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Journal of Oceanology and Limnology 2024年第2期42卷 580-593页

作者： Shun ZHANG Xiaojing ZHU Lingzhu HU Kai LIAO shanliang xu Danli WANG Chunyang GUO School of Marine Science Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China

The Tbx family is first known through the study of their functions in the body and limbs,and its members Tbx4 and Tbx5 genes are important factors in determining the characteristics of the *** argenteus is one of the important economical marine fishes widely distributed in offshore ***,it is necessary to study the role of Tbx family genes in the deletion of pelvic fin in *** this study,we cloned Tbx4 and Tbx5 cDNA sequence of ***(GenBank:MH709128 and MH712458).The Western blot and real time PCR were used to detect the expressions of Tbx4 and Tbx5 in different developmental stages and tissues of *** addition,whole-mount in-situ hybridization was used to study the localization of Tbx4 and Tbx5 genes in different developmental stages of *** show that the translation of Tbx4 mRNA was inhibited during the critical period of pelvic fin *** different tissues,Tbx4 protein levels were the lowest in the abdominal epithelium,and even lower than that in the pectoral fin,suggesting that the protein expression was also inhibited in the abdominal epithelium of adult ***,the results indicated that upstream genes regulation led to the key stage-specific and low expression of Tbx4 during pelvic fin development and in the abdominal epithelium.

关键词： Stromateidae pelvic fin protein translation T-box aquaculture broodstock

Optimal Culture Capacity of White Shrimp (Litopenaeus vannamei) and Razor Clam (Sinonovacula constricta) in a New Series-Connection Culture Model

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Journal of Ocean University of China 2022年第6期21卷 1641-1648页

作者： HU Jiabao ZHAO Chunpu BAO Gege LUO Yunhui WANG Danli xu Jilin xu shanliang School of Marine Sciences Ningbo UniversityNingbo 315211China

To construct an effective circular mix-culture model and investigate its culture capacity,we established one small and one large systems,with white shrimp(Litopenaeus vannamei)and razor clam(Sinonovacula constricta)cultured in separate *** culture water from the *** was pumped to the ***,and the culture water from the *** overflowed back into the *** via *** trial I,we tested four culture densities(groups 1–4),and monitored water quality,growth indices,and digestive and immune enzyme *** the results,the nitrogen and phosphorus levels generally increased then declined after 56 days,and were lower in groups 1 and *** specific growth rate of group 2 was the *** 56 days,activities of four digestive enzymes were increased in group 2,and lysozyme activity was significantly decreased in *** in groups 1–4;Alkaline phosphatase activity of *** was increased in group 3,but decreased in *** in groups 2–4;Acid phosphatase activity was significantly higher in groups 1–3(P<0.05),while SOD and CAT activities were significantly elevated in group 2(P<0.05).Thus,we applied group 2 density for trial *** trial II,nitrogen and phosphorus concentrations declined significantly during the latter stages,and growth indices were higher than the control(P<0.05).The yields of *** and *** were significantly higher than the control(P<0.05).The results revealed a good circular mix-culture system with the optimal culture capacity for ***(40 ind m^(−2))and ***(300 ind m^(−2)),which provided a reference for the future culture of them.

关键词： Litopenaeus vannamei Sinonovacula constricta culture capacity series connection culture model water quality

Expression and localization of vitellogenin genes(VTG)and receptor(VGR)in the gonad development of silver pomfret Pampus argenteus

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Journal of Oceanology and Limnology 2023年第4期41卷 1575-1592页

作者： Zitao XIONG Jiazhe YANG Shun ZHANG Yajun WANG shanliang xu Chunyang GUO Danli WANG School of Marine Sciences Ningbo UniversityNingbo 315211China Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China

Vitellogenesis is the main event of oocyte growth in oviparous animals,which is mainly manifested by the accumulation of vitellogenin(VTG).The accumulation of vitellogenin depends mainly on the absorption of exogenous vitellogenin,which enters oocyte through endocytosis mediated by its receptor(VGR).We investigated the expression and localization of VTG and VGR during gonad development of Pampus *** qPCR results show that vtgs were not expressed in male fish,but in the ovary and liver of female fish;the expression levels went up at first and then *** expression levels of vgr in the testis were low and only 1%-3%of that in *** results show that during the ovarian development of ***,VTG in liver,serum,and ovary all showed a trend from increasing to ***,VTG in liver peaked in StageⅣ,and in serum and ovary peaked in Stage V,reflecting changes in the characteristics of VTG in the liver(synthesis),blood(transport),and ovaries(accumulation).During gonad development,VGR in the ovaries first increased and then decreased,reaching a peak in Stage V,in contrast to vgr mRNA *** VGR content in the testis was extremely low and stable,consistent with vgr *** results show that the location and intensity of VTG and VGR positive signals were synchronized with the changes of their protein content,which revealed that VTG was mainly synthesized in the liver cytoplasm,secreted into the blood,and transported to ovary in StageⅢ.VGR is highly expressed in oocytes in StageⅡ.In StageⅢ,a large amount of VTG reaches the ovary,when VGR begins to translate and is subsequently transported to the plasma membrane of the ***,the positive signal of VGR was stronger near the plasma membrane of oocytes in StagesⅠandⅡ.By using qPCR,ELISA,and immunohistochemistry,the synthesis,transport,and accumulation of vitellogenin were elucidated and the mechanism of its endocytosis on egg membrane mediated by VTG during the de

