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Isolation,identification,and virulence gene analysis of pathogenic Aeromonas dhakensis in Macrobrachium rosenbergii and histopathological observation

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Journal of Oceanology and Limnology 2024年第2期42卷 664-675页

作者： Xin PENG Haihui TU Xinyi YAO Xuan LAN Zhenxiao ZHONG Jinping LUO Qiongying TANG shaokui YI Zhenglong XIA Guoliang YANG Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development College of Life SciencesHuzhou UniversityHuzhou 313000China Jiangsu Shufeng Prawn Breeding Co. Ltd.Gaoyou 225654China

To identify the cause of mass mortality of adult Macrobrachium rosenbergii in a farm in Gaoyou City,Jiangsu Province,China,a dominant strain named DKQ-1 was isolated from the hepatopancreas of dying M.rosenbergii and identified as Aeromonas dhakensis by purification culture,biochemical characterization,and 16S rRNA and gyrB gene sequence analysis.The results of the challenge test revealed that the strain was highly pathogenic and the 50%lethal dose(LD_(50))in 72 h to M.rosenbergii was 1.54×10^(5)CFU/mL.The amplification results of virulence genes show that strain DKQ-1 carried 9 virulence genes,including ascV,aexT,aer,act,lip,ompAI,gcaT,acg,and exu,supporting the strong virulence of strain DKQ-1 to M.rosenbergii.Histopathological observation of the hepatopancreas,gills,and intestines indicated that DKQ-1 injection into M.rosenbergii could cause serious tissue damage,which further supported the strong virulence of this strain.In addition,a drug susceptibility test revealed that strain DKQ-1 was sensitive to 16 kinds of antibiotics,resistant to 9 kinds of antibiotics,and had intermediate resistance to spectinomycin and kanamycin.This study is the first report of A.dhakensis isolated from M.rosenbergii and provided a reference for the pathogen identification of bacterial diseases in M.rosenbergii,and for the prevention and treatment caused by A.dhakensis.

关键词： Aeromonas dhakensis histopathology virulence gene gyrB drug susceptibility test Macrobrachium rosenbergii

Genetic parameter evaluation for growth and cold resistance traits of the giant freshwater prawn Macrobrachium rosenbergii

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Journal of Oceanology and Limnology 2024年第2期42卷 609-619页

作者： Haihui TU Qianqian XING Zhenxiao ZHONG Qiongying TANG shaokui YI Zhenglong XIA Miaoying CAI Jingfen LI Quanxin GAO Guoliang YANG Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development College of Life SciencesHuzhou UniversityHuzhou 313000China Fengjiashan Reservoir Administration Bureau Baoji 721300China Jiangsu Shufeng Prawn Breeding Co.Ltd. Gaoyou 225654China

The giant freshwater prawn Macrobrachium rosenbergii distributed from tropical to subtropical regions,is a warm-water species,and its survival temperature is 14-35°C,which greatly limits its culture cycle and culture area in China.Therefore,it is urgent to cultivate a new high quality,high yield variety with improved cold-resistance,but the genetic parameters for cold-resistance traits are unknown in M.rosenbergii.In this study,the cold-resistance of adult M.rosenbergii populations was tested using the indoor artificial cooling method.Individuals were selected from 139 families of Shufeng G3 generation and cultured for 200 days.A linear mixed model was constructed by ASReml-R to evaluate the genetic parameters of the cold-resistance trait(cooling degree hours,CDH)and growth traits(body weight,BW,and body length,BL)based on the restricted maximum likelihood(REML)method.The results show that the heritability of CDH was low(0.12±0.05),while the growth traits(BW and BL)had low to moderate heritability,with 0.20±0.06 for BW and 0.06±0.04 for BL.The phenotypic and genetic correlation between BW and BL was significantly positive,but significantly negative phenotypic and genetic correlations were detected between CDH and BW and between CDH and BL.Furthermore,the analysis of the differences between cold-resistance and phenotypic traits showed that the female reproductive status,exoskeleton hardness and claw number of adult prawns had a great influence on the cold-resistance of M.rosenbergii(P<0.05),indicating that adults with claws and hard exoskeletons are preferred as parents in subsequent breeding selection.The present results can be attributed to the selection and breeding of a new cold-resistant variety of M.rosenbergii.

