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Drag reduction and hairpin packets of the turbulent boundary layer over the superhydrophobic-riblets surface

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Journal of Hydrodynamics 2021年第3期33卷 621-635页

作者： Xin-wei Wang Zi-ye Fan Zhan-qi Tang Nan Jiang School of Mechanical Engineering Tianjin UniversityTianjin300354China Tianjin Key Laboratory of Modern Engineering Mechanics Tianjin300354China

This paper presents experimental measurements by the time-resolved particle image velocimetry(TR-PIV)for the turbulent boundary layer(TBL)over the smooth surface,the superhydrophobic(sh)surface,and the superhydrophobic-riblets(SR)surface in an open-surface recirculating water *** Reynolds number based on the wall friction velocity and the thickness of the TBL over the smooth surface is *** sh surface and the SR surface are manufactured by the laser texturing method on the smooth surface and the riblets surface,*** employing the(modified)Clauser method,a superior efficacy of the drag reduction of about 22.1%is obtained on the SR surface,while the drag reduction rate for the sh surface is about 18.7%.Comparing with the sh surface,the declining 2-order statistics in the near-wall region also indicates a significant drag reduction over the SR *** large-scale structure components extracted by the proper orthogonal decomposition are found to generate a majority of the Reynolds shear stress in the region y^(+)>*** strength of the large-scale features over the rough surfaces(sh and SR surfaces)at disparate wall-normal positions is visualized by the Quadrant splitting method and the conditional *** appearance of the large-scale structures such as the hairpin packets characterized by the two-point correlation shows an excellent consistency with the statistics *** hairpin packets over the sh surface seem always smaller and weaker than those over the smooth *** the SR surface,the hairpin packets in the region 0.1≤y/δ<0.3 are the smallest and weakest among those over the three surfaces,while the scale and the strength of the hairpin packets exceed those over the smooth surface in the region 0.3

关键词： Turbulent boundary layer superhydrophobic riblets drag reduction hairpin packets

CD36与肝细胞癌细胞增殖和迁移的关系及其对人肝癌细胞异种移植裸鼠模型的影响

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肿瘤研究与临床 2024年第2期36卷 98-104页

作者：张立洪吴巍胥广才张培建陶立德扬州大学附属医院肝胆外科扬州225012

目的观察CD36在肝细胞癌组织和细胞株中的表达水平,探讨CD36对人肝细胞癌细胞株增殖、迁移能力及人肝癌细胞异种移植裸鼠模型的影响。方法基于癌症基因组图谱(TCGA)数据库相关信息分析371份肝细胞癌及癌旁组织中CD36转录本表达水平差异... 详细信息

