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C2 domain of synaptotagmin I associates with lipid rafts of plasma membrane

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Chinese Science Bulletin 2008年第9期53卷 1373-1380页

作者： LU JiHua HE Li SUI senfang Department of Biological Sciences & Biotechnology State Key Laboratory of Biomembrane and Membrane Biotechnology TsinghuaUniversity Beijing 100084 China

In this paper we report that the C2 domain of synaptotagmin I (syt I) could associate with lipid rafts of plasma membrane. We demonstrate that phosphatidylinositol 4,5-bisphosphate (PIP2) in the target membrane and Ca2+ are the key factors to enhance the raft association of the C2 domain. We also found that the raft association of the C2 domain could be fulfilled by either C2A or C2B alone, suggesting that their raft association might be complementary. Finally, we indicate that destroying lipid rafts or blocking syt I-raft association could significantly reduce the Ca2+-driven release of glutamates. Our data indicate that the raft association of the C2 domain might play an important role in the regulated exocytosis.

关键词：碳晶体脂质等离子体膜薄

Two-dimensional crystallization of photosystem I chlorophyll a/b protein complex from cucumber

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Chinese Science Bulletin 1997年第7期42卷 597-601页

作者： LIU Zheng SUI senfang WANG Shaoxiong LOU Shiqing WANG Kefen ZHAO Fuhong KUANG Tingyun Department of Biological Sciences and Biotechnology Tsinghua Univcrsity Beijing China Institute of Botany Chinese Academy of Sciences Beijing China

THE photosystem Ⅱ light-harvesting chlorophyll a/b protein complex (LHC Ⅱ) is a kind of integral membrane protein complex in the thylakoid membranes of chloroplasts from higher plants and green algae, and its most i... 详细信息

关键词： photosystem n light-harvesting chlorophyll a/b protein complex (LHC Ⅱ) two-dimensional crystallization electron crystallography biological diversity.

Regulatory dynamics of the higher-plant PSI-LHCI supercomplex during state transitions

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Molecular Plant 2023年第12期16卷 1937-1950页

作者： Jianghao Wu Shuaijiabin Chen Chao Wang Weijun Lin Chao Huang Chengxu Fan Dexian Han Dandan Lu Xiumei Xu senfang Sui Lixin Zhang State Key Laboratory of Crop Stress Adaptation and Improvement School of Life SciencesHenan UniversityJinming AvenueKaifeng 475004China School of Life Science Southern University of Science and TechnologyShenzhen 518055China State Key Laboratory of Membrane Biology Beijing Advanced Innovation Center for Structural BiologyBeijing Frontier Research Center for Biological StructureSchool of Life SciencesTsinghua UniversityBeijing 100084China Cryo-EM Center Southern University of Science and TechnologyShenzhen 518055China Photosynthesis Research Center Key Laboratory of PhotobiologyInsttute of BotanyChinese Academy of SciencesBejing 100093China

State transition is a fundamental light acclimation mechanism of photosynthetic organisms in response to the environmental light *** process rebalances the excitation energy between photosystemI(PSl)and photosystem Il through regulated reversible binding of the light-harvesting complex Il(LHCll)to ***,the structural reorganization of PSI-LHCI,the dynamic binding of LHCll,and the regulatory mechanisms underlying state transitions are less understood in higher *** this study,using cryoelectron microscopy we resolved the structures of PSI-LHCI in both state 1(PSI-LHCI-ST1)and state 2(PSILHCI-LHCll-ST2)from Arabidopsis *** genetic and functional analyses revealed novel contacts between Lhcb1 and PsaK that further enhanced the binding of the LHCll trimer to the PSI core with the known interactions between phosphorylated Lhcb2 and the PsaL/PsaH/PsaO ***,PsaO was absent in the PSI-LHCI-ST1 supercomplex but present in the PSI-LHCI-LHCIl-ST2 supercomplex,in which the PsaL/PsaK/PsaA subunits undergo several conformational changes to strengthen the binding of PsaO in ***,the PSI-LHCI module adopts a more compact configuration with shorter Mg-to-Mg distances between the chlorophylls,which may enhance the energy transfer efficiency from the peripheral antenna to the PSl core in ***,our work provides novel structural and functional insights into the mechanisms of light acclimation during state transitions in higher plants.

