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Identification and expression analysis of chlorogenic acid biosynthesis key gene PpHCT in peach

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Horticultural Plant Journal 2023年第4期9卷 670-680页

作者： Ziwen Su Meng Sun Zhixiang Cai shaolei guo Jiyao Li Bintao Zhao Zhijun Shen Ruijuan Ma Juan Yan Mingliang Yu College of Horticulture Nanjing Agricultural UniversityNanjingJiangsu 210095China Institute of Pomology Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjingJiangsu 210014China

Shikimic acid/quinic acid hydroxy cinnamyl transferase(HCT)is one of the key enzymes in the phenylpropanoid pathway.However,the role of the HCT gene in chlorogenic acid(CGA)biosynthesis in peach fruit remains unclear.For this,we identified the accumulation pattern of CGA in four peach cultivars,cloned and characterized 11 PpHCT gene members,and further analyzed the expression patterns of these PpHCT genes during fruit development.The contents of CGAs in the four peach cultivars all exhibited a trend of increasing and then decreasing during the fruit growth and development.Moreover,the contents of CGAs in the peel and flesh were tissue-specific.Gene structure analysis indicated that the PpHCT genes were highly conserved,containing two exons and one intron.The protein structure analysis demonstrated that the PpHCT proteins contained two conserved motifs(HXXXD,DFGWG)and a transferase domain(PF02458),which belonged to the BAHD acyltransferase family.The cis-acting element analysis suggested that the promoters of PpHCT genes contained many light-related,hormone-related,stress-related,tissue-specific,and circadian-related elements,and they could participate in a variety of biological processes.Phylogenetic analysis showed that the HCT proteins of peach were closely related to the HCT proteins of plum and had a close evolutionary relationship.The qRT-PCR analysis indicated that the expression levels of PpHCT1 and PpHCT2 showed an opposite trend to the accumulation of CGA,whereas the expression levels of PpHCT4,PpHCT5,PpHCT7,PpHCT8,and PpHCT11 demonstrated the same trend as CGA accumulation.It was worth noting that only PpHCT4 and PpHCT5 were highly expressed in the two high-CGA cultivars but showed low levels of expression in the two low-CGA cultivars.Therefore,it was hypothesized that these two genes might be key genes to the synthesis of CGA in peach fruit.Those findings provide a theoretical basis for further study on the biological functions of the HCT gene and help to reveal the molecular mechanism of CGA.

关键词： Prunus persica(L.) Chlorogenic acid PpHCT Bioinformatics analysis Gene expression

Differentiation of embryonic versus adult rat neural stem cells into dopaminergic neurons in vitro

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Neural Regeneration Research 2008年第8期3卷 832-836页

作者： Chunlong Ke Baili Chen shaolei guo Chao Yang Department of Neurosurgery First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 Guangdong Province China Department of Gastroenterology First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080 Guangdong Province China

BACKGROUND：It has been reported that the conversion of neural stem cells into dopaminergic neurons in vitro can be increased through specific cytokine combinations. Such neural stem cell-derived dopaminergic neurons could be used for the treatment of Parkinson’s disease. However, little is known about the differences in dopaminergic differentiation between neural stem cells derived from adult and embryonic rats. OBJECTIVE： To study the ability of rat adult and embryonic-derived neural stem cells to differentiate into dopaminergic neurons in vitro. DESIGN： Randomized grouping design. SETTING： Department of Neurosurgery in the First Affiliated Hospital of Sun Yat-sen University. MATERIALS： This experiment was performed at the Surgical Laboratory in the First Affiliated Hospital of Sun Yat-sen University （Guangzhou, Guangdong, China） from June to December 2007. Eight, adult, male, Sprague Dawley rats and eight, pregnant, Sprague Dawley rats （embryonic day 14 or 15） were provided by the Experimental Animal Center of Sun Yat-sen University. METHODS： Neural stem cells derived from adult and embryonic rats were respectively cultivated in serum-free culture medium containing epidermal growth factor and basic fibroblast growth factor. After passaging, neural stem cells were differentiated in medium containing interleukin-1α, interleukin-11, human leukemia inhibition factor, and glial cell line-derived neurotrophic factor. Six days later, cells were analyzed by immunocytochemistry and flow cytometry. MAIN OUTCOME MEASURES： Alterations in cellular morphology after differentiation of neural stem cells derived from adult and embryonic rats; and percentage of tyrosine hydroxylase-positive neurons in the differentiated cells. RESULTS： Neural stem cells derived from adult and embryonic rats were cultivated in differentiation medium. Six days later, differentiated cells were immunoreactive for tyrosine hydroxylase. The percentage of tyrosine hydroxylase positive neurons was （5.6 ± 2.8）% and （17.8 ± 4.2）% for adult and embryonic c

