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Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin

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Insect Science 2024年第2期31卷 435-447页

作者： Jie Hao Chang liu Ning Zhang Jing li Tong Ni Mingbo Qu Xiang-dong li State Key Laboratory oflntegrated Management of lnsect Pests and Rodents Insttute of ZoologyChinese Academy of SciencesBejingChina University of Chinese Academy of Sciences BejingChina School of Bioengeering Dalian University of TechnologyDalianChina

Locust(Locusta migratoria)has a single striated muscle myosin heavy chain(Mhc)gene,which contains 5 clusters of alternative exclusive exons and 1 differently included penultimate *** alternative exons of Mhc gene encode 4 distinct regions in the myosin motor domain,that is,the N-terminal sh3-like domain,one lip of the nucleotide-binding pocket,the relay,and the ***,we investigated the role of the alternative regions on the motor function of locust muscle *** Sf9-baculovirus protein expression system,we expressed and purified 5 isoforms of the locust muscle myosin heavy meromyosin(HMM),including the major isoform in the thorax dorsal longitudinal flight muscle(FL1)and 4 isoforms expressed in the abdominal intersegmental muscle(AB1 to AB4).Among these 5 HMMs,FL1-HMM displayed the highest level of actin-activated adenosine triphosphatase(ATPase)activity(hereafter referred as ATPase activity).To identify the alternative region(s)responsible for the elevated ATPase activity of FL1-HMM,we produced a number of chimeras of FL1-HMM and *** with the relay of AB4-HMM(encoded by exon-14c)substantially decreased the ATPase activity of FL1-HMM,and conversely,the relay of FL1-HMM(encoded by exon-14a)enhanced the ATPase activity of *** showed that the exon-14a-encoded residues Gly474 and Asn509 are responsible for the elevated ATPase activity of *** results indicate that the alternative relay encoded by exon-14a/c play a key role in regulating the ATPase activity of FL1-HMM and AB4-HMM.

关键词： alternative splicing ATPase Locusta migratoria molecular motor muscle myosin

Palmitic acid reduces the methylation of the FOXO1 promoter to suppress the development of diffuse large B-cell lymphoma via modulating the miR-429/DNMT3A axis

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Chinese Journal of Natural Medicines 2024年第6期22卷 554-567页

作者： li Weiming GAO Ming XUE Weili li Xiaoli CHANG Yu ZHANG Kaixin WEN Chenyu ZHANG Mingzhi Department of Oncology Henan University of Traditional Chinese MedicineZhengzhou 450000China Department of Oncology The First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450000China

Diffuse large B-cell lymphoma(DLBCL)is characterized by significant treatment *** acid(PA)has shown promising antitumor *** study aims to elucidate the molecular mechanisms by which PA influences DLBCL *** quantified the expression levels of microRNAs(miRNAs),Forkhead box protein O1(FOXO1),and DNA methyltransferase 3A(DNMT3A)in both untreated and PA-treated DLBCL tumors and cell *** were made of cell viability,apoptosis,and autophagy-related protein expression following PA *** analyses among miR-429,DNMT3A,and FOXO1 were conducted using luciferase reporter assays and methylation-specific(MSP)Polymerase chain reaction(PCR).After transfecting the miR-429 inhibitor,negative control(NC)inhibitor,shRNA against DNMT3A(sh-DNMT3A),shRNA negative control(sh-NC),over-expression vector for DNMT3A(oe-DNMT3A),or overexpression negative control(oe-NC),we evaluated the effects of miR-429 and DNMT3A on cell viability,mortality,and autophagy-related protein expression in PA-treated DLBCL cell *** efficacy of PA was also tested in vivo using DLBCL tumor-bearing mouse ***-429 and FOXO1 expression levels were downregulated,whereas DNMT3A was upregulated in DLBCL compared to the control *** treatment was associated with enhanced autophagy,mediated by the upregulation of miR-429 and downregulation of *** luciferase reporter assay and MSP confirmed that miR-429 directly inhibits DNMT3A,thereby reducing FOXO1 *** experiments demonstrated that PA promotes autophagy and inhibits DLBCL progression by upregulating miR-429 and modulating the DNMT3A/FOXO1 *** vivo PA signific-antly reduced the growth of xenografted tumors through its regulatory impact on the miR-429/DNMT3A/FOXO1 *** acid may modulate autophagy and inhibit DLBCL progression by targeting the miR-429/DNMT3A/FOXO1 signaling pathway,suggesting a novel therapeutic target for DLBCL management.