目的观察CD36在肝细胞癌组织和细胞株中的表达水平,探讨CD36对人肝细胞癌细胞株增殖、迁移能力及人肝癌细胞异种移植裸鼠模型的影响。方法基于癌症基因组图谱(TCGA)数据库相关信息分析371份肝细胞癌及癌旁组织中CD36转录本表达水平差异。前瞻性收集2019年1月至2021年2月就诊于扬州大学附属医院行手术治疗的48例肝细胞癌患者癌组织及相应的癌旁组织,采用实时荧光定量聚合酶链反应(qRT-PCR)法检测组织中CD36 mRNA水平。采用蛋白质印迹法检测人肝癌细胞株Huh7、HCCLM3及人正常肝细胞株LO2中CD36蛋白水平。将载有CD36干扰序列的质粒和空质粒转染到Huh7细胞或HCCLM3细胞,分别为sh-CD36组和对照组;采用CCK-8法检测培养0、12、24、36、48、60 h各组细胞的增殖能力(以吸光度值表示),采用划痕愈合实验、Transwell实验检测各组细胞迁移能力。将sh-CD36组或对照组Huh7细胞注射于BALB/c裸鼠腋窝皮下,每组4只,构建人肝癌异种移植裸鼠模型;接种1周后每周测量肿瘤长径、短径并计算肿瘤体积,接种5周后处死裸鼠,收集肿瘤标本并称量质量;显微镜下观察肿瘤组织细胞形态,采用免疫组织化学法检测肿瘤组织中CD36、Ki-67蛋白表达情况。结果对TCGA数据库数据分析显示,肝癌组织中CD36转录本水平较癌旁组织高(4.2±1.8比3.2±1.5,t=2.28,P=0.035)。qRT-PCR法对48例肝细胞癌患者组织检测显示,肝癌组织中CD36 mRNA相对表达量高于癌旁组织(0.76±0.26比0.48±0.23,t=3.52,Psh-CD36组Huh7和HCCLM3细胞CD36均低于对应的对照组(均Psh-CD36组Huh7细胞和HCCLM3细胞分别于培养36 h和24 h开始增殖能力均低于对应的对照组(均Psh-CD36组Huh7细胞[(12±3)%比(30±5)%,t=4.01,Psh-CD36组Huh7细胞[(46±6)个/视野比(88±6)个/视野,t=5.56,Psh-CD36组Huh7细胞的裸鼠肿瘤体积[(682±268)mm3比(1375±512)mm3,t=4.73,P=0.006]和肿瘤质量[(432±95)mg/只比(871±109)mg/只,t=6.57,Psh-CD36组Huh7细胞的裸鼠移植瘤标本中肿瘤细胞密度低于注射对照组Huh7细胞的裸鼠,CD36和Ki-67蛋白的表达水平亦均低。结论CD36在肝细胞癌患者癌组织及人肝癌Huh7、HCCLM3细胞株中表达均上调,其可能与肝癌细胞增殖和迁移相关。敲低CD36表达体外可明显抑制肝癌细胞增殖和迁移能力,对人肝癌细胞异种移植裸鼠模型肿瘤有抑制作用。

关键词：癌,肝细胞抗原,CD36 细胞增殖细胞运动

基于GEO数据库前列腺癌促癌基因NUDT11的筛选及验证

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赣南医学院学报 2024年第8期44卷 768-774,779页

作者：吴宇王升通徐卫强蚌埠医科大学第二附属医院蚌埠医科大学安徽蚌埠233000

目的:探讨影响前列腺癌进展的差异表达基因,为其诊治提供新思路。方法:⑴采用生物信息学和SVM-RFE机器学习方法筛选出影响前列腺癌进展的差异表达基因(Different Express Genes,DEGs),使用“clusterProfiler”R包对差异表达基因进行基... 详细信息

目的:探讨影响前列腺癌进展的差异表达基因,为其诊治提供新思路。方法:⑴采用生物信息学和SVM-RFE机器学习方法筛选出影响前列腺癌进展的差异表达基因(Different Express Genes,DEGs),使用“clusterProfiler”R包对差异表达基因进行基因本体(Gene Ontology,GO)富集分析和基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析,并筛选关键基因。⑵RT-PCR验证前列腺癌细胞PC3和LNCaP NUDT11 mRNA表达。⑶将前列腺癌细胞LNCaP、PC3分为si-NC组和si-NUDT11组,采用脂质体法进行瞬时转染,转染成功后进行细胞克隆实验、EdU实验和细胞凋亡实验观察NUDT11对细胞增殖及凋亡的影响。⑷构建稳定低表达NUDT11细胞株进行裸鼠皮下成瘤实验,观察NUDT11对前列腺癌细胞成瘤能力的影响。结果:⑴在GEO数据集GSE46602中,共筛选187个DEGs。与正常前列腺组织相比,前列腺癌组织中76个基因表达上调,111个基因表达下调。对差异表达基因进行GO富集分析发现:生物学过程(Biological Process,BP),DEGs主要富集在上皮细胞增殖、上皮细胞增殖的调节、跨膜受体蛋白丝氨酸/苏氨酸、细胞外基质组织、细胞外结构组织、外部封装结构组织、上皮细胞对生长因子刺激反应的调节;细胞学组分(Cytological Components,CC),DEGs主要富集在含胶原的细胞外基质、基底膜、突触囊泡、外囊泡;分子生物学功能(Molecular Biological Functions,MF),DEGs在硫化物结合、肝素结合、细胞外基质结构成分、寡肽结合中富集。对差异表达基因的KEGG富集分析发现DEGs在PI3K-Akt信号转导通路、人乳头瘤病毒感染、癌症中的转录错调、动脉粥样硬化和流体剪切应力、前列腺癌富集。使用SVM-RFE机器学习方法和随机森林(Random forest,RF)特征选择筛选基因,得到共同关键基因为NUDT11和GGTLC1。⑵与前列腺上皮细胞RWPE-1相比,前列腺癌细胞PC3和LNCaP中的NUDT11 mRNA表达量增加。⑶平板克隆实验结果显示与si-NC组相比,si-NUDT11组细胞克隆形成数量均显著下降(Psh-NC组相比,sh-NUDT11组细胞成瘤能力显著下降,瘤体重量变小(P<0.05)。结论:生物信息学和SVM-RFE机器学习方法筛选出与前列腺癌进展相关的基因NUDT11和GGTLC1,NUDT11在前列腺癌细胞系中高表达,干扰NUDT11表达后抑制前列腺癌细胞增殖、促进前列腺癌细胞凋亡,提示NUDT11可调控前列腺癌进展。

