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Relationship between atpase activity and conjugated polyamines in mito-chondrial membrane from wheat seedling roots under osmotic stress

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Journal of Environmental Sciences 2004年第5期16卷 712-716页

作者： LIUHuai-pan LIUJun ZHANGYan-yan LIUYou-liang KeyLaboratoryofCropGrowthRegulation MinistryofAgricultureCollegeofLifeScienceNanjingAgricultureUniversityNanjing210095China

The effects of osmotic stress on the atpase activity, the contents of —SH group and conjugated polyamines in mitochondrial membrane from wheat seedling [Triticum aestivum L. cv. Yumai No.18(drought-tolerant) and cv. Yumai No.9(drought-sensitive)] roots were investigated. The results showed that atpase activity and —SH group content decreased with polyethylene glycol(PEG) 6000(-0.55 MPa) treatment for 7 d, in concert with the decrease of the ratio of noncovalently conjugated spermidine(NCC-Spd)/noncovalently conjugated putrescine(NCC-Put) and increase of the covalently conjugated putrescine(CC-Put). Osmotic stress injury to Yangmai No.9 seedlings was alleviated greatly with 1 mmol/L exogenous spermidine(Spd), in concert with marked increases of the ratio of NCC-Spd/NCC-Put, —SH group contents and atpase activity in mitochondrial membrane. Under osmotic stress, the concomitant treatment of Yumai No.18 seedlings with methylglyoxyl bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosyl methionine decarboxylase(SAMDC), and phenanthrolin (o-Phen), an inhibitor of transglutaminase(TGase), caused a significant decrease of the ratio of NCC-Spd / NCC-Put, CC-Put contents, respectively, in concert with the marked decreases of atpase activity, —SH group content and its tolerance to osmotic stress. All the results above suggested that osmotic stress tolerance of wheat seedlings was associated with the atpase activity, the contents of —SH group, NCC-Spd and CC-Put in mitochondrial membrane.

关键词： atpase conjugated polyamines mitochondria osmotic stress wheat(Triticum aestivum L.)

Structural basis for a homodimeric atpase subunit of an ECF transporter

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Protein & Cell 2013年第10期4卷 793-801页

作者： Chengliang Chai You Yu Wei Zhuo Haifeng Zhao Xiaolu Li Na Wang Jijie Chai Maojun Yang College of Biological Sciences China Agricultural UniversityBeijing 100083China MOE Key Laboratory of Protein Sciences Tsinghua-Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China National Institute of Biological Sciences Beijing 102206China

The transition metal cobalt,an essential cofactor for many enzymes in prokaryotes,is taken up by several specifi c transport *** CbiMNQO protein complex be-longs to type-1 energy-coupling factor(ECF)transporters and is a widespread group of microbial cobalt *** is the atpase subunit(A-component)of the cobalt transporting system in the gram-negative thermo-philic bacterium Thermoanaerobacter *** we report the crystal structure of a nucleotide-free CbiO at a resolution of 2.3Å.CbiO contains an N-terminal canonical nucleotide-binding domain(NBD)and C-termi-nal helical *** and biochemical data show that CbiO forms a homodimer mediated by the NBD and the C-terminal *** mainly via conserved hydrophobic amino acids between the two C-terminal do-mains result in formation of a four-helix *** comparison with other ECF transporters suggests that non-conserved residues outside the T-component bind-ing groove in the A component likely act as a specifi city determinant for T ***,our data provide information on understanding of the structural organiza-tion and interaction of the CbiMNQO system.

关键词： CbiO Cobalt ECF atpase Thermoanaero-bacter tengcongensis

Rice Heavy Metal P-type atpase OsHMA6 Is Likely a Copper Efflux Protein

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Rice science 2020年第2期27卷 143-151页

作者： ZOU Wenli LI Chang ZHU Yajun CHEN Jingguang HE Haohua YE Guoyou Group of Crop Genetics and Breeding School of Agriculture ScienceJiangxi Agricultural UniversityNanchang 330045China CAAS-IRRI Joint Laboratory for Genomics-Assisted Germplasm Enhancement Agricultural Genomics Institute in ShenzhenChinese Academy of Agricultural SciencesShenzhen 518057China College of Resources and Environment Henan Agricultural UniversityZhengzhou 450002China

P1B-type heavy metal atpases(HMAs)are transmembrane metal-transporting proteins that play a key role in metal *** here reported the characterization of rice OsHMA6,a member of the P1B-type atpase *** tree analysis showed that OsHMA6 belonged to the Cu/Ag subgroup of the HMA family and had a close evolutionary relationship with *** acid sequence alignment showed 82.78%consistency between OsHMA6 and ***6 expressed in all organs at different growth stages,including spikelet,and abundant in leaf blades,however,OsHMA9 most strongly expressed in roots,but very low in *** Cu^2+can up-regulate the expression of OsHMA6 and OsHMA9 in rice *** heterologous expression in yeast showed that OsHMA6 can significantly rescue the growth of yeast strain CM52 when supplied with 3 or 6 mmol/L Cu^2+.Compared with the empty vector pYES2,the Cu concentration in OsHMA6-pYES2 decreased by 23.4%and 30.3%under 3 or 6 mmol/L Cu2+,*** localization revealed that OsHMA6 was located in the plasma *** results suggested that OsHMA6,similar to OsHMA9,is likely a copper efflux protein located in the plasma membrane.

