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RNA silencing:From discovery and elucidation to application and perspectives

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Journal of Integrative Plant Biology 2022年第2期64卷 476-498页

作者： Jian-Hua Zhao hui-shan Guo State Key Laboratory of Plant Genomics Institute of Microbiologythe Chinese Academy of SciencesBeijing100101 China CAS Center for Excellence in Biotic Interactions University of the Chinese Academy of SciencesBeijing100049 China

RNA silencing(or RNA interference,RNAi)is a conserved mechanism for regulating gene expression in *** discovery of natural trans-kingdom RNAi indicated that small RNAs act as signaling molecules and enable communication between organisms in different *** phenomenon and potential mechanisms of trans-kingdom RNAi are among the most exciting research *** better understand trans-kingdom RNAi,we review the history of the discovery and elucidation of RNAi *** on canonical RNAi mechanisms,we summarize the major points of divergence around RNAi pathways in the main eukaryotes’kingdoms,including plants,animals,and *** review the representative incidents associated with the mechanisms and applications of trans-kingdom RNAi in crop protection,and discuss the critical factors that should be considered to develop successful trans-kingdom RNAi-based crop protection strategies.

关键词： crop protection gene expression RNA silencing small RNAs trans-kingdom RNAi

Using 3D bioprinting to produce mini-brain

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Neural Regeneration Research 2017年第10期12卷 1595-1596页

作者： Hao-Wei Han shan-hui Hsu Institute of Polymer Science and Engineering National Taiwan UniversityTaipeiTaiwanChina Center of Tissue Engineering and 3D printing National Taiwan UniversityTaipeiTaiwanChina Institute of Cellular and System Medicine National Health Research InstitutesMiaoliTaiwanChina

Recent progresses in three-dimensional （3D） bioprinting technology accelerate the coming of the era of personalized medicine. With vari- ous printing approaches and materials developed, 3D bioprinting may have a broad range of medical applications, including the fabrication of delicate tissues/organs/or the clinical use in the future or for the es- tablishment of tissues in disease models. The principal advantages of 3D bioprinting are personalized design and precise fabrication, which are of critical importance for tissue engineering. To date, several types of biomimetic tissues, such as cartilage, skin, and vascular tissues have been fabricated by 3D bioprinting （Liaw and Guvendiren, 2017）.

关键词： Using 3D bioprinting to produce mini-brain ADSCs

Influence of Incomplete Soil Plugs on Bearing Capacities of Bucket Foundations in Clay

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China Ocean Engineering 2024年第1期38卷 144-155页

作者： LI hui-shan LIU Run YANG Xu LIAN Ji-jian State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation Tianjin UniversityTianjin 300072China School of Civil Engineering Tianjin UniversityTianjin 300072China Institute of Marine Energy and Intelligent Construction Tianjin University of TechnologyTianjin 300384China

Due to the uneven seabed and heaving of soil during pumping,incomplete soil plugs may occur during the installation of bucket foundations,and the impacts on the bearing capacities of bucket foundations need to be *** this paper,the contact ratio(the ratio of the top diameter of the soil plug to the diameter of the bucket)and the soil plug ratio(the ratio of the soil heave height to the skirt height)are defined to describe the shape and size of the incomplete soil ***,finite element models are established to investigate the bearing capacities of bucket foundations with incomplete soil plugs and the influences of the contact ratios,and the soil plug ratios on the bearing capacities are *** results show that the vertical bearing capacity of bucket foundations in homogeneous soil continuously improves with the increase of the contact ***,in normally consolidated soil,the vertical bearing capacity barely changes when the contact ratio is smaller than 0.75,while the bearing capacity suddenly increases when the contact ratio increases to 1 due to the change of failure *** contact ratio hardly affects the horizontal bearing capacity of bucket ***,the moment bearing capacity improves with the increase of the contact ratio for small aspect ratios,but hardly varies with increasing contact ratio for aspect ratios larger than ***,the reduction coefficient method is proposed based on this analysis to calculate the bearing capacities of bucket foundations considering the influence of incomplete soil *** comparison results show that the proposed reduction coefficient method can be used to evaluate the influences of incomplete soil plug on the bearing capacities of bucket foundations.

