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Potato Responds to Salt Stress by Increased Activity of Antioxidant Enzymes

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Journal of Integrative Plant Biology 2009年第12期51卷 1095-1103页

作者： Keyvan Aghaei Ali Akber Ehsanpour setsuko Komatsu National Institute of Crop Science Tsukuba 305-8518 Japan Department of Biology Faculty of Sciences University of Isfahan Isfahan *** Iran

To understand the response of potato to salt stress, antioxidant enzyme activities and ion content were analyzed for a sensitive and a tolerant cultivar. Nodal cuttings of the tolerant cultivar, Kennebec, and the sensitive cultivar, Concord, were exposed to media without or with 30, 60, 90 or 120 mmol/L NaCI for 4 weeks. On exposure to NaCI, the length and fresh and dry weight of both shoots and roots of Concord showed greater decrease than those of Kennebec. The decrease in shoot growth was more severe than that of the root for both cultivars. The K^＋ content of shoots and roots of both cultivars was reduced in a dose-dependent manner by exposure to NaCl; the Na^＋ content increased. Activities of ascorbate peroxidase, catalase and glutathione reductase were increased in NaCl-exposed shoots of Kennebec; the corresponding activities in NaCI-exposed shoots of Concord were decreased. Roots of both cultivars showed similar changes in the activities of these enzymes on exposure to NaCI. These studies established that enzyme activities in Concord shoots are inversely related to the NaCI concentration, whereas those in Kennebec do not show a dose dependency, which is also the case for the roots of both cultivars. Our findings suggest that an increase in activity of antioxidant enzymes, such as ascorbate peroxidase, catalase and glutathione reductase, can contribute to salt tolerance in Kennebec, a salt resistant cultivar of potato.

关键词： antioxidant enzyme ion content potato salt resistant cultivar salt stress.

PROTEOME ANALYSIS OF RICE ROOT PROTEINS REGULATED BY GIBBERELLIN

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Genomics, Proteomics & Bioinformatics 2005年第3期3卷 132-142页

作者： setsuko KOMATSU HIROSATO KONISHI DEPARTMENT OF MOLECULAR GENETICS NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES TSUKUBA 305-8602 JAPAN

To gain an enhanced understanding of the mechanism by which gibberellins （GAs） regulate the growth and development of plants, it is necessary to identify proteins regulated by GA. Proteome analysis techniques have been applied as a direct, effective, and reliable tool in differential protein expressions. In previous studies, sixteen proteins showed differences in accumulation levels as a result of treatment with GA3, uniconazole, or abscisic acid （ABA）, and/or the differences between the GA-deficient semi-dwarf mutant, Tan-ginbozu, and normal cultivars. Among these proteins, aldolase increased in roots treated with GA3, was present at low levels in Tan-ginbozu roots, and decreased in roots treated with uniconazole or ABA. In a root elongation assay, the growth of aldolase-antisense transgenic rice was half of that of vector control transgenic rice. These results indicate that increases in aldolase activity stimulate the glycolytic in the GA-induced growth of roots. In among GA, aldolase, and root growth. pathway and may play an important role this review, we discuss the relationship among GA, aldolase, and root growth.

关键词： aldolase gibberellin proteome rice

Functional Characterization of Gibberellin-Regulated Genes in Rice Using Microarray System

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Genomics, Proteomics & Bioinformatics 2006年第3期4卷 137-144页

作者： Asad Jan setsuko Komatsu National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan National Institute of Agrobiological Sciences Tsukuba 305-8602 Japan National Institute of Crop Science Tsukuba 305-8518 Japan.

Gibberellin （GA） is collectively referred to a group of diterpenoid acids, some of which act as plant hormones and are essential for normal plant growth and development. DNA microarray technology has become the standard tool for the parallel quantification of large numbers of messenger RNA transcripts. The power of this approach has been demonstrated in dissecting plant physiology and development, and in unraveling the underlying cellular signaling pathways. To understand the molecular mechanism by which GA regulates the growth and development of plants, with reference to the monocot model plant-rice, it is essential to identify and analyze more genes and their products at the transcription and translation levels that are regulated by GA. With the availability of draft sequences of two major rice types, indica and japonica rice, it has become possible to analyze global expression profiles of genes on a genome scale. In this review, the progress made in finding new genes in rice leaf sheath using microarray system and their characterization is discussed. It is believed that the findings made in this regard have important implications for understanding the mechanism by which GA regulates the growth and development of rice.

