Identification of the development stage-specific factors in mouse fetal liver binding to the human β-globin gene promoter
Identification of the development stage-specific factors in mouse fetal liver binding to the human β-globin gene promoter作者机构：Shanghai Institute of Cell Biology Academia Sinica Shanghai China
出 版 物：《Cell Research》 (细胞研究(英文版))
年 卷 期：1994年第4卷第1期
页 面：9-15页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学]
主 题：β-globin gene promoter trans-acting factor
摘 要：In order to elucidate the molecular mechanisms of globin gene expression during embryonic development, the nuclear extracts from mouse hematopoietic tissue at different stages of development have been prepared. By using DNase I footprinting and gel mobility shift assays, the binding of protein factors in these extracts to the human βglobin promoter was analyzed. The differences in the binding patterns of protein factors during development were observed. An erythroid-specific and stage-specific nuclear protein in the nuclear extract from d 18 mouse fetal liver was identified, which can bind to the sequence (from -66bp to -90bp) of human β-globin promoter. We therefore speculate that the function of this cis-acting element may be similar to stage selector element (SSE) in chieken βA- promoter.
同方期刊数据库