关键词： OsHMA6 P1B-type atpase copper cadmium efflux rice subcellular localization

Comparative expression analysis of heavy metal atpase subfamily genes between Cd-tolerant and Cd-sensitive turnip landraces

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Plant Diversity 2019年第4期41卷 275-283页

作者： Yuansheng Wu Xiong Li Di Chen Xi Han Boqun Li Yonghong Yang Yongping Yang China Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China College of Plant Protection Yunnan Agricultural UniversityKunming 650201China Key Laboratory for Plant Diversity and Biogeography of East Asia Kunming Institute of BotanyChinese Academy of SciencesKunming 650201China School of Life Sciences Yunnan UniversityKunming 650091China

The heavy metal atpase(HMA)subfamily is mainly involved in heavy metal(HM)tolerance and transport in plants,but an understanding of the definite roles and mechanisms of most HMA members are still *** the present study,we identified 14 candidate HMA genes named BrrHMAl—BrrHMA8 from the turnip genome and analyzed the phylogeny,gene structure,chromosome distribution,and conserved domains and motifs of HMAs in turnip(Brassica rapa ***).According to our phylogenetic tree,the BrrHMAs are divided into a Zn/Cd/Co/Pb subclass and Cu/Ag *** BrrHMA members show similar structural characteristics within *** explore the roles of BrrHMAs in turnip,we compared the gene sequences and expression patterns of the BrrHMA genes between a Cd-tolerant landrace and a Cd-sensitive *** BrrHMA genes showed similar spatial expression patterns in both Cd-tolerant and Cd-sensitive turnip landraces;some BrrHMA genes,however,were differentially expressed in specific tissue in Cd-tolerant and Cd-sensitive ***,BrrHMA genes in the Zn/Cd/Co/Pb subclass shared the same coding sequence but were differentially expressed in Cd-tolerant and Cd-sensitive turnip landraces under Cd *** findings suggest that the stable expression and up-regulated expression of BrrHMA Zn/Cd/Co/Pb subclass genes under Cd stress may contribute to the higher Cd tolerance of turnip landraces.

关键词： Heavy metal atpase Cadmium Turnip Transmembrane helix Differential gene expression

Mg2+-MEDIATED CHANGE IN LIPID FLUIDITY ENHANCES THE RECONSTITUTED H+-atpase ACTIVITY

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Science in China,Ser.B 1983年第10期26卷 1046-1056页

作者：杨福愉郭倍奇王大辉 Institute of Biophysics Academia Sinica Beijing

The results by using ANS fluorescent probe and spin labels 5-NS show that the fluidity of L.（H+-atpase）+Mg2+ (H+-atpase from pig heart mitochondria reconstituted in the presence of Mg2+) is less than that of L （H+-atpase）-Mg2+ (proteoliposome reconstituted in the absence of Mg2+). But no significant difference in fluidity has been observed when both reconstituted systems were monitored by using spin labels 12-NS and 16-NS. This indicates that Mg2+ may cause changes in fluidity of the lipid molecules near the surfaces of the bilayers, but does not affect significantly the fluidity of the deeper layer of the reconstituted *** is tentatively supposed that in the presence of Mg2+, enhancement of activities of reconstituted H+-atpase may be due to the Mg2+-mediated change in physical state of the lipids in the more superficial region of lipid bilayers so as to ensure a suitable conformation of atpase complex, thereby possessing higher activity.

关键词： NS atpase ACTIVITY MEDIATED CHANGE IN LIPID FLUIDITY ENHANCES THE RECONSTITUTED H Mg atpase

Gene expression of 49 kDa apyrase,cytoskeletal proteins,atpase,ADPase and amino acid contents of Pisum sativum(L.)cells germinated in Euryops arabicus(*** Jaub.&Spach)water extract

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BIOCELL 2019年第2期43卷 81-87页

作者： MAHMOUD MOUSTAFA SAAD ALAMRI HOIDA ZAKI NAGLAA LOUTFY TAREK TAHA ALI SHATI MOHAMED ALKAHTANI SAJDA SIDDIQI Department of Biology College of ScienceKing Khalid University9004AbhaKingdom of Saudi Arabia(KSA) Department of Botany Faculty of ScienceSouth Valley UniversityQenaEgypt Environmental Biotechnology Department Genetic Engineering and Biotechnology Research Institute(GEBRI)City of Scientific Research and Technological Applications(SRTA-City)New Borg El-Arab City21934AlexandriaEgypt

The present research reports of quick and marked changes induced by plant extract of Euryops arabicus in the gene expression of 49-kDa apyrases,cytoskeletal proteins,atpases,ADPase and amount of amino acid of pea(Pisum sativum ***).Pellets of cytoskeletals proteins(27000 xg)were probed with anti-apyrase antibody,biotinylated anti-rat,actin and alpha and beta-tubulin for Western *** and ADPase activities were determined based on the hydrolytic efficacy of adenine triphosphate and adenine *** 72 hours,the abundance of apyrases,cytoskeletal proteins and amount of amino acid in pellets of 27000 xg of germinated pea seeds in *** extracts were sharply increased than those sown in distilled *** the samples exhibited that the stems had more amount from apyrases,cytoskeletal proteins,amino acids and atpase and ADPase activities than primary leaves and primary roots that were germinated either on *** water extract or in distilled *** on the enzyme’s capability to hydrolyse nucleotide triphosphate and nucleotide diphosphate as well as the direct association between expression of 49-kDa apyrase and cytoskeletal proteins,*** water extract had an important effect on plant germinations.

