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蜡样芽孢杆菌YSQ08与角质蛋白酶联合降解羽毛的研究

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饲料广角 2016年第19期 46-49页

作者：朱辉 mazu shanti 朱剑锋胡文锋华南农业大学食品学院生物源生物技术(深圳)有限公司

对蜡样芽孢杆菌YSQ08、角质蛋白酶、酶菌联合降解羽毛3种方式进行比较,结果表明酶菌联合降解羽毛的效果更突出.深入研究蜡样芽孢杆菌YSQ08、角质蛋白酶添加顺序、菌培养时间以及酶添加时间对羽毛降解率的影响,结果显示采用菌+酶的方式... 详细信息

对蜡样芽孢杆菌YSQ08、角质蛋白酶、酶菌联合降解羽毛3种方式进行比较,结果表明酶菌联合降解羽毛的效果更突出.深入研究蜡样芽孢杆菌YSQ08、角质蛋白酶添加顺序、菌培养时间以及酶添加时间对羽毛降解率的影响,结果显示采用菌+酶的方式能降解更多的羽毛,同时数据表明菌液在培养14h时产酶达到高峰,加入菌液后的24h再添加角质蛋白酶能保持其较高的活力.以pH值、羽毛添加量、酶添加量、菌液添加量、培养天数为试验因素,设计L16(45)正交试验,结果表明在pH9.0、羽毛添加量3.0%、酶添加量2000U/g、菌液添加量1.5%、培养时间为96h条件下,发酵结果最优,此时氨基酸含量为223.26mg/L,降解率为81.76%.菌+酶降解羽毛的方式为工业化处理羽毛废弃物提供新的思路.

关键词：羽毛联合降解添加时间 YSQ08 蜡样芽孢杆菌蜡样芽胞杆菌角蛋白酶降解率角质

Study on Degradation of Feather by Bacillus Cereus YSQ08 and Keratinase

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Study on Degradation of Feather by Bacillus Cereus YSQ08 and...

作者： mazu Sabirath shanti 华南农业大学

学位级别：硕士

对蜡样芽孢杆菌YSQ08、角质蛋白酶、酶菌联合降解羽毛三种方式进行比较,结果酶菌联合降解羽毛的效果更突出。深入研究蜡样芽孢杆菌YSQ08、角质蛋白酶添加顺序、菌培养时间以及酶添加时间对羽毛降解率的影响,结果采用菌+酶的方式能降解... 详细信息

对蜡样芽孢杆菌YSQ08、角质蛋白酶、酶菌联合降解羽毛三种方式进行比较,结果酶菌联合降解羽毛的效果更突出。深入研究蜡样芽孢杆菌YSQ08、角质蛋白酶添加顺序、菌培养时间以及酶添加时间对羽毛降解率的影响,结果采用菌+酶的方式能降解更多的羽毛;同时数据表明菌液在培养14h时产酶达到高峰,加入菌液后的24h再添加角质蛋白酶能保持其较高的活力。以pH值、羽毛添加量、酶添加量、菌液添加量、培养天数为试验因素,设计L(4)正交试验,结果表明在pH 9.0、羽毛添加量3.0%、酶添加量2000 U/g、菌液添加量1.5%、培养时间为96 h条件下,发酵结果最优,此时氨基酸含量为223.26 mg/L,降解率为81.76%。pH 9.0、羽毛添加量3.0%、酶添加量3000 U/g、菌液添加量3.0%、培养时间为4 d为各自的因素的均值最值,以此为条件进行验证试验,结果表明,此时氨基酸含量为273.57 mg/L,降解率为85.41%。以最优发酵条件制备发酵液,取部分发酵液进行5000 r/min离心10 min,得到发酵上清液、发酵沉淀物;对其真空冷冻干燥,得到发酵液干物质、上清液干物质、沉淀物干物质,其蛋白质含量分别为87.35%、41.43%、92.36%。蜡样芽孢杆菌YSQ08联合角蛋白酶降解鸡羽毛后得到的发酵上清液干物质饲养黑水虻效果优于猪粪、发酵沉淀干物质以及发酵液干物质。其饲养黑水虻到一半预蛹时的平均增重为0.2399g,料重比为2.6341、转化率为38.19%、存活率为90.79%。

