并从乡村建设视角揭示乡村宜居与宜业建设的绿色渠道作用。结果表明:数字化发展能够显著抑制农村能源强度和碳强度的上涨,有效提升农村节能减排成效;异质性分析表明,数字化发展在农业生产方面发挥的降能效应更为明显,在农村生活方面发挥的减碳效应更为突出;进一步分析表明,数字化发展对农村节能减排的作用存在倒“U”型的非线性影响特征,数字化发展能够推动乡村宜居建设和乡村宜业建设,进而为农村节能减排创造有利条件。据此,建议加快乡村数字基础设施建设,充分发挥数字化发展的环境红利;提升乡村数字渗透能力,培育农村绿色转型发展的数字环境;深入推进数字乡村建设,增强数字化在乡村宜居宜业建设的赋能效果。

关键词：数字化发展乡村建设节能减排

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木质纤维素气凝胶制备及阻燃改性

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化学进展 2024年第4期36卷 586-600页

作者：王爽张鑫孙苗段红娟张海军李少平武汉科技大学省部共建耐火材料与冶金国家重点实验室武汉430081 湖北三峡实验室宜昌443007

木质纤维素气凝胶具有密度低、孔隙率高、导热系数低等优异性能,被广泛应用于保温隔热、吸附、催化、电磁屏蔽和生物医学等领域。同时木质纤维素也是一种生物基材料,具有绿色、无污染、可再生和可持续的特点。本文首先综述了木材基纤维... 详细信息

木质纤维素气凝胶具有密度低、孔隙率高、导热系数低等优异性能,被广泛应用于保温隔热、吸附、催化、电磁屏蔽和生物医学等领域。同时木质纤维素也是一种生物基材料,具有绿色、无污染、可再生和可持续的特点。本文首先综述了木材基纤维素和农作物废弃物基纤维素气凝胶的最新研究进展,然后综述了冷冻干燥、超临界干燥和常压干燥制备木质纤维素气凝胶的研究现状。其次,针对木质纤维素气凝胶普遍存在的易燃问题,详述了提高木质纤维素气凝胶阻燃性能的常用方法。最后,提出了木质纤维素气凝胶制备及性能方面存在的主要问题,并对该领域未来的发展方向进行了展望。

关键词：气凝胶木质纤维素干燥技术阻燃性能

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Effect of Migration on Children's Self-esteem in Rural China

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China & World Economy 2014年第4期22卷 83-101页

作者： Xuefeng Zhan shaoping li Chengfang liu linxiu Zhang Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences University of Chinese Academy of Sciences Center for Chinese Agricultural Policy Institute of Geographical Sciences and Natural Resource ResearchChinese Academy of Sciences

China＇s rapid economic growth has been facilitated by its large volume of rural to urban migration. China ＇s projected future development, especially increasing urbanization, implies that such migration will further intensify. However, migration does not come without cost. There are concerns about the potential negative impacts of migration on children＇s care, education, and, in particular, the self-esteem of children left behind in villages where one or both parents have out-migrated to cities. In this paper, we employ unique survey data collected from Shaanxi Province, where more than 4700 ninth grade students from 36 rural junior high schools in five counties were surveyed in late 2011. The results show that having both parents migrate into cities significantly reduced children ＂s self-esteem. The effects are also gender sensitive. Girls that had a father or both parents who had out-migrated were inclined to have lower self-esteem than boys. Moreover, our study findings indicate that parental migration decreased children ＂s self-esteem more for individuals with initial low self-esteem.

关键词： Key words： children left-behind migration rural China self-esteem

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液体活检在肿瘤诊疗全程管理中的现状及展望

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中国临床研究 2024年第6期37卷 959-964页

作者：李少平王迪杨若巍杨珊珊余蕾南京大学医学院附属鼓楼医院健康管理中心江苏南京210008 南京世和基因生物技术股份有限公司江苏南京210032

有效的检测方法能够在早期发现恶性肿瘤迹象,提高患者生存率。液体活检,作为一项新兴的无创技术,通过检测体液中的生物标志物,为恶性肿瘤提供全方位的信息,从而指导更加精准的治疗。液体活检的主要检测指标包括循环肿瘤DNA、循环肿瘤细... 详细信息