关键词： Cytoskeletal proteins atpase ADPase Pisum sativum Euryops arabicus

SUBUNIT STOICHIOMETRY AND INTERACTION OF F1-atpase OF BEEF HEART MITOCHONDRIA

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Science in China,Ser.B 1988年第3期31卷 319-327页

作者：刘树森 Department of Cell Biology Institute of Zoology Academia Sinica Beijing

By incubating soluble F1 of the beef heart mitochondria with radio active 7-chloro-4-nitro-2, 1, 3-benzoxadiazole (14C)-NBD-C1 in the dark, a labeled F1 with NBD/F1 molar ratio of 1: 1 （0.995: 1） wasobtained, but 98% of the atpase activity was inhibited. The inactivated atpase activity of the NBD-labeled F1 may be restored by DTT, suggesting that an essential tyrosine residue of F1 （O-NBD-F1） maybe labeled. By exposure of O-NBD-F1 to reflected light, the NBD label can be transferred to an aminogroup, forming an N-NBD-F1 with 0.83 NBD label per F1 molecule and a stable 82.5% inhibition ofthe atpase activity even in the presence of DTT. Urea PAGE analysis clearly showed that a 63.4% ofthe total radio activity was associated with the β-subunit. In the β-fraction from N-NBD-F1 only a 19.9-20% of the labeling was detected. It is calculated that F1 of the beef heart mitochondria contains 3（2.63-2.64） β-subunits. The results obtained are discussed in connection with the subunit-subunit andsite-

关键词： NBD SUBUNIT STOICHIOMETRY AND INTERACTION OF F1-atpase OF BEEF HEART MITOCHONDRIA atpase

STUDY ON THE INTERACTION OF Se AND ERYTHROCYTE MEMBRANE——EFFECT OF Se ON THE Na, K-atpase ACTIVITY AND FLUIDITY OF ERYTHROCYTE MEMBRANES

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Science China Chemistry 1986年第10期29卷 1077-1083页

作者：杨福愉沃维汉 Institute of Biophysics Beijing Academia Sinica

The content of binding-Se in erythrocyte membrane may affect its structure and function. Decrease in So content will lead to a lower activity of Na, K-atpase and lipid fluidity in ghosts, and a marked increase in Na, K-atpase activity as well as lipid fluidity of erythrocyte membrane was observed by supplementation of low concentration of NaSeO（0.03—0.3 ppm Se）, in vitro. This effect was obvious only when the ghosts wore incubated with Se at 0—4°C for at least half an hour prior to assay.

关键词： EFFECT OF Se ON THE Na K-atpase ACTIVITY AND FLUIDITY OF ERYTHROCYTE MEMBRANES STUDY ON THE INTERACTION OF Se AND ERYTHROCYTE MEMBRANE atpase

Potentiating activity of luteolin on membrane permeabilizing agent and atpase inhibitor against methicillin-resistant Staphylococcus aureus

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Asian Pacific Journal of Tropical Medicine 2016年第1期9卷 16-19页

作者： Dae-Ki Joung Young-Seob Lee Sin-Hee Han Sang-Won Lee Seon-Woo Cha Su-Hyun Mun Ryong Kong Ok-Hwa Kang Ho-Jun Song Dong-Won Shin Dong-Yeul Kwon Department of Oriental Pharmacy College of Pharmacy and Wonkwang-Oriental Medicines Research Institute.Wonkwang University Department of Herbal Crop Research National Institute of Horticultural & Herbal ScienceRDA BK21 Plus Team Professional Graduate School of Oriental MedicineWonkwang University Department of Oriental Medicine Resources Sunchon National University

Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent atpase inhibitors,and peptidoglycan(PGN) derived from Staphylococcus aureus(***).Also,transmission electron microscopy was used to monitor survival characteristics and changes in *** ***:Compared to the LUT alone,the optical density of suspensions treated with the combination of 125 μg/mL Tris and 230 μg/mL DCCD were reduced to 60%and 46%,***(15.6 μg/mL) gradually impeded the activity of LUT,and PGN(62.5 μg/mL) completely blocked the activity of LUT on ***:Increased susceptibility to LUT with me Tris and DCCD combinations is evident in all tested MRSA *** results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting *** PGN directly blocks the antibacterial activity of LUT,suggesting the direct binding of LUT with *** findings may be validated for the development of antibacterial agent for low MRSA resistance.

关键词： Iuteolin Methicillin-resistant Staphylococcus aureus Membrane permeabilizing agent atpase inhibitor Peptidoglycan