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Seed-Specific Expression of Apolipoprotein A-IMilano Dimer in Engineered Rice Lines

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Rice science 2023年第6期30卷 587-597页

作者： serena REGGI Elisabetta ONELLI Alessandra MOSCATELLI Nadia STROPPA Matteo DELL’ANNO Kiril PERFANOV Luciana ROSSI Department of Veterinary Medicine and Animal Science University of MilanLodi 26900Italy Department of Biosciences University of MilanMilan 20133Italy Plantechno Srl Casalmaggiore 26040Italy

Apolipoprotein A-IMilano(ApoA-IM)has been shown to significantly reduce coronary atherosclerotic ***,the preparation of cost-effective pharmaceutical formulations of ApoA-IM is limited by the high cost and difficulty of purifying the protein and producing the highly effective dimeric *** aim of this study was to create an expression cassette that specifically drives the expression of dimeric ApoA-IM in the protein bodies of rice *** ApoA-IM protein under control of the 13 kDa prolamin promoter is expressed exclusively in its dimeric form within the seeds,and immunocytochemical and immunogold analyses confirmed its expression in different caryopsis tissue such as seed coat,aleurone cell and endosperm,particularly in amyloplast and storage vacuoles.A plant-based ApoA-IM production system offered numerous advantages over current production systems,including the direct production of the most therapeutically effective dimeric ApoA-IM forms,long-term protein storage in seeds,and ease of protein production by simply growing ***,seeds had the potential to serve as a costeffective source of therapeutic ApoA-IM.

关键词： apolipoprotein A-IMilano engineered plant immunofluorescence immunogold analysis rice seed-specific promoter

Adjusting the physico-chemical properties of collagen scaffolds to accommodate primary osteoblasts and endothelial cells

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Regenerative Biomaterials 2023年第1期10卷 590-602页

作者： Nima Meyer Daniel V.Bax Jochen Beck Ruth E.Cameron serena M.Best Department of Materials Science and Metallurgy University of CambridgeCambridge CB30FSUK Geistlich Pharma AG Product DevelopmentWolhusenSwitzerland

Collagen-based biomaterials are used widely as tissue engineering scaffolds because of their excellent bioactivity and their similarity to the natural *** regeneration of healthy bone tissue requires simultaneous support for both osteoblasts and,where angiogenesis is intended,endothelial *** it is important to tailor carefully the biochemical and structural characteristics of the scaffold to suit the needs of each cell *** work describes for the first time a systematic study to gain insight into the cell type-specific response of primary human osteoblast(hOBs)and human dermal microvascular endothelial cells(HDMECs)to insoluble collagen-based *** behaviour was evaluated on both 2D films and 3D scaffolds,produced using *** collagen was cross-linked at various EDC/NHS concentrations and mono-cultured with hOBs and HDMECs to assess the effect of architectural features and scaffold stabilization on cell *** was observed that 3D scaffolds cross-linked at 30%of the standard conditions in literature offered an optimal combination of mechanical stiffness and cellular response for both cell types,although endothelial cells were more sensitive to the degree of cross-linking than *** features have a time-dependent impact on the cell migration profile,with alignment being the most influential parameter overall.

关键词： angiogenesis collagen scaffolds cell migration EDC/NHS cross-linking

High risk of temporomandibular disorder in irritable bowel syndrome: Is there a correlation with greater illness severity?

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World Journal of Gastroenterology 2017年第1期23卷 103-109页

作者： serena Gallotta Vincenzo Bruno Santo Catapano Nicola Mobilio Carolina Ciacci Paola Iovino Functional GI Disorders Center at Gastrointestinal Unit AOU S. Giovanni di Dio e Ruggi d’Aragona Department of Medicine and Surgery University of Salerno Dental School University of Ferrara

AIMTo investigate the prevalence and the risk of temporomandibular disorders (TMDs) in patients with irritable bowel syndrome (IBS) (including each subtype: constipation, diarrhoea, and mixed) compared to the general *** January 2014 and December 2015 we enrolled consecutively adult patients diagnosed with IBS at the outpatient clinic of the University of Salerno and healthy controls (HC) without IBS. At enrollment, we analyzed all patients for the presence of TMDs according to the Research Diagnostic Criteria for *** enrolled 91 IBS patients (23 IBS-D, 30 IBS-C and 38 IBS-M) and 57 HC in the study. We found a higher risk of having TMD (OR = 3.41, 95%CI: 1.66-7.01) compared to the HC. The risk of having TMD was independent of IBS-subtype. Multiple regression analysis showed that facial pain was positively related to abdominal pain and higher level of *** patients had a more than three times greater risk of TMD compared to HC. The risk of having TMD was similar in different IBS subtypes. IBS patients that also fulfilled criteria for TMD seem to share along with chronic facial and abdominal pain a significant co-occurrence with psychiatric disorders and female preponderance.

