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Cell Research 1995年第2期5卷 209-220页

作者： SHEN EH XIA SUN (shanghai Brain Research Institute, Chinese Academy of Sciences, shanghai 200031, China.) shanghai Brain Research Institute Chinese Academy of Sciences Shanghai 200031 China.

Hemorrhage or hypotension induces extensive Foslike immunoreactivity in the magnocellular neurosecretory cells in the supraoptic nucleus of the hypothalamus in rat, especially in the vasopressin neurons. The present study was to explore the neurotransmitter mediating this effect. Microinfusion of the alpha-adrenergic blocker into the supraoptic nucleus reduced the hypotension-induced Fos, whereas beta-antagonist did not affect it significantly. Alpha1- and alpha2-antagonist, prazosin and yohimbine,both reduced the Fos-positive cell counts. However, the effective dosage of yohimbine was much larger. Alpha1-agonist, methoxamine, induced abundant Fos-like immnnoreactivity in the vasopressin cells in this nucleus,while beta-and alpha2-agonist did not elicit such *** of the noradrenergic re-uptake inhibitor,desipramine, to this nucleus to locally accumulate the spontaneously released noradrenaline from the nerve terminals also induced Fos expression, mostly in the vasopressin cells.

关键词： c-fos neurosecretory cells noradrenaline supraoptic nucleus vasopressin

Partial Synthesis of Macrolide Alkaloid Euonine

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Chinese Chemical Letters 1998年第10期9卷 927-928页

作者： Yuan Chao LI Shan Hao JIANG Xiao Yi WANG (shanghai Institute of Materia Medica, Chinese Academy of Sciences, shanghai, 200031 Department of Environmental Chemistry and Engineering, Beijing Polytechnic University,Beijing, 100022) Chinese Acad Sci Shanghai Inst Mat Med Shanghai 200031 Peoples R China Beijing Polytech Univ Dept Environm Chem & Engn Beijing 100022 Peoples R China

The macrolide alkaloid euonine 1 with pharmacologic activity was prepared from the alkaloid wilforgine 2. Their structures were confirmed by IR, NMR, MS and optical rotation.

关键词： tripterygium macrolide alkaloid wilforgin euonine

STUDIES　ON　DOPAMINE　RECEPTORS　AND　TETRAHYDROPROTOBERBERINES．Ⅱ．QUANTUM　CHEMISTRY　OF　LIGAND－RECEPTOR　INTERACTION

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Chinese Chemical Letters 1996年第3期7卷 245-248页

作者： Yun TANG Kai Xian CHEN Hua Liang JIANG Guo Zhang JIN Ru Yun JI(shanghai Institute of Materia Medica, Chinese Academy of Sciences,294 Taiyuan Road, shanghai 200031) shanghai Institute of Materia Medica Chinese Academy of Sciences 294 Taiyuan Road Shanghai 200031

Based oil the models of dopanmine reccptors and tetrahydroprolobotherine *** carried cul semiempiricalquantum of cmical calculations on the active sites of rcceptors and ligands. The results showed that foe frontier melccular orbitals oflignod and receplor just matched cach *** suggerted that ligand and receptor might form charge-transfer interaction.

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Effects of cisplatin on telomerase activity and telomere length in BEL-7404 human hepatoma cells

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Cell Research 2002年第1期12卷 55-62页

作者： Ru GANG ZHANG, Ru PING ZHANG, XING WANG WANG, HONG XIE Department of Biotherapy, Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, shanghai 200031, China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China 1Department of Biotherapy Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences the Chinese Academy of Sciences Shanghai 200031 China

Telomerase activity was inhibited in a dose and time-dependent manner with the treatment of cisplatin for 24, 48, or 72 h in a concentration ranged from 0.8 to 50 1uM in BEL-7404 human hepatoma cells. There were no changes in expression pattern of three telomerase subunits, its catalytic reverse transcriptase subunit (hTERT), its RNA component (hTR) or the associated protein subunit (TP1), after cisplatin treated for 72 h with indicated concentrations. Mean telomere lengths were decreased by the cisplatin treatment. Cell growth inhibition and cell cycle accumulation in G2/M phase were found to be correlated with telomerase inhibition in the present study, but percentages of cell apoptosis did not change markedly during the process.

关键词： Telomerase telomere cisplatin hepatocellular carcinoma cell cycle cell growth.

Transgenic mice overexpressingγ-aminobutyric acid transporter subtypeⅠ develop obesity

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Cell Research 2000年第4期10卷 303-310页

作者： MA YING HUA JIA HUA HU XIAO GANG ZHOU RUO WANG ZENG ZHEN TONG MEI JIAN FEI LI HE GUO Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, Chinese Academy of Science, shanghai 200031, China shanghai Institute of harmacy.ankara.edu.tr

Transgenic mice ubiquitously overexpressing murine γ aminobutyric acid transporter subtype Ⅰ were created. Unexpectedly, these mice markedly exhibited heritable obesity, which features significantly increased body weight and fat deposition. Behavioral examination revealed that transgeinc mice have slightly reduced spontaneous locomotive capacity and altered feeding pattern. Tills preliminary finding indicates that the inappropriate level of γ-aminobutyric acid transporters may be directly or indirectly involved in the pathogenic mechanism underlying certain types of obesity.