关键词： Palmitic acid Diffuse large B-cell lymphoma MiR-429 DNMT3A FOXO1 Autophagy

sh2B1 Tunes Hippocampal ERK Signaling to Influence Fluid Intelligence in Humans and Mice

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Research 2024年第3期2023卷 369-389页

作者： Xiujuan Du Yuhua Yan Juehua Yu Tailin Zhu Chu-Chung Huang lingli Zhang Xingyue shan Ren li Yuan Dai Hui LV Xiao-Yong Zhang Jianfeng Feng Wei-Guang li Qiang Luo Fei li Developmental and Behavioral Pediatric Department Brain and Behavioral Research Unit of Shanghai Institute for Pediatric Research and Ministry of Education-Shanghai Key Laboratory for Children's Environmental HealthXinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Developmental and Behavioral Pediatric Department Shanghai Xinhua Children's HospitalShanghai 200092China National Clinical Research Center for Aging and Medicine at Huashan Hospital Institute of Science and Technology for Brain-lnspired IntelligenceMinistry of Education-Key Laboratory of Computational Neuroscience and Brain-lnspired IntelligenceFudan UniversityShanghai 200433China State Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain Science Institutes of Brain Science and Human Phenom InstituteFudan UniversityShanghai 200032China shanghai Key Laboratory of Brain Functional Genomics(Ministry of Education) School of Life SciencesEast China Normal UniversityShanghai 200062China Department of Rehabilitation Medicine Huashan HospitalInstitute for Translational Brain ResearchState Key Laboratory of Medical Neurobiology and Ministry of Education Frontiers Center for Brain ScienceFudan UniversityShanghai 200032China Center for Experimental Studies and Research The First Affliated Hospital of Kunming Medical UniversityKunming 650032China shanghai Research Center for Brain Science and Brain-lnspired Intelligence Shanghai201210China Institute of Cognitive Neuroscience School of Psychology and Cognitive ScienceEast China Normal UniversityShanghai 200062China.

Fluid intelligence is a cognitive domain that encompasses general reasoning, pattern recognition, and problem-solving abilities independent of task-specific experience. Understanding its genetic and neural underpinnings is critical yet challenging for predicting human development, lifelong health, and well-being. One approach to address this challenge is to map the network of correlations between intelligence and other constructs. In the current study, we performed a genome-wide association study using fluid intelligence quotient scores from the UK Biobank to explore the genetic architecture of the associations between obesity risk and fluid intelligence. Our results revealed novel common genetic loci (sh2B1, TUFM, ATP2A1, and FOXO3) underlying the association between fluid intelligence and body metabolism. Surprisingly, we demonstrated that sh2B1 variation influenced fluid intelligence independently of its effects on metabolism but partially mediated its association with bilateral hippocampal volume. Consistently, selective genetic ablation of sh2b1 in the mouse hippocampus, particularly in inhibitory neurons, but not in excitatory neurons, significantly impaired working memory, short-term novel object recognition memory, and behavioral flexibility, but not spatial learning and memory, mirroring the human intellectual performance. Single-cell genetic profiling of sh2B1-regulated molecular pathways revealed that sh2b1 deletion resulted in aberrantly enhanced extracellular signal-regulated kinase (ERK) signaling, whereas pharmacological inhibition of ERK signaling reversed the associated behavioral impairment. Our cross-species study thus provides unprecedented insight into the role of sh2B1 in fluid intelligence and has implications for understanding the genetic and neural underpinnings of lifelong mental health and well-being.

