Alternative relay regulates the adenosine triphosphatase activity of Locusta migratoria striated muscle myosin

作     者：Jie Hao Chang Liu Ning Zhang Jing Li Tong Ni Mingbo Qu Xiang-dong Li 

作者机构：State Key Laboratory oflntegrated Management of lnsect Pests and RodentsInsttute of ZoologyChinese Academy of SciencesBejingChina University of Chinese Academy of SciencesBejingChina School of BioengeeringDalian University of TechnologyDalianChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2024年第31卷第2期

页      面：435-447页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31672359 and 31970657) State Key Laboratory of Integrated Management of Pest Insects and Rodents(Grant No.IPM1601) 

主　　题：alternative splicing ATPase Locusta migratoria molecular motor muscle myosin 

摘      要：Locust(Locusta migratoria)has a single striated muscle myosin heavy chain(Mhc)gene,which contains 5 clusters of alternative exclusive exons and 1 differently included penultimate *** alternative exons of Mhc gene encode 4 distinct regions in the myosin motor domain,that is,the N-terminal SH3-like domain,one lip of the nucleotide-binding pocket,the relay,and the ***,we investigated the role of the alternative regions on the motor function of locust muscle *** Sf9-baculovirus protein expression system,we expressed and purified 5 isoforms of the locust muscle myosin heavy meromyosin(HMM),including the major isoform in the thorax dorsal longitudinal flight muscle(FL1)and 4 isoforms expressed in the abdominal intersegmental muscle(AB1 to AB4).Among these 5 HMMs,FL1-HMM displayed the highest level of actin-activated adenosine triphosphatase(ATPase)activity(hereafter referred as ATPase activity).To identify the alternative region(s)responsible for the elevated ATPase activity of FL1-HMM,we produced a number of chimeras of FL1-HMM and *** with the relay of AB4-HMM(encoded by exon-14c)substantially decreased the ATPase activity of FL1-HMM,and conversely,the relay of FL1-HMM(encoded by exon-14a)enhanced the ATPase activity of *** showed that the exon-14a-encoded residues Gly474 and Asn509 are responsible for the elevated ATPase activity of *** results indicate that the alternative relay encoded by exon-14a/c play a key role in regulating the ATPase activity of FL1-HMM and AB4-HMM.