关键词： state transitions dynamic conformation photosystem l regulatory mechanism Arabidopsis thaliana

Two forms of the membrane-bound state of the first C2 domain (C2A) of synaptotagminⅠand calcium-triggered membrane insertion

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Chinese Science Bulletin 2003年第17期48卷 1822-1827页

作者： HE Yuhong, LI Xianghui, WANG Fu, XUE Yi & SUI senfang Department of Biological Sciences & Biotechnology, State Key Labora-tory of Biomembrane, Tsinghua University, Beijing 100084, China Department of Biological Sciences and Biotechnology State Key Laboratory of Biomembrane Tsinghua University Beijing 100084 China

The synaptic vesicle protein synaptotagminⅠ (sytⅠ) is a vesicle transmembrane protein present in synap-tic vesicles, which has been proposed as the Ca2+ sensor that regulates secretion. The C2A domain is the membrane proximal part of its cytoplasmic domain. The interaction between C2A and lipid bilayer has been considered to be essential for triggering neurotransmitter release. In the pre-sent work, the measurements of membrane surface tension and surface concentration showed that the C2A domain of sytⅠexhibited two membrane-bound states: the surface adsorption state and the membrane insertion state. The sur-face absorption state formed in a Ca2+-independent manner with lower affinity, while the membrane insertion state formed with high affinity was only found in the presence of Ca2+. Both the Ca2+-independent and Ca2+-dependent sytⅠ- membrane interactions required anionic phospholipids, such as phosphatidylserine (PS). When expressed into rat pheo-chromocytoma (PC12) cells and human embryonic kidney (HEK-293) cells, as demonstrated by immunofluorescence staining and subcellular fractionation, most of the C2A was found at the plasma membrane, even when the cells were depleted of Ca2+ by incubation with EGTA. These results suggested a new molecular mechanism of sytⅠas a Ca2+ sensor in membrane fusion. Ca2+-independent surface ad-sorption might attach sytⅠto the release site during the docking or priming step. When intracellular Ca2+ increased, sytⅠtriggered the neurotransmitter release following the Ca2+-dependent penetration into the target membrane.

关键词：突触小泡蛋白质膜贯通蛋白质钙离子薄膜插入 C2A 薄膜边界状态

Effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of bone marrow stromal cells and on the adipogenic trans-differentiation of osteoblasts

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Progress in Natural Science:Materials International 2005年第11期15卷 993-999页

作者： LI Xianghui, ZHANG Jinchao, SUI senfang and YANG Mengsu (State Key Laboratory of Biomembrane, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China Department of Chemistry, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China) State Key Laboratory of Biomembrane Department of Biological Sciences and Biotechnology Tsinghua University Beijing 100084 China Department of Chemistry College of Chemistry and Environmental Science Hebei University Baoding 071002 China Department of Biology and Chemistry City University of Hong Kong Hong Kong Hong Kong

The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), alkaline phosphatase (ALP) activity and oil red O assays were used to examine the effects of genistein, daidzein and glycitein on the osteogenic and adipogenic differentiation of primary mouse bone marrow stromal cells (MSCs) and the adipogenic trans-differentiation of primary mouse osteoblasts. The results indicated that daidzein, genistein and glycitein at concentrations from 1×10-8 mol/L to 1×10-5mol/L promoted the proliferation of MSCs and osteoblasts; genistein, daidzein and glycitein promoted osteogenic differentiation and inhibited adipogenic differentiation of MSCs, and inhibited adipocytic transdifferentiation of osteoblasts at appropriate concentrations as 17β-estradiol. It suggests that genistein, daidzein and glycitein regulate a dual differentiational process of MSCs into the osteogenic and adipogenic lineages, and trans-differentiational process of primary osteoblasts into the adipocyte lineages, causing a lineage shift toward osteoblast. Protective effects of them on bone may be mediated by a reversal of adipogenesis which may promote the proliferation, differentiation and mineralization of osteoblasts, and make adipocytes secrete less cytokines which may promote osteoclast formation and activation. In addition, the results also indicated that genistein, daidzein and glycitein may be helpful in preventing the development of steroid induced osteonecrosis.

关键词： genistein daidzein glycitein bone marrow stromal cells osteoblasts osteogenic differentiation adipogenic differentiation adipogenic trans-differentiation.

