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Cell Research 1999年第1期9卷 51-59页

作者： ZHAO HUA YONG HUA XU (shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 yue-yang Road, shanghai 200031, China) 1shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China

Mad protein has been shown as an antagonist of cMyc protein in some cell lines. The effect of Mad protein to the malignant phenotype of human hepatoma BEL7404 cell line was investigated experimentally. An eukarryotic vector pCDNA Ⅲ containing full ORF fragmentof mad cDNA was transfected into targeted cells. Under G418 selection, stable Mad-overexpressed cells were *** on the effect of Mad over-expression in cell proliferation and cell cycle revealed that cell morphology of the Mad-overexpressed BEL-7404-M1 cells was significantly different from the parent and control vector transfected cells. DNA synthesis, cell proliferation and anchorage-independent growth in soft-agar of the madtransfected cel1s were partially inhibited in comparison to control *** Cytometry analysis indicated that mad over-expression might block more transfectant cells at G0/G1 phase, resulting in the retardation of cell proliferation. RT-PCR detected a marked inhibition of the expression of cdc25A, an important regulator gene of G0/G1to S phase in cell cycle. It was also found that Mad protein overexpression could greatly suppress p53-mediated apoptosis in BEL-7404-M1 cells in the absence of ***, Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL7404 *** and regulation of cell growth and apoptosis

关键词： Mad c-Myc cell growth apoptosis human hepatoma cells

Synthesis and Characteristics of an Aspartame Analogue,L-Asparaginyl L-3-Phenyllactic Acid Methyl Ester

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Acta Biochimica et Biophysica Sinica 2004年第6期36卷 385-389页

作者： Hu TAO1, Da-Fu CUI1, and You-Shang ZHANG1,2* 1Institute of Biochemistry and Cell Biology, shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, shanghai 200031, China Institute of Protein Research, Tongji University, shanghai 200031, China Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of SciencesShanghai 200031 China Institute of Protein Research Tongji UniversityShanghai 200031 China

An aspartame analogue, L-asparaginyl L-3-phenyllactic acid methyl ester was synthesized with aspartic acid replaced by asparagine and peptide bond replaced by ester bond. The aspartic acid of aspartame could be replaced by asparagine as reported in the literature. In this analogue, the hydrogen of amide group could still form a hydrogen bond with the oxygen of ester bond and the ester bond was isosteric with peptide bond. However, the product was not sweet, showing that the peptide bond could not be replaced by ester bond. The peptide C-N bond behaves as a double bond that is not free to rotate and the C, O, N and H atoms are in the same plane. The replacement of peptide bond by ester bond destroyed the unique conformation of peptide bond, resulting in the loss of sweet taste.

关键词： artificial sweetener aspartame derivative peptide bond

Functional analysis of schistosomes EF-hand domain-containing tegument proteins

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Chinese Science Bulletin 2007年第15期52卷 2100-2107页

作者： YU FuDong1,2,3,KANG Bin1,3,LI YuanYuan3,4 & LI YiXue1,3 1 Bioinformatics Center,Key Laboratory of Systems Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sci-ences,shanghai 200031,China 2 Graduate University of Chinese Academy of Sciences,shanghai 200031,China 3 shanghai Center for Bioinformation Technology,shanghai 200235,China 4 Key Laboratory of Systems Biology,shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,shanghai 200031,China Bioinformatics Center Key Laboratory of Systems Biology Shanghai Institutesfor Biological Sciences Chinese Academy of Sciences Shanghai 200031 China

Schistosomes cause schistosomiasis disease which severely threatens human health. Little is known about the functions of EF-hand domain containing schistosomes tegument proteins other than as an-tigens. More possible functions of these tegument proteins were investigated with in silico analyses including protein-protein functional interaction,site-specific variation and glycosylation modification. The analysis results suggested that schistosomes could actively modulate host immune responses for its own favor through functional interactions with host proteins with immunomodulatory function,and passively regulate host immune responses through sequence variation under positive selection and glycosylating the recognition sites of host immune attack. In addition,the analysis of the C-terminal domain of these tegument proteins indicated that they could assist schistosomes in escaping host immune attacks through inhibiting chemotaxis and non-complement fixing antibody (IgG4) responses. In summary,our results suggested that these tegument antigen proteins could assist schistosomes in escaping and modulating host immune responses for self-protection during the process of host-para-site interaction.