关键词： cold-resistance growth traits heritability correlation Macrobrachium rosenbergii

Natural variations in grain length 10(GL10)regulate rice grain size

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Journal of Genetics and Genomics 2022年第5期49卷 405-413页

作者： Penglin Zhan Shuaipeng Ma Zhili Xiao Fangping Li Xin Wei Shaojun Lin Xiaoling wang Zhe Ji Yu Fu Jiahao Pan Mi Zhou Yue Liu Zengyuan Chang Lu Li Suhong Bu Zupei Liu Haitao Zhu Guifu Liu Guiquan Zhang shaokui wang Guangdong Laboratory for Lingnan Modern Agriculture GuangzhouGuangdong 510642China State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Provincial Key Laboratory of Plant Molecular BreedingSouth China Agricultural UniversityGuangzhouGuangdong 510642China Department of Plant Sciences University of OxfordOxford OX13RBUK

Grain size is an important determinant of grain weight and yield in rice.Although several genes related to grain size have been identified,natural variations in these genes that affect grain size are poorly characterized.Here,we describe the grain length QTL GL10,encoding MADS56,which positively regulates grain length and grain weight.A natural allelic variation of NIL-gl10,containing an~1.0-kb deletion in the first exon that abolishes its transcription,results in shorter grain length,lower grain weight and delayed flowering in gl10 plants.The knockout of GL10 in the HJX74 background leads to grain phenotypes similar to that of NIL-gl10,while overexpression of GL10 results in increased grain length and weight and earlier heading date.GL10 regulates grain length by promoting greater longitudinal cell growth in the grain glume.Additionally,GL10 participates in the regulation of gibberellic acid(GA)signaling pathway genes in young panicle tissues.Analysis of GL10 haplotypes shows obvious divergence between the japonica and indica lineages.Our findings reveal an allelic variation of GL10 that may explain differences in grain length among modern cultivars and could be used to breed rice varieties with optimized grain shape.

关键词： Rice GL10 OsMADS56 Grain length SSSL GA

Methylesterification of cell-wall pectin controls the diurnal flower-opening times in rice

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Molecular Plant 2022年第6期15卷 956-972页

作者： Mumei wang Xiaopei Zhu Guoqing Peng Minglong Liu Shuqing Zhang Minghao Chen Shitang Liao Xiaoying Wei Peng Xu Xiyu Tan Fangping Li Zhichuan Li Li Deng Ziliang Luo Liya Zhu Shuai Zhao Dagang Jiang Jing Li Zhenlan Liu Xianrong Xie shaokui wang Aimin Wu Chuxiong Zhuang Hai Zhou Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesCollege of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China Agronomy Department University of FloridaGainesvilleFL 32610USA State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Life Science and TechnologyGuangxi UniversityNanning 530004China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources College of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642China

Flowers are the core reproductive organ of plants, and flowering is essential for cross-pollination. Diurnal flower-opening time is thus a key trait influencing reproductive isolation, hybrid breeding, and thermostability in plants. However, the molecular mechanisms controlling this trait remain unknown. Here, we report that rice Diurnal Flower Opening Time 1 (DFOT1) modulates pectin methylesterase (PME) activity to regulate pectin methylesterification levels of the lodicule cell walls, which affect lodicule swelling to control diurnal flower-opening time. DFOT1 is specifically expressed in the lodicules, and its expression gradually increases with the approach to flowering but decreases with flowering. Importantly, a knockout of DFOT1 showed earlier diurnal flower opening. We demonstrate that DFOT1 interacts directly with multiple PMEs to promote their activity. Knockout of PME40 also resulted in early diurnal flower opening, whereas overexpression of PME42 delayed diurnal flower opening. Lower PME activity was observed to be associated with higher levels of pectin methylesterification and the softening of cell walls in lodicules, which contribute to the absorption of water by lodicules and cause them to swell, thus promoting early diurnal flower opening. Higher PME activity had the opposite effect. Collectively, our work uncovers a molecular mechanism underlying the regulation of diurnal flower-opening time in rice, which would help reduce the costs of hybrid breeding and improve the heat tolerance of flowering plants by avoiding higher temperatures at anthesis.

关键词： rice hybrid breeding flowering diurnal flower-opening times cell wall pectin methylesterase

破局、重构与协同:生成式人工智能视域下的“破五唯”审思

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高校教育管理 2024年第2期18卷 53-64页

作者：肖珺广少奎李滋阳曲阜师范大学教育学院山东济宁273165 江苏科技大学期刊社江苏镇江212100 浙江传媒学院文化创意与管理学院浙江杭州310018

以ChatGPT为代表的生成式人工智能以其革命性的技术创新对当下以“破五唯”为旨归的高校学术评价改革产生了诸多影响。凭借自然语言的人机交互模式、机器深度学习能力,生成式人工智能改进了传统低效的科研方式,推动高校学术评价拓宽了... 详细信息

