mad-overexpression down regulates the malignant growth and p53 mediated apoptosis in human hepatocellular carcinoma BEL-7404 cells

作     者：ZHAO HUA YONG HUA XU (Shanghai Institute of Cell Biology, Chinese Academy of Sciences, 320 yue-yang Road, Shanghai 200031, China) 

作者机构：1Shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 1Shanghai Institute of Cell Biology Chinese Academy of Sciences 320 Yueyang Road Shanghai 200031 China 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：1999年第9卷第1期

页      面：51-59页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：Mad c-Myc cell growth apoptosis human hepatoma cells 

摘      要：Mad protein has been shown as an antagonist of cMyc protein in some cell lines. The effect of Mad protein to the malignant phenotype of human hepatoma BEL7404 cell line was investigated experimentally. An eukarryotic vector pCDNA Ⅲ containing full ORF fragmentof mad cDNA was transfected into targeted cells. Under G418 selection, stable Mad-overexpressed cells were *** on the effect of Mad over-expression in cell proliferation and cell cycle revealed that cell morphology of the Mad-overexpressed BEL-7404-M1 cells was significantly different from the parent and control vector transfected cells. DNA synthesis, cell proliferation and anchorage-independent growth in soft-agar of the madtransfected cel1s were partially inhibited in comparison to control *** Cytometry analysis indicated that mad over-expression might block more transfectant cells at G0/G1 phase, resulting in the retardation of cell proliferation. RT-PCR detected a marked inhibition of the expression of cdc25A, an important regulator gene of G0/G1to S phase in cell cycle. It was also found that Mad protein overexpression could greatly suppress p53-mediated apoptosis in BEL-7404-M1 cells in the absence of ***, Mad proteins may function as a negative regulator antagonizing c-Myc activity in the control of cell growth and apoptosis in human hepatocellular carcinoma BEL7404 *** and regulation of cell growth and apoptosis