关键词： neural stem cells differentiation dopaminergic neurons

基于广泛靶向代谢组的桃果实衰老软化过程差异代谢物筛选与鉴定

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江苏农业科学 2024年第6期52卷 198-205页

作者：郭绍雷许建兰张斌斌张妤艳沈志军马瑞娟俞明亮江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室江苏南京210014

筛选与鉴定参与调控桃果实采后贮藏过程衰老软化相关代谢物质,以深入了解桃果实采后衰老软化相关生理生化机制。常温贮藏条件下,萘乙酸处理可加速霞晖8号七成熟桃果实衰老软化,采用超高液相色谱串联质谱技术,检测桃果实贮藏0、3、6 d代... 详细信息

筛选与鉴定参与调控桃果实采后贮藏过程衰老软化相关代谢物质,以深入了解桃果实采后衰老软化相关生理生化机制。常温贮藏条件下,萘乙酸处理可加速霞晖8号七成熟桃果实衰老软化,采用超高液相色谱串联质谱技术,检测桃果实贮藏0、3、6 d代谢物质的变化,选取差异倍数值(≥2或≤0.5),采用正交偏最小二乘判别分析模型所获得的变量重要性投影值(≥1)的代谢物为差异代谢物。结果表明,在霞晖8号桃果实5组样品中共检测到816种代谢物,可被分为11类,其中检测到酚酸类物质160种,占比最高。通过比较在各分组中代谢物定量信息的差异倍数变化前10的代谢物,结合KEGG信号通路的差异代谢物聚类分析,筛选并鉴定到可能参与桃果实软化的各种代谢物29种,其中脂质与酚酸类物质鉴定到最多,各有7种。

关键词：桃果实衰老软化代谢组差异代谢物

Intelligent power distribution live‐line operation robot systems based on stereo camera

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High Voltage 2023年第6期8卷 1306-1318页

作者： Yutao Chen Yahao Wang Xuming Tang Kai Wu shaolei Wu Rui guo Yu Feng Erbao Dong Department of Precision Machinery and Precision Instrumentation University of Science and Technology of ChinaHefeiChina State Grid Anhui Electric Power Company HefeiChina State Grid Anhui Electric Power Company Electric Power Research Institute HefeiChina State Grid Intelligent Technology Co. LtdJinanChina

Maintenance tasks in distribution networks are often accompanied by hazards associated with high altitudes and high voltages.By utilising robots instead of human operators to perform these tasks,potential risks can be avoided,while productivity can be increased.This research proposes an intelligent power distribution live‐line operation robot(PDLOR)system based on a stereo camera to replace human to complete work.The PDLOR system consists of several key components,including dual manipulators,wireless tools,a visual perception system,an insulated bucket truck,and a ground control ter-minal.Once the task is confirmed,the real‐time vision system identification and posi-tioning enable the adjustment of the insulated bucket to position the robot correctly for its intended work.The stereo camera plays a crucial role in accurately recognising and estimating the object's orientation.Additionally,a simplified reconstruction is performed within a virtual simulation environment,which aids in collision detection during path planning.After obtaining the optimal path,it is then communicated to the real manip-ulator for execution.To validate the feasibility of the PDLOR system,field experiments were conducted in actual distribution network scenarios.The results demonstrate that the PDLOR effectively completes single‐phase power‐line connection tasks within a remarkable 10‐min timeframe.

关键词： robot distribution stereo

A Large Acute Gastroenteritis Outbreak Associated with Both Campylobacter coli and Human Sapovirus—Beijing Municipality,China,2021

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China CDC weekly 2023年第52期5卷 1167-1173页

作者： Lin Zou Ying Li Guilan Zhou Zhenzhou Huang Changyan Ju Chunyan Zhao Xiang Gao Bojun Zhen Ping Zhang Xiaochen guo Jing Zhang Yang Zhang Bo Liu shaolei Zhou Aixia Yan Ying Kang Yanchun Wang Hongmei Ma Xiaohui Li Maojun Zhang Tongzhou District Center for Disease Control and Prevention BeijingChina Shunyi District Center for Disease Control and Prevention BeijingChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases National Institute for Communicable Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Nanshan District Center for Disease Control and Prevention Shenzhen CityGuangdong ProvinceChina