关键词： gibberellin gene expression microarray rice

Experiences of patients with HIV/AIDS receiving mid- and long-term care in Japan:A qualitative study

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International Journal of Nursing Sciences 2017年第2期4卷 99-104页

作者： Yoko Imazu Nao Matsuyama Sanae Takebayashi Mizue Mori setsuko Watabe Tokyo Medical and Dental University TokyoJapan Yokohama City University Hospital YokohamaJapan Yokohama City University YokohamaJapan Department of Nursing Graduate School of MedicineYokohama City University Yokohama236-0004Japan

Purpose: In the era of antiretroviral treatment (ART), treatment of HIV has become more manageable, and most patients with HIV benefit from long-term therapy in Japan. The aim of this qualitative study was to examine the experiences of patients with HIV/AIDS receiving mid-and long-term care in Japan. Methods: A qualitative study was performed using semi-structured interviews at an outpatient clinic of a university hospital in the Greater Tokyo area. A total of 31 Japanese patients with HIV who had been receiving treatment for at least a year were interviewed in Japanese. Data from these interviews were analyzed by'content analysis' (Krippendorff, 1980). Results: The data were organized into the following seven themes:"feelings toward diagnosis of HIV infection";"perceptions and behavior after diagnosis of HIV";"attitudes toward HIV therapy";"fear and hope for the future";"feelings toward professional support";"life changes after HIV therapy";and"struggles in relationships with others". Conclusions: Some participants accepted themselves as HIV-positive, some did not when they were diagnosis. This difference of reaction had effect on attitudes toward HIV therapy. But all participants continued mid- and long-treatments owing to feeling well and receiving support from professionals. Additionally, it found that patients were felt stigma of HIV-positive similarly to another Asian countries. Therefore, we should provide the entire Japanese society with knowledge about HIV/AIDS and be early and constant intervention by professional teams after being infected.

关键词： patients qualitative continued

Vowel Reduction in Japanese

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南开语言学刊 2009年第2期 14-20页

作者： Shirai setsuko MingDao University

This paper reports the result that vowel reduction occurs in Japanese and vowel reduction is the part of the language *** with English,the effect of the vowel reduction in Japanese is relatively weak might because of the absence of stress in *** spectral vowel reduction occurs in Japanese,various types of researches would be possible.

关键词： vowel reduction Japanese experimental analysis

Rice proteome analysis: a step toward functional analysis of the rice genome

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Rice proteome analysis: a step toward functional analysis of...

Is the Walking Campaign Effective for Depressive Symptoms?

首届全国蛋白质组学学术研讨会

作者： setsuko Komatsu Department of Molecular Genetics National Institute of Agrobiological SciencesTsukubaJapan

The technique of proteome analysis using 2-DE has the power tomonitor global changes that occur in the protein complement of tissuesand subcellular *** this review,we describe construction ofthe rice proteome database,the cataloging of rice proteins,and thefunctional characterization of some of the proteins ***,proteins extracted from various tissues and organelles were separated by

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Open Journal of Psychiatry 2014年第4期4卷 405-409页

作者： setsuko Taneichi Fumiharu Togo Tsukasa Sasaki Laboratory of Health Education Graduate School of Education Tokyo Japan

Aim: The aim of this study is to examine the effect of walking 10,000 steps per day on depressive symptoms for the company employees and their spouses in Japan. Method: Subjects were recruited from the participants of a walking campaign carried out by a Japanese company where the goal was to achieve 600,000 steps in 60 days. Among 221 subjects who participated in the campaign, 176 subjects (79.6%) agreed to participate in the present study. Sociodemographics and other information including depressive symptoms were assessed using a questionnaire. Result: Out of the 171 participants, 125 achieved the goal (73.1%). In the achiever’s group, the GHQ-12 score was significantly reduced at the end of the campaign compared with the non-achiever’s group. Exercise habit and the less overtime work (

