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Agricultural sciences in China 2002年第9期1卷 1054-1066页

作者： ZHANG De-li, LI Liu-Jin, XIA Geng-tian, HE Xu-yu, GAO Bu-xian, BAI Xiao-hong, HUANG Gao-sheng, LIU Shang-gao, [YEN Lung-fei] and FANG Fu-de( Department of Immunology , school of basic medical sciences, Peking University Health Science Center , Beijing 100083 , P. R . China , Beijing Institute of Preventive Medicine, Beijing 100071, P. R . China Department of Toxicology at Center for Research and Development of Animal Healthcare Products , Fourth Military medical University , Xi’an 710032 , P. R . China Department of Pathology at school of Pre-clinical Education , Section of Pathology at Xi-Jing Hospital, Institute for Cancer Research at China Cancer Research Foundation , Fourth Military medical University, Xi’an 710032 , P. R . China Key Laboratory of Preventive Veterinary Medicine of State Ministry of Agriculture , College of Animal medical sciences and State Key Laboratory of Agricultural Biotechnology, College of Biological sciences , China Agricultural University, Beijing 100094 , P.R.China State Key Laboratory of medical Molecular Biology, school of basic medical sciences at the Peking Union medical Collage /Institute of basic medical sciences at the Chinese Academy of medical sciences , Beijing 100005 , P. R . China ) Department of Immunology School of Basic Medical Sciences Peking University Health Science Center Beijing 100083 P. R. China

Under the prerequisite that the incidence of cancer or tumor in negative-control nude mice inoculated subcutaneously with primary feline or canine kidney cell cultures purified in vitro at passage 3 was 0 (0/22) and 0 (0/10), respectively. The incidence of the progressively-growing malignant tumor(MT) in positive-control nude mice inoculated subcutaneously with Hela cell cultures of KB, X, or NM20/X strain was 10/ 10, 25/25 and 5/51, respectively. The results showed that the incidence of tumor in nude mice with di-and hy-perploid YB strain of MDCK cell during 17 - 23 passages, with hyper- and hypoploid KA strain of MDCK cell during 6-8 passages, with hypoploid WB strain of MDCK cell on passage 6, with hyper-and hypoploid H strain of MDCK cell during 8-24 passages was 2/24, 6/10, 5/10 and 10/15, respectively. The chromosomal analysis results showed that the ratio of difference in the rate of modal chromosome number between high(mcs + n) and lowest (mcs)passages was not more than 5 - 15% and the structure aberrations was generally 0 -3 %. These results proved that the genetic characteristics of chromosomal number of cell lines determines their tumorigenicity, but it is species-specific. MDCK line has tumorigenicity no matter what its chromosome kary-otype is, at least it has very low tumorigenicity even when its modal chromosome number is hypoploid. It is thus evident that MDCK cell of WB or H strain can be approved as substrate for the preparation of attenuated viral vaccines, but MDCK cell of YB or KA strain can not be approved as substrate for the preparation of attenuated viral vaccines.

关键词： Cell line MDCK Master cell stock Working cell bank Karyotypes Carcinogenesis Tu-morigenicity

A quantification model for apoptosis in mouse embryos in the early stage of fetation

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Science China(Life sciences) 2009年第10期52卷 922-927页

作者： WANG PengFei1, FU JianHua1, MA WanYun1, CHEN DieYan1, Lü DanYu2 & BAI WenJia3 1 Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry, Department of Physics, Tsinghua University, Beijing 100084, China 2 Department of Anatomy & Histology and Embryology, school of basic medical sciences Peking University Health Science Center, Beijing 100083, China 3 Department of Automation, Tsinghua University, Beijing 100084, China 1. Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry Department of Physics Tsinghua University Beijing 100084 China 2. Department of Anatomy & Histology and Embryology School of Basic Medical Sciences Peking University Health Science Center Beijing 100083 China 3. Department of Automation Tsinghua University Beijing 100084 China

Apoptosis is the most important inducement and modulator for embryos in the early stage of fetation, i.e. after the 8-cell stage, mostly the morula and blastula stage, to proceed to the stage of nonlinear development. Using a two-photon laser scanning microscopy (TPLSM) system, we obtained 3-dimensional (3D) fluorescent images of preimplantation mouse embryos. A model for quantification was established. The statistical results for the spatial location of apoptosis bodies in embryos was obtained following image processing, as well as investigation of the kinetics of apoptosis. It was found that most (70%) apoptosis occurred in the trophectoderm, and the departure between the centroid and geometric center of embryos had a step transition when embryos developed into the 32-cell stage, which was consistent with the theoretical prediction that the blastocele would induce a symmetry break of the distribution of cells in embryos.

关键词： apoptosis preimplantation mouse embryo TPLSM quantification model

Effect of Carbonic Anhydrase Inhibitors on Water Channel Aquaporin 1 and Tumor Metastasis

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Effect of Carbonic Anhydrase Inhibitors on Water Channel Aqu...

