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Purification and Characterization of a Low-temperature Hydroxylamine Oxidase from Heterotrophic Nitrifier Acinetobacter sp.Y16

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Biomedical and Environmental Sciences 2014年第7期27卷 515-522页

作者： ZHANG Shu Mei LI Wei Guang ZHANG Duo Ying HUANG Xiao Fei QIN Wen sha chang Qing Institute of Microbiology Heilongjiang Academy of Sciences School of Municipal and Environmental Engineering Harbin Institute of Technology State Key Laboratory of Urban Water Resource and Environment Harbin Institute of Technology Heilongjiang Academy of Sciences

Objective To purify a low-temperature hydroxylamine oxidase （HAO） from a heterotrophic nitrifying bacterium Acinetobacter sp. Y26 and investigate the enzyme property. Methods A HAO was purified by an anion-exchange and gel-filtration chromatography from strain Y16. The purity and molecular mass were determined by RP-HPLC and SDS-PAGE. The HAO activity was detected by monitoring the reduction of potassium ferricyanide using hydroxylamine as substrate and ferricyanide as electron acceptor. The partial amino acid sequence was determined by mass spectrometry. Results The low-temperature HAO with a molecular mass of 61 kDa was purified from strain Y26 by an anion-exchange and gel-filtration chromatography. The enzyme exhibited an ability to oxidize hydroxylamine in wide temperature range （4-40 ℃） in vitro using hydroxylamine as substrate and ferricyanide as electron acceptor. It was stable in the temperature range of 4 to 25 ℃ and pH range of 6.0 to 8.5 with less than 30% change in its activity. The optimal temperature and pH were 15 ℃ and 7.5, respectively. Three peptides were determined by mass spectrometry which were shown to be not identical to other reported HAOs. Conclusion This is the first study to purify a low-temperature HAO from a heterotrophic nitrifier Acinetobecter sp. It differs from other reported HAOs in molecular mass and enzyme properties. The findings of the present study have suggested that the strain Y26 passes through a hydroxylamine-oxidizing process catalyzed by a low-temperature HAO for ammonium removal.

关键词： Hydroxylamine oxidase Purification Heterotrophic nitrifier Acinetobacter sp Y16

HISTORICAL-DYNAMIC INTEGRATIVE CLASSIFICATION OF BASINOGENESIS AND ORE-FORMING BASINS

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Geotectonica et Metallogenia 1994年第Z1期18卷 1-26页

作者： CHEN Guoda (chang sha Institnte of Geutectunics, A cad emia Sinica, chang sha 410013)

Interesting classifications of basinogenesis and basins were proposed by many seientists. They classified basinogenesis and basins mainly from a single angle, either from a historical angle or from a dynamie angle. In order to more comprehensively understand them for moore effectively guidlilg prospeeting and exploration, the author integrates the two methods of analysis wilh cach other and proposes an integrative classification. According to the historieal-dynamic integrative classification, basinogenesis and basins can be divided into three types: occanic erust type. embryo-continental (transitional ) erust iype and continental crust type. Oceanie erust type call be subdivided into mobile region type (mainly tensional) and stable region type. Embryo-continental type includes pre-geosynclinal type (divisible into several mobile region types and stable region types with tensional type predoiminating among mobile region trpes) and early-geosynelinal type (mainly tensional). Continental erust type ineludes late-gcosynelinal (fold belt) type (compressional or tensional), platform type (mainly sinking and rarely tensional subsidence-aulacogen) and gcodepression (diwa) type (compressional, tensional or compressional-tensional ).