关键词：前列腺癌促癌基因 NUDT11 筛选

经皮神经电刺激预防剖宫产术后子宫切口缺损形成的疗效观察

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江苏大学学报（医学版） 2024年第3期34卷 260-265页

作者：陈伟鸿王轶群李卫江苏大学第四附属医院妇科江苏镇江212001

目的:观察经皮神经电刺激(transcutaneous electrical nerve stimulation,TENS)预防剖宫产术后子宫切口缺损(previous cesarean scar defect,PCSD)形成的效果。方法:选择2021年2月至2021年11月于江苏大学第四附属医院产科行剖宫产分娩的初产妇151例,随机分为体尾组(n=51)、宫角+体尾组(n=50)和对照组(n=50);对照组行常规产后护理,体尾组于剖宫产术后6 h采用2个电极片分别贴至宫体的体表投影部位和骶尾部,宫角+体尾组于剖宫产术后6 h采用4个电极片分别贴至双侧宫角体表投影、宫体体表投影和骶尾部,行TENS治疗,20 min/次,1次/d,连续3 d;3组分别于产后6周和6个月行阴道超声检查,比较产后6周和6个月子宫大小、憩室形成数量及憩室大小。结果:3组间产妇产后6周和6个月时子宫长度、宽度及厚度比较差异无统计学意义(P>0.05),但6个月时子宫均明显小于产后6周(P均0.05)。3组PCSD形成者产后6周与6个月憩室宽度、深度及残存肌层厚度比较差异均无统计学意义(P>0.05)。结论:在剖宫产术后6 h行TENS可显著降低PCSD形成,采用2个电极片或4个电极片通路对降低PCSD形成效果无明显差异。

关键词：经皮神经电刺激剖宫产术后子宫切口缺损剖宫产憩室

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Heparanase-1 is downregulated in chemoradiotherapy orbital rhabdomyosarcoma and relates with tumor growth as well as angiogenesis

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International Journal of Ophthalmology(English edition) 2022年第1期15卷 31-39页

作者： wei-Qiang Tang Yan Hei Jing Lin Department of Ophthalmology the Fourth Medical CentreChinese PLA General HospitalBeijing 100048China Department of Ophthalmology the Third Medical CentreChinese PLA General HospitalBeijing 100039China Department of Clinical Laboratory the Fourth Medical CentreChinese PLA General HospitalBeijing 100048China

