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Mutation analysis of the checkpoint kinase 2 gene in colorectal cancer cell lines

Chinese Medical Journal 2007年第23期120卷 2119-2123页

作者： LIU Wei-dong ZHONG Bai-yun ZHANG Yang-de choi Gyu-seog National Hepatobiliary and Enteric Surgery Research Center Ministry of Health Xiangya Hospital Central-south University Changsha 410008 China Division of Colorectal Surgery Department of Surgery Bio-medical Research Institute Kyungpook National University Hospital Daegu 700412 Korea

Background Checkpoint kinase 2 （CHK2） is a DNA damage-activated protein kinase which is involved in cell cycle checkpoint control, CHK2 gene could be a candidate gene for colorectal cancer susceptibility, But there are few systematic reports on mutation of CHK2 in colorectal cancer. Methods The mutations of all 14 exons of CHK2 in 56 colorectal cancer cell lines were screened systematically, using denaturing high-performance liquid chromatography （DHPLC） to screen the mismatches of the CHK2 exons amplified products, and then the suspected mutant cell lines were scanned by nucleotide sequence analysis. Results VACO400 in CHK2 exon la was suspected to have mutation by DHPLC and confirmed by sequence, but this was nonsense mutation. C106, CX-1, HT-29, SK01, SW480, SW620 and VACO400 in CHK2 exon lb were confirmed to have the same nonsense mutation in 11609 A〉G. DLD-1 and HCT-15 in CHK2 exon 2 were confirmed to have missense mutation R145W, which was heterozygous C〉T missense mutation at nucleotide 433, leading to an Arg〉Trp substitution within the FHA domain. Conclusions The CHK2 mutation in colorectal cancer is a low frequency event, There are just 10 cell lines to have sequence variations in all the 14 exons in 56 colorectal cancer cell lines and only DLD-1/HCT-15 had heterozygous missense mutation. These findings may give useful information of susceptibility of colorectal cancer as single nucleotide polvmorphvsim.

关键词： checkpoint kinase 2 colorectal cancer denaturing high-performance liquid chromatography cell line, colorectal cancer

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Endothelial progenitor cell differentiation using cryopreserved,umbilical cord blood-derived mononuclear cells

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Acta Pharmacologica Sinica 2007年第3期28卷 367-374页

作者： Jun-ho JANG~(2,5) Sun-kyung KIM~(3,5) Jeong-eun choi~3 young-jin KIM~3 Hyun-woo LEE~2 Seok-yun KANG~2 Joon-seong PARK~2 Jin-hyuk choi~2 Ho-yeong LIM~4 Hugh C KIM~(2,6)2 Department of Hematalogy-Oncology,Ajou University,School of Medicine,Suwon 442749,Korea 3 Biomedical Research Institute,LifeCord,Suwon 442749,Korea 4 Division of Hematology-Ontology,Department of Medicine,Samsung Medical Center,Sungkyunkwan University School of Medicine,Seoul 135710,Korea Department of Hematology-Oncology Ajou University School of Medicine Suwon Korea Department of Biomedical Research Institute Suwon Korea Division of Hematology-Oncology Department of Medicine Samsung Medical Center Sungkyunkwan University School of Medicine Seoul Korea

Aim:To investigate the endothelial differentiation potentiality of umbilical cordblood(UCB),we induced the differentiation of endothelial progenitor cells(EPC)from cryopreserved UCB-derived mononuclear cells(MNC).Methods:MNC fromcryopreserved UCB and peripheral blood(PB)were cultured in M199 medium withendothelial cell growth supplements for 14 *** were characterized by RT-PCR,flow cytometry,and immunocytochemistry *** proliferation of differen-tiated EPC was studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide(MTT)assay,and vascular endothelial growth factor(VEGF)concentra-tion was measured using an ELISA *** of UCB-derived EPC werecompared with those of PB-derived ***:A number of round-shapedcells were loosely attached to the bottom after 24 h culture,and numerous spindle-shaped cells began to appear from the round-shaped ones on d *** cellsexpressed endothelial markers such as,Flt-1/VEGFR-1,ecNOS,VE-cadherin,vonWillebrand factor,and secreted VEGE The patterns of endothelial markers of EPCfrom PB and UCB did not show striking *** results of the prolifera-tion and secretion of VEGF were also ***:We successfully cul-tured UCB cells stored at -196℃ into cells with the quality of endothelial *** EPC could be used for angiogenic therapeutics by activating adjacentendothelial cells and enhancing angiogenesis.

