Response of ammonia-oxidizing archaea to heavy metal contamination in freshwater sediment

作     者：Ningning Li Yao Chen Zhengke Zhang Sha Chang Dawei Huang Sili Chen Qingwei Guo Shuguang Xie Yongxin Bing 

作者机构：State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering Peking University South China Institute of Environmental Sciences (SCIES) Ministry of Environment Protection (MEP) 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2019年第31卷第3期

页      面：392-399页

核心收录：

学科分类：07[理学] 08[工学] 09[农学] 0815[工学-水利工程] 0903[农学-农业资源与环境] 0713[理学-生态学] 

基　　金：supported by Guangdong Province Science and Technology Project(No.2016B020240007) the Basic Scientific Research Business of Central Level Public Welfare Scientific Research Institution(No.PM-zx703-201803-070) 

主　　题：Ammonia-oxidizing archaea Freshwater sediment Heavy metal Nitrosopumilus 

摘      要：It has been well-documented that the distribution of ammonia-oxidizing bacteria(AOB) and archaea(AOA) in soils can be affected by heavy metal contamination, whereas information about the impact of heavy metal on these ammonia-oxidizing microorganisms in freshwater sediment is still lacking. The present study explored the change of sediment ammonia-oxidizing microorganisms in a freshwater reservoir after being accidentally contaminated by industrial discharge containing high levels of metals. Bacterial amoA gene was found to be below the quantitative PCR detection and was not successfully amplified by conventional PCR. The number of archaeal amoA gene in reservoir sediments were 9.62 × 10~2–1.35 × 10~7 copies per gram dry sediment. AOA abundance continuously decreased, and AOA richness, diversity and community structure also considerably varied with time. Therefore, heavy metal pollution could have a profound impact on freshwater sediment AOA community. This work could expand our knowledge of the effect of heavy metal contamination on nitrification in natural ecosystems.