关键词： basinogenesis and ore-forming basin historical-dynamic integrative classification oceanic crust type embryo-continental (transitional) crust type continental crust type pre-geosynelinal type geosynclinal type platform type geodepression (di

基于合同网的分布式卫星系统任务优化分配研究

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宇航学报 2009年第2期30卷 815-820页

作者：高黎沙基昌国防科学技术大学信息系统与管理学院长沙410073

面向未来分布式卫星系统(DSS)自主协作运行模式,对DSS任务协作中的优化分配问题进行了分析和描述,提出了基于协商的任务优化分配体系结构,并由此给出任务组的概念,通过集覆盖理论将任务分配问题转化为集覆盖问题。从而引入合同网协议,... 详细信息

面向未来分布式卫星系统(DSS)自主协作运行模式,对DSS任务协作中的优化分配问题进行了分析和描述,提出了基于协商的任务优化分配体系结构,并由此给出任务组的概念,通过集覆盖理论将任务分配问题转化为集覆盖问题。从而引入合同网协议,以系统完成任务目标的总耗能最少为原则,加入限定卫星可回应任务数的约束后,提出了一种基于合同网的严格启发式优化分配算法对问题进行求解。同时,证明了算法搜索结果的上确界;分析了算法的收敛性和时间复杂度。该算法具有分布性,搜索空间缩减快,适合于中小规模问题的任务分配。

关键词：分布式卫星系统任务协作任务分配合同网

Nonuniform stretch processing for the range profile of a target with micro-motion

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Progress in Natural Science:Materials International 2006年第11期16卷 1205-1213页

作者： CHEN Hangyong**, JIANG Weidong, LIU Yongxiang, LI Xiang and GUO Guirong(Institute of Space Electronics Information Technology, National University of Defense Technology, chang sha 410073, China) Institute of Space Electronics Information Technology National University of Defense Technology Chang Sha 410073 China

Complex motions such as micro-motions induce complex range-Doppler coupling effect in radar imaging. Hence, it is a challenge to reconstruct a clear range profile of a target with micro-motion. Stretch processing with fine motion compensation cannot perform well while it ties to eliminate the distortion of range profile caused by Doppler effect, because it is difficult to accurately estimate each scattering center velocity during coherent processing interval. Based on nonuniform stretch processing (NSP), a new technology is proposed to decouple range, time and Doppler and then achieve range profile of target with micro-motion. The new technology uses a new synthetic wide-band waveform and has an innovative mathematic interpretation. Statistical properties of the new approach are primarily examined. Typical models of target with micro-motion for high resolution radar are presented. Monte Carlo simulations demonstrate the performance of the new technology and the simulation results confirm our expectations.

关键词： synthetic range profile micro-motion stretch processing Doppler effect.

The Psychoanalytic Perspective of the Main Characters in the American TV Series Friends

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Journal of Literature and Art Studies 2023年第3期13卷 210-214页

作者： ZHAO Mao-jin chang sha National University of Defense and Technology ChangshaHunanChina

As a recognized classic of American TV series,“Friends”tells the complex story of friendship,love between six distinctive American young *** family backgrounds and childhood experiences presented in TV dramas take account for their difference in *** the theoretical concept of psychoanalytic literature,this article attempts to reveal how the specific influence and manifestation of the family situation and parental behavior have on the character and behavior of the protagonist,as well as to further analyze how the trauma suffered in childhood functions in adult *** adults,their behavior and habits are still constrained and influenced by their childhood experiences.

关键词： psychoanalytical theory childhood family background friends

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气象海洋作战仿真环境复杂性评估及分级方法

系统工程理论与实践 2021年第7期41卷 1868-1881页

作者：常沙宋彬黎鑫国防科技大学系统工程学院长沙410073 国防科技大学气象海洋学院长沙410073

气象海洋作战仿真环境的影响评估是检验仿真环境与模拟训练难度一致性的关键问题.现有气象海洋作战仿真环境评估方法主要用于分析静态环境要素对武器装备、作战平台等的影响作用,不适用于对模拟训练中的动态复杂仿真环境进行综合评估.... 详细信息