关键词： metabolism impaired reversed

A dual adaptive unscented Kalman filter algorithm for SINS-based integrated navigation system

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Journal of Systems Engineering and Electronics 2024年第3期35卷 732-740页

作者： LYU Xu MENG Ziyang li Chunyu CAI Zhenyu HUANG Yi li Xiaoyong YU Xingkai Department of Precision Instrument Tsinghua UniversityBeijing 100084China College of Mechanical and Power Engineering Three Gorges UniversityYichang 443002China Unit 91001 of the PLA Beijing 100161China School of Control and Computer Engineering North China Electric Power UniversityBeijing 100096China

In this study, the problem of measuring noise pollution distribution by the intertial-based integrated navigation system is effectively suppressed. Based on nonlinear inertial navigation error modeling, a nested dual Kalman filter framework structure is developed. It consists of unscented Kalman filter (UKF)master filter and Kalman filter slave filter. This method uses nonlinear UKF for integrated navigation state estimation. At the same time, the exact noise measurement covariance is estimated by the Kalman filter dependency filter. The algorithm based on dual adaptive UKF (Dual-AUKF) has high accuracy and robustness, especially in the case of measurement information interference. Finally, vehicle-mounted and ship-mounted integrated navigation tests are conducted. Compared with traditional UKF and the Sage-Husa adaptive UKF (sh-AUKF), this method has comparable filtering accuracy and better filtering stability. The effectiveness of the proposed algorithm is verified.

关键词： Kalman filter dual-adaptive integrated navigation unscented Kalman filter(UKF) robust

Pien Tze Huang Inhibits Proliferation of Colorectal Cancer Cells through Suppressing PNO1 Expression and Activating p53/p21 Signaling Pathway

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Chinese Journal of Integrative Medicine 2024年第6期30卷 515-524页

作者： CAO liu-jing liU li-ya CHEN You-qin HAN Yu-ying WEI li-hui YAO Meng-ying FANG Yi WU Mei-zhu CHENG Ying Thomas J.Sferra liU Hui-xin li li PENG Jun shEN A-ling Clinical Research Institute the Second Affiliated Hospital&Academy of Integrative MedicineFujian University of Traditional Chinese MedicineFuzhou350122China Fujian Key Laboratory of Integrative Medicine in Geriatrics Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Pediatrics Case Western Reserve University School of MedicineUH Rainbow Babies and Children’s HospitalClevelandOH44106USA Innovation and Transformation Center Fujian University of Traditional Chinese MedicineFuzhou350122China Department of Health Management Fujian Provincial HospitalShengli Clinical College of Fujian Medical UniversityFuzhou000000China

ObjectiveTo explore the regulatory effect of Pien Tze Huang (PZH) on targeting partner of NOB1 (PNO1) and it’s down-stream mediators in colorectal cancer (CRC) *** polymerase chain reaction was performed to determine mRNA levels of PNO1, TP53, and CDKN1A. Western blotting was performed to determine protein levels of PNO1, p53, and p21. HCT-8 cells were transduced with a lentivirus over-expressing PNO1. Colony formation assay was used to detect cell survival in PNO1 overexpression of HCT-8 cells after PZH treatment. Cell-cycle distribution, cell viability and cell apoptosis were performed to identify the effect of PNO1 overexpression on cell proliferation and apoptosis of HCT-8 cells after PZH treatment. Xenograft BALB/c nude mice bearing HCT116 cells transduced with sh-PNO1 or sh-Ctrl lentivirus were evaluated. Western blot assay was performed to detect PNO1, p53, p21 and PCNA expression in tumor sections. Terminal deoxynucleotidyl transferase dUTP nick end labling (TUNEL) assay was used to determine the apoptotic cells in *** treatment decreased cell viability, down-regulated PNO1 expression, and up-regulated p53 and p21 expressions in HCT-8 cells (Plity, cell cycle arrest and cell apoptosis in vitro (Pliminated the effects of PZH treatment on tumor growth, inhibiting cell proliferation inhibition and apoptosis induction in vivo (Pliferative effect is at least in part by regulating the expression of PNO1 and its downstream targets p53 and p21.