关键词：寄生虫血吸虫皮蛋白质健康

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Cure of experimental botulism and antibotulismic effect of toosendanin

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Acta Pharmacologica Sinica 2004年第6期25卷 137-146页

作者： Yu-liang SHI2, Zhong-feng WANG Key laboratory of Neurobiology, shanghai Institutes for Biological Sciences, Chinese Academy ofScience, shanghai200031, China Key laboratory of Neurobiology Shanghai Institutes for Biological Sciences Chinese Academy of Science Shanghai 200031 Chin

Botulinum neurotoxins (BoNTs), a group of bacterial proteins that comprise a light chain disulfide linked a heavy chain, are the most lethal biotoxins known to mankind. By inhibiting neurotransmitter release, BoNTs cause severe neuroparalytic disease, botulism. A series of important findings in the past 10 years which displayed the molecular targets of BoNTs and hence proposed a four-step action mechanism to explain BoNT intoxication greatly advanced the study of antibotulismic drug. In this article, we reviewed these progresses and anti-botulismic compounds found in recent years. These compounds function due to their facilitation on neurotransmitter release or to their interference on the binding, internalization, translocation, and endopeptidase activity of the toxins. Toosendanin is a triterpenoid derivative extracted from a digestive tract-parasiticide in Chinese traditional medicine. Chinese scientists have found that the compound is a selective prejunctional blocker. In spite of sharing some similar action with BoNT, toosendanin can protect botulism animals that have been administrated with lethal doses of BoNT/A or BoNT/B for several hours from death and make them restore normal activity. The neuromuscular junction preparations isolated from the rats that have been injected with toosendanin tolerate BoNT/A challenge. Toosendanin seems to have no effect on endopeptidase activity of BoNT, but blocks the toxin approach to its enzymatic substrate.

关键词： toosendanin botulinum toxins botulism synaptic transmission neurotransmitter triterpene Chinese traditional medicine

Expression of Bcl-2 inhibited Fas-mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells

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Cell Research 2000年第3期10卷 233-242页

作者： CHANG YUN CHAO, YONG HUA XU (shanghai institute of Cell Biology, Chinese Academy of Sciences, 320 Yue-yang Road, shanghai 200031, China) shanghaiInstituteofCellBiology ChineseAcademyofSciences.

Apoptosis plays an important role in embryonic development, tissue remodeling, immune regulation and tumor regression. Two groups of molecules (Bcl-2 family and 'Death factor' family) are involved in regulating apoptosis. In order to know about the effect of Bcl-2 on apoptosis induced by Fas, a typical member of 'Death factor' family, the transfection experiments with expression vectors pcDNA3-fl and pcDNA3-bcl-2 were performed in BEL-7404 cells, a human hepatocellular carcinoma cell line which expresses endogenous Fas, but not FasL and Bcl-2. The data showed that the expression of FasL in pcDNA3-fl transfected hepatoma cells obviously induced the apoptosis of the cells. However, the overexpression of Bcl-2 in pcDNA3-bcl-2 transfected 7404/b-16 cells counteracted pcDNA3-fl transient transfection mediated apoptosis. Further study by cotransfection experiments indicated that Bid but not Bax (both were pro-apoptotic proteins of Bcl-2 family) blocked the inhibitory effect of Bcl-2 on Fas-mediated apoptosis. These results suggested that Fas-mediated apoptosis in human hepatoma cells is possibly regulated by Bcl-2 family proteins via mitochondria pathway.

关键词： Apoptosis FasL Bcl-2 Bid Bax human hepa- tocellular carcinoma cells.

Cloning and analysing of 5' flanking region of Xenopus organizer gene noggin

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Cell Research 1999年第3期9卷 209-216页

作者： TAO QIN HUA JING YANG WEN YAN MEI XlNGENG XIAO YAN DING(shanghai Institute of Cell Biology, CAS, shanghai 200031,China) 1shanghai Institute of Cell Biology CAS Shanghai 200031 China 1shanghai Institute of Cell Biology CAS Shanghai 200031 China 1shanghai Institute of Cell Biology CAS Shanghai 200031 China 1shanghai Institute of Cell Biology CAS Shanghai 200031 China 1shanghai Institute of Cell Biology CAS Shanghai 200031 China

Xenopus organizer specific gene Noggin possessesnearly all the characterestic properties of the action of or-ganizer to specify the embryonic body axis. To analyzehow the maternal inherited factors cofltrol its expressionpattern, we cloned the 5' regulatory region of noggn *** 1.5 kb upstream sequense could direct reporter geneto express in vivo and data from deletion analysis indi-cated that a 229 base pair fragmet is essential for acti-vating noggn expression. We further demonstrated thatthe response elements within this regulatory region wereindeed under the control of growth factor activin and Wntsignaling pathway components.

