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Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons

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Nanotechnology and Precision Engineering 2024年第2期7卷 1-10页

作者： Yan Yang Shihong Xu Yu Deng Yaoyao Liu Kui Zhang Shiya lv Longze sha Qi Xu Xinxia Cai Jinping Luo State Key Laboratory of Transducer Technology Aerospace Information Research InstituteChinese Academy of SciencesBeijing 100190China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Medical Molecular Biology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100005China

To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip was designed based on the specific structure of neurons and the requirements for detection and modulation.Finite-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic channels.By employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated successfully.Primary hippocampal neurons were cultured on the chip,forming a well-defined neural network.Spontaneous electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode array.This study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research.

关键词： Microﬂuidic Microelectrode array In vitro Neural chip

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Analyzing the surface passivity effect of germanium oxynitride:a comprehensive approach through first principles simulation and interface state density

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Nuclear Science and Techniques 2024年第5期35卷 74-84页

作者： Sheng-Jie Du Xiu-Xia Li Yang Tian Yuan-Yuan Liu Ke Jia Zhong-Zheng Tang Jian-Ping Cheng Zhi Deng Yu-Lan Li Zheng-Cao Li sha-sha lv College of Nuclear Science and Technology Joint Laboratory of Jinping Ultra‑Low Radiation Background Measurement of Ministry of Ecology and Environment Key Laboratory of Beam Technology of Ministry of Education Beijing Normal UniversityBeijing Normal UniversityBeijing 100875China Joint Research Center Nuctech Company LimitedBeijing 100084China Department of Engineering Physics Key Laboratory of Particle&Radiation Imaging of Ministry of EducationTsinghua UniversityBeijing 100084China Key Laboratory of Advanced Materials(MOE) School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China

High-purity germanium(HPGe)detectors,which are used for direct dark matter detection,have the advantages of a low threshold and excellent energy resolution.The surface passivation of HPGe has become crucial for achieving an extremely low energy threshold.In this study,first-principles simulations,passivation film preparation,and metal oxide semiconductor(MOS)capacitor characterization were combined to study surface passivation.Theoretical calculations of the energy band structure of the -H,-OH,and -NH_(2) passivation groups on the surface of Ge were performed,and the interface state density and potential with five different passivation groups with N/O atomic ratios were accurately analyzed to obtain a stable surface state.Based on the theoretical calculation results,the surface passivation layers of the Ge_(2)ON_(2) film were prepared via magnetron sputtering in accordance with the optimum atomic ratio structure.The microstructure,C-V,and I-V electrical properties of the layers,and the passivation effect of the Al/Ge_(2)ON_(2)/Ge MOS were characterized to test the interface state density.The mean interface state density obtained by the Terman method was 8.4×10^(11) cm^(-2) eV^(-1).The processing of germanium oxynitrogen passivation films is expected to be used in direct dark matter detection of the HPGe detector surface passivation technology to reduce the detector leakage currents.

关键词： Surface passivation High purity germanium detector Germanium nitrogen oxide Interface state density

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Analysis of Etiology,Diagnosis,and Treatment Strategy and Efficacy of Pulsatile Tinnitus Caused by Abnormal Vascular Anatomy

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Current Medical Science 2023年第1期43卷 173-183页

作者： sha lv Jia-tian YUAN Qi-wei XIE Shi-xiong TANG Yao-wen WANG Department of Otolaryngology Ningbo First HospitalNingbo315000China

