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Prevalence of plasmid mediated bla_(TEM-1) and bla_(CTX-M-15) type extended spectrum beta-lactamases in patients with sepsis

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Asian Pacific Journal of Tropical Medicine 2012年第2期5卷 98-102页

作者： shahzad F Haque Saeedut-Z ali Mohammed TP Asad U Khan Department of Medicine J.N.Medical CollegeAligarhIndia Interdisciplinary Biotechnology Unit AMUAligarh Muslim UniversityAligarhIndia Department of Medicine J.N.Medical College.AligarhIndia

Objective:To characterize the bacterial pathogens in patients having gram negative septicaemia. Further,to evaluate the antimicrobial resistance and underlying molecular mechanisms in these ***:A total number of 70 cases of gram negative sepsis were included in this prospective,open labeled,observational *** methods for isolation and identification of bacteria were *** susceptibility and ESBL testing was performed by the standard disc diffusion *** amplification was performed to identify blaCTX-M,blaSHVand blaTEM type *** experiments were performed to show resistant marker transfer. Results:The most prevalent isolates Escherichia coli（***） 58.6%,Klebsiella Spp.32.9%and Pseudomonas 8.6%,were resistant to most of the antimicrobials including cefazolin,ceftriaxone, cefuroxime,ampicillin and co-trimoxazole but sensitive to imipenem and *** and MBL production was seen 7.3%and 12.2%of *** isolates *** isoaltes were found to have blaCTX-M-15 and two of them also showed blaTEM-1 type ***, none of them showed blaSHV.Conjugation experiments using J-53 cells confirmed these resistant markers as plasmid ***:This work highlights the molecular epidemiology of escalating antimicrobial resistance and likely switch over of blaCTX-M-15 type extended spectrum beta-lactamases by blaTEM type ESBLs in ***,the antimicrobial resistance by horizontal gene transfer was predominant among Enterobacteraceae in the community setting.

关键词： Gram negative sepsis ESBL Antimicrobial resistance

Phosphorus-Mobilizing Rhizobacterial Strain Bacillus cereus GS6 Improves Symbiotic Efficiency of Soybean on an Aridisol Amended with Phosphorus-Enriched Compost

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Pedosphere 2017年第6期27卷 1049-1061页

作者： Muhammad Saleem ARIF Muhammad RIAZ Sher Muhammad shahzad Tahira YASMEEN Shafaqat ali Muhammad Javed AKHTAR Department of Environmental Sciences and Engineering Government College University Faisalabad Department of Soil&Environmental Sciences University College of AgricultureUniversity of Sargodha Institute of Soil&Environmental Science University of Agriculture Faisalabad

Legume plants are an essential component of sustainable farming ***(P) deficiency is a significant constraint for legume production, especially in nutrient-poor soils of arid and semi-arid *** the present study, we conducted a pot experiment to evaluate the effects of a phosphorus-mobilizing plant-growth promoting rhizobacterial strain Bacillus cereus GS6, either alone or combined with phosphate-enriched compost(PEC) on the symbiotic(nodulation-N_2 fixation) performance of soybean(Glycine max(L.) Merr.) on an *** PEC was produced by composting food waste with addition of single super *** bacterial strain *** GS6 showed considerable potential for P solubilization and mobilization by releasing carboxylates in insoluble P(rock phosphate)-enriched *** of *** GS6 in combination with PEC application significantly improved nodulation and nodule N_2 fixation *** to the control(without *** GS6 and PEC), the combined application of *** GS6 with PEC resulted in significantly higher accumulation of nitrogen(N), P, and potassium(K) in grain, shoot, and *** N:P and P:K ratios in nodules were significantly altered by the application of PEC and *** GS6, which reflected the important roles of P and K in symbiotic performance of *** combined application of PEC and *** GS6 also significantly increased the soil dehydrogenase and phosphomonoesterase activities, as well as the soil available N, P, and K *** positive relationships were found between soil organic carbon(C) content, dehydrogenase and phosphomonoesterase activities, and available N, P, and K *** study suggests that inoculation of P-mobilizing rhizobacteria, such as *** GS6, in combination with PEC application might enhance legume productivity by improving nodulation and nodule N_2 fixation efficiency.