关键词： Temporomandibular disorders Irritable bowel syndrome Chronic pain Facial pain Abdominal pain Irritable bowel syndrome severity score symptoms Irritable bowel syndrome predominant diarrhea Irritable bowel syndrome predominant constipation Irritable bowel syndrome mixed

Glacial biodiversity of the southernmost glaciers of the European Alps(Clapier and Peirabroc,Italy)

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Journal of Mountain Science 2022年第8期19卷 2139-2159页

作者： Barbara VALLE Mauro GOBBI Marta TOGNETTI Marina serena BORGATTI Chiara COMPOSTELLA Paolo PANTINI Marco CACCIANIGA Universitàdegli Studi di Milano Department of BiosciencesVia Celoria 2620133 MilanoItaly MUSE-Science Museum of Trento Research and Museum Collections OfficeClimate and Ecology UnitCorso del Lavoro e della Scienza338122 TrentoItaly Universitàdegli Studi di Milano Dipartimento di Scienze della Terra"A.Desio"Via Mangiagalli 3420133 MilanoItaly Museo Civico di Scienze Naturali"E.Caffi" Piazza della Cittadella1024129 BergamoItaly

We applied a multi-taxa approach integrating the co-occurrence of plants,ground beetles,spiders and springtails with soil parameters(temperatures and chemical characteristics)in order to describe the primary succession along two glacier forelands in the Maritime Alps(Italy),a hotspot of Mediterranean *** compared these successions to those from Central Alps:Maritime glacier forelands markedly differ for their higher values of species richness and species *** to our expectation,Maritime glacier forelands follow a‘replacement change model’,like continental succession of Inner Alps and differently from other peripheral *** propose that the temperatures along these Mediterranean glacier forelands are warmer than those along other Alpine glacier forelands,which promote the faster species ***,we found that early and mid successional stages of the investigated glaciers are richer in cold-adapted and endemic species than the later ones:we confirmed that the‘replacement change’model disadvantages pioneer,cold-adapted *** the overall correspondence among coldadapted and endemic species,the most threatened in this climate phase,our results raise new concerns about the extinction risk of these *** also describe supraglacial habitat of Maritime glaciers demonstrating that supraglacial debris represents an environment decoupled from the regional climate and may have an important role as refugium for coldadapted and hygrophilous plant and animal species,whose survival can be threatened by climate change and by a rapid ecological succession in the adjacent forelands.

关键词： Arthropod communities Cold-adapted species Glacier forelands Plant communities Primary succession

New endoscopy advances to refine adenoma detection rate for colorectal cancer screening:None is the winner

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World Journal of Gastrointestinal Oncology 2017年第10期9卷 402-406页

作者： Marcello Maida Salvatore Camilleri Michele Manganaro serena Garufi Giuseppe Scarpulla Section of Gastroenterology S.Elia-M.Raimondi Hospital93100 CaltanissettaItaly

Colorectal cancer(CRC) is the third most common cancer in males and second in females, and globally the fourth cause for cancer death worldwide. Oncological screening of CRC has a major role in the management of the disease and it is mostly performed by colonoscopy. Anyway, effectiveness of endoscopic screening for CRC strictly depends on adequate detection and removal of potentially precancerous lesions, and accuracy of colonoscopy in detection of adenomas is still suboptimal. For this reason, several technological advances have been implemented in order to improve the diagnostic sensitivity of colonoscopy in adenoma detection. Among these:(1) Visual technologies such as chromoendoscopy and narrow band imaging;(2) optical innovation as high definition endoscopy, full-spectrum endoscopy or Third Eye Retroscope; and(3) mechanical advances as Cap assisted colonoscopy, Endocuff, Endoring and G-Eye endoscope. All these technologies advances have been tested over time by clinical studies with mixed results. Which of them is more likely to be successful in the next future?