关键词： cord blood endothelial cells cryopreserved

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韩国跆拳道教练员指导行为分析

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军事体育进修学院学报 2010年第4期29卷 52-54页

作者：焉石崔宽容崔永俊哈尔滨工程大学体育军事训练部黑龙江哈尔滨150001 韩国体育大学

通过运用Arizona大学的观察体系方法,对韩国跆拳道教练员在训练过程中的指导行为进行了分析归纳。结果表明:韩国跆拳道教练员在平时训练时的指导行为大致可以归纳为几个方面:技术指导、动作意见反馈、选手管理等方面。通过归纳分析跆拳... 详细信息

通过运用Arizona大学的观察体系方法,对韩国跆拳道教练员在训练过程中的指导行为进行了分析归纳。结果表明:韩国跆拳道教练员在平时训练时的指导行为大致可以归纳为几个方面:技术指导、动作意见反馈、选手管理等方面。通过归纳分析跆拳道强国韩国队教练员的指导行为,为我国的跆拳道项目日后更好地发展提供参考。

关键词：跆拳道指导行动

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STANDARDIZATION OF LED-BASED FLAT PANEL FOR USE IN GENERAL LIGHTING

STANDARDIZATION OF LED-BASED FLAT PANEL FOR USE IN GENERAL L...

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The First Tripartite Lighting SCI-TECH Forum of China,Japan and Republic of Korea

作者： Sang-Kyoo Jeon Mee-Ryoung Cho Suk-Joon choi Jae-Yeop Rho Se-Hyun Lee Sang-Wuk Shin Myung-Keun Hwang Do-young Lee Seong-Yong Yang Korea Institute of Lighting Technology 187-7 Dodang-dong Wonmi-guBucheon-cityGyeonggidoSouth Korea

＜正＞Many measurement methods for white LEDs have been studied in the filed of lighting,extensively, since it has developed and used as the wide range of ***,the studies of standardization of LED-based flat panel for general lighting have not been studied a *** this paper,we present and analyze the applicable standards of LED-based flat panel for use in general lighting.

关键词： Light Emitting Diode (LED) General Lighting Flat Panel Standard

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Disease-specific survival in patients with renal cell carcinoma an audit of a large series from Korea

Disease-specific survival in patients with renal cell carcin...

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第十七届全国泌尿外科学术会议

作者： Ja Hyeon Ku Soo Woong Kim Jae-seung Paick Department of Urology Seoul National University College of Medicine

Background To evaluate the question of whether or not young age is an independent prognostic factor for disease-specific survival in Korean patients with renal cell carcinoma(RCC). Methods A total of 785 patients with...

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Molecular Dynamic Studies on Mechanical Properties of Poly crystalline Metal in Nano Indentation

Molecular Dynamic Studies on Mechanical Properties of Poly c...

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The Fourth International Conference on Physical and Numerical Simulation of Materials (ICPNS’2004)

作者： Junwon Park youngsuk Kim Seogou choi

＜正＞The objective of this study is the use of MD simulation to evaluate the effect of grain size on nanoindentation properties. The Morse potential is employed to model the interatomic forces between atoms. young’s modulus and the hardness of the workmaterials are evaluated from load-depth curve obtained in indentation simulation. The relationship between temperature and the mechanical properties, and the influence of grain size on the yield behaviors of metal have been studied by applying to the Hall-Petch relation. MD simulation results are compared with several models results for load-depth curve and hardness. Finally, this simulation results are compared with the experimental data obtained from the literatures

关键词： Nano indentation Polycrystalline Molecular Dynamic

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5-fluorouracil,Methotrexate,Leucovorin,CDDP and Epirubicin（FEPMTX）:A Wide-Spectrum Regimen of Salvage Chemotherapyfor High- Grade Advanced Gastric Cancer

5-fluorouracil,Methotrexate,Leucovorin,CDDP and Epirubicin（...