关键词： arid and semi-arid regions carboxylate N2 fixation nodulation nutrient availability nutrient-poor soils plant growth-promoting rhizobacteria (PGPR) soil enzymes

Effect of radiation on compressibility of hot dense sodium and iron plasma using improved screened hydrogenic model with𝑙splitting

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Chinese Physics B 2021年第3期30卷 225-236页

作者： Amjad ali G Shabbir Naz Rukhsana Kouser Ghazala Tasneem M Saleem shahzad Aman-ur-Rehman M H Nasim Department of Physics and Applied Mathematics Pakistan Institute of Engineering and Applied SciencesIslamabad 45650Pakistan Department of Nuclear Engineering Pakistan Institute of Engineering and Applied SciencesIslamabad 45650Pakistan

High pressure investigations of matter involve the study of strong shock wave dynamics within the materials which gives rise to many thermal effects leading to dissociation of molecules,ionization of atoms,and radiation emission,*** response of materials experiencing a strong shock can be determined by its shock Hugoniot calculations which are frequently applied in numerical and experimental studies in inertial confinement fusion,laboratory astrophysical plasma,*** studies involve high energy density plasmas in which the radiation plays an important role in determining the energy deposition and maximum compressibility achieved by the shock within *** this study,we present an investigation for the effect of radiation pressure on the maximum compressibility of the material using shock Hugoniot *** shock Hugoniot calculations,an equation of state(EOS)is developed in which electronic contributions for EOS calculations are taken from an improved screened hydrogenic model with−l splitting(I-SHML)[High Energy Density Physics(2018)2648]under local thermodynamic equilibrium(LTE)*** thermal ionic part calculations are adopted from the state of the art Cowan model while the cold ionic contributions are adopted from the scaled binding energy *** Shock Hugoniot calculations are carried out for sodium and iron plasmas and our calculated results show excellent agreement with published results obtained by using either sophisticated self-consistent models or the first principle study.

关键词： self-consistent-field methods thermodynamics of plasmas average atom model shock Hugoniot calculation

Ion population fraction calculations using improved screened hydrogenic model with l-splitting

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Chinese Physics B 2018年第10期27卷 424-432页

作者： Amjad ali G Shabbir Naz Rukhsana Kouser Ghazala Tasneem M Saleem shahzad Aman-ur-Rehman M H Nasim Department of Physics and Applied Mathematics Pakistan Institute of Engineeringand Applied Sciences Department of Nuclear Engineering Pakistan Institute of Engineeringand Applied Sciences

Ion population fraction（IPF） calculations are very important to understand the radiative spectrum emitted from the hot dense matter. IPF calculations require detailed knowledge of all the ions and correlation interactions between the electrons of an ion which are present in a plasma environment. The average atom models, e.g., screened hydrogenic model with l-splitting（SHML）, now have the capabilities for such calculations and are becoming more popular for in line plasma calculations. In our previous work [ali A, Shabbir Naz G, shahzad M S, Kouser R, Rehman A and Nasim M H 2018 High Energy Density Phys. 26 48], we have improved the continuum lowering model and included the exchange and correlation effects in SHML. This study presents the calculation of IPF using classical theory of fluctuation for our improved screened hydrogenic model with l-splitting（I-SHML） under local thermodynamic equilibrium conditions for iron and aluminum plasma over a wide range of densities and temperatures. We have compared our results with other models and have found a very good agreement among them.

关键词： self-consistent average atom model average ionization state ion population

Neural Machine Translation Models with Attention-Based Dropout Layer

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Computers, Materials & Continua 2023年第5期75卷 2981-3009页

作者： Huma Israr Safdar Abbas Khan Muhammad ali Tahir Muhammad Khuram shahzad Muneer Ahmad Jasni Mohamad Zain School of Electrical Engineering and Computer Science(SEECS) National University of Science and TechnologyIslamabadPakistan Institute for Big Data Analytics and Artificial Intelligence(IBDAAI) Kompleks Al-KhawarizmiUniversiti Teknologi MARA40450Shah AlamSelangorMalaysia