气象海洋作战仿真环境的影响评估是检验仿真环境与模拟训练难度一致性的关键问题.现有气象海洋作战仿真环境评估方法主要用于分析静态环境要素对武器装备、作战平台等的影响作用,不适用于对模拟训练中的动态复杂仿真环境进行综合评估.为了有效评估仿真环境的复杂程度,本文首先明确了气象海洋作战仿真环境复杂度的内涵,并对复杂度评估及分级层次结构进行了分析;然后,提出了复杂度评估指标,基于矩阵型灰色关联理论,建立了环境复杂度评估模型;随后,提出了复杂度分级原则,基于灰色聚类方法,建立了环境复杂度分级模型,并对可能度函数进行了改进,从而得到动态仿真环境的复杂度等级.通过模拟训练实例对本文方法验证,表明该方法能够对动态仿真环境复杂度等级做出合理评估,有助于构建符合模拟训练难度要求的仿真环境.

关键词：模拟训练气象海洋作战仿真环境复杂度评估与分级矩阵型灰色关联灰色聚类

THE MECHANICAL RELATIONSHIP TO THE FORMATION AND DISTRIBUTION OF THEBURIED HILLS AND THE DEEP-SEATED FRACTURAL ZONE IN THE DIWA-TYPE FAULTED BASIN IN CENTRAL HEBEI PROVINCE,CHINA

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Geotectonica et Metallogenia 1994年第Z1期18卷 27-31页

作者： LIN Ge & WEI Zhouling (chang sha Institute of Geotectonics, Acad emia Sinica, chang sha, 410013)

The author proves the existence and movement of a deep-seated fraetural Zone located in the eenter zone of the diwa-type faulted basin in central Hebei Province. This deep-seated fraeturai zone tending in NNE direction is a structural effect of the mtodle East Asin Grustobody in the Mexozoic-Cenozoic. This paper will diseuss the formation, evolution and the meehanism of the deep-seated fraetural zone, faulted basin and the buried hills as well as their relationships. The uthor expounds that the deep geological process is the major factor of the structural effect.

关键词： deep-seated fraetural zone buried hill East Asia Crustobody the basin in central Hebei Province

THE EVOLUTION OF THE LANKAO-LIAOCHENG FRACTURAL ZONE AND ITS OIL-GAS ACCUMULATION

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Geotectonica et Metallogenia 1994年第Z1期18卷 33-37页

作者： WEI Zhouling LIN Ge and QIN Qingxiang (chang sha Institute of Geotectonies, A cad emia Siniea, chang sha, 410013)

The Lankao-Liaocheng Fractural Zone is a large-seade NNE-trending struetural zone in the North China Crustoblock. Dated from the Late Arehean-Early Proterozoie, it is stil1 active now. Its nature varied with time in its developing process. It has became a tensile ultralithospherie fraetural zone at present or in diwa residual-mobility period. Some cenozoic oil-gas-bearing basins are distributed along the fraetural zone which fomed an important oil-gas accumulating belt in the North China Ctustoblock.

关键词： Lankao-Liaocheng Fractural Zone evolution oil-gas accumulation

INVESTIGATION OF THE PALEOGEOTHERMAL TECTONIC HISTORY ON DONGPU DEPRESSION AND OIL/GAS FORMATION BY FISSION-TRACK METHOD

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Geotectonica et Metallogenia 1994年第Z1期18卷 38-50页

作者： WANG Lu (Cnang sha Institute of Geoteetonies, Acad emia Sinica, chang sha, 410013)

Dongpu depression is a fault basin at residual-mobility period of Diwa stage, it developed on the strata of the Mesozoie and Pre-Mesozoie. It is one of the important oil/gas- bearing basin during Cenozoie era along the East China. The strueture in Dongpu geodepression is very complex. There exists a strueture pattern with east- and west-depression belts and one central swell belt, it is divided into the southern- and northern-division by Gaopingji (orMeng Ju ) -Xieheng fault.

关键词： GAS INVESTIGATION OF THE PALEOGEOTHERMAL TECTONIC HISTORY ON DONGPU DEPRESSION AND OIL/GAS FORMATION BY FISSION-TRACK METHOD Pb