Objective To investigate the etiology,clinical characteristics,diagnosis,and treatment strategies and efficacy of pulsatile tinnitus(PT)caused by vascular anatomy abnormality.Methods The clinical data of 45 patients with PT in our hospital from 2012 to 2019 were collected and retrospectively analyzed.Results All 45 patients had vascular anatomical abnormalities.The patients were divided into 10 categories according to the different locations of vascular abnormalities:sigmoid sinus diverticulum(SSD),sigmoid sinus wall dehiscence(SSWD),SSWD with high jugular bulb,pure dilated mastoid emissary vein,aberrant internal carotid artery(ICA)in the middle ear,transverse-sigmoid sinus(TSS)transition stenosis,TSS transition stenosis with SSD,persistent occipital sinus stenosis,petrous segment stenosis of ICA,and dural arteriovenous fistula.All patients complained of PT synchronous with heartbeat rhythm.Endovascular interventional therapy and extravascular open surgery were used according to the location of the vascular lesions.Tinnitus disappeared in 41 patients,was significantly relieved in 3 patients,and was unchanged in 1 patient postoperatively.Except for one patient with transient headache postoperatively,no obvious complications occurred.Conclusion PT caused by vascular anatomy abnormalities can be identified by detailed medical history and physical and imaging examination.PT can be relieved or even completely alleviated after appropriate surgical treatments.

关键词： pulsatile tinnitus vessel structural abnormity etiology treatment strategy efficacy

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Gli1 promotes epithelial-mesenchymal transition and metastasis of non-small cell lung carcinoma by regulating snail transcriptional activity and stability

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Acta Pharmaceutica Sinica B 2022年第10期12卷 3877-3890页

作者： Xueping Lei Zhan Li Yihang Zhong Songpei Li Jiacong Chen Yuanyu Ke sha lv Lijuan Huang Qianrong Pan Lixin Zhao Xiangyu Yang Zisheng Chen Qiudi Deng Xiyong Yu Guangzhou Municipal and Guangdong Provincial Key Laboratory of Molecular Target&Clinical Pharmacology the NMPA and State Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences and the Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China Department of Respiratory and Critical Care Medicine the Sixth Affiliated Hospital of Guangzhou Medical UniversityQingyuan People’s HospitalQingyuan 511518China GMU-GIBH Joint School of Life Sciences Guangzhou Medical UniversityGuangzhou 511436China

Metastasis is crucial for the mortality of non-small cell lung carcinoma(NSCLC) patients.The epithelial-mesenchymal transition(EMT) plays a critical role in regulating tumor metastasis.Glioma-associated oncogene 1(Gli1) is aberrantly active in a series of tumor tissues. However, the molecular regulatory relationships between Gli1 and NSCLC metastasis have not yet been identified. Herein,we reported Gli1 promoted NSCLC metastasis. High Gli1 expression was associated with poor survival of NSCLC patients. Ectopic expression of Gli1 in low metastatic A549 and NCI-H460 cells enhanced their migration, invasion abilities and facilitated EMT process, whereas knock-down of Gli1 in high metastatic NCI-H1299 and NCI-H1703 cells showed an opposite effect. Notably, Gli1 overexpression accelerated the lung and liver metastasis of NSCLC in the intravenously injected metastasis model. Further research showed that Gli1 positively regulated Snail expression by binding to its promoter and enhancing its protein stability, thereby facilitating the migration, invasion and EMT of NSCLC. In addition, administration of GANT-61, a Gli1 inhibitor, obviously suppressed the metastasis of NSCLC. Collectively, our study reveals that Gli1 is a critical regulator for NSCLC metastasis and suggests that targeting Gli1 is a prospective therapy strategy for metastatic NSCLC.

关键词： Non-small cell lung carcinoma Metastasis Epithelialemesenchymal transition Glioma-associated oncogene 1 Promote Snail Protein stability GANT-61

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Numerical study of liquid coverage in a gas-liquid-solid packed bed

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Particuology 2015年第6期13卷 90-99页

作者： Wei Du Lifeng Zhang sha lv Panpan Lu Jian xu Weisheng Wei State Key Laboratory of Heavy Oil Processing China University of Petroleum Beijing 102249 China The Key Laboratory of Catalysis China National Petroleum Corp. China University of Petroleum Beijing 102249 China Department of Chemical and Biological Engineering University of Saskatchewan Saskatoon SK Canada