What is already known about this topic?Campylobacter is a significant foodborne pathogen that leads to global outbreaks of acute gastroenteritis(AGE)usually affecting less than 30 individuals.Human sapovirus(HuSaV)is an enteric virus responsible for sporadic cases and outbreaks of AGE worldwide.In a study conducted in Beijing,HuSaV detection ranked second after norovirus.What is added by this report?We present a discussion of the first large-scale outbreak of AGE caused by both Campylobacter coli(C.coli)and HuSaV.The outbreak involved a total of 996 patients and exhibited two distinct peaks over a period of 17 days.Through case-control studies,we identified exposure to raw water from a secondary water supply system as a significant risk factor.Among 83 patients,49 samples tested positive for C.coli,39 samples tested positive for HuSaV,and 27 samples tested positive for both pathogens using real-time polymerase chain reaction detection.Furthermore,whole-genome sequencing of 17 C.coli isolates obtained from 17 patients revealed that all isolates belonged to a highly clonal strain of C.coli.What are the implications for public health practice?Outbreaks of AGE resulting from multiple pathogen infections warrant increased attention.This report emphasizes the significance of ensuring the safety of drinking water,particularly in secondary supply systems.

关键词： Campylobacter Human enteritis

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负载量对桃叶片光合特性及果实品质的影响

果树学报 2022年第6期39卷 1004-1016页

作者：王晓俊张斌斌马瑞娟郭绍雷郭磊俞明亮江苏省农业科学院果树研究所·江苏省高效园艺作物遗传改良重点实验室南京210014 南京农业大学园艺学院南京210095

【目的】探究不同负载量对桃叶片光合特性、果实品质及树体生长发育的影响,以期为桃树的合理负载提供理论依据。【方法】以9年生霞晖8号桃为试材,在5月初(定果后2 d)、6月上旬(硬核期)、7月下旬(采前10 d)、8月初(采后2 d)4个时期测定... 详细信息

【目的】探究不同负载量对桃叶片光合特性、果实品质及树体生长发育的影响,以期为桃树的合理负载提供理论依据。【方法】以9年生霞晖8号桃为试材,在5月初(定果后2 d)、6月上旬(硬核期)、7月下旬(采前10 d)、8月初(采后2 d)4个时期测定不同负载量下成熟叶片光合特性、光合产物含量;果实成熟时,测定果实相关品质指标;冬季落叶后调查花芽、叶芽数量及不同结果枝数量。【结果】负载量下降,叶片叶绿素含量、净光合速率、水分利用效率、表观光能利用效率、表观CO_(2)利用效率和可溶性糖及糖醇含量总体均呈下降趋势,但果实大小、单果质量、果实可溶性固形物含量、糖酸比、可溶性糖及糖醇含量均显著升高。此外,随着负载量降低,树体花芽数量增加,叶芽数量减少;枝类组成中,长果枝的比例增加,短果枝则相反。【结论】综合比较认为,田间栽培中,霞晖8号桃成年树留果50%左右(约8500个·666.7 m^(-2)),可获得较好的果实品质,能保证较高的产量,且有助于树体的营养积累。

关键词：桃负载量光合特性果实品质生长发育

累及眶颅的鼻窦骨化纤维瘤手术治疗及磷酸钙骨水泥的修复应用

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中华耳鼻咽喉头颈外科杂志 2023年第12期58卷 1248-1253页

作者：钟华郑念真陈枫虹史剑波文卫平李健郭少雷中山大学附属第一医院耳鼻咽喉科、中山大学耳鼻咽喉科学研究所、广州市耳鼻咽喉科学重点实验室广州510080 中山大学附属第一医院神经外科广州510080

报告中山大学附属第一医院耳鼻咽喉科2019年12月至2020年6月收治的3例累及眶颅的鼻窦骨化纤维瘤,均行手术治疗,其中1例为经眉弓内镜下切除,2例为经冠状切口+内镜辅助切除,术中完全切除肿瘤组织,并选用磷酸钙骨水泥行颅底和眶周重建。3... 详细信息

报告中山大学附属第一医院耳鼻咽喉科2019年12月至2020年6月收治的3例累及眶颅的鼻窦骨化纤维瘤,均行手术治疗,其中1例为经眉弓内镜下切除,2例为经冠状切口+内镜辅助切除,术中完全切除肿瘤组织,并选用磷酸钙骨水泥行颅底和眶周重建。3例患者均达到完全切除,并一期行磷酸钙骨水泥颅底眶周重建,术后影像学复查提示肿瘤完全切除,颅底眶周修复完好,无视力下降、复视、脑脊液鼻漏等手术并发症。