关键词： colorectal cancer Pien Tze Huang Chinese medicine partner of NOB1 proliferation p53/p21 pathway

A judicious approach to induce large size growth of hydroxyapatite via applying graphene modified silicon nitride nanowires

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Journal of Materiomics 2024年第2期10卷 490-498页

作者： lina Sun Leilei Zhang Ruonan Zhang Hejun li State Key Laboratory of Solidification Processing Shanxi Key Laboratory of Fiber Reinforced Light Composite MaterialsNorthwestern Polytechnical UniversityXi'an710072China

Mimicking the structure of natural bone collagen fibers/hydroxyapatite(HA)to synthesize large size of HA for accelerated bone repair remains a ***,silicon nitride nanowires(SN)-graphene(GE)was designed by the chemical vapor deposition,forming SN-GE(SG)similar to collagen ***,the large size HA was assembled onto SG by pulsed electrochemical deposition,the SG/HA(SGH)mimics the collagen fibers/HA structure of *** introduction of SG induces HA to large size grow in the form of *** can be grown on a large size inextricably with the existence of GE modified *** the one hand,the upright GE sheets effectively increases the surface roughness which enhances the nucleation site of *** the other hand,the C■O provides chemical bonding and induces HA *** with SN/HA(sh),the porosity of SGH decreased by 71%.The average diameter of the SGH is(9.76±0.25)*** with sh,the diameter of SGH is 22 times larger than the diameter of *** that SG induces large size growth of *** work can provide a general strategy for the efficient preparation of biological scaffolds with large size HA that can be used in bone tissue engineering.

关键词： Hydroxyapatite Graphene Large size Silicon nitride nanowires Bone tissue engineering

An intrinsically self-healing and anti-freezing molecular chains induced polyacrylamide-based hydrogel electrolytes for zinc manganese dioxide batteries

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Journal of Energy Chemistry 2024年第2期89卷 565-578,I0013页

作者： Haiyang liao Wenzhao Zhong Chen li Jieling Han Xiao Sun Xinhui Xia Ting li Abolhassan Noori Mir F.Mousavi Xin liu Yongqi Zhang Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731SichuanChina School of Mechanical Engineering Hunan University of TechnologyZhuzhou 412007HunanChina School of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014ZhejiangChina Department of Chemistry Faculty of Basic SciencesTarbiat Modares UniversityTehran 14117-13116Iran State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200HubeiChina

The anti-freezing strategy of hydrogels and their self-healing structure are often contradictory,it is vital to break through the molecular structure to design and construct hydrogels with intrinsic anti-freezing/self-healing for meeting the rapid development of flexible and wearable devices in diverse service ***,we design a new hydrogel electrolyte(AF/sh-Hydrogel)with intrinsic anti-freezing/self-healing capabilities by introducing ethylene glycol molecules,dynamic chemical bonding(disulfide bond),and supramolecular interaction(multi-hydrogen bond)into the polyacrylamide molecular *** to the exceptional freeze resistance(84%capacity retention at-20℃)and intrinsic self-healing capabilities(95%capacity retention after 5 cutting/self-healing cycles),the obtained AF/sh-Hydrogel makes the zinc||manganese dioxide cell an economically feasible battery for the state-of-the-art *** Zn||AF/sh-Hydrogel||MnO_(2)device offers a near-theoretical specific capacity of 285 m A h g^(-1)at 0.1 A g^(-1)(Coulombic efficiency≈100%),as well as good self-healing capability and mechanical flexibility in an ice *** work provides insight that can be utilized to develop multifunctional hydrogel electrolytes for application in next generation of self-healable and freeze-resistance smart aqueous energy storage devices.