以ChatGPT为代表的生成式人工智能以其革命性的技术创新对当下以“破五唯”为旨归的高校学术评价改革产生了诸多影响。凭借自然语言的人机交互模式、机器深度学习能力,生成式人工智能改进了传统低效的科研方式,推动高校学术评价拓宽了评价维度。与此同时,高质量的人工智能内容产出消解了论文作为学术评价标准的权威性,进而形成对“五唯”体系的破局之势。在此基础上,依托新型数智技术,生成式人工智能在数据采集、分析和反馈环节表现出强大的信息抓取、数据整合和关联分析能力,能够切实解决评价新方法在数据收集和处理上的难题,推动学术评价方法的重构与落地。生成式人工智能技术在“破五唯”上潜力初现,但是在制度、技术和应用环节上存在的风险与不足亦不容忽视。相关管理部门、高校、评价机构和科技公司亟须加强政策引导和机构监管、推进基础建设和学科发展、改进思维模式和工作方法,在新技术与旧制度磨合变革的过程中与智能机器协同共进、提质增效。

关键词： “破五唯” 生成式人工智能学术评价改革 ChatGPT 高校

2007–2020年湖南会同杉木人工林生物量数据集

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中国科学数据（中英文网络版） 2024年第2期9卷 218-226页

作者：关欣黄苛颜绍馗中国科学院沈阳应用生态研究所会同森林生态实验站湖南会同418307 亚热带森林生态系统结构与服务功能湖南省重点实验室湖南会同418307

杉木人工林是我国面积最大,蓄积量最多的人工林。通过对湖南会同森林生态系统国家野外科学观测研究站(简称会同站)杉木人工林综合观测场生物量数据的长期监测,本数据集整理了2007–2020年湖南会同杉木人工林生物量数据,具体包括树干、... 详细信息

杉木人工林是我国面积最大,蓄积量最多的人工林。通过对湖南会同森林生态系统国家野外科学观测研究站(简称会同站)杉木人工林综合观测场生物量数据的长期监测,本数据集整理了2007–2020年湖南会同杉木人工林生物量数据,具体包括树干、树枝、树叶、果(花)、树皮和气生根的干重。本数据集通过野外调查数据,结合生物量模型计算生物量。野外调查和实验流程严格遵照CERN的规范执行,数据的质量控制和评估由台站监测人员、数据管理员和分中心质控人员共同完成。本数据集的建立和共享主要为全球变化背景下杉木人工林生物量研究提供数据支持,对深入了解杉木人工林生态系统的结构和功能特征,制定合理的杉木人工林经营措施具有重要意义。

关键词：杉木人工林生物量长期监测

GL9 from Oryza glumaepatula controls grain size and chalkiness in rice

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The Crop Journal 2023年第1期11卷 198-207页

作者： Shaojun Lin Zupei Liu Kui Zhang Weifeng Yang Penglin Zhan Quanya Tan Yajun Gou Shuaipeng Ma Xin Luan Chubing Huang Zhili Xiao Yuanyuan Liu Bihuang Zhu Ruiqing Liang Wenqi Zhou Haitao Zhu Suhong Bu Guifu Liu Guiquan Zhang shaokui wang Guangdong Provincial Key Laboratory of Plant Molecular Breeding State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Laboratory for Lingnan Modern Agriculture Guangzhou 510630GuangdongChina

Grain size is a key factor influencing grain yield and appearance quality in rice.We identified twelve quantitative trait loci(QTL)for grain length(GL),nine for grain width(GW),and nine for 1000-kernel weight(TKW)using GLU-SSSLs,which are single-segment substitution lines with Oryza glumaepatula as donor parent and Huajingxian 74(HJX74)as recipient parent.Among the QTL,qGL1-2,qGL1-4,qGL9-2,qGW2-2,qGW9-1 and qTKW9-2 contributed to high grain yield.GL9 was identified as a candidate gene for qGL9-2 by map-based cloning and sequencing,and is a novel allele of GS9.The kernel of NIL-gl9was slenderer and longer than that of HJX74,and the TKW and grain yield per plant of NIL-gl9 were higher than those of HJX74.The proportion of grain chalkiness of NIL-gl9 was much lower than that of HJX74.Thus,gl9 increased grain yield and appearance quality simultaneously.Three pyramid lines,NIL-gs3/gl9,NIL-GW7/gl9 and NIL-gw8/gl9,were developed and the kernel of each was longer than that of the corresponding recipient parent lines.The gl9 allele may be beneficial for breeding rice varieties with high grain yield and good appearance quality.