关键词：蜡样芽孢杆菌YSQ08 角质蛋白酶羽毛发酵黑水虻

Kainate receptor channel opening and gating mechanism

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四川生理科学杂志 2024年第5期46卷 1137-1137页

作者： shanti Pal Gangwar 不详

Kainate receptors,a subclass of ionotropic glutamate receptors,are tetrameric ligand-gated ion channels that mediate excitatory *** receptors modulate neuronal circuits and synaptic plasticity during the development and function of the central nervous system and are implicated in various neurological and psychiatric diseases,including epilepsy,depression,schizophrenia,anxiety and *** structures of kainate receptor domains and subunit assemblies are available12-18,the mechanism of kainate receptor gating remains poorly *** we present cryo-electron microscopy structures of the kainate receptor GluK2 in the presence of the agonist glutamate and the positive allosteric modulators lectin concanavalin A and BPAM344.

关键词： epilepsy diseases mechanism

Updates in therapeutic drug monitoring in inflammatory bowel disease

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World Journal of Gastroenterology 2022年第21期28卷 2282-2290页

作者： Nilesh Lodhia shanti Rao Department of Gastroenterology and Hepatology Atrium HealthCharlotteNC 28203United States

Biologics and immunomodulators(IMM)are generally considered the most effective therapies for the treatment of ulcerative colitis and Crohn’s ***,despite the efficacy of these therapies,many patients either have a primary lack of response or a secondary loss of response to these *** drug monitoring(TDM)is a systematic approach to managing such *** this review,we summarize the latest data on TDM,including reactive and proactive TDM,in patients with inflammatory bowel disease on biologics and/or IMM.

关键词： Inflammatory bowel disease Therapeutic drug monitoring Crohn’s disease Ulcerative colitis

Convection–Diffusion Model for the Prediction of Anthropogenically-Initiated Wildfire Ignition

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International Journal of Disaster Risk Science 2014年第4期5卷 274-295页

作者： Ravi Sadasivuni shanti Bhushan William H.Cooke Department of Geosciences Mississippi State UniversityStarkvilleMS 39759USA Center for Advance Vehicular Systems Mississippi State UniversityStarkvilleMS 39759USA

A spatial interaction model to predict anthropogenically-initiated accidental and incendiary wildfire ignition probability is developed using fluid flow analogies for human movement patterns. Urban areas with large populations are identified as the sites of global influencing factors, and are modeled as the gravity term. The transportation corridors are identified as local influencing factors, and are modeled using fluid flow analogy as diffusion and convection terms. The model is implemented in ArcGIS, and applied for the prediction of wildfire hazard distribution in southeastern Mississippi. The model shows87 % correlation with historic data in the winter season,whereas the previously developed gravity model shows only 75 % correlation. The normalized error for convection–diffusion model predictions is about 5 % in the winter season, whereas the gravity model shows an error of 7 %.The proposed model is robust as it couples a multi-criteria behavioral pattern within a single dynamic equation to enhance predictive capability. At the same time, the proposed model is more costly than the gravity model as it requires evaluation of distance from intermodal transportation corridors, transportation corridor density, and traffic volume maps. Nonetheless, the model is developed in a modular fashion, such that either global or local terms can be neglected if required.