关键词： γ-aminobutyric acid transporter subtype Ⅰ transgenic mouse obesit€

Identification of the development stage-specific factors in mouse fetal liver binding to the human β-globin gene promoter

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Cell Research 1994年第1期4卷 9-15页

作者： CHEN YADI YULONG HU RUOLAN QIAN(shanghai Institute of Cell Biology, Academia Sinica shanghai 200031, China.) shanghai Institute of Cell Biology Academia Sinica Shanghai China

In order to elucidate the molecular mechanisms of globin gene expression during embryonic development, the nuclear extracts from mouse hematopoietic tissue at different stages of development have been prepared. By using DNase I footprinting and gel mobility shift assays, the binding of protein factors in these extracts to the human βglobin promoter was analyzed. The differences in the binding patterns of protein factors during development were observed. An erythroid-specific and stage-specific nuclear protein in the nuclear extract from d 18 mouse fetal liver was identified, which can bind to the sequence (from -66bp to -90bp) of human β-globin promoter. We therefore speculate that the function of this cis-acting element may be similar to stage selector element (SSE) in chieken βA- promoter.

关键词： β-globin gene promoter trans-acting factor

Huperzine H, a New Lycopodium Alkaloid from Huperzia serrata

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Chinese Chemical Letters 1999年第6期10卷 463-466页

作者： Wen Yun GAO Yi M lug LI Bac De WANG Da Yuan ZHU(State Key Laboratory of New Drug Research. shanghai Institute of Materia Medica. ChineseAcademy of Sciences, shanghai 200031) Chinese Acad Sci Shanghai Inst Mat Med State Key Lab New Drug Res Shanghai 200031 Peoples R China

A new lycopodium alkaloid with a novel skeleton was isolated from Huperzia serrata. Its structure was determined on the basis of spectral evidences.

关键词： Huperzia serrata (Thunb.) Trev. huperzine H isolation structure

Overexpression of γ-aminobutyric acid transporter subtype I leads to susceptibility to Kainic acid-induced seizure in transgenic mice

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Cell Research 2001年第1期11卷 61-67页

作者： MA YINGHUA, JIA HUA HU, WEN JUAN ZHAO, JIAN FEI, YUN YU, XIAO GANG ZHOU, ZHEN TONG MEI, LI HE GUO (Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, shanghai 200031, China) (shanghai Inst 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China 2shanghai Institute of Physiology Chinese Academy of Sciences Shanghai 200031 China 1Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

γ-aminobutyric acid (GABA) is the principal inhibitory neurotransmitter, and the GABAergic synaptic transmission is normally terminated by the rapid uptake through GABA transporters. With transgenic mice ubiquitously overexpressing GABA transporter subtype I (GAT1), the present study explored the pathophysiological role of GAT1 in epileptogenesis. Though displaying no spontaneous seizure activity, these mice exhibit altered electroencephalographic patterns and increased susceptibility to seizure induced by kainic acid. In addition, the GABAA receptor and glutamate transporters are up-regulated in transgenic mice, which perhaps reflects a compensatory or corrective change to the elevated level of GAT1. These preliminary findings support the hypothesis that excitatory and inhibitory neurotransmission, and seizure susceptibility can be altered by neurotransmitter transporters.

关键词： γ-aminobutyric acid transporter seizure susceptibility kainic acid electroencephalography transgenic mice

Molecular characterization and functional expression of opioid receptor-libe1 receptor

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Cell Research 1997年第1期7卷 69-77页

作者： WU YA LAN LU PU KUN LING JIAN ZHAO ZHI JIE CHENG LAN MA GANG PEI(shanghai Institute of Cell Biology, Chinese Academy of Sciences, shanghai 200031, China)(shanghai Medical University, shanghai 200032, China) shanghai Medical University Shanghai 200032 China

The opioid receptor-libel receptor (ORL), an orphan receptor whose human and murine complementary DNAs,has been characterized recently. ORL transcripts are particularly abundant in the central nervous system. We demonstrated that ORL expressed in human neuroblastoma SK-N-SH and SH-SY5Y cell lines by radioligand binding assay, reverse transcription polymerase chain reaction (RT-PCR) and Northern analysis in the present study. Stimulation with ORL1 specific agonist, nociceptin/orphanin Fo, increased [34S]GTPrγS binding to SK-N-SH cell membranes (EC50 = 14 ±0.45 nM), and attenuated forskolin-stimulated accumulation of cellular cAMP (EC50= 0.80 ±0.45 nM, indicative that activation of ORL1 activates G proteins and inhibits adenylyl cyclase. Activation of ORL1 receptor was also accessed using CHO:hORL1 cell line by microphysiometer. Treatment of nociceptin/orphanin FQ increased extracellular acidification rate significantly.

关键词： Opioid receptor-libe1 receptor (ORL1) nociceptin/orphanin FQ (N/OFQ) SK-N-SH cells extracellular acidification functional expression

Computational neuroscience in China

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Science China(Life Sciences) 2010年第3期53卷 385-397页

作者： WU Si1 & LIANG PeiJi2 1Institute of Neuroscience,shanghai Institutes for Biological Sciences,State Key Laboratory of Neurobiology,Chinese Academy of Sciences,shanghai 200031,China 2Department of Biomedical Engineering,shanghai Jiaotong University,shanghai 200240,China 1. Institute of Neuroscience Shanghai Institutes for Biological Sciences State Key Laboratory of Neurobiology Chinese Academy of Sciences Shanghai 200031 China2. Department of Biomedical Engineering Shanghai Jiaotong University Shanghai 200240 China

The ultimate goal of Computational Neuroscience(CNS) is to use and develop mathematical models and approaches to elucidate brain *** is a young and highly multidisciplinary *** heavily interacts with experimental neuroscience and such other research areas as artificial intelligence,robotics,computer vision,information science and machine *** paper reviews the history of CNS in China,its current status and the prospects for its future *** of CNS research in China are also presented.

关键词： computational neuroscience experimental neuroscience artificial intelligence