关键词： Colorectal cancer screening Colonoscopy Adenoma detection rate Diagnostic advances

Indications,limitations and maneuvers to enable extended hepatectomy:Current trends

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World Journal of Gastroenterology 2014年第24期20卷 7887-7893页

作者： Dimitrios Dimitroulis Petros Tsaparas serena Valsami Dimitrios Mantas Eleftherios Spartalis Charalampos Markakis Gregory Kouraklis Second Department of Propedeutic Surgery Athens University Medical School"Laiko" Hospital11527 AthensGreece Blood Transfusion Department Athens University Medical SchoolAreteion Hospital11527 AthensGreece

The liver is a solid organ with a wide variety of primary benign or malignant tumors as well as metastatic *** resection of these tumors remains the only curative *** limitations,however,do not allow the performance of these *** review evaluates the indications and limitations regarding these extended hepatic resections,as well as describing all the manipulations that increase the candidates for such operations.A thorough review of the literature was performed in order to define indications for extended hepatectomy,as well as to present all methods that contribute to increasing the volume of the future remnant *** role of portal vein ligation,portal vein embolization,two-stage hepatectomy,and in situ liver transection are evaluated in the setting of indications and *** hepatectomies are a necessity due to oncological *** methods developed in order to increase the volume of the remnant liver are safe and *** situ liver transection is a novel and revolutionary two-step procedure for extended hepatic *** clinical studies are required to estimate long-term results and the oncological basis of this technique.

关键词： Extended hepatectomy Portal vein ligation Remnant liver volume

Gastrointestinal and hepatic involvement during COVID-19 pandemic: A focus on pediatric population and possible future implications

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World Journal of Gastroenterology 2021年第40期27卷 7000-7004页

作者： Rossella Sica serena Pennoni Laura Penta Sara Riccioni Giuseppe Di Cara Alberto Verrotti Department of Pediatrics University of PerugiaPerugia 06132PGItaly

Since the coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has rapidly spread worldwide,there is still limited knowledge about this condition and its natural *** have been relatively spared during COVID-19 pandemic but a novel syndrome known as multisystem inflammatory syndrome(MIS-C)has emerged,following a SARS-CoV-2 infection in children and *** syndrome can lead to shock and multiple organ failure requiring intensive *** COVID-19 clinical research focuses on respiratory symptoms,extrapulmonary involvement such as gastrointestinal(GI)and hepatic manifestations should also be *** fact,GI and hepatic involvement play an important role among the most common presenting symptoms of both pediatric and adult COVID-19 and *** involvement can not only be one of the most common presenting clinical features but also one of the sequelae of these *** ultrasonography monitoring could be very useful to identify a potential involvement of the GI tract and ***,long-term follow-up is needed and would be essential to define the long-term outcomes of these patients.

关键词： SARS-CoV-2 COVID-19 Multisystem inflammatory syndrome Gastrointestinal tract Liver Ultrasonography

The Importance of Being Poirot： A Critical Examination of Curtain as Seen on ITV

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Journalism and Mass Communication 2015年第3期5卷 112-118页

作者： serena Formica The University of Derby Leeds UK

Hercule Poirot has died, and his death was witnessed by the nearly five million viewers ofCurtain： Poirot＇s last case, the concluding episode of the long lasting ITV television series Agatha Christie＇s Poirot. Poirot was played for one final time by David Suchet, who, in the course of the series, has managed to appropriate the character to an extent that no previous actor has achieved. This article suggests that Suchet has indeed made of Poirot a ＂fictional celebrity＂, and analyses Curtain on the one hand by looking at its critical reception, and on the other by considering the hour-long documentary Being Poirot, in which David Suchet discusses his personal journey as the character.

关键词： Agatha Christie Poirot David Suchet

Fools in Ludwig Tieck’s Der gestiefelte Kater

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Journal of Literature and Art Studies 2021年第8期11卷 601-607页

作者： serena Spazzarini University of Genoa GenoaItaly

The aim of this essay is to consider how Tieck uses the Narrheit concept in his work Der gestiefelte Kater as a key to *** doing so,it is also possible to grasp the semantic variability and the multiple layering of the Narr *** calls some of the heroes of his comedy Narr,and through these Narren-figures intended to describe some weak points of the society of his time-he intends to reflect on the correctness of the order in late eighteenth-century society.

关键词： Der gestiefelte Kater fool Narrheit Sebastian Brant