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Long-term adenosine A1 receptor activation-induced sortilin expression promotes α-synuclein upregulation in dopaminergic neurons

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Neural Regeneration Research 2020年第4期15卷 712-723页

作者： Yun-Cheng lv An-Bo Gao Jing Yang Li-Yuan Zhong Bo Jia Shu-Hui Ouyang Le Gui Tian-Hong Peng sha Sun Francisco S.Cayabyab Clinical Anatomy&Reproductive Medicine Application Institute Hengyang Medical CollegeUniversity of South ChinaHengyangHunan ProvinceChina Department of Surgery College of MedicineUniversity of SaskatchewanSaskatoonCanada Institute of Pharmacy and Pharmacology School of Pharmaceutical ScienceUniversity of South ChinaHengyangHunan ProvinceChina Department of Metabolism&Endocrinology the First Affiliated HospitalHengyang Medical CollegeUniversity of South ChinaHengyangHunan ProvinceChina

Prolonged activation of adenosine A1 receptor likely leads to damage of dopaminergic neurons and subsequent development of neurodegenerative ***,the pathogenesis underlying long-term adenosine A1 receptor activation-induced neurodegeneration remains *** this study,rats were intraperitoneally injected with 5 mg/kg of the adenosine A1 receptor agonist N6-cyclopentyladenosine(CPA)for five *** mobility of rats was evaluated by forced swimming test,while their cognitive capabilities were evaluated by Y-maze *** of sortilin,α-synuclein,p-JUN,and c-JUN proteins in the substantia nigra were detected by western blot *** addition,immunofluorescence staining of sortilin andα-synuclein was performed to detect expression in the substantia *** results showed that,compared with adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(5 mg/kg)+CPA co-treated rats,motor and memory abilities were reduced,surface expression of sortin andα-synuclein in dopaminergic neurons was reduced,and total sortilin and totalα-synuclein were increased in CPA-treated ***9D cells were incubated with 500 nM CPA alone or in combination with 10μM SP600125(JNK inhibitor)for 48 *** real-time polymerase chain reaction analysis of sortilin andα-synuclein mRNA levels in MN9D cells revealed upregulated sortilin expression in MN9D cells cultured with CPA alone,but the combination of CPA and SP600125 could inhibit this *** made using Jasper,PROMO,and Alibaba online databases identified a highly conserved sequence in the sortilin promoter that was predicted to bind JUN in both humans and rodents.A luciferase reporter assay of sortilin promoter plasmid-transfected HEK293T cells confirmed this *** sortilin expression was inhibited by sh-SORT1,expression of p-JUN and c-JUN was detected by western blot ***-term adenosine A1 receptor activation levels upregulatedα-synuclein expression at the pos

关键词： cognitive dysfunction dopaminergic neuron dyskinesia JNK/c-JUN pathway long-term adenosine A1 receptor activation neural regeneration neurodegenerative diseases sortilin α-synuclein

The role of hypoxia-inducible factors in tumor angiogenesis and cell metabolism

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Genes & Diseases 2017年第1期4卷 19-24页

作者： Xiu lv Jincheng Li Chuhong Zhang Tian Hu Sai Li sha He Hanxing Yan Yixi Tan Mingsheng Lei Meiling Wen Jianhong Zuo The Affiliated Nanhua Hospital University of South ChinaHengyangHunan 421001China School of Medicine University of South ChinaHengyangHunan 421001China Medical School Shaoyang UniversityShaoyangHunan 422000China Department of Respiratory and Critical Care Medicine Zhangjiajie City HospitalZhangjiajieHunan 427000China

Hypoxia-inducible factor(HIF)is a main heterodimeric transcription factor that regulates the cellular adaptive response to hypoxia by stimulating the transcription of a series of hypoxia-inducible *** is frequently upregulated in solid tumors,and the overexpression of HIF can promote tumor progression or aggressiveness by blood vessel architecture and altering cellular *** this review,we focused on the pivotal role of HIF in tumor angiogenesis and energy ***,we also emphasized the possibility of HIF pathway as a potential therapeutic target in cancer.