关键词： Pampus argenteus vitellogenin(VTG) vitellogenin receptor(VGR) vitellogenesis

Life cycle of Craspedacusta sowerbyi xinyangensis

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Current Zoology 2009年第3期55卷 227-234页

作者： shanliang xu Danli WANG Key laboratory of Applied Marine Biotechnology Ministry of Education Ningbo University Ningbo 315211 Zhejiang China Key Laboratory of Marine Biotechnology of Zhejiang Ningbo 315211 Zhejiang China

To explore the life cycle of Craspedacusta,the authors collected male and female specimens of the Craspedacusta sowerbyi xinyangensis in a small fire-fighting pond in Ningbo,Zhejiang Province in July,2005 and *** development of *** xinyangensis was studied from zygote to medusa by means of light microscopy and digital *** zygotes of *** xinyangensis are globular and smooth(90-105 μm diameter)and have an equal,total cleavage to the two-cell stage 15 min after *** embryos enter the four-cell stage after another 15 min and become multicellular embryos after 3h *** this stage the embryos have a diameter similar to fertilized eggs but have uneven surfaces that are distinct from the smooth surfaces of the uncleaved *** gastrulae are formed 7 h after *** are spherical planulae with short surface cilia that begin to swim in slow clockwise *** 12 h,they lose their cilia,cease swimming and become elongated planulae with one end larger than the ***-like planulae,similar in thickness at both ends,are formed after an additional 7 *** 4 days,the planulae develop into tiny polyps having two germ layers and a gastrovascular *** polyp mouth is 50-62 μm in diameter,lacking tentacles but having nematocysts around the *** become mature polyps after 10 days(15 days after fertilization).Medusa buds(45-88 μm diameter)are formed by polyp budding,which soon become free-living medusae with 8 tentacles(380-620 μm diameters).Sometimes,the movement of frustules,which are formed by the polyps and similar to planulae in morphology can also be observed.

关键词：桃花水母受精卵细胞动物学裂解

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基尼指数结合K均值聚类的协同训练算法

重庆师范大学学报（自然科学版） 2022年第4期39卷 134-140页

作者：徐善亮吕佳重庆师范大学计算机与信息科学学院重庆师范大学重庆市数字农业服务工程技术研究中心重庆401331

【目的】针对在标准协同训练中不具有充分冗余的视图分割,致使分类器错误累计过多,以及一对基分类器标记样本类别不一致的问题,提出了基尼指数结合K均值聚类的协同训练算法。【方法】该算法首先计算已标记样本中各特征的基尼指数,将该... 详细信息

【目的】针对在标准协同训练中不具有充分冗余的视图分割,致使分类器错误累计过多,以及一对基分类器标记样本类别不一致的问题,提出了基尼指数结合K均值聚类的协同训练算法。【方法】该算法首先计算已标记样本中各特征的基尼指数,将该指数升序排列后均等划分到两个视图,然后在基分类器所标记的样本中,运用K均值聚类确定标记不一致样本的类别后加入标记样本。【结果】通过9个UCI数据集在3组实验上的结果表明,所提算法相较于对比算法提升了分类效果。【结论】运用基尼指数均等划分关键特征于两个视图,有利于改善视图分割不充分冗余的缺陷;K均值聚类法对分类不一致样本进行重新标记,降低了协同训练算法中的误标记率。

关键词：半监督学习协同训练基尼指数 K均值聚类

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褐菖鲉仔、稚鱼消化系统发育的组织学观察

热带海洋学报 2019年第2期38卷 58-66页

作者：杨佳喆齐闯徐善良宁波大学海洋学院浙江宁波315211 应用海洋生物技术教育部重点实验室宁波大学浙江宁波315211

为探究卵胎生鱼类褐菖鲉(Sebastiscusmarmoratus)的仔、稚鱼消化系统组织学特点,采用常规组织切片技术,并用HE染色和组织化学染色,比较分析了产出后0～50d的褐菖鲉仔、稚鱼消化系统发育的组织学变化。结果显示:褐菖鲉初产仔鱼已分化出... 详细信息

