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Ascitic Fluid Analysis in the Differential Diagnosis of Ascites:Focus on Cirrhotic Ascites

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Journal of Clinical and Translational Hepatology 2014年第1期2卷 58-64页

作者： lin-lin Huang Harry Hua-Xiang Xia sen-lin zhu Department of Gastroenterology the First Affiliated Hospital Sun Yat-sen University Guangzhou China Novartis Pharmaceuticals Corporation East Hanover NJ USA

Ascites is the pathologic accumulation of fluid within the peritoneal *** many diseases can cause ascites,in particular cirrhosis,samples of ascitic fluid are commonly analyzed in order to develop a differential *** concept of transudate versus exudate,as determined by total protein measurements,is outdated and the use of serumascites albumin gradient as an indicator of portal hypertension is more *** dehydrogenase (LDH),vascular endothelial growth factor (VEGF),and other tumor markers can be helpful in distinguishing between malignant and benign *** and adenosine deaminase levels may support a diagnosis of tuberculous disease,and amylase level may indicate a diagnosis of *** the specificity and sensitivity of laboratory results,accurate diagnosis should be based on both laboratory data and clinical iudgment.

关键词： Ascitic fluid analysis Differential diagnosis Ascites Cirrhosis

A flexible electrode array for determining regions of motor function activated by epidural spinal cord stimulation in rats with spinal cord injury

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Neural Regeneration Research 2022年第3期17卷 601-607页

作者： Guang-Wei Mao Jian-Jun Zhang Hao Su Zhi-Jun Zhou lin-sen zhu Xiao-Ying Lü Zhi-Gong Wang State Key Laboratory of Bioelectronics Southeast UniversityNanjingJiangsu ProvinceChina Institute of RF-&OE-ICs Southeast UniversityNanjingJiangsu ProvinceChina Co-innovation Center of Neuroregeneration Nantong UniversityNantongJiangsu ProvinceChina

Epidural stimulation of the spinal cord is a promising technique for the recovery of motor function after spinal cord *** key challenges within the reconstruction of motor function for paralyzed limbs are the precise control of sites and parameters of *** activate lower-limb muscles precisely by epidural spinal cord stimulation,we proposed a high-density,flexible electrode *** determined the regions of motor function that were activated upon epidural stimulation of the spinal cord in a rat model with complete spinal cord,which was established by a transection *** evaluating the effect of stimulation,the evoked potentials were recorded from bilateral lowerlimb muscles,including the vastus lateralis,semitendinosus,tibialis anterior,and medial *** determine the appropriate stimulation sites and parameters of the lower muscles,the stimulation characteristics were studied within the regions in which motor function was activated upon spinal cord *** the vastus lateralis and medial gastrocnemius,these regions were symmetrically located at the lateral site of L1 and the medial site of L2 vertebrae segment,*** tibialis anterior and semitendinosus only responded to stimulation simultaneously with other *** minimum and maximum stimulation threshold currents of the vastus lateralis were higher than those of the medial *** results demonstrate the ability to identify specific stimulation sites of lower muscles using a high-density and flexible *** also provide a reference for selecting the appropriate conditions for implantable stimulation for animal models of spinal cord *** study was approved by the Animal Research Committee of Southeast University,China(approval No.20190720001) on July 20,2019.

关键词： electrode array epidural spinal cord stimulation evoked potentials motor function rehabilitation spinal cord injury spinal segment

Macrophage migration inhibitory factor as a potential prognostic factor in gastric cancer

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World Journal of Gastroenterology 2015年第34期21卷 9916-9926页

作者： Long-Jun He Dan Xie Pin-Jin Hu Yi-Ji Liao Hai-Xia Deng Hsiang-Fu Kung sen-lin zhu Department of Gastroenterology the First Affiliated HospitalSun Yat-sen University Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer Medicine Stanley Ho Center for Emerging Infectious Diseases and Li Ka Shing Institute of Health Sciences State Key Laboratory of Oncology in South China The Chinese university of Hong Kong