关键词： Angiogenesis HIF Hypoxia Metabolism VHL

The potential value of microRNA-4463 in the prognosis evaluation in hepatocellular carcinoma

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Genes & Diseases 2017年第2期4卷 116-122页

作者： Tian Hu Jincheng Li Chuhong Zhang Xiu lv Sai Li sha He Hanxing Yan Yixi Tan Mingsheng Lei Meiling Wen Jianhong Zuo The Affiliated Nanhua Hospital University of South ChinaHengyangHunan421001China School of Medicine University of South ChinaHengyangHunan421001China Medical School Shaoyang UniversityShaoyangHunan422000China Department of Respiratory and Critical Care Medicine Zhangjiajie City HospitalZhangjiajieHunan427000China

The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma(HCC),and to clarify the meaning of them in the prognosis evaluation in ***-five samples from healthy people and patients,who had been diagnosed with hepatocellular carcinoma before any treatment,were collected to study ***-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma *** expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group(P0.05).But there was a significant difference of different level of AFP in HCC(P<0.05),and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant(P<0.05).Besides,the survival time had showed a significant difference at the high and low expression levels(P<0.05).But the expression level of miRNA-6087 was no difference in HCC and control *** disorder of miRNA-4463 occurred in HCC,even the AFP level doesn’t ***’s more,patients who get the high level of miRNA-4463 seem to have a shorter survival *** it contributes great to the prognostic *** is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC.

关键词： Hepatocellular carcinoma malignant tumors microRNA-4463 microRNA-6087 miR-4463 Prognostic

奶牛乳腺炎中凝固酶阴性葡萄球菌的分离鉴定及耐药性分析

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中国奶牛 2024年第7期 51-55页

作者：岳丽莎武岳吕雅琼何一平李嵩樊俊铭内蒙古优然牧业有限责任公司呼和浩特010080

为减少兽用抗菌药的使用,建立适用于牧场特色的凝固酶阴性葡萄球菌(CNS)乳腺炎奶牛的精准用药方案,对采集自内蒙古呼和浩特市某规模化牛场的262份乳腺炎乳样,采用鉴别显色培养基分离、传统培养和16S rRNA核酸序列鉴定等,对乳腺炎致病菌... 详细信息

为减少兽用抗菌药的使用,建立适用于牧场特色的凝固酶阴性葡萄球菌(CNS)乳腺炎奶牛的精准用药方案,对采集自内蒙古呼和浩特市某规模化牛场的262份乳腺炎乳样,采用鉴别显色培养基分离、传统培养和16S rRNA核酸序列鉴定等,对乳腺炎致病菌菌种进行分析;后续对分离得到的53株CNS,进行了6种常用药物的MIC试验(微量肉汤稀释法)并分析其耐药性。结果表明,262份乳腺炎样品分离鉴定的致病菌种类丰富,共有20个属,54个种,葡萄球菌属是262个分离株的优势菌属,也是鉴别显色培养基上葡萄球菌区的优势菌属;分析表明CNS可能是长期导致牛场奶牛乳腺炎的主要致病菌。药敏试验结果显示,53株CNS在6种抗菌药物中,对头孢氨苄和马波沙星的耐药率较高,分别为47.17%和49.06%,对头孢噻呋的耐药率最低,为16.98%;在四种β-内酰胺类药物中,对头孢噻呋的敏感性是最高的,达到83.02%,头孢噻呋较头孢喹肟的CNS抗菌活性更好。本次研究分离得到的CNS中,分离株最多的是产色葡萄球菌,对青霉素和头孢噻呋的耐药率较低;其次是模仿葡萄球菌,对头孢噻呋的耐药率最低;溶血葡萄球菌对头孢噻呋和头孢喹肟耐药率较低;松鼠葡萄球菌对头孢氨苄、青霉素和头孢噻呋的耐药率较低。表明不同的CNS菌种对不同的抗生素的敏感性相差较大。本研究为该地区奶牛乳腺炎的管理和防治提供了科学依据。