AIM:To determine the role of heparanase-1(HPSE-1)in orbital rhabdomyosarcoma(RMS),and to investigate the feasibility of HPSE-1 targeted therapy for ***:Immunohistochemistry was performed to analyze HPSE-1 expression in 51 cases of orbital RMS patients(including 28 cases of embryonal RMS and 23 cases of alveolar RMS),among whom there were 27 treated and 24 untreated with preoperative *** vitro,studies were conducted to examine the effect of HPSE-1 silencing on RMS cell proliferation and tube formation of human umbilical vein endothelial cells(HUVECs).RD cells(an RMS cell line)and HUVECs were infected with HPSE-1 sh RNA lentivirus at a multiplicity of infection(MOI)of 10 and 30 ***-time PCR and Western blot were applied to detect the m RNA and protein expression levels of *** viability of treated or control RD cells was evaluated by cell counting kit-8(CCK-8)*** tube formation assay was used to evaluate the effect of HPSE-1 RNAi on the tube formation of ***:Immunohistochemistry showed that the expression rate of HPSE-1 protein was 92.9%in orbital embr yonal RMS and 91.3%in orbital alveolar *** from alveolar orbital RMS did not show relatively stronger staining than that from the embryonal orbital ***,despite the types of RMS,comparing the cases treated chemoradiotherapy with those untreated,we have observed that chemoradiotherapy resulted in weaker staining in patients’*** expression levels of HPSE-1 declined significantly in both the m RNA and protein levels in HPSE-1 sh RNA transfected RD *** CCK-8 assay showed that lentivirus-mediated HPSE-1 silencing resulted in significantly reduced RD cells viability in *** HPSE-1 expression also inhibited VEGF-induced tube formation of HUVECs in ***:HPSE-1 silencing may be a promising therapy for the inhibition of orbital RMS progression.

关键词： heparanase-1 rhabdomyosarcoma CCK-8 tube formation chemoradiotherapy

COVID-19背景下上海大气CO_(2)浓度特征及潜在源分析

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中国环境科学 2024年第9期44卷 4864-4873页

作者：郭朋卿雪梅林溢臧昆鹏陈圆圆刘硕陈炳江兰文港高伟方双喜浙江工业大学环境学院浙江碳中和创新研究院浙江省碳减排与碳监测技术国际科技合作基地浙江杭州310014 上海市气象局上海200030

基于2021年4月~2022年12月上海中心大厦(上海站)的CO_(2)浓度观测记录,研究了上海大气CO_(2)浓度特征和影响因素,重点分析台风“烟花”登陆期、春节期间和COVID-19疫情封控期的CO_(2)浓度差异.结果表明:上海站大气CO_(2)日变化特征均呈... 详细信息

基于2021年4月~2022年12月上海中心大厦(上海站)的CO_(2)浓度观测记录,研究了上海大气CO_(2)浓度特征和影响因素,重点分析台风“烟花”登陆期、春节期间和COVID-19疫情封控期的CO_(2)浓度差异.结果表明:上海站大气CO_(2)日变化特征均呈现双峰型(11:00LT和17:00LT),与当地人为活动高峰时段对应.工作日CO_(2)浓度高于周末,浓度差异主要出现在03:00~05:00LT以及早高峰时刻.在4~9月,大气CO_(2)浓度较低,最低值出现在7月((423.42±0.05)×10^(-6)),最高值出现在12月((445.94±0.27)×10^(-6)).台风“烟花”登陆期间,由于降水和强局地风的影响,大气CO_(2)浓度被稀释.春节和疫情封控期间,人为活动影响的减弱显著降低了大气CO_(2)浓度.地面风分析,后向轨迹聚类和潜在源贡献函数(WPSCF)分析表明,来自海洋方向的气团对上海城区大气CO_(2)浓度有稀释作用,上海站西部的安徽,江苏,湖北和浙江等地区的城市交通及工业等人为排放对上海上空大气CO_(2)有明显影响.

关键词：上海长三角高塔大气CO_(2)观测超大城市

Association between CIsh polymorphisms and spontaneous clearance of hepatitis B virus in hepatitis B extracellular antigen-positive patients during immune active phase

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Chinese Medical Journal 2014年第9期127卷 1691-1695页

作者： Song Guangjun Rao Huiying Feng Bo wei Lai Peking University People's Hospital Peking University Hepatology Institute Beijing Key Laboratory of Hepatitis C and Immunotherapy for Liver Diseases Peking University Beijing 100044 China