关键词： Flexible aqueous battery Hydrogel electrolyte Anti-freezing Self-healing Dual-dynamic reversible bond

Interaction mechanism between BaZrO_(3)/Y_(2)O_(3) refractory and Ti_(2)Ni alloy melts

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Journal of Iron and Steel Research International 2024年第6期31卷 1376-1388页

作者： Xiao Hou Guang-yao Chen Jian liu Ming-rui Lv Fei-hai Yu Qi-sheng Feng Rong-bin li Xiong-gang Lu Chong-he li State Key Laboratory of Advanced Special Steel and shanghai Key Laboratory of Advanced Ferromeallurgy and School of Materials Science and Engineering Shanghai UniversityShanghai 200072China shanghai Special Casting Engineering Technology Research Center Shanghai 201605China Zhejiang Institute of Advanced Materials Shanghai UniversityJiashan 314100ZhejiangChina School of Materials Science and Engineering Shanghai DianJi UniversityShanghai 201306China

The titanium alloys with highly chemical activity require stable crucible refractories that can withstand the erosion of alloy *** phase composition and microstructure are crucial factors that affect the stability of the refractory *** effect of Y_(2)O_(3) on the composition and microstructure of BaZrO_(3) crucible was systematically investigated,and the improved mechanism of the stability of BaZrO_(3)/Y_(2)O_(3) crucible was clarified in comparison with the BaZrO_(3) *** results showed that the erosion layer thickness of the BaZrO_(3)/Y_(2)O_(3) crucible was only 63μm,which was far less than that in the BaZrO_(3) crucible(485μm),and the erosion layer in the BaZrO_(3)/Y_(2)O_(3) crucible also exhibited a higher density than that in the BaZrO_(3) *** the sintering,Y_(2)O_(3) could improve the densification of the BaZrO_(3) crucible due to the solid solution effect between Y_(2)O_(3) and ZrO_(2),which also caused the evaporation of BaO,resulting in the generation of a Y_(2)O_(3)(ZrO_(2))film on the surface of the ***,the Y_(2)O_(3)(ZrO_(2))had higher thermodynamic stability than Y_(2)O_(3),confirming that the BaZrO_(3)/Y_(2)O_(3) crucible with high density exhibited a superior erosion resistance to titanium *** dual-phase structure provides a strategy to design a long-life and stable refractory for melting titanium alloys.

关键词： Barium zirconate Yttrium oxide Dual-phase refractory Titanium alloy Interaction

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产业链韧性测度方法研究进展

经济学动态 2024年第4期 144-160页

作者：肖兴志王振宇李少林东北财经大学产业组织与企业组织研究中心 116025

产业链韧性测度是识别“断链”风险和维护产业链安全稳定的现实抓手,成为新时代应对、把握和引领大变局亟待解决的热点问题。本文在分析产业链韧性测度现实需求的基础上,从产业链韧性测度的理论基础、方法类型、应用步骤三个方面就经典... 详细信息

产业链韧性测度是识别“断链”风险和维护产业链安全稳定的现实抓手,成为新时代应对、把握和引领大变局亟待解决的热点问题。本文在分析产业链韧性测度现实需求的基础上,从产业链韧性测度的理论基础、方法类型、应用步骤三个方面就经典文献进行述评。首先,围绕恢复、抵抗、适应与变革四大能力追溯概念界定、内涵特征和学科交叉的理论起源;其次,系统梳理了以核心变量法、综合评价法及投入产出法为代表的产业链韧性评价方法,剖析了各自优点与相对不足;最后,全面厘清了“冲击识别—结构分析—韧性评价”框架下的韧性测度理论与方法应用。本文认为,产业链韧性测度方法的选取及应用须高度重视学科交叉的应用场景,廓清理论模型的适用条件,为测度和增强产业链韧性提供多样化、稳健性的方法支撑。

关键词：产业链韧性经济韧性测度方法