关键词：奶牛乳腺炎凝固酶阴性葡萄球菌分离鉴定耐药性 16S rDNA

Manganese salts function as potent adjuvants

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Cellular & Molecular Immunology 2021年第5期18卷 1222-1234页

作者： Rui Zhang Chenguang Wang Yukun Guan Xiaoming Wei Mengyin sha Mengran Yi Miao Jing Mengze lv Wen Guo Jing Xu Yi Wan Xin-Ming Jia Zhengfan Jiang Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education School of Life SciencesPeking UniversityBeijingChina Peking-Tsinghua Center for Life Sciences Peking UniversityBeijingChina Clinical Medicine Scientific and Technical Innovation Center Shanghai Tenth People’s HospitalTongji University School of MedicineShanghaiChina CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety National Center for Nanoscience and Technology of ChinaBeijingChina Laboratory for Earth Surface Processes College of Urban and Environmental SciencesPeking UniversityBeijingChina

Aluminum-containing adjuvants have been used for nearly 100 years to enhance immune responses in billions of doses of *** date,only a few adjuvants have been approved for use in humans,among which aluminum-containing adjuvants are the only ones widely ***,the medical need for potent and safe adjuvants is currently continuously increasing,especially those triggering cellular immune responses for cytotoxic T lymphocyte activation,which are urgently needed for the development of efficient virus and cancer *** is an essential micronutrient required for diverse biological activities,but its functions in immunity remain *** previously reported that Mn^(2+) is important in the host defense against cytosolic dsDNA by facilitating cGAS-STING activation and that Mn^(2+)alone directly activates cGAS independent of dsDNA,leading to an unconventional catalytic synthesis of 2′3′-***,we found that Mn^(2+) strongly promoted immune responses by facilitating antigen uptake,presentation,and germinal center formation via both cGAS-STING and NLRP3 ***,a colloidal manganese salt(Mn jelly,MnJ)was formulated to act not only as an immune potentiator but also as a delivery system to stimulate humoral and cellular immune responses,inducing antibody production and CD4^(+)/CD8^(+)T-cell proliferation and activation by either intramuscular or intranasal *** administered intranasally,MnJ also worked as a mucosal adjuvant,inducing high levels of secretory *** showed good adjuvant effects for all tested antigens,including T cell-dependent and T cell-independent antigens,such as bacterial capsular polysaccharides,thus indicating that it is a promising adjuvant candidate.

关键词： Manganese(Mn^(2+)) adjuvant cGAS-STING NLRP3 antigen presentation

Application of Wavelet Transform Modulus Maxima in Raman Distributed Temperature Sensors

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Photonic Sensors 2014年第2期4卷 142-146页

作者： Zongliang WANG Jun CHANG Sasa ZHANG sha LUO Cuanwu JIA Boning SUN Shuo JIANG Yongning LIU Xiaohui LIU Guangping lv Zhi LIU School of Information Science and Engineering and shandong Provincial Key Laboratory of Laser Technology andApplieation Shandong University Jinan 250100 China

We proposed and demonstrated a wavelet transform modulus maxima （WTMM） de-noising method to decrease the temperature error. In this scheme, the composition scale was determined simply by the WTMM amplitude variation with the growth of the decomposition scale at 30 ℃, and the signal WTMM was obtained by the wavelet decomposition modulus on every decomposition scale based on the modulus propagating difference between the signal and noise. Then, we reconstructed the signal using the signal WTMM. Experimental results show that the proposed method is effective for de-noising, allowing for a temperature error decrease of about 1 ℃ at 40 ℃ and 50℃ comparing to the original data.