为探究卵胎生鱼类褐菖鲉(Sebastiscusmarmoratus)的仔、稚鱼消化系统组织学特点,采用常规组织切片技术,并用HE染色和组织化学染色,比较分析了产出后0～50d的褐菖鲉仔、稚鱼消化系统发育的组织学变化。结果显示:褐菖鲉初产仔鱼已分化出口咽腔,并具有初始的食道、胃、肠、肝脏和胰脏; 2日仔鱼肛门与外界相通,食道扩大,开始摄食,进入混合性营养期; 3～4日仔鱼幽门盲囊出现,食道内壁出现黏膜皱褶5～6个,胃黏膜皱褶5个,肝细胞团区域扩大; 5～6日仔鱼卵黄囊和油球耗尽,进入外源性营养期,食道黏膜上皮出现杯状细胞,肠道弯曲,可区分前中肠和后肠两部分;10～14日仔鱼食道环肌层明显,黏膜皱褶增加到7～12个,胃壁四层结构基本形成,肠道黏膜皱褶加深,纹状缘清晰可见,肝细胞分裂迅速,数量显著增加,体积增大,出现肝血窦和胰岛; 28～30日稚鱼出现胃腺和胃小凹,已具功能性胃,标志着稚鱼期的开始,此时胰岛细胞数量和酶原颗粒增多,消化能力显著提高;47～50日稚鱼已基本具有成鱼胃的特征,肠道纹状缘发达,肝细胞呈多边形,细胞内含大量脂肪颗粒,消化系统从结构和功能上已趋于完善。由此可见,褐菖鲉仔鱼和稚鱼消化系统的发育具有卵胎生鱼类发育较早的特点,与其消化功能的完善密切相关。

关键词：褐菖鲉仔稚鱼消化系统组织学

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脱二氧化碳装置对循环水养殖系统pH的影响

渔业现代化 2017年第1期44卷 10-14页

作者：巩建华曹潇徐善良宁波大学海洋学院应用海洋生物技术教育部重点实验室浙江宁波315211

为了调节循环水系统中养殖水体的pH,根据气体交换原理,设计一种脱二氧化碳(CO_2)装置。采用该装置去除养殖水体中的CO_2,并对由于CO_2含量累积造成的pH下降进行调节,使养殖鱼类处在一个适宜的pH环境中。试验时水温控制在(25±0.5)℃,每... 详细信息

为了调节循环水系统中养殖水体的pH,根据气体交换原理,设计一种脱二氧化碳(CO_2)装置。采用该装置去除养殖水体中的CO_2,并对由于CO_2含量累积造成的pH下降进行调节,使养殖鱼类处在一个适宜的pH环境中。试验时水温控制在(25±0.5)℃,每1 h取水样测1次pH,每4 h测1次碱度。水样取自养鱼桶内的水,检测前先对水样用40μm孔径针头过滤器进行过滤处理,实验周期24 h。结果显示,循环水系统加装脱二氧化碳装置能有效去除CO_2,使水体稳定在一个适宜的pH范围(7.39~7.42);CO_2质量浓度呈降低趋势,24 h后由开始的13.16 mg/L降低到7~8 mg/L,降低近50%,而不加装脱二氧化碳装置的循环水系统CO_2质量浓度持续上升,24 h后增加到37 mg/L左右,pH持续降低,最终降低到6.72~6.81。研究表明,脱二氧化碳装置能够有效去除水体中的CO_2,使水体pH维持在一个适宜鱼类生长的范围。

关键词：脱二氧化碳装置 CO2 pH 循环水养殖系统

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缢蛏动态能量收支生长模型的建立

水产学报 2021年第4期45卷 578-587页

作者：骆云慧吴波徐善良徐继林王丹丽宁波大学海洋学院宁波大学海洋生物工程浙江省重点实验室

为建立缢蛏动态能量收支(dynamic energy budget,DEB)的数值模型,实验根据DEB理论,采用相应方法测定计算了建模必须的形状系数(δm)、阿伦纽斯温度(TA)、单位时间单位体积维持生命所需的能量[p M]、形成单位体积结构物质所需的能量[EG]... 详细信息

