Development of a platform for breeding by design of CMS lines based on anSSSL library in rice(Oryza sativaL.)
作者单位：State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesSouth China Agricultural University
会议名称：《广东省遗传学会第九届代表大会暨学术研讨会》
会议日期：2014年
基 金：supported by grants from the National Natural Science Foundation of China(U1031002) the major program of transgenic new variety breeding of China(2014ZX08009-037B)
关 键 词：Single segment substitution line Cytoplasmic male sterility Breeding by design Rice
摘 要：Cytoplasmic male sterility(CMS) is an important foundation for heterosis utilization andexploited widely to hybrid seed production in ***,the traditional experiencedriven and labor-cost breeding style left little room for sustainable improvement ofhybrid *** of molecular biology and genomics provides a chance toenhance hybrid rice breeding efficiency through breeding by *** this study,aline H121 B with rj3 and rf4 genes from XieqingzaoB(XB) was developed in theHuajingxian74(HJX74) genetic *** H121 B as maintainer line,threeisonuclear alloplasmic CMS lines,W-H121 A,D-H121 A and Y-H121 A,with the wildabortive(WA),dwarf wild abortive(DA),and Yegong abortive(YA) types of CMScytoplasm,respectively,were *** H121 B maintainer line was thenimproved by a way of breeding by *** favorable alleles,gs3 for long grain,Wxlfor low amylose content,and OsMADSSO for early heading date,in the chromosomalsubstituted segments of single segment substitution lines(SSSLs) were selected fromthe SSSL library in the HJX74 genetic background,and were pyramided together inH121 B through marker-assisted selection(MAS).The new maintainer line,H131 B,showing the phenotypes of longer in grain length,lower in amylose content,and earlierin heading date than those in H121 B,achieved the breeding goal ***131 B as maintainer line,three new isonuclear alloplasmic CMS lines,W-H131 A,DH131A and Y-H131 A,were *** results indicate that breeding by designin rice can be conducted under the platform of the HJX74 SSSL *** the platform for breeding by design of CMS lines will be helpful for sustainableimprovement of CMS lines in hybrid rice development.