关键词： Depressive Symptoms 10 000 Steps Physical Activity Walking Walking Campaign

The Yo-Yo tests and twelve-minute run test performance in young Japanese cross-country skiers

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Open Journal of Preventive Medicine 2013年第2期3卷 246-250页

作者： Denda Yuki Oh Taewoong Nakajima setsuko Graduate School of Health Science Matsumoto University Matsumoto Japan

Purpose: The aim of the present study is to examine the performance of young Japanese cross-country skiers of different competitive levels with the use of Yo-Yo tests and the 12-minute run test (12MRT) that have been demonstrated to be related to each level, and to know which test is suitable for different competitive performance. Methods: Elite group (N = 9) was 16.9 ± 0.3 years and moderate trained group (N = 22) was 16.1 ± 0.9 years. The testing sessions consisted of the following: 1) Vertical jump and 50m sprint;2) the Yo-Yo tests level 1 and 2 (intermittent recovery);3) 12MRT. Results: Results showed the vertical jump heights and 50m sprint that no significant both performances differences were found among the two groups. Significant the Yo-Yo tests and 12MRT performances differences were found between the two groups (p < 0.05). Performance test (%) showed the ratio of moderate trained group towards elite group. The Yo-Yo tests performance was low enough compared with the result of 12MRT in moderate trained group. Conclusions: It is therefore valid to assume that low Yo-Yo tests performance due to immature anaerobic in cross-country skiers was a new limiting factor in the competitive level.

关键词： Yo-Yo Intermittent Recovery Tests Endurance Sports Aerobic Anaerobic Performance

Usefulness of noninvasive transient elastography for assessment of liver fibrosis stage in chronic hepatitis C

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World Journal of Gastroenterology 2006年第48期12卷 7768-7773页

作者： Tadashi Takeda Takahiro Yasuda Yuji Nakayama Mika Nakaya Megumi Kimura Mariko Yamashita Ayumi Sawada Koji Abo setsuko Takeda Hiroki Sakaguchi Susumu Shiomi Hitoshi Asai Shuichi Seki Department of Hepatology Graduate School of Medicine Osaka City University Osaka Japan Central Clinical Laboratory Osaka City University Medical School Hospital Osaka Japan Department of Nuclear Medicine Graduate School of Medicine Osaka City University Osaka Japan Hitoshi Asai Health Service Center Osaka Kyoiku University Osaka Japan

AIM：To evaluate the method of noninvasive transient elastography for assessment of histological stage of liver fibrosis in patients with chronic hepatitis C （CHC）.METHODS： Two hundred and thirty-seven patients with CHC were included in this study. Liver biopsy was performed under ultrasonography on 217 of the patients, excluding twenty with clear clinical evidence of liver cirrhosis. Fifty subjects without liver disease were enrolled as a control group （stage 0）. Twentyfive patients with sustained virological response （SVR） to interferon （IFN） therapy were also enrolled. These patients underwent liver biopsy before IFN therapy. Examination of liver stiffness （LS） was performed by elastography.RESISTS： Medians （50% levels） of LS were 4.1 （3.5-4.9）, 6.3 （4.8-8.5）, 8.8 （6.8-12.0）, 14.6 （10.5-18.6）, and 22.2 （15.4-28.0）, respectively, in the fibrosis stages 0-4 （P 〈 0.001）. LS was significantly correlated with four serum fibrosis markers. LS values in patients with SVR were 3.8 （3.5-5.6）, 5.2 （4.4-6.8）, 6.8 （6.1-7.6）, and 6.1 （3.6-7.9）, respectively, in the fibrosis stages 1-4. In all stages, LS for patients with SVR was significantly lower than that for patients who did not undergo IFN therapy. LS was significantly correlated with serum concentrations of hyaluronic acid, type Ⅳ collagen, type Ⅳ collagen 7S, and type Ⅲ procollagen N peptide.CONCLUSION： LS correlated well with the histological stage of fibrosis. Changes in liver fibrosis stage may thus be estimated noninvasively using transient elastography.

关键词： Hepatitis C virus Echography Fibrosis Stiffness Interferon