AIM:To investigate macrophage migration inhibitory factor(MIF) expression and its clinical relevance in gastric cancer,and effects of MIF knockdown on proliferation of gastric cancer cells. METHODS:Tissue microarray containing 117 samples of gastric cancer and adjacent non-cancer normal tissues was studied for MIF expression by immunohistochemistry(IHC) semiquantitatively,and the association of MIF expression with clinical parameters was analyzed. MIF expression in gastric cancer cell lines was detected by reverse transcriptionpolymerase chain reaction(RT-PCR) and Western blot. Two pairs of si RNA targeting the MIF gene(MIF si-1 and MIF si-2) and one pair of scrambled si RNA as a negative control(NC) were designed and chemically synthesized. All si RNAs were transiently transfected in AGS cells with OligofectamineTM to knock down the MIF expression,with the NC group and mock group(OligofectamineTM alone) as controls. At 24,48,and 72 h after transfection,MIF m RNA was analyzed by RTPCR,and MIF and proliferating cell nuclear antigen(PCNA) proteins were detected by Western *** proliferative rate of AGS cells was assessed by methylthiazolyl tetrazolium(MTT) assay and colony forming ***:The tissue microarray was informative for IHC staining,in which the MIF expression in gastric cancer tissues was higher than that in adjacent noncancer normal tissues(P ling times were assayed by MTT 4 d after transfection,and colonies formed were assayed by

关键词： Macrophage migration inhibitory factor Proliferati

DNA展示系统介导的断裂蛋白质内含子体外定向进化方法的研究

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中国生物化学与分子生物学报 2016年第10期32卷 1121-1131页

作者：林森珠陈格飞孟清东华大学生物科学与技术研究所上海201620 卡罗林斯卡学院老年痴呆研究中心瑞典斯德哥尔摩14157

蛋白质内含子对于外显子的选择性限制了它的应用范围。目前,已经建立的卡那霉素定向进化系统适用于微小蛋白质内含子,但不适用于断裂蛋白质内含子。为了研究适用于断裂蛋白质内含子的定向进化的方法,我们引入了DNA展示系统。在该系统中... 详细信息

蛋白质内含子对于外显子的选择性限制了它的应用范围。目前,已经建立的卡那霉素定向进化系统适用于微小蛋白质内含子,但不适用于断裂蛋白质内含子。为了研究适用于断裂蛋白质内含子的定向进化的方法,我们引入了DNA展示系统。在该系统中,生物素化基因在人工细胞中与其表达的融合蛋白(内含子C端-外显子-生物素结合蛋白)形成DNA-蛋白质连接体。只有能与后续加入的N端蛋白质内含子前体(Flag-内含子N端)发生剪接反应的DNA-蛋白质连接体,才能被加上旗帜标签(Flag),从而被抗旗帜标签抗体纯化柱(Anti Flag antibody M2 agarose)筛选富集。为了验证该系统的可行性,实验构建了2个基因,即含有具剪接活性的蛋白质内含子的阳性基因IRC和含有不具剪接活性的蛋白质内含子的阴性基因IRCM。实验首先通过Western印迹和琼脂糖凝胶电泳证明,人工细胞中的体外转录翻译系统不仅可高表达500个氨基酸的蛋白质,而且表达的蛋白中内含子仍保持原有的剪接能力。生物素结合蛋白能结合95%的DNA,并且形成的DNA-蛋白质连接体可以被筛选富集,最终证明了该系统用于断裂蛋白质内含子定向进化筛选的可行性。随后,为了检测系统的富集效率,制备了人为的基因"突变库",即将基因IRC和IRCM以摩尔比1:10的比例混合,经过2轮筛选后,阳性基因IRC可以被10倍富集,进一步证明了体外筛选方法的可行性。该方法为后续针对不同宿主进化出不同的断裂蛋白质内含子提供筛选方法支持,也为断裂蛋白质内含子的在生物技术和研究领域的应用奠定了基础。

关键词：断裂蛋白质内含子体外定向进化 DNA展示系统人工细胞体外转录翻译系统

Non-coding RNAs in acute ischemic stroke:from brain to periphery

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Neural Regeneration Research 2025年第1期20卷 116-129页

作者： Shuo Li Zhaohan Xu Shiyao Zhang Huiling Sun Xiaodan Qin lin zhu Teng Jiang Junshan Zhou Fuling Yan Qiwen Deng Department of Neurology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina Department of Neurology Zhongda HospitalSchool of MedicineSoutheast UniversityNanjingJiangsu ProvinceChina General Clinical Research Center Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu ProvinceChina

Acute ischemic stroke is a clinical emergency and a condition with high morbidity,mortality,and *** predictive,diagnostic,and prognostic biomarkers and effective therapeutic targets for acute ischemic stroke remain *** innovations in high-throughput gene sequencing analysis,many aberrantly expressed non-coding RNAs(ncRNAs)in the brain and peripheral blood after acute ischemic stroke have been found in clinical samples and experimental *** expressed ncRNAs in the post-stroke brain were demonstrated to play vital roles in pathological processes,leading to neuroprotection or deterioration,thus ncRNAs can serve as therapeutic targets in acute ischemic ***,distinctly expressed ncRNAs in the peripheral blood can be used as biomarkers for acute ischemic stroke prediction,diagnosis,and *** particular,ncRNAs in peripheral immune cells were recently shown to be involved in the peripheral and brain immune response after acute ischemic *** this review,we consolidate the latest progress of research into the roles of ncRNAs(microRNAs,long ncRNAs,and circular RNAs)in the pathological processes of acute ischemic stroke–induced brain damage,as well as the potential of these ncRNAs to act as biomarkers for acute ischemic stroke prediction,diagnosis,and *** from this review will provide novel ideas for the clinical application of ncRNAs in acute ischemic stroke.

