Optimization of microwave-assisted extraction of bioactive alkaloids from lotus plumule using response surface methodology

作     者：Wei Xiong Xianqiang Chen Guangping Lv Dejun Hu Jing Zhao Shaoping Li 

作者机构：State Key Laboratory of Quality Research in Chinese MedicineInstitute of Chinese Medical SciencesUniversity of MacaoMacao SAR 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2016年第6卷第6期

页      面：382-388页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：partially supported by grants from the Science and Technology Development Fund of Macao(FDCT059/2011/A3) the University of Macao(MYRG085 to Jing Zhao and MYRG201400041 to LSP,respectively) 

主　　题：Lotus plumule Functional food Microwave-assisted extraction Alkaloids 

摘      要：In this work,a fast and efficient microwave-assisted extraction(MAE) method was developed to extract main bioactive alkaloids from lotus *** optimize MAE conditions,three main factors were selected using univariate approach experiments,and then central composite design(CCD).The optimal extraction conditions were as follows:methanol concentration of 65%,microwave power of 200 W,and extraction time of 260 s.A high performance liquid chromatography–diode array detector(HPLC–DAD) method was established to quantitatively analyze these phytochemicals in different lotus plumule samples and in different part of *** separation was carried out on an Agilent Zorbax Extend-C_(18) column(4.6 mm×150 mm,3.5 μm).Gradient elution was applied with the mobile phase constituted with 0.1% triethylamine in water(A)and acetonitrile(B):40%-70% B at 0-8 min,70%-100% B at 8–9 min,100% B for 2 min,and then equilibrated with 40% B for 2 min.