In this work, computational fluid dynamics （CFD） simulations using the volume-of-fluid （VOF） model were employed to investigate the effects of liquid properties, liquid and gas flow rates, and wettability of particles on liquid maldistribution at the microscopic level in a fixed bed reactor. The simulation results show that the number of wetted particles decreases with increasing gas velocity, consequently leading to lower liquid-solid contact areas. The radial liquid distribution is greatly enhanced by increasing the liquid flow rate, whereas the time for the liquid to pass through the whole bed is decreased, as expected. Based on simulation results, it was found that the liquid-solid contact area can be increased by using liquids of high viscosities and more wettable particles. However, the flow-through time increases with increasing liquid viscosity. An increase in the gas density showed a minimal impact on the liquid flow- through time, and the liquid density does not impact the radial liquid distribution or the liquid flow time within a range of liquid densities typically encountered in the petrochemical industry.

关键词： CFD VOF Liquid-solid contact area Radial liquid distribution Fixed bed

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预输血患者直接抗人球蛋白试验结果分析

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医学动物防制 2024年第2期40卷 206-208,F0003页

作者：王红于惠敏徐伟乐吴小静吕翠环吕莎河北省胸科医院输血科河北石家庄050041 河北省胸科医院普通外科河北省胸科医院呼吸与重症医学一科

目的探讨直接抗人球蛋白试验(direct antiglobulin test, DAT)阳性在预输血患者中的分布情况。方法回顾性分析2020年6月-2021年7月河北省某医院住院的1 587例预输血患者的DAT结果,运用SPSS 19.0软件统计分析患者的年龄、性别、科室、... 详细信息

目的探讨直接抗人球蛋白试验(direct antiglobulin test, DAT)阳性在预输血患者中的分布情况。方法回顾性分析2020年6月-2021年7月河北省某医院住院的1 587例预输血患者的DAT结果,运用SPSS 19.0软件统计分析患者的年龄、性别、科室、ABO血型与DAT阳性的关系。结果预输血患者的DAT阳性率为13.23%(210/1 587),随年龄增大,患者的DAT阳性率呈上升趋势,50~70岁年龄组患者的DAT阳性率最高(χ^(2)=7.496,P=0.006);女性患者的DAT阳性率高于男性患者(P’_男=13.305,P’_女=20.460);不同科室间患者的DAT阳性率差异有统计学意义(χ^(2)=242.931,P<0.001),重症监护室、其他内科、肾内科、消化内科和呼吸科最高(χ^(2)=14.170,P<0.001),急诊科、肿瘤内科和泌尿外科次之(χ^(2)=18.812,P<0.001)。不同ABO血型患者的DAT阳性率差异有统计学意义(χ^(2)=7.981,P=0.046),B型血患者的DAT阳性率最低(χ^(2)=5.472,P=0.020)。结论 DAT阳性率与预输血患者的年龄、性别、科室和ABO血型均存在相关性。

关键词：直接抗人球蛋白试验年龄性别科室 ABO血型

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Insights into varicella-zoster virus assembly from the B-and C-capsid at near-atomic resolution structures

hLife

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hLife 2024年第2期2卷 64-74页

作者： Lei Cao Nan Wang Zhe lv Wenyuan Chen Zhonghao Chen Lifei Song Xueyan sha Guiqiang Wang Yaling Hu Xiaojun Lian Guoliang Cui Jinyan Fan Yaru Quan Hongrong Liu Hai Hou Xiangxi Wang CAS Key Laboratory of Infection and Immunity National Laboratory of MacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijingChina Key Laboratory for Matter Microstructure and Function of Hunan Province Key Laboratory of Low-dimensional Quantum Structures and Quantum ControlSchool of Physics and ElectronicsHunan Normal UniversityHunanChina Institute of Medical Research Northwestern Polytechnical UniversityShanxiChina Sinovac(Dalian)Vaccine Technology Co. Ltd.LiaoningChina Sinovac Biotech Ltd. BeijingChina National Institutes for Food and Drug Control BeijingChina