关键词： Anthropogenic-fire Convection–diffusion model Fire ignition potential Mississippi Wildfire hazard

Photometric study of an eclipsing binary in Praesepe

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Research in Astronomy and Astrophysics 2013年第4期13卷 465-470页

作者： Devarapalli shanti Priya Kandulapati Sriram Pasagada Vivekananda Rao Department of Astronomy Osmania University Korea Astronomy and Space Science Institute

We present CCD photometric observations of an eclipsing binary in the direction of the open cluster Praesepe using the 2 m telescope at IUCAA Girawali Observatory, India. Though the system was classified as an eclipsing binary by Pepper et al., detailed investigations have been lacking. The photometric solutions using the Wilson-Devinney code suggest that it is a W-type W UMa system and, interestingly, the system parameters are similar to another contact binary system SW Lac.

关键词： binaries: eclipsing - binaries: close -open clusters: Praesepe

Photometric study of an eclipsing binary in the field of M37

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Research in Astronomy and Astrophysics 2014年第9期14卷 1166-1172页

作者： Devarapalli shanti Priya Kandulapati Sriram Pasagada Vivekananda Rao Department of Astronomy Osmania University Hyderabad 500 007 India

CCD photometric observations with B and V passbands were performed on the contact binary V3 in the field of open cluster M37. The solutions were obtained for data from both B and V passbands along with R passband given by Hartman et al. using the Wilson-Devinney code. The positive O＇Connell effect was observed in all the three passbands and its associated cool spot parameters were derived. The results indicate that the spot parameters have not shown any significant variability during the last four years. The spot radius was found to be 40° and located close to the equator of the secondary component. The absolute parameters of the system were derived using the empirical relations given by Gazeas et al.

关键词： binaries: eclipsing -- binaries: close

Photometric study of eclipsing binaries in the Large Magellanic Cloud--I.W UMa type binaries in the Large Magellanic Cloud

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Research in Astronomy and Astrophysics 2011年第2期11卷 175-180页

作者： Devarapalli shanti Priya Kandulapati Sriram Pasagada Vivekananda Rao Department of Astronomy Osmania University Korea Astronomy and Space Science Institute

Eclipsing binaries are among the most important sources of information on stellar parameters like radii,masses,luminosities,*** present the analysis of six W UMa systems discovered in the Large Magellanic Cloud using ... 详细信息

关键词： binaries: eclipsing -- binaries: close

酶菌联用降解羽毛工艺优化及降解产物对黑水虻幼虫生长性能的影响

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饲料工业 2017年第22期38卷 41-46页

作者： mazu Sabirath shanti 林俊宏朱剑锋朱辉李楚君庞旭董璐胡文锋华南农业大学广东广州510642 汕头职业技术学院广东汕头515078 生物源生物技术(深圳)股份有限公司广东深圳518118 广州无两生物科技有限公司广东广州510642

试验采用角蛋白酶和蜡样芽孢杆菌联用处理鸡羽毛,以羽毛降解率为指标,研究了不同pH值、酶添加量、细菌接种量、羽毛添加量和发酵时间对羽毛降解率的影响,优化了降解羽毛的工艺条件。之后分别使用三种降解产物和猪粪作为饲料,研究了羽毛... 详细信息

试验采用角蛋白酶和蜡样芽孢杆菌联用处理鸡羽毛,以羽毛降解率为指标,研究了不同pH值、酶添加量、细菌接种量、羽毛添加量和发酵时间对羽毛降解率的影响,优化了降解羽毛的工艺条件。之后分别使用三种降解产物和猪粪作为饲料,研究了羽毛降解产物对黑水虻幼虫生长性能的影响,包括平均增重、料重比和存活率等。结果表明,适宜的酶菌联用降解羽毛工艺条件为:pH值9.0、酶添加量2 000 U/g、蜡样芽孢杆菌接种量3.0%(v/v)、羽毛添加量2.5%,发酵5 d。使用发酵上清液干物质作为饲料养殖黑水虻幼虫,能有效提高黑水虻幼虫平均增重、优化料重比、提高存活率。

关键词：角蛋白酶蜡样芽孢杆菌羽毛黑水虻幼虫