Identification and expression analysis of chlorogenic acid biosynthesis key gene PpHCT in peach

作     者：Ziwen Su Meng Sun Zhixiang Cai Shaolei Guo Jiyao Li Bintao Zhao Zhijun Shen Ruijuan Ma Juan Yan Mingliang Yu Ziwen Su;Meng Sun;Zhixiang Cai;Shaolei Guo;Jiyao Li;Bintao Zhao;Zhijun Shen;Ruijuan Ma;Juan Yan;Mingliang Yu

作者机构：College of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China Institute of PomologyJiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic ImprovementNanjingJiangsu 210014China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2023年第9卷第4期

页      面：670-680页

核心收录：

学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学] 

基　　金：supported by the funds of the Natural Science Foundation of Jiangsu Province(Grant No.BK20200278) the China Agriculture Research System(Grant No.CARS-30) the Species Conservation Project of Ministry of Agriculture and Rural Affair(Grant No.19210137) the National Crop Germplasm Resources Infrastructure in China(Grant No.NHGRC2021-NH16) 

主　　题：Prunus persica(L.) Chlorogenic acid PpHCT Bioinformatics analysis Gene expression 

摘      要：Shikimic acid/quinic acid hydroxy cinnamyl transferase(HCT)is one of the key enzymes in the phenylpropanoid ***,the role of the HCT gene in chlorogenic acid(CGA)biosynthesis in peach fruit remains *** this,we identified the accumulation pattern of CGA in four peach cultivars,cloned and characterized 11 PpHCT gene members,and further analyzed the expression patterns of these PpHCT genes during fruit *** contents of CGAs in the four peach cultivars all exhibited a trend of increasing and then decreasing during the fruit growth and ***,the contents of CGAs in the peel and flesh were *** structure analysis indicated that the PpHCT genes were highly conserved,containing two exons and one *** protein structure analysis demonstrated that the PpHCT proteins contained two conserved motifs(HXXXD,DFGWG)and a transferase domain(PF02458),which belonged to the BAHD acyltransferase *** cis-acting element analysis suggested that the promoters of PpHCT genes contained many light-related,hormone-related,stress-related,tissue-specific,and circadian-related elements,and they could participate in a variety of biological *** analysis showed that the HCT proteins of peach were closely related to the HCT proteins of plum and had a close evolutionary *** qRT-PCR analysis indicated that the expression levels of PpHCT1 and PpHCT2 showed an opposite trend to the accumulation of CGA,whereas the expression levels of PpHCT4,PpHCT5,PpHCT7,PpHCT8,and PpHCT11 demonstrated the same trend as CGA *** was worth noting that only PpHCT4 and PpHCT5 were highly expressed in the two high-CGA cultivars but showed low levels of expression in the two low-CGA ***,it was hypothesized that these two genes might be key genes to the synthesis of CGA in peach *** findings provide a theoretical basis for further study on the biological functions of the HCT gene and help to