Varicella-zoster is a highly communicable virus that can be transmitted through the airborne route.About one quarter of people are infected with this virus.Previous studies have described the structure of A-capsid and a blurred reconstruction of the C-capsid with icosahedral symmetry.In this study,we have determined the more precise detailed structures of the varicella-zoster virus(VZV)B-and C-capsid in icosahedral symmetry using a combination of block-based reconstruction and symmetry relaxation strategies.In addition,we are reporting structural details of the portal vertex reconstructions in five-fold symmetry and portal reconstructions in twelve-fold symmetry.The structures unveil the basis for the high thermal stability of the VZV capsid.The conformational flexibility of structural elements of the capsid plays a role in the assembly of the capsid and drives processes critical for the viral life cycle.The results of the study open up new avenues for the development of drugs against a highly prevalent and contagious pathogen.

关键词： varicella-zoster virus capsid structure virus assembly

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Itraconazole therapy for infant hemangioma:Two case reports

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World Journal of Clinical Cases 2021年第28期9卷 8579-8586页

作者： Zhe Liu sha lv Shuang Wang Sheng-Ming Qu Gui-Yun Zhang Yi-Tong Lin Lei Yang Fu-Qiu Li Department of Dermatology The Second Hospital of Jilin UniversityChangchun 130041Jilin ProvinceChina

BACKGROUND Infantile hemangiomas(IHs)are the most common childhood benign tumors,showing distinctive progression characteristics and outcomes.Due to the high demand for aesthetics among parents of IH babies,early intervention is critical in some cases.β-Adrenergic blockers and corticosteroids are first-line medications for IHs,while itraconazole,an antifungal medicine,has shown positive results in recent years.CASE SUMMARY In the present study,itraconazole was applied to treat two IH cases.The therapeutic course lasted 80-90 d,during which the visible lesion faded by more than 90%.Moreover,no obvious side effects were reported,and the compliance of the baby and parents was desirable.CONCLUSION Although these outcomes further support itraconazole as an effective therapeutic choice for IHs,large-scale clinical and basic studies are still warranted to improve further treatment.

关键词： Infant Hemangiomas Therapeutics Itraconazole Oral Case report

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Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes

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Science China Life Sciences 2024年第8期 1697-1714页

作者： Huan Wu Yanman Zhang Yuqian Li Shuya Sun Jintao Zhang Qingsong Xie Yue Dong Shushu Zhou Xuan sha Kuokuo Li Jinyi Chen Xin Zhang Yang Gao Qunshan Shen Guanxiong Wang Xiaomin Zha Zongliu Duan Dongdong Tang Chuan Xu Hao Geng Mingrong lv Yuping Xu Ping Zhou Zhaolian Wei Rong Hua Yunxia Cao Mingxi Liu Xiaojin He Reproductive Medicine Center Department of Obstetrics and GynecologyThe First Affiliated Hospital of Anhui Medical University NHC Key Laboratory of Study on Abnormal Gametes and Reproductive Tract (Anhui Medical University) Key Laboratory of Population Health Across Life Cycle (Anhui Medical University) Ministry of Education of the People's Republic of China State Key Laboratory of Reproductive Medicine and Offspring Health Department of Histology and EmbryologySchool of Basic Medical SciencesNanjing Medical University State Key Laboratory of Reproductive Medicine and Offspring Health Clinical Center of Reproductive MedicineThe First Affiliated Hospital of Nanjing Medical University Anhui Province Key Laboratory of Reproductive Health and Genetics Biopreservation and Artificial Organs Anhui Provincial Engineering Research CenterAnhui Medical University State Key Laboratory of Reproductive Medicine and Offspring Health The Affiliated Taizhou People's Hospital of Nanjing Medical UniversityTaizhou School of Clinical MedicineNanjing Medical University Reproductive Medicine Center Department of Obstetrics and GynecologyShanghai General HospitalShanghai Jiao Tong University School of Medicine