关键词： acute ischemic stroke apoptosis blood–brain barrier damage circular RNAs excitatory toxicity long non-coding RNAs microRNAs neuroinflammation non-coding RNAs oxidative stress

幽门螺杆菌疫苗接种小鼠产生免疫后胃炎的影响因素

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世界华人消化杂志 2006年第23期14卷 2275-2280页

作者：陈洁陈旻湖王锦辉朱森林中山大学附属第一医院消化内科广东省广州市510080

目的:研究幽门螺杆菌(H pylorl)疫苗接种小鼠产生免疫后胃炎的影响因素．方法:将H pylori疫苗免疫C57BL／6和BALB／c的小鼠,观察攻击后胃黏膜H pylori定植和炎症情况．将H pylori疫苗免疫C57BL／6小鼠,然后予不同菌量的H pylori攻击,观... 详细信息

目的:研究幽门螺杆菌(H pylorl)疫苗接种小鼠产生免疫后胃炎的影响因素．方法:将H pylori疫苗免疫C57BL／6和BALB／c的小鼠,观察攻击后胃黏膜H pylori定植和炎症情况．将H pylori疫苗免疫C57BL／6小鼠,然后予不同菌量的H pylori攻击,观察胃黏膜H Pylori定植和炎症情况．将H pylori疫苗经口和经腹腔免疫C57BL／6小鼠,观察攻击后胃黏膜H pylori定植和炎症情况.对感染H pylori的C57BL／6小鼠予H pylori疫苗治疗,观察治疗免疫后胃黏膜H pylori定植和炎症情况．结果:不同品系的小鼠免疫保护程度无明显差异,但C57BL／6小鼠免疫后胃炎重于BALB／c小鼠．接受不同攻击茵量的小鼠保护程度无明显差异,但大的攻击茵量可诱导更严重的免疫后炎症．不同免疫途径诱导的免疫保护程度及攻击后不同时间点的炎症程度均无显著性差异．治疗性免疫导致H pylori定植明显降低,同时也引发更为严重的胃炎．结论:在不同的免疫宿主、免疫途径和治疗性免疫中均存在免疫后胃炎．免疫后胃炎的强弱程度受免疫宿主和攻击菌量的影响．

关键词：幽门螺杆菌疫苗免疫后胃炎小鼠

巨噬细胞游走抑制因子在卵巢癌侵袭中的作用与意义

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癌症 2009年第10期28卷 1054-1060页

作者：武鸿美朱森林贺龙君刘艳辉谢丹华南肿瘤学国家重点实验室广东广州510060 中山大学肿瘤防治中心实验研究部广东广州510060 中山大学附属第一医院内科广东广州510080 广东省人民医院病理科广东广州510080

背景与目的:巨噬细胞游走抑制因子(macrophage migration inhibitory factor,MIF)与肿瘤的恶性进展密切相关。本研究探讨MIF基因对卵巢癌HO-8910和OVCAR-3细胞侵袭和增殖能力的影响及其在卵巢癌组织中表达的意义。方法:瞬时转染小干扰RN... 详细信息