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2000全国肿瘤学术大会

作者： Kazuo Kinoshita Yutaka Yonemura Toshiharu Sawa Tatsukazu Miyata Hiroshi Sakuma Nobuo Matsuki Sigehiro Tanaka Sachio Fushida Takashi Fujimura young Jae Mok Byung Sik Kim Jong In Lee Dong Wook choi young Jin Song Doo Hyun Yang Sung Joon Kwon Itsuo Miyazaki Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Department of Surgery Ⅱ School of medicineKanazawa University13-1 Takara-machiKanazawaJapan M.D. Korea University Hospital M.D. Ulsan University Hospital M.D. Korean Cancer Center Hospital M.D. Korean Cancer Center Hospital M.D. Chungbuk National University Hospital M.D. Chonbuk National University Hospital M.D. Hanyang University Hospital Korea M.D. Department of Surgery Ⅱ School of medicine Kanazawa University13-1 Takara-machiKanazawaJapan M.D.

＜正＞Twenty-one patients with primary high-grade advanced gastric cancer in whom the presence of stage ? was con- firmed by preoperative diagnostic imaging,were treated with a wide-spectrum regimen,designated as FEPMTX therapy to establish an effective salvage *** therapy consisted of 5-fluorouracil（5-FU）and the triple biochemical modulators;methotrexate（MTX）,calcium leucovorin（LV）,cisplatin（CDDP）,and in addition of epiru- bicin（EPI）.The schedule comprised 3 days continuous administration of 5-FU（350 mg/m2/day）and MTX（35 mg/ m2）on day 1,LV（30 mg/m2）on the day 2,LV（30 mg/m2）and CDDP（30 mg/m2）and EPI（20 mg/m2）on day *** treatment was performed two or more courses with 2 weeks interval in *** partial responses（PR）, five no changes（NC）and five progressive diseases（PD）were obtained,and the response rate was 52%.Ten patients （PR 7,NC 2,PD 1）were received gastrectomy（resectability rate 48%）.The survival in all cases resulted in median survival of 184 days and one-year survival rate of 23%.While there is no significant difference insurvival between the cases with or without the resection of the primary lesion,the survival of responders was significantly longer than that of non-responders（median survival time;responder 356days,non-responder 152days）.Adverse effects such as myelo- suppression and anorexia and fatigue sometimes occurred,but were mild and all the patients well *** therapy is thought to be an effective regimen for neoadjuvant chemotherapy with longer survival and little toxic for patients with stage?gastric cancer.

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Characteristic Dermatologic Changes in the Condition of Skin after Ultrasonic Stimulation in Healthy Korean Men

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Journal of Cosmetics, Dermatological Sciences and Applications 2018年第4期8卷 195-206页

作者： So-Jung Kim seung-Min Yang Ju-Hyun Kim Jeong-Uk Lee Mee-young Kim Lim-Kyu Lee Byoung-Sun Park Won-Deok Lee Ji-Woong Noh Yong-Sub Shin Doo-Ho Kim Il-Hyun Kim Junghwan Kim Laboratory of Health Science & Nanophysiotherapy Department of Physical Therapy Graduate School Yongin University Yongin Republic of Korea Department of Physical Therapy College of Health Welfare Wonkwang Health Science University Iksan Republic of Korea Department of Physical Therapy College of Health Science Honam University Gwangsan-gu Republic of Korea The Team of Safety and Quality Management Yeongwol Combined Cycle Power Plant Division Korea Southern Power Plant Yeongwol-gun Republic of Korea Department of Physical Therapy College of Public Health & Welfare Yongin University Yongin Republic of Korea