关键词： bucket foundation incomplete soil plug uniaxial bearing capacity contact ratio soil plug ratio

Finns-Kingdom RNA Silencing in Plant-Fungal Pathogen Interactions

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Molecular Plant 2018年第2期11卷 235-244页

作者： Chenlei Hua Jian-Hua Zhao hui-shan Guo State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences 100101 Beijing China College of Life Science University of the Chinese Academy of Sciences 100049 Beijing China

Fungal pathogens represent a major group of plant invaders that are the causative agents of many notorious plant diseases. Large quantities of RNAs, especially small RNAs involved in gene silencing, have been found to transmit bidirectionally between fungal pathogens and their hosts. Although host-induced gene silencing （HIGS） technology has been developed and applied to protect crops from fungal infections, the mechanisms of RNA transmission, especially small RNAs regulating trans- kingdom RNA silencing in plant immunity, are largely unknown. In this review, we summarize and discuss recent important findings regarding trans-kingdom sRNAs and RNA silencing in plant-fungal pathogen interactions compared with the well-known RNAi mechanisms in plants and fungi. We focus on the interactions between plant and fungal pathogens with broad hosts, represented by the vascular pathogen Verticillium dahliae and non-vascular pathogen Botrytis cinerea, and discuss the known instances of natural RNAi transmission between fungal pathogens and host plants. Given that HIGS has been developed and recently applied in controlling Verticillium wilt diseases, we propose an ideal research system exploiting plant vasculature-Verticillium interaction to further study trans-kingdom RNA silencing.

关键词： fungal pathogens HIGS microRNA trans-kingdom RNAi small RNA Verticillium dahliae

Synthesis of TP3 FragmentviaOne Pot Strategy and Its Immune Regulatory Activity

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维普期刊数据库

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Chemical Research in Chinese Universities 2005年第5期21卷 566-568页

作者： WANG Li-feng CHEN Jie shan hui-jie LI Wei Laboratory of Research on Biological Macromolecules College of Life Science Jilin University Changchun 130021 P. R. China

We have modified the previously described one-pot peptide synthesis method. The modified method has been successfully applied to the synthesis of TP3. Furthermore, the immune regulatory activity of TP3 has been characterized. The results show that the modified one-pot method can be used to synthesize the biological active peptide with the advantages of low cost and high productivity. Moreover, TP3 has a higher immune regulatory activity than TP5.

关键词： TP3 One-pot synthesis strategy Activity assay Immune regulatory activity

IBM1-dependent H3K9 demethylation enables self-silencing of an exogenous silencer for the non-cell autonomous silencing of an endogenous target gene

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Journal of Genetics and Genomics 2019年第3期46卷 149-153页

作者： Xiaolan Liu Jianhua Zhao hui-shan Guo State Key Laboratory of Plant Genomics Institute of MicrobiologyChinese Academy of Sciences College of Life Sciences University of the Chinese Academy of Sciences

RNA silencing(RNAi)is a nucleotide sequence-specific process that results in blockage of gene expression(Baulcombe,2004,2005;Gunter Meister,2004;Vaucheret,2006;Chinnusamy and Zhu,2009;Heo and Kim,2009;Matzke et al.,2009;Simon and Meyers,2011).In plants,post-transcriptional gene silencing(PTGS)occurs in the cytoplasm that is induced by small RNAs(sRNAs),which are the products of double-stranded RNAs(dsRNAs)processed by Dicer-like ribonucleases and achieve specificity through base pairing with targeted RNA sequences(Carmell and Hannon,2004;Gunter Meister,2004;Gasciolli et al.,2005).In an alternative pathway occurring in the nucleus,sRNAs corresponding to promoter sequences direct the silencing machinery to block the transcription of homologous promoters(transcriptional gene silencing,TGS).This process requires 24-nt sRNAs for de novo DNA methylation,a process known as RNA-directed DNA methylation(RdDM)(James *** and Jacobsen,2002;Matzke and Birchler,2005;Matzke et al.,2007;Zilberman et al.,2007;Wierzbicki et al.,200&Zilberman,2008).Histone modifications also play an important role in the establishment and maintenance of DNA methylation(Zuzana Jasencakova,2003;Ooi et al.,2007;Cedar and Bergman,2009;Law and Jacobsen,2010).In plants,it has been shown that the transcription of exogenous transgene transcribing inverted-repeat(exo-/R)sequences produces dsRNAs,triggering exo-//?PTGS that is negatively autoregulated through methylation spreading/transitive *** transitive silencing「einforces the self-silencing of exo-//?and leads to reduced exo-//?PTGS and exo-/R-derived sRNA ***-/R-derived sRNAs function as mobile signals to trigger sRNA-mediated non-cell autonomous silencing of an endogenous homologous target gene(endo-gene)(Dong et al.,2011).It remains unknown whether histone modifications play a role in the exo-IR-triggered endo-gene silencing.