Background Some hepatitis B extracellular antigen （HBeAg）-positive chronic hepatitis B （CHB） patients in their immune active phase can clear the virus spontaneously and enter into an inactive hepatitis B virus （HBV） carrier state,indicating a benign *** this study,the association between cytokine-inducibie SRC homology 2 domain protein （C/sh） gene polymorphisms at-292 （rs414171） and the spontaneous clearance of HBV in HBeAg-positive CHB patients in immune the active phase was *** Seventy HBeAg-positive CHB patients in the immune active phase were followed up for 76 weeks without antiviral *** alanine transaminase,aspartate transaminase,HBV DNA,HBeAg and hepatitis B extracellular antibody levels were tested *** week 76,27 patients were classified into group A （HBV DNA level below 2 104 IU/ml and the value of HBeAg declined below 10％ of the baseline at week 76）,and 43 patients were classified into group B （HBV DNA level higher than 2×104 IU/ml or the value of HBeAg did not decline substantially at week 76）.CIsh （rs414171） polymorphisms were also tested using the iPLEX *** The HBV DNA levels at week 12 were significantly greater in group B compared with group A （group A：（6.87±1.40） log10IU/ml; group B：（7.61±1.38） log10IU/ml,P=0.034） and the HBeAg values were greater in group B at week 28 compared with group A （P=0.001）.The differences in HBV DNA and HBeAg values increased between the groups over *** patients in group A and 11 in group B were genotype *** with genotype AT or TT included 11 in group A and 31 in group B （AA *** and TT,odds ratio 4.10 （95％ confidence interval：1.462-11.491）,P=0.006）.Conclusion CIsh gene polymorphisms at-292 （rs414171） are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase,and AA is a favorable genotype for this effect.

关键词： CIsh gene polymorphism HBeAg positive chronic hepatitis B HBeAg seroconversion

The Electric Mechanism of Surface Pretreatments for Dye-Sensitized Solar Cells Based on Internal Equivalent Resistance Analysis

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Chinese Physics Letters 2012年第1期29卷 279-282页

作者： XU wei-wei HU Lin-Hua LUO Xiang-Dong LIU Pei-sheng DAI Song-Yuan Jiangsu Provincial Key Lab of ASIC Design Nantong UniversityNantong 226019 Division of Solar Energy Materials and Engineering Institute of Plasma PhysicsChinese Academy of SciencesHefei 230031

Based on the optimization of dye-sensitized solar cell (DSC) photoelectrodes pretreated with different methods such as electrodeposition,spin-coating and TiCl_(4) pretreatment,theoretical calculations are carried out to interpret the internal electric *** numerical values,including the series resistance R_(s) and the shunt resistance R_(sh) corresponding to the equivalent circuit model,are well evaluated and confirm that the DSC has good performance with a high R_(sh) and a low R_(s) due to good electrical contact and a low charge recombination after the different *** Ⅰ-Ⅴ curves are fifted in the case without series resistance,and account for the role of R_(s) in the output *** is found that when R_(s) tends to the infinitesimal,the short-circuit current Isc,the open-circuit voltage V_(oc) and the fill factor can be improved by almost 0.8-1.4,2.9 and 2.1-6.8%,respectively.

关键词： resistance coating Internal

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双质量飞轮(DMF)的研究综述

噪声与振动控制 2008年第5期28卷 1-5页

作者：李伟史文库吉林大学汽车工程学院

DMF和CTD是目前在汽车动力传动系中运用最广的两种扭振减振器。将DMF与CTD的结构形式和隔振性能进行对比分析,简单回顾DMF的发展历程,着重描述多种典型DMF结构特点及性能,并对影响DMF隔振性能的第一飞轮、第二飞轮转动惯量比,弹性特性... 详细信息

DMF和CTD是目前在汽车动力传动系中运用最广的两种扭振减振器。将DMF与CTD的结构形式和隔振性能进行对比分析,简单回顾DMF的发展历程,着重描述多种典型DMF结构特点及性能,并对影响DMF隔振性能的第一飞轮、第二飞轮转动惯量比,弹性特性和阻尼特性等因素进行分析。

关键词：振动与波扭振减振器双质量飞轮离合器从动盘式扭振减振器振动隔离