The aim of our study was to recognize different skin conditions of the face and neck using six biophysical parameters and to show the changes after the application of ultrasonic stimulation (US). We assessed the differences in the sebum, moisture, pores, wrinkles, pigmentation, and elasticity of the skin in the face and neck regions. A total of 30 healthy men in their 20s were enrolled. We used a skin diagnosis meter to assess the state of the skin. The sebum and pores of the face were more significant than in the neck. The amount of moisture in the U-zone was significantly higher than in other areas. Statistical differences were found between the pre-and post-measure-ment values in the sebum, moisture, wrinkles and pigmentation. Wrinkles and pigmentation were positively correlated. Our data showed that the changes in skin condition are associated with external stimulants. The effect of US on skin may differ depending on the part of the body, and some biophysical properties of skin vary depending on the location on the body.

关键词： Ultrasonic Stimulation Korean Men Skin

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Analysis of Skin Parameters of Korean Men According to the Parts of the Body for Integumentary Physiotherapy Research

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Health 2018年第4期10卷 467-486页

作者： So-Jung Kim Ju-Hyun Kim Jeong-Uk Lee Mee-young Kim Lim-Kyu Lee Byoung-Sun Park seung-Min Yang Won-Deok Lee Ji-Woong Noh Yong-Sub Shin Doo-Ho Kim Il-Hyun Kim Junghwan Kim Laboratory of Health Science & Nanophysiotherapy Department of Physical Therapy Graduate School Yongin University Yongin South Korea Department of Physical Therapy College of Health Welfare Wonkwang Health Science University Iksan South Korea Department of Physical Therapy College of Health Science Honam University Gwangju South Korea The Team of Safety and Quality Management Yeongwol Combined Cycle Power Plant Division Korea Southern Power Plant Busan South Korea Department of Physical Therapy College of Public Health & Welfare Yongin University Yongin South Korea

Studies have demonstrated that some cutaneous biophysical properties vary with the part of the body. The results to date of skin conditions in human skin of multiple parts of the body have not yet been well established. In this study, we assessed the differences in the skin’s sebum, moisture, pores, wrinkles, pigmentation, and elasticity of each body part in Korean men in their 20s. A total of 34 healthy men were enrolled. A Skin Diagnosis Meter was used to measure the skin’s surface sebum, moisture, pores, wrinkles, pigmentation, and elasticity of each body part. The sebum content was significantly higher on the face than at other sites. Moisture was significantly high on the feet. Pores were significantly high on the face. Wrinkles were significantly high on the face. Pigmentation was high on the face and neck, but not significantly. Elasticity was significantly high on the hands. In the correlation analysis results, sebum and pore were positively correlated, but sebum and moisture and wrinkle and elasticity were negatively correlated. For nearly the first time, this study resulted in systematic reference values for standardized biophysical measuring methods and body parts reflecting the skin physiology of healthy South Korean men. The results show that skin’s surface sebum, moisture, pores, wrinkles, pigmentation, and elasticity vary with the part of the body.

关键词： Sebum Moisture Pores Wrinkles Pigmentation Elasticity Total Body

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The Heart Disorders with Survey Study in Oriental Medicine: Using the Magnetocardiography (MCG)

The Heart Disorders with Survey Study in Oriental Medicine: ...

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第三届国际传统医药大会

作者： Sun Mi choi Yeon Hee Ryu Jin Suk Moon Joung Yeol Kim Kyung Mo Park young Huh Chan Gil Jeung Hyun Jea Sung Yoo sik Yoon Kyoo Seok Ahn Medical Research & Development Team Korea Institute of Oriental Medicine Department of biomedical Engineering Kyunghee University Korea Electrotechnology Research Institute College of Oriental Medicine Semyung University Kyunghee University Oriental Medical College Pathology Laboratory

Objective:This study was to determine the standard of criteria for each different diagnosis of signs and symptoms. Methods: Eleven experts were asked to evaluate the adequateness of criteria that was developed by Korea Institute of Oriental Medicine (KIOM) and suggested the amendment of them. To perform the study, we used the questionnaire which asks about the diagnosis criteria for an insufficiency of the heart-qi (心气虚证), deficiency of the heart blood (心血虚证), deficiency of the heart-yin (心阴虚证), insuffi-

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