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镁离子转运蛋白CorA间质结构域删除分析

清华大学学报（自然科学版） 2008年第6期48卷 1057-1061页

作者：孙占华王世珍黄萱许淼隋森芳清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室北京100084

为了研究CorA的镁离子转运机理,对大肠杆菌CorA的间质结构域区进行了删除突变分析,并利用酵母突变体互补技术进行了突变体活性测定。以酿酒酵母质膜镁离子转运系统敲除突变体菌株CM66及其基因型对照菌株CM52为宿主菌,建立了大肠杆菌Cor... 详细信息

为了研究CorA的镁离子转运机理,对大肠杆菌CorA的间质结构域区进行了删除突变分析,并利用酵母突变体互补技术进行了突变体活性测定。以酿酒酵母质膜镁离子转运系统敲除突变体菌株CM66及其基因型对照菌株CM52为宿主菌,建立了大肠杆菌CorA转运活性测定体系。结果显示:大肠杆菌CorA间质结构域N-端起始的24个残基对于CorA在酵母质膜中的表达或维持其本身正确构象发挥重要作用,CorA M124到D154段的部分残基在CorA介导的镁离子转运过程中起到重要作用。

关键词：分子生物物理学镁离子转运系统钴离子抗性等位基因(CorA) 间质结构域删除分析

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福建周宁芹溪银多金属矿地质特征及深部找矿

福建地质 2016年第2期35卷 125-134页

作者：钟森芳福建省第四地质大队宁德352100

周宁芹溪银多金属矿床产于南园组第三段流纹质晶玻屑凝灰岩中，矿体呈脉状、透镜状产出。矿物成分以银（金）矿为主，具水平、垂直分带现象。其成因为浅成中低温火山一次火山热液银多金属矿床。根据深部找矿理论及相关矿体的产状、形态... 详细信息

周宁芹溪银多金属矿床产于南园组第三段流纹质晶玻屑凝灰岩中，矿体呈脉状、透镜状产出。矿物成分以银（金）矿为主，具水平、垂直分带现象。其成因为浅成中低温火山一次火山热液银多金属矿床。根据深部找矿理论及相关矿体的产状、形态及控矿特征，提出955～850m中段可作为新的找矿地段，通过地质勘探，已求得955～850m中段新增（122b＋333）矿石资源储量72．35万t。

关键词：银多金属矿矿床特征深部找矿周宁芹溪

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计算机信息安全管理存在的问题及对策

信息与电脑 2020年第11期32卷 229-230页

作者：郭森芳中国人民财产保险股份有限公司湖州市分公司浙江湖州313000

安全管理是计算机程序开发与应用的主要内容,与计算机虚拟平台的运营成效之间有密切的关系。为此,笔者结合计算机信息安全管理的相关理论,从外部防护、内部管理等方面,对计算机信息安全管理的问题与对策进行分析,以达到明晰技术要点、... 详细信息

安全管理是计算机程序开发与应用的主要内容,与计算机虚拟平台的运营成效之间有密切的关系。为此,笔者结合计算机信息安全管理的相关理论,从外部防护、内部管理等方面,对计算机信息安全管理的问题与对策进行分析,以达到明晰技术要点、促进我国信息化服务体系建设的目的。

关键词：计算机信息安全管理信息化

少数民族地区语文翻译事业的发展对策研究--以湖南怀化民族地区为例

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文化创新比较研究 2021年第18期5卷 137-140页

作者：左森方怀化学院湖南怀化418000

民族地区语言文化翻译事业的发展关系到民族地区的稳定团结和全面发展,该文通过分析怀化地区民族语文翻译事业,分析发展民族语文翻译事业的长远意义和现实意义,探讨民族语文翻译事业的发展对策,认为只有提高认识,科学规划,统筹兼顾,加... 详细信息

民族地区语言文化翻译事业的发展关系到民族地区的稳定团结和全面发展,该文通过分析怀化地区民族语文翻译事业,分析发展民族语文翻译事业的长远意义和现实意义,探讨民族语文翻译事业的发展对策,认为只有提高认识,科学规划,统筹兼顾,加强部门和行业建设,深化理论研究和实践应用,才能切实发展好民族地区语文的翻译事业。

关键词：湖南怀化民族语文翻译发展问题发展对策