The complexities of energy transfer mechanisms in the flagella of mammalian sperm flagella have been intensively investigated and demonstrate significant diversity across species.Enzymatic shuttles,particularly adenylate kinase(AK) and creatine kinase(CK),are pivotal in the efficient transfer of intracellular ATP,showing distinct tissue-and species-specificity.Here,the expression profiles of AK and CK were investigated in mice and found to fall into four subgroups,of which Subgroup Ⅲ AKs were observed to be unique to the male reproductive system and conserved across chordates.Both AK8 and AK9 were found to be indispensable to male reproduction after analysis of an infertile male cohort.Knockout mouse models showed that AK8 and AK9 were central to promoting sperm motility.Immunoprecipitation combined with mass spectrometry revealed that AK8 and AK9 interact with the radial spoke(RS) of the axoneme.Examination of various human and mouse sperm samples with substructural damage,including the presence of multiple RS subunits,showed that the head of radial spoke 3acts as an adapter for AK9 in the flagellar axoneme.Using an ATP probe together with metabolomic analysis,it was found that AK8 and AK9cooperatively regulated ATP transfer in the axoneme,and were concentrated at sites associated with energy consumption in the flagellum.These findings indicate a novel function for RS beyond its structural role,namely,the regulation of ATP transfer.In conclusion,the results expand the functional spectrum of AK proteins and suggest a fresh model regarding ATP transfer within mammalian flagella.

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Static and dynamic evolution of CO adsorption onγ-U(100)surface with different levels of Mo doping using DFT and AIMD calculations

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Nuclear Science and Techniques 2023年第9期34卷 142-154页

作者： Jun-Wei Li Wei-Min Jia Chong Liu sha-sha lv Jin-Tao Wang Zheng-Cao Li Key Lab of Advanced Materials(MOE) School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Xi’an Research Institute of High-Technology Xi’an 710025China Key Laboratory of Beam Technology(MOE) College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China

Alloys of uranium and molybdenum are considered as the future of nuclear fuel and defense materials.However,surface corrosion is a fundamental problem in practical applications and storage.In this study,the static and dynamic evolution of carbon monoxide(CO)adsorption and dissociation onγ-U(100)surface with different Mo doping levels was investigated based on density functional theory and ab initio molecular dynamics.During the static calculation phase,parameters,such as adsorption energy,configuration,and Bader charge,were evaluated at all adsorption sites.Furthermore,the time-dependent behavior of CO molecule adsorption were investigated at the most favorable sites.The minimum energy paths for CO molecu-lar dissociation and atom migration were investigated using the transition state search method.The results demonstrated that the CO on the uranium surface mainly manifests as chemical adsorption before dissociation of the CO molecule.The CO molecule exhibited a tendency to rotate and tilt upright adsorption.However,it is difficult for CO adsorption on the surface in one of the configurations with CO molecule in vertical direction but oxygen(O)is closer to the surface.Bader charge illustrates that the charge transfers from slab atoms to the 2π*antibonding orbital of CO molecule and particularly occurs in carbon(C)atoms.The time is less than 100 fs for the adsorptions that forms embryos with tilt upright in dynamics evolution.The density of states elucidates that the overlapping hybridization of C and O 2p orbitals is mainly formed via the d orbitals of uranium and molybdenum(Mo)atoms in the dissociation and re-adsorption of CO molecule.In conclusion,Mo doping of the surface can decelerate the adsorption and dissociation of CO molecules.A Mo-doped surface,created through ion injection,enhanced the resistance to uranium-induced surface corrosion.

关键词： Adsorption and dissociation Uranium CO molecule Density functional theory Ab initio molecular dynamics

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