In bilingual translation,attention-based Neural Machine Translation(NMT)models are used to achieve synchrony between input and output sequences and the notion of *** model has obtained state-of-the-art performance for several language ***,there has been little work exploring useful architectures for Urdu-to-English machine *** conducted extensive Urdu-to-English translation experiments using Long short-term memory(LSTM)/Bidirectional recurrent neural networks(Bi-RNN)/Statistical recurrent unit(SRU)/Gated recurrent unit(GRU)/Convolutional neural network(CNN)and *** results show that Bi-RNN and LSTM with attention mechanism trained iteratively,with a scalable data set,make precise predictions on unseen *** trained models yielded competitive results by achieving 62.6%and 61%accuracy and 49.67 and 47.14 BLEU scores,*** a qualitative perspective,the translation of the test sets was examined manually,and it was observed that trained models tend to produce repetitive output more *** attention score produced by Bi-RNN and LSTM produced clear alignment,while GRU showed incorrect translation for words,poor alignment and lack of a clear ***,we considered refining the attention-based models by defining an additional attention-based dropout *** dropout fixes alignment errors and minimizes translation errors at the word *** empirical demonstration and comparison with their counterparts,we found improvement in the quality of the resulting translation system and a decrease in the perplexity and over-translation *** ability of the proposed model was evaluated using Arabic-English and Persian-English datasets as *** empirically concluded that adding an attention-based dropout layer helps improve GRU,SRU,and Transformer translation and is considerably more efficient in translation quality and speed.

关键词： Natural language processing neural machine translation word embedding attention perplexity selective dropout regularization Urdu Persian Arabic BLEU

High HbA1c is associated with higher risk of ischaemic stroke in Pakistani population without diabetes

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Stroke & Vascular Neurology 2016年第3期1卷 133-139页

作者： ali Zohair Nomani Sumaira Nabi shahzad Ahmed Mansoor Iqbal Haris Majid Rajput Suhail Rao Department of Neurology Pakistan institute of Medical SciencesIslamabadPakistan

Context:The role of glycated haemoglobin(HbA1c)in the prediction of ischaemic stroke in individuals without diabetes is ***:We performed a study to analyse the role of HbA1c in the risk prediction of ischaemic stroke in Pakistani population without *** further studied the difference between HbA1c values of individuals with diabetes and without diabetes with *** and design:Single centre,case–*** and methods:In phase I,a total of 233 patients without diabetes with ischaemic stroke and 245 as controls were *** of HbA1c levels,lipid profiles and blood pressure recordings with ischaemic stroke was *** phase II,comparison was done between diabetics and nondiabetics with *** analysis:Comparison of the mean variables was performed with Student’s *** regression analysis with ischaemic stroke as the dependent variable was performed for phase ***:In phase I,the ischaemic stroke group had significantly higher HbA1c levels(5.9±2.9%vs 5.5±1.6%)compared with controls(p0.05).Conclusions:Higher HbA1c indicated a significantly increased risk for ischaemic *** HbA1c value above 5.6%(prediabetic range)predicted future risk of stroke and efforts to maintain glucose level within the normal range(≤5.6%)in individuals with high cardiovascular risk are important.

关键词： diabetes cholesterol cardiovascular

Multi-Modal Data Analysis Based Game Player Experience Modeling Using LSTM-DNN

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Computers, Materials & Continua 2021年第9期68卷 4087-4108页

作者： Sehar shahzad Farooq Mustansar Fiaz Irfan Mehmood ali Kashif Bashir Raheel Nawaz KyungJoong Kim Soon Ki Jung School of Computer Science and Engineering Kyungpook National UniversityDaeguKorea Faculty of Engineering&Informatics School of MediaDesign and TechnologyUniversity of BradfordBradfordBD71DPUK Department of Computing and Mathematics Manchester Metropolitan UniversityManchesterUK Department of Operations TechnologyEvents and Hospitality ManagementManchester Metropolitan UniversityUK Institute of Integrated Technology Gwangju Institute of Science and TechnologyKorea