为建立缢蛏动态能量收支(dynamic energy budget,DEB)的数值模型,实验根据DEB理论,采用相应方法测定计算了建模必须的形状系数(δm)、阿伦纽斯温度(TA)、单位时间单位体积维持生命所需的能量[p M]、形成单位体积结构物质所需的能量[EG]和单位体积最大储存能量[EM]等基本参数。以水温和叶绿素浓度作为强制函数,针对围塘水质因子与春秋两季放养的缢蛏生长数据,利用STELLA 10.0软件构建了缢蛏的DEB模型。结果显示,缢蛏δm值为0.345 8,TA平均值为(5 448±960) K,[p M]=17.68 J/(cm~3·d),[EG]=4 050 J/cm~3,[EM]=1 840 J/cm~3;构建的DEB模型可以较好地模拟缢蛏软体部干重的生长,并且反映了不同时间的能量分配情况。春季放苗的缢蛏,在8月10日—9月20日,水温(29.8～32.2°C)为其主要生长限制因子;在9月30日—11月10日,食物为主要限制因子。秋季放苗的缢蛏,则在7月1日—9月20日表现出强烈的温度限制(30.5～32.2°C),而食物限制贯穿于整个模拟期间,在3月10日—5月20日限制最大。故秋季放苗的缢蛏更应在春季加大藻的培育,在夏季高温前达到上市规格。

关键词：缢蛏生长动态能量收支模型养殖环境

银鲳应对高温胁迫的生理响应及其相关基因表达研究

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应用海洋学学报 2022年第1期41卷 1-7页

作者：史琛榆赵淳朴胡艺潇沙桃平王亚军王丹丽徐善良宁波大学海洋学院浙江宁波315211 宁波大学应用海洋生物技术教育部重点实验室浙江宁波315211

为探究银鲳(Pampus argenteus)应对高温胁迫的生理响应及其相关基因表达研究,本研究设置4个温度组:对照组26℃和实验组28、30、32℃。并对银鲳的肝、肾和鳃的组织结构、肝组织中超氧化物歧化酶、过氧化氢酶的相对活性变化和丙二醛含量... 详细信息

为探究银鲳(Pampus argenteus)应对高温胁迫的生理响应及其相关基因表达研究,本研究设置4个温度组:对照组26℃和实验组28、30、32℃。并对银鲳的肝、肾和鳃的组织结构、肝组织中超氧化物歧化酶、过氧化氢酶的相对活性变化和丙二醛含量变化以及hsp70基因的相对表达量的变化进行了研究。结果显示:(1)随温度的上升,3种组织的组织结构损伤程度逐步加深,且肝、鳃组织表现更为明显且复杂;(2)高温显著影响了银鲳抗氧化酶活性,短期热胁迫时,银鲳对28℃能逐步适应,而在30℃和32℃高温胁迫下,则会对银鲳机体产生不可逆的损伤;(3)随应激时间的延长,不同温度下hsp70基因的表达量均呈先上升后下降的变化趋势,均在0.5天时显著升高并达到峰值,之后迅速回落。研究表明:短期热胁迫时,银鲳对28℃能逐步适应,而在30℃下,胁迫将对其产生严重损伤;32℃高温胁迫下,银鲳表现出强烈的应激响应,胁迫对其造成致命损伤。

关键词：海洋生物学银鲳高温胁迫组织损伤酶活性 hsp70基因表达

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信阳桃花水母对几种生态因子胁迫的耐受反应

应用生态学报 2006年第6期17卷 1103-1106页

作者：王丹丽徐善良蒋宏雷杨华宁波大学生命科学与生物工程学院宁波315211 宁波市海洋与渔业研究院宁波315010

在不同温度、盐度、pH等因子胁迫条件下,研究观察了信阳桃花水母（Craspedacusta sowerbyi xinyangensis）的生活状态、形态变化及存活等耐受反应,并测定了信阳桃花水母在不同温度下的耗氧率和最低耐氧能力.结果表明,信阳桃花水母的最... 详细信息

在不同温度、盐度、pH等因子胁迫条件下,研究观察了信阳桃花水母（Craspedacusta sowerbyi xinyangensis）的生活状态、形态变化及存活等耐受反应,并测定了信阳桃花水母在不同温度下的耗氧率和最低耐氧能力.结果表明,信阳桃花水母的最适生活温度范围为15 ℃～25 ℃,pH为6.0～8.2.信阳桃花水母对盐度耐受力差,盐度为2时,仅能存活96 h;但具有较强的耐低氧能力,20 ℃时窒息点为0.26 mg·L^-1,平均耗氧量和平均耗氧率随温度的升高而升高.

关键词：桃花水母生态因子胁迫耐受性耗氧率