关键词： Xenopus organizer specific gene noggin regulatory region

An Efficient Cleavage of 1,3-Dioxolane Using ZnCl_2/THF/H_2O

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Chinese Chemical Letters 2000年第2期11卷 95-96页

作者： Li Gong OU Dong Lu BAI (shanghai Institute of Materia Medica, the Chinese Academy of sciences 294 Taiyuan Road, shanghai, 200031) Chinese Acad Sci Shanghai Inst Mat Med Shanghai 200031 Peoples R China

A new. mild and efficient method for the cleavage of 1,3-dioxolane is reported.

关键词： 1,3-Dioxolane ketal deprotection

维普期刊数据库

A patch-clamp study on human sperm Cl^- channel reassembled into giant liposome

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Asian Journal of Andrology 2001年第3期3卷 185-191页

作者： Jun-Ping BAI Yu-Liang SHI Key Laboratory of Neurobiology,Intitute of Physiology,shanghai Institutes for Biological Sciences,Chinese Academy of Science, shanghai 200031,China Key Laboratory of Neurobiology Institute of Physiology Shanghai Institutes for Biological SciencesChinese Academy of Sciences Shanghai 200031 China

Aim: To record the single-channel currents and characterize the electrophysiological properties of the Cl^- channels inhuman sperm membrane. Methods: The membrane proteins extracted from the human sperm were reassembled intoliposome bilayer, and the liposomes were fused into giant liposomes with a diameter more than 10μm by dehydration-rehydration procedure. The giant liposomes were used to study the Cl^- channel activities by patch-clamp ***: By patch clamping the giant liposome in an asymmetric NMDG (N-methyl-D-glucamine)-Cl (bath 100//pipette 200 mmol/L) solution system, three kinds of single-channel events with unit conductances of (74.1 ± 8.3) pS,(117.0±5.7) pS and (144.7±4.5) pS, respectively, were detected. Their activities were voltage-dependent and allwere blocked by SITS (4-acetamido-4'-isothiocyanato-stilbene-2', 2'-disulfonic acid) in a concentration-dependentmanner. By constructing the open and close dwell time distribution histograms and then fitting them with exponentialfunction, two time constants were obtained in both the open and the close states. The burst activity and conductancesubstate of the channels were observed. Conclusion; There exist three kinds of Cl^- channels with different conduc-tance in human sperm membrane at least. (Asian J Androl 2001 Sep; 3: 185 - 191)

关键词： Cl^-channel giant liposome patch clamp human sperm membrane ion channel reassembly

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Regulation of eukaryotic DNA replication and nuclear structure

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Cell Research 1999年第3期9卷 163-170页

作者： WU JIA RUI(shanghai Institute of Biochemistry, Chinese Academy ofSciences shanghai 200031, China)e-mail: wwir@sunm.shcnc. ac. cn 1shanghai Institute of Biochemistry Chinese Academy of Sciences Shanghai 200031 China e-mail: wujr@***

In eukaryote, nuclear structure is a key component forthe functions of eukaryotic cells. More and more evidencesshow that the nuclear structure plays important role in re-gulating DNA replication. The nuclear structure providesa physical barrier for the replication licensing, participatesin the decision where DNA replication initiates, and orga-nizes replication proteins as replication factory for DNAreplication. Through these works, new concepts on theregulation of DNA replication have emerged, which willbe discussed in this minireview.

关键词： DNA replication nuclear structure replication licensing replication origin replication factory

Construction of normal human IgE phage antibody library and the screening of the anti-trichosanthin IgE

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Cell Research 1996年第1期6卷 1-9页

作者： ZAN HONG MING YEH (shanghai Institute of Cell Biology, Chinese Academy of Sciences, shanghai 200031, China.) shanghai Institute of Cell Biology Chinese Academy of Sciences Shanghai 200031 China.Received 3April1996 Revised 11June1996 Accepted 12June1996

Allergen specific IgE response is the major cause of immediate hypersensitivity. However the number of IgEproducing B cells and the amount of IgE, especially the specific IgE, are so low, it greatly impedes the study of the allergic-specifc antibody responses. Here we report the construction of a normal human IgE combinatorial library The repertoire of IgE VH genes and of K genes were separately amplified from normal human peripheral blood lymphocytes through RT-PCR, and were then constructed to form the phage surface display human Fab(IgEVH) library. A plant protein allergen, trichosanthin(TCS), was used to affinity-enrich and to screen the anti-TCS phage HuFab clones from the library. Human IgE(Fab) to TCS were detected.

关键词： Phage antibody library human IgE(Fab) human anti-trichosanthin IgE