关键词：耳鼻咽喉科脑脊液鼻漏磷酸钙骨水泥冠状切口眶周眶颅眉弓内镜辅助

可调节重力抗虹吸装置在脑积水分流术中的应用

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中国神经精神疾病杂志 2022年第6期48卷 342-346页

作者：章昌明宣若恒杨超郭少雷刘金龙中山大学附属第一医院神经外科广州510080

目的探讨可调节重力抗虹吸装置在脑积水分流术中使用的安全性及有效性。方法纳入初次诊断脑积水(17例)或侧脑室腹腔分流术(ventriculo-peritoneal shunt,VPS)后出现过度引流(6例)的患者,植入可调节重力抗虹吸装置,定期随访记录患者分流... 详细信息

目的探讨可调节重力抗虹吸装置在脑积水分流术中使用的安全性及有效性。方法纳入初次诊断脑积水(17例)或侧脑室腹腔分流术(ventriculo-peritoneal shunt,VPS)后出现过度引流(6例)的患者,植入可调节重力抗虹吸装置,定期随访记录患者分流阀压、影像学脑室变化及临床症状变化等。结果17例初次诊断脑积水患者植入可调压分流阀(商品名:proGAV)+可调节重力抗虹吸装置(商品名:proSA)后,其proGAV阀门和proSA阀门平均压分别为(92.4±3.0)mmH_(2)O和(23.0±0.8)cmH_(2)O,头部影像示Evan指数由术前0.34±0.01降为0.28±0.01;额角直径/同一水平颅骨内板间径比(frontal horns/internal diameter,FH/ID)由术前0.44±0.01降为0.36±0.01,差异均具有统计学意义(P<0.05),提示术后脑室系统明显缩小;17例患者术后临床症状双下肢乏力及尿失禁均明显改善(3例昏迷患者除外)。另6例首次未植入抗重力阀的脑积水患者VPS分流术后出现头晕恶心呕吐等过度引流症状,遂植入proSA,其Codman阀门压由术前(170.0±9.5)mmH_(2)O降低为(120.0±8.4)mmH_(2)O,差异有统计学意义(P<0.05);头部影像示额枕/颅径比(fronto-occipital horn ratio,FOHR)由0.23±0.02升至0.37±0.01,差异有统计学意义(P<0.05),提示脑室系统由裂隙样形态恢复至基本正常;6例患者过度引流临床症状明显缓解。结论在临床初诊脑积水或VPS术后过度引流等复杂脑积水患者中植入可调节重力抗虹吸装置调控脑脊液引流是安全且有效的。

关键词：脑积水侧脑室腹腔分流术(VPS) 过度引流可调节重力抗虹吸装置(proSA)

Different Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures

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Horticultural Plant Journal 2016年第2期2卷 75-81页

作者： YANG Yong MA Ruijuan ZHANG Binbin SONG Zhizhong ZHANG Chunhua guo shaolei YU Mingliang Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement Institute of Horticulture Jiangsu Academy of Agricultural Sciences College of Horticulture Nanjing Agricultural University

The aim of our study was to assess differences in the expression of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage conditions. Different peach cultivars of ‘Xiacui' and ‘Yumyeong', which are stonyhard, ‘Yinhualu', which is softmelting, ‘Hujing Milu', which is hard-melting, and ‘Baby Gold 6', which is non-melting at 80% ripening, were collected as test materials. The results showed that only slight ethylene production was detected after harvesting of ‘Yumyeong' and ‘Xiacui' under either a room temperature(25 °C) or low temperature(4 °C). The fruit firmness of stonyhard cultivars was retained at a high level under room temperature over time, whereas a low temperature induced ‘Yumyeong' fruit to soften. Quantitative real-time PCR results indicated that the PpACS1 gene was highly expressed in soft-melting, hard-melting and non-melting cultivars; however, expression was extremely low in stonyhard peaches. PpACS2 or PpACS3, however,was not detected in all five cultivars. Interestingly, cold treatment significantly decreased firmness along with endo-PG expression obviously upregulated in ‘Yumyeong', but not in ‘Xiacui' peaches. In conclusion, this study revealed that fruit softening of peaches with different flesh textures was closely related to ethylene biosynthesis during the storage period, which was controlled via regulating relevant gene expression levels under different storage temperatures.

关键词： Prunus persica ethylene biosynthetic pathway soften gene expression