关键词： Oryza glumaepatula GL9 Grain size Grain chalkiness Single-segment substitution line

血流限制训练法联合抗阻训练对运动者肌肉相关指标影响的Meta分析

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中国组织工程研究 2025年第8期29卷 1705-1713页

作者：张孜贤徐有粮吴绍奎王相英山东师范大学体育学院山东省济南市250358 华东师范大学体育与健康学院上海市200241 贵州大学体育学院贵州省贵阳市550025

目的:通过收集血流限制训练法联合抗阻训练的相关文献,依照系统评价和Meta分析的范式,分析血流限制训练法联合抗阻训练与抗阻训练对运动者肌肉相关指标和专项能力的不同影响,旨在为运动者在训练实践中运用血流限制训练提供数据支持。方... 详细信息

目的:通过收集血流限制训练法联合抗阻训练的相关文献,依照系统评价和Meta分析的范式,分析血流限制训练法联合抗阻训练与抗阻训练对运动者肌肉相关指标和专项能力的不同影响,旨在为运动者在训练实践中运用血流限制训练提供数据支持。方法:检索中外数据库(中国知网、万方、PubMed、Web of Science和SPORTDiscus),应用血流限制训练法联合抗阻训练对对象为运动者中大学生运动者肢体围度、肌肉质量、肌肉力量和专项运动能力影响的随机对照试验,检索起止时间为2000-01-01/2023-10-12。至少2名研究者采用Cochrane协作网偏倚风险评估工具和标准对纳入文献进行质量评价。使用RevMan 5.4软件进行异质性检验、数据合并、亚组分析、绘制森林图和敏感性分析,绘制漏斗图并进行发表偏倚评价和敏感性分析。评价指标为肢体围度、肌肉厚度、肌肉力量和专项能力等,对不同专项运动能力进行亚组分析。结果:①共纳入18项随机对照试验,共403例受试者,根据Cochrane协作网偏倚风险评估工具,纳入文献中文献质量为A级的有16篇,B级有2篇。②将血流限制训练法联合抗阻训练与抗阻训练之间的效果进行对比,在肢体围度方面,两组间无显著性差异(SMD=0.03,95%CI:-0.16-0.21,P=0.78);在肌肉厚度方面(SMD=0.14,95%CI:0.01-0.27,P=0.03)及肌肉力量方面(SMD=0.37,95%CI:0.14-0.60,P=0.001)两组间有显著性差异。③对专项能力指标进行亚组分析结果显示,距离指标的分析结果存在高异质性(I2=73%),时间指标的分析结果存在高异质性(I2=55%),分析可能原因是各研究的测试方法及评估指标意义的不同导致;功率指标的分析结果显示无异质性(I2=0%);血流限制训练法联合抗阻训练对距离指标具有显著影响(P<0.01)。④合并效应结果显示,血流限制训练法联合抗阻训练比较抗阻训练对于专项能力的影响(P=0.41),提示不同训练方法对于专项能力不存在显著性影响。结论:①两种训练方法均能够促进大学生运动者的肌肉厚度、肌肉力量和专项能力,血流限制训练法联合抗阻训练相较于抗阻训练在促进运动员肌肉厚度、肌肉力量和部分专项能力方面具有显著性效果。因此,可以在专项训练之中科学合理地融合血流限制训练法,以差异性的生理刺激综合作用于肌肉,以取得更好的训练效果。②然而,由于纳入的研究数量较少,以及其他可能存在的局限性,今后需要纳入更多的高质量、多项目类型和性别的随机对照试验来证实。

关键词：血流限制训练法抗阻训练大学生运动者肢体围度肌肉厚度肌肉力量专项能力 Meta分析

积极老龄化背景下主动健康与体育促进:内涵、定位与个性方案

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山东体育学院学报 2024年第2期40卷 18-27页

作者：徐有粮张孜贤吴绍奎王相英熊文华东师范大学体育与健康学院上海200241 山东师范大学体育学院山东济南250358 贵州大学体育学院贵州贵阳550025

在全球老龄化背景下,推动老年人主动健康是时代之需也是国家之需。运用文献资料、逻辑推理等研究方法,探究主动健康与体育促进之间的逻辑、定位与策略,对实现健康中国和积极应对人口老龄化国家战略目标,助推中国式现代化建设具有重要的... 详细信息

在全球老龄化背景下,推动老年人主动健康是时代之需也是国家之需。运用文献资料、逻辑推理等研究方法,探究主动健康与体育促进之间的逻辑、定位与策略,对实现健康中国和积极应对人口老龄化国家战略目标,助推中国式现代化建设具有重要的意义。研究认为:体育促进老年群体主动健康在价值、导向和目的三方面形成内涵耦合,体现出主动健康与体育促进在内在取向、发展规律和共同愿景之间的紧密联系;体育在促进老年群体主动健康中的定位亟待厘清,应从体育促进主动健康的有限性和限制性、个体亲身参与和长期坚持的必要性两方面精确定位。研究提出:(1)体育促进老年群体主动健康的个性化方案设计应依照个体特征的多维差异、运动方案的功能差异以及个体的适应性差异,遵循安全性、有效性和可持续性原则。(2)老年群体个性化运动方案的制定需要遵循运动筛查、测评、制定目标、个性化指导、计划调试等基本流程,重视运动方案设计以及数据分析和长期追踪,为老年群体制定适当、有效的个性化运动方案。

关键词：积极老龄化主动健康体育促进个性化运动方案