Game player modeling is a paradigm of computational models to exploit players’behavior and experience using game and player *** modeling refers to descriptions of players based on frameworks of data derived from the interaction of a player’s behavior within the game as well as the player’s experience with the *** behavior focuses on dynamic and static information gathered at the time of *** experience concerns the association of the human player during gameplay,which is based on cognitive and affective physiological measurements collected from sensors mounted on the player’s body or in the player’s *** this paper,player experience modeling is studied based on the board puzzle game“Candy Crush Saga”using cognitive data of players accessed by physiological and peripheral *** Short-Term Memory-based Deep Neural Network(LSTM-DNN)is used to predict players’effective states in terms of valence,arousal,dominance,and liking by employing the concept of transfer *** learning focuses on gaining knowledge while solving one problem and using the same knowledge to solve different but related *** homogeneous transfer learning approach has not been implemented in the game domain before,and this novel study opens a new research area for the game industry where the main challenge is predicting the significance of innovative games for entertainment and players’*** not only from a player’s point of view,it is also a benchmark study for game developers who have been facing problems of“cold start”for innovative games that strengthen the game industrial economy.

关键词： Game player modeling experience modeling player analytics deep learning LSTM game play data Candy Crush Saga

RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato

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Science Bulletin 2017年第7期62卷 476-485页

作者： Mohamed Ewas Yangqiang Gao Farhan ali Elsayed M. Nishawy Raheel shahzad Hizar Subthain Mohamed Amar Cathie Martin Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) College of Life Sdence and Technology Huazhong Agricultural University Wuhan 430070 China Egyptian Deserts Gene Bank (EDGB) Genetic Resources Department Cairo Egypt Deserts Research Center (DRC) Cairo Egypt John lnnes Centre Norwich Research Park Colney Norwich NR4 7UH UK

Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human *** previous study reported that manipulation of Sl MX1 gene enhanced carotenoids accumulation and drought resistance in ***,RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of Sl MX1 involved in stress resistance and enhanced secondary *** analysis showed that overexpression of Sl MX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic(fungus,bacteria,virus and insects) stresses in *** research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome *** addition,we reported two types of trichome which seems to be aberrant types in *** study unravels the mechanism of regulation of Sl MX1,which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.

关键词： Tomato SlMX1 Resistance Carotenoids Terpenoids Mechanism RNA Seq

A review on marine collagen:sources,extraction methods,colloids properties,and food applications

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Collagen and Leather 2024年第1期6卷 1-27页

作者： shahzad Farooq Muhammad Ijaz Ahmad Shijie Zheng Usman ali Yang Li Cui Shixiu Hui Zhang Jiaxing Institute of Future Food Jiaxing 314050China College of Biosystems Engineering and Food Science Zhejiang Key Laboratory for Agro-Food ProcessingZhejiang UniversityHangzhou 310058China

The growing interest in valorizing industrial by-products has led researchers to focus on exploring different sources and optimizing collagen extraction conditions over the past *** bovine hide,cattle bones,pork,and pig skins remain the most abundant collagen sources,there is a growing trend in the industrial utilization of collagen from non-mammalian *** review explores alternative marine collagen sourcesand summarizes emerging trends in collagen recovery from marine sources,with a particular focus on environmentally friendly ***,this review covers the colloidal structure-forming properties of marine collagens,including foam,film,gel,and emulsion *** also highlights the potential and important applications of marine collagen in various food *** on the currently reported marine sources,collagens extracted from fish,jellyfish,and sea cucumbers were found to have the highest yield and mostly comprised type-l collagen,while crustaceans and mollusks yielded lower percentages of *** extraction techniques isolate collagen based on acetic acid and pepsin treatment,but they come with drawbacks such as being time-consuming,causing sample destruction,and using ***,marine collagen extracted using conventional methods assisted with ultrasonication resulted in higher yields and strengthened the triple-stranded helical ***,an increasing number of new applications have been found in the food industry for marine collagens,such as biodegradable film-forming materials,colloid stabilizers,foaming agents,and micro-encapsulating ***,collagen is a modern foodstuff and is extensively used in the beverage,dairy,and meat industries to increase the stability,consistency,and elasticity of products.

关键词： Marine collagen Jellyfish Sea cucumber Colloid stabilizer Edible film Dairy product