背景与目的:巨噬细胞游走抑制因子(macrophage migration inhibitory factor,MIF)与肿瘤的恶性进展密切相关。本研究探讨MIF基因对卵巢癌HO-8910和OVCAR-3细胞侵袭和增殖能力的影响及其在卵巢癌组织中表达的意义。方法:瞬时转染小干扰RNA(small interferingRNA,siRNA)靶向敲除MIF基因,RT-PCR和Westernblot检测MIF在mRNA和蛋白水平的表达,通过体外迁移、侵袭实验和噻唑蓝比色法(MTT)分析MIF对HO-8910和OVCAR-3细胞迁移、侵袭和增殖能力的影响;免疫组织化学检测MIF蛋白在卵巢癌组织中的表达情况。结果:与阴性对照组细胞比较,瞬时转染MIFsiRNA的HO-8910和OVCAR-3细胞中MIF基因表达水平明显降低。MTT结果显示,转染MIFsiRNA的两株卵巢癌细胞增殖率显著低于阴性对照组(P<0.05)。转染MIFsiRNA的HO-8910细胞穿膜细胞数(MIF-si1:48.0±7.3;MIF-si2:38.0±3.6)与阴性对照组(78.0±8.5)比较差异有统计学意义(P<0.05),其穿过铺了Matrigel膜的细胞数(MIF-si1:35.0±5.0;MIF-si2:30.0±5.6)也显著少于阴性对照组(P<0.05)。同样,转染了MIFsiRNA的OVCAR-3细胞穿膜细胞数(MIF-si1:40.0±4.5;MIF-si2:42.0±3.0)与阴性对照组(65±2.1)比较,差异也有统计学意义(P<0.05),其穿过铺了Matrigel膜的细胞数(MIF-si1:25.0±3.0;MIF-si2:27.0±3.4)也明显低于阴性对照组(P<0.05)。53.6%(37/69)卵巢癌组织中有MIF蛋白表达,MIF蛋白表达与肿瘤临床分期呈显著正相关(P<0.01)。结论:MIF基因可能通过促进肿瘤细胞的增殖和侵袭能力在卵巢癌的发生发展中发挥重要作用,有可能成为分子靶向治疗卵巢癌的潜在靶点。

关键词：巨噬细胞游走抑制因子 RNA干扰卵巢肿瘤迁移侵袭

Comment on'Ascitic Fluid Analysis in the Differential Diagnosis of Ascites: Focus on Cirrhotic Ascites'

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Journal of Clinical and Translational Hepatology 2017年第2期5卷 184-184页

作者： Furqan Ahmed lin-lin Huang Harry Hua-Xiang Xia sen-lin zhu Dow Medical College Dow University of Health SciencesKarachiPakistan Department of Gastroenterology the First Affiliated HospitalSun Yat-sen UniversityGuangzhouChina Novartis Pharmaceuticals Corporation East HanoverNJUSA

First,I would like to congratulate the authors,Huang LL,Xia HH and zhu SL,on the overall great work done in preparing the review article'Ascitic Fluid Analysis in the Differential Diagnosis of Ascites:Focus on Cirrhotic Ascites'.1 However,there seems to be an error in the description of diagnosis of spontaneous bacterial peritonitis(SBP).The authors state"SBP is defined by the presence of neutrophil cells greater than or equal to 250/mL or a positive bacterial culture in the ascitic fluid without evidence of an abdominal source."The issue here is the fact that the authors make the presence of neutrophil cells greater than or equal to 250/mL for diagnoses of SBP an option(by using the conjunction term'or')and not a condition,whereas it is proven by evidence in the literature that it is a prerequisite.

关键词： diagnosis Focus preparing

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蜘蛛HMW-gDNA的电洗脱纯化提取

Zoological Research 2009年第6期30卷 627-632页

作者：陈格飞张云龙林森珠李文孟清东华大学生物科学与技术研究所上海201620

该文研究了蜘蛛大分子量基因组DNA(HMW-gDNA)的提取以及一种高效电洗脱纯化装置的构建。以蜘蛛胸部肌肉组织为原料,通过自改良CTAB法提取蜘蛛HMW-gDNA,利用透析膜和2mL离心管构建一种新的HMW-gDNA快速凝胶回收装置,并对蜘蛛HMW-gDNA进... 详细信息

该文研究了蜘蛛大分子量基因组DNA(HMW-gDNA)的提取以及一种高效电洗脱纯化装置的构建。以蜘蛛胸部肌肉组织为原料,通过自改良CTAB法提取蜘蛛HMW-gDNA,利用透析膜和2mL离心管构建一种新的HMW-gDNA快速凝胶回收装置,并对蜘蛛HMW-gDNA进行电洗脱分离回收。结果显示,改良CTAB法可高效提取蜘蛛HMW-gDNA(>48.5kb),且通过透析膜的截留作用,对普通琼脂糖凝胶中目的HMW-gDNA进行快速电洗脱分离,其回收率超过75%,OD260/OD280处于1.8～2.0之间,对HMW-gDNA完整性无影响。综合结果表明,改良CTAB法可用于蜘蛛HMW-gDNA的提取,此电洗脱纯化装置可从普通琼脂糖中高效回收HMW-gDNA,是一种低成本、简捷、高效且实用性强的凝胶回收方法。

关键词：高分子量基因组DNA 蜘蛛快速分离电洗脱