关键词： RNA silencing(RNAi) sequence-specific process modifications play

Cathodic behavior of molten CaCl_2-CaO and CaCl_2-NaCl-CaO

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International Journal of Minerals,Metallurgy and Materials 2010年第6期17卷 791-794页

作者： Shu-lan Wang Wei Wang Shi-chao Li shan-hui Cao Department of Chemistry Northeastem University Shenyang 110004 China

The cathodic behavior of molten CaCl2, CaCl2-CaO and equimolar CaCl2-NaCl-CaO was studied by cyclic voltammograms and constant potential polarization at temperatures of 1123 to 1173 K on molybdenum and titanium electrodes. The diffusion coefficient of Ca2＋（CaO） in molten CaCl2-CaO was calculated from the linear relationship between the square root of scan rate and the peak current density. The deposition potentials and the potential temperature coefficient of CaO in molten CaCl2-0.5mol%CaO and CaCl2-NaCl-0.5mol%CaO were also obtained from their cyclic voltammograms. The result shows that CaO is more easily reduced than CaCl2. The addition of NaCl in molten CaCl2-CaO induces the underpotential electrodeposition of CaO.

关键词： molten salts calcium oxide cyclic voltammetry polarization

Synthesis of CdTe/SiO_2 Composites and Their Fluorescence Properties

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Chemical Research in Chinese Universities 2011年第4期27卷 540-542页

作者： WANG Mei-ping LIN Jin-hui shan Chuan-sheng College of Materials and Chemistry ＆ Chemical Engineering Chengdu University of Technology Chengdu 610059 P. R. China

Thioglycolic acid（TGA）-stabilized CdTe nanocrystals（NCs） were prepared with sodium tellurite as tellurium source,which avoids the cumbersome processes associated with H2Te or NaHTe *** CdTe/SiO2 composites were synthesized by a sol-gel method without the exchange of surface *** phase structure of CdTe NCs was investigated by X-ray *** comparison,some characterizations were done for both the CdTe NCs and the *** NCs and CdTe/SiO2 composites were characterized with TEM,digital camera and fluorescence *** stability of CdTe NCs and the composites were investigated in phosphate-buffered saline（PBS） buffer and the fluorescent properties of the composites were discussed in detail.

关键词： CdTe nanocrystal Sodium tellurite CdTe/SiO2

A rotary ultrasonic motor using radial bending mode of ring with nested PZT excitation

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2012年第3期13卷 189-196页

作者： Ying-xiang LIU Jun-kao LIU Wei-shan CHEN Xiao-hui YANG State Key Laboratory of Robotics and System Harbin Institute of Technology Horbin 150001 China

This study presents and verifies a new idea for constructing a rotary traveling wave ultrasonic motor （USM） that uses the radial bending mode of a ring. In the new design, 20 trapezoid cross section slots are cut symmetrically in the outer surface of a thick duralumin alloy ring, where 20 PZT stacks are nested. In each slot, two wedging blocks are set between the PZT stack and the two sides of the slot respectively to apply preloading on the PZT ceramics. Two radial bending modes of the stator that have a phase difference of a quarter wavelength on space are generated by using the d33 operating mode of the PZT elements, and then a flexural traveling wave is formed by the superimposing of two standing waves whose amplitudes are equal and phases are different by 90~ temporally. Two conical rotors are pressed to each end of the ring type stator by a coiled spring. The finite element method （FEM） simulation is developed to validate the feasibility of the proposed motor. The maximal speed and torque of the prototype are tested to be 126 r/rain and 0.8 N＇m, respectively.

关键词： Rotary ultrasonic motor Radial bending mode Traveling wave Wedging block clamped structure