关键词： Distributed temperature sensor Raman wavelet transform modulus maxima

基于心脏磁共振晚期钆增强的心肌信号强度异质性对心肌炎患者预后价值的研究

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磁共振成像 2024年第3期15卷 68-73页

作者：刘方圆高艳王文先史荣超李莎王锡明山东大学医学融合与实践中心济南250012 山东省立医院影像科济南250021

目的探讨左心室熵在心肌炎患者预后评估中的应用价值。材料与方法收集自2015年7月至2023年10月山东省立医院临床诊断为心肌炎的患者共56例,分析其临床指标、心脏磁共振参数及左心室熵。根据患者是否发生主要不良心血管事件(major adve... 详细信息

目的探讨左心室熵在心肌炎患者预后评估中的应用价值。材料与方法收集自2015年7月至2023年10月山东省立医院临床诊断为心肌炎的患者共56例,分析其临床指标、心脏磁共振参数及左心室熵。根据患者是否发生主要不良心血管事件(major adverse cardiovascular events,MACE)分为预后良好组(42例)和预后不良组(14例);通过受试者工作特征(receiver operator characteristic,ROC)曲线分析评估左心室熵的预测价值,采用生存函数分析(Kaplan-Meier法,K-M)绘制生存曲线,应用Log-rank检验K-M曲线。结果在随访过程中,14例患者(25.00%)发生MACE,预后不良组患者的左心室熵显著增高(P<0.001)。ROC曲线分析显示,左心室熵的ROC曲线下面积为0.871,敏感度为78.5%,特异度为83.2%。K-M分析显示,左心室熵≥7.805患者的总生存率低于左心室熵<7.805患者(P<0.001)。结论左心室熵对预测心肌炎患者MACE的发生有一定的参考价值,有助于临床更好地评价心肌炎患者的预后。

关键词：心肌炎主要心血管不良事件心脏磁共振左心室熵 T1 mapping 磁共振成像

Study on the Seismic Fortification Criteria of Offshore Platforms in the Bohai Sea

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Earthquake Research in China 2013年第1期27卷 109-120页

作者： lv Yuejun Peng Yanju Wang Junqin sha Haijun Institute of Crustal Dynamics China Earthquake Administration CNOOC Research Institute

This paper analyzes the seismicity and seismic risk distribution in the Bohai Sea. Based on the seismic design parameters of 46 platforms in the Bohai Sea, a statistic analysis is made on the ratios of the peak accelerations for different probability levels. In accordance with the two-stage design method, a scheme of two design seismic levels is proposed, and two fortification goals are established respectively for strength level earthquakes and ductility level earthquakes. Through analogy and comparison to the Chinese seismic code for buildings, it is proposed that the probability level for the strength and ductility level earthquakes takes return periods of 200a and 1000～2500a respectively, and we further expounded on its rationality. Finally, the fortification parameters in the sub-regions of Bohai Sea area are given in the light of seismic risk zonation and ground motion division. This article is a summary of experiences from many years of offshore platform seismic fortification work, and an exploratory study on the seismic fortification standards of offshore platforms in China, which may provide some references for the establishment of the standard.

关键词： Bohai Sea Offshore platform Seismic fortification level

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选煤厂重介质分选工艺智能化改造及应用

煤炭加工与综合利用 2024年第3期 10-13,17页

作者：刘新辉袁雪吕鹏辉雷伟刚薛振磊卜祥宁沙杰陕西新能选煤技术有限公司陕西西安710100 中国矿业大学煤炭加工与高效洁净利用教育部重点实验室江苏徐州221116 中国矿业大学化工学院江苏徐州221116 山西长治王庄煤业有限责任公司山西长治047100

针对陕西某选煤厂入选煤质变化较大、集成化不高、智能化程度底等问题,结合煤炭行业智能化应用与发展趋势,通过对选煤工艺流程和洗选装备进行分析,以自动化为基础,准确拾取底层数据,建立生产模型,主要针对重介系统、浓缩系统等环节进行... 详细信息

针对陕西某选煤厂入选煤质变化较大、集成化不高、智能化程度底等问题,结合煤炭行业智能化应用与发展趋势,通过对选煤工艺流程和洗选装备进行分析,以自动化为基础,准确拾取底层数据,建立生产模型,主要针对重介系统、浓缩系统等环节进行改造,提高了选煤厂生产的科学性和稳定性,实现了降本增效、安全生产。

关键词：选煤厂智能化工艺流程改造应用