有效的检测方法能够在早期发现恶性肿瘤迹象,提高患者生存率。液体活检,作为一项新兴的无创技术,通过检测体液中的生物标志物,为恶性肿瘤提供全方位的信息,从而指导更加精准的治疗。液体活检的主要检测指标包括循环肿瘤DNA、循环肿瘤细胞、微小RNA和外泌体等。通过使用不同的技术手段检测这些生物标志物,可以为不同发展阶段的肿瘤提供治疗方向,延长患者的生存期,提高生活质量。相较于传统组织检测,液体活检作为一种非侵入式的手段,具有无创性,克服肿瘤异质性及可以实时监测肿瘤情况等优势。随着技术的不断发展,液体活检在早期、中期和晚期恶性肿瘤治疗中都具有重要的临床指导价值,包括指导肿瘤治疗、研究抗药性机制、预测患者预后、监测复发以及协助早期筛查和诊断。本文将综述当前液体活检技术的发展状况,深入分析液体活检所使用的生物标志物,探讨其在肿瘤诊疗全程管理中的研究进展。

关键词：肿瘤液体活检全程管理外泌体循环肿瘤细胞微小残留病灶甲基化片段组学微小RNA

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Clinical features of 162 fatal cases of COVID-19:a multi-center retrospective study

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Emergency and Critical Care Medicine 2022年第3期2卷 109-115页

作者： Xianlong Zhou Guoyong Ding Qing Fang Jun Guo Luyu Yang Ping Wang Shou-Zhi Fu Ang li Jian Xia Jiangtao Yu Jianyou Xia Min Ma Zhuanzhuan Hu Lei Huang Ruining liu Cheng Jiang shaoping li Mingxia Yu Xizhu Xu Yan Zhao Quan Hu Weijia Xing Zhigang Zhao Emergency Center Zhongnan Hospital of Wuhan UniversityWuhanHubeiChina School of Public Health Shandong First Medical University and Shandong Academy of Medical SciencesTai’anShandongChina Emergency Department General Hospital of Central Theater Command of PLAWuhanHubeiChina Intensive Care Unit Huazhong University of Science and Technology Union Jiangbei HospitalWuhanHubeiChina Intensive Care Unit Wuhan Third HospitalWuhanHubeiChina Department of Urology General Hospital of Central Theater Command of PLAWuhanHubeiChina Department of Clinical Laboratory Zhongnan Hospital of Wuhan UniversityWuhanHubeiChina Hubei Clinical Research Center for Emergency and Resuscitation Zhongnan Hospital of Wuhan UniversityWuhanHubeiChina Intensive Care Unit The First People’s Hospital of Jiangxia DistrictWuhanHubeiChina

Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal cases of COVID-19 from 5 hospitals in Wuhan between December 30,2019 and March 12,2020.Methods:The demographic data,signs and symptoms,clinical course,comorbidities,laboratory findings,computed tomographic(CT)scans,treatments,and complications of the patients with fatal cases were retrieved from electronic medical records.Results:The median patient age was 69.5(interquartile range:63.0–77.25)years,and 80%of the patients were over 61 years.A total of 112(69.1%)patients were men.Hypertension(45.1%)was the most common comorbidity,while 59(36.4%)patients had no comorbidity.At admission,131(81.9%)patients had severe or critical COVID-19,whereas 39(18.1%)patients with hypertension or chronic lung disease had moderate COVID-19.In total,126(77.8%)patients received antiviral treatment,while 132(81.5%)patients received glucocorticoid treatment.A total of 116(71.6%)patients were admitted to the intensive care unit(ICU),and 137(85.1%)patients received mechanical ventilation.Most patients received mechanical ventilation before ICU admission.Approximately 93.2%of the patients developed respiratory failure or acute respiratory distress syndrome.There were no significant differences in the inhospital survival time among the hospitals(P=0.14).Conclusion:Young patients with moderate COVID-19 without comorbidity at admission could also develop fatal outcomes.The in-hospital survival time of the fatal cases was similar among the hospitals of different levels in Wuhan.

关键词： Clinical features Coronavirus disease 2019 Fatal cases Survival time

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