Analysis of SRY Gene in 8 Cases of Sex Abnormality

2002全国青年药理学英文学术会议

作者： MA Bing and LI Xue-jun(Department of Pharmacology, school of basic medical sciences, Peking University,Beijing 100083)

AIM: The aquaporin (AQP) family of water channels plays a fundamental role in transmembrane water movements in numerous plant and animal tissues. It is increasingly clear that alterations in AQPs expression or function can be rate-limiting for water transport across certain membranes. AQPs are likely to prove central to the pathophysiology of a variety of clinical conditions and ultimately, they could be a target for therapy in diseases of altered water homeostasis. To be interesting, the tissue distribution and even the subcellular localization of AQP1 are similar to that of carbonic anhydrase, suggesting that there are

关键词： AQP1 acetazolamide topiramate tumour metastasis

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Journal of Huazhong University of Science and Technology(medical sciences) 2004年第5期24卷 503-506页

作者：王慧滕云田虹陈燕杨真荣唐艳平 Department of Genetics School of Basic Medical Sciences Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

In order to investigate the relationship between sex dysplasia and sex-determining region Y (SRY) gene, 8 patients with sexual abnormality were analyzed by cytogenetic and molecular genetic methods. Fluorescence in situ hybridization (FISH) using PY3.4, X alpha satellite, and SRY probes was performed in each case to analyze the sex chromosome translocation and gene translocation. SRY gene was amplified by polymerase chain reaction (PCR) and its mutation was detected by direct sequencing. The results showed that among 8 patients, 5 were positive for SRY and the remaining negative for SRY. In the patients positive for SRY genes, 3 presented testes and the left 2 streak ovaries. In the patients negative for SRY, only one case presented testes, while 2 ovaries. Direct sequencing demonstrated that all SRY genes were normal in the patients positive for SRY genes. FISH technique demonstrated that SRY genes translocated from Ypter to Xpter in 2 46,XX phenotypic males positive for SRY genes. It was concluded that SRY gene is strongly involved in male sex determination, while a sequence of other genes may be taken into account in sexual development.

关键词： sex determination sex-determining region gene fluorescence in situ hybridization

Expression of ATP synthase 6 in breast cancer cell line, T47D cells, induced by heregulin β1

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吉林大学学报（医学版） 2002年第2期28卷 111-114页

作者： SUN Yan bo,YANG Gui zhen (Dept.of Immunology, school of basic medical sciences,Jilin University ,Changchun 130021 China) Dept. of Immunology School of Basic Medical Sciences Jilin University Changchun 130021 China

目的 :探讨 Heregulinβ1诱导乳腺癌细胞 T47D相关基因的表达。方法 :应用差减杂交和斑点杂交技术检测乳腺癌细胞在 Heregulinβ1 ( 30 μg· L-1)作用 1 8h后 ,相关基因的表达情况。结果 :线粒体 ATP合成酶 6m RNA的表达在 1～ 6h明显... 详细信息

目的 :探讨 Heregulinβ1诱导乳腺癌细胞 T47D相关基因的表达。方法 :应用差减杂交和斑点杂交技术检测乳腺癌细胞在 Heregulinβ1 ( 30 μg· L-1)作用 1 8h后 ,相关基因的表达情况。结果 :线粒体 ATP合成酶 6m RNA的表达在 1～ 6h明显增高。结论 :乳腺癌细胞 T47D在 Here-gulinβ1刺激后 ,细胞 ATP酶

关键词：乳腺癌 T47D 基因表达线粒体 ATP

Anisodamine Inhibits Endotoxin-induced Tissue Factor Expression in Human Endothelial Cells

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Journal of Huazhong University of Science and Technology(medical sciences) 2002年第4期22卷 273-275页

作者：阮秋蓉宋建新邓仲端 DepartmentofPathology DepartmentofInfectiousDisease

By study on the effect of anisodamine on lipopolysaccharide- induced expression of tissue factor(TF) in vascular endothelial cells(EC) ,the mechanism of anisodam ine antithrom bosis,as well as in the treatm ent of bacteraemic shock was *** umbilical vein endothelial cells (HUVECs) were cultured by trypsin digestion m *** activity was measured in the lysates of HUVEC by using a single step clotting *** m RNA expression was detected by Northern *** order to evaluate a possible contribution of the nuclear factor (NF) -κB pathway on the effects observed,electrophoretic mobility shift assays(EMSA) were performed using nuclear extracts from HU VECs and NF- κB- binding *** results showed that treatment of HUVEC with L PS resulted in a significant increase in TF *** dose- dependently inhibited L PS- induced upregulation of *** effects was also confirm ed on the level of specific TF m RNA expression by Northern *** ore,EMSA showed that anisodamine com pletely abolished L PS- induced NF-κB DNA binding activity in nuclear ex- tracts from HUVECs treated with L PS together with *** results suggest thataniso- damine counteracts endothelial cell activation by inhibiting L PS- induced TF expression in these *** interference with the NF- κB pathway might- at least in part- contribute to this effect. The ability of anisodamine to counteract L PS effect on endothelial cells m ight be one underlying m echanism explaining its antithrombosis and efficacy in the treatment of bacteraemic shock.

关键词： Anisodamine endothelial cell tissue factor lipopolysaccharide shock nuclear fac- tor-κB

Phylogenetic clusters of rhizobia revealed by genome structures

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Science China(Life sciences) 2004年第3期47卷 268-278页

作者： ZHENG Junfang1, LIU Guirong1,2, ZHU Wanfu1, ZHOU Yuguang3 & LIU Shulin1,2 1. Department of Microbiology, school of basic medical sciences, Peking University, Beijing 100083, China 2. Department of Microbiology and Infectious Diseases, University of Calgary, Calgary, ABT2N 4N1, Canada 3. Institute of Microbiology, Chinese Academy of sciences, Beijing 100080, China 1. Department of Microbiology School of Basic Medical Sciences Peking University 100083 Beijing China 2. Department of Microbiology and Infectious Diseases University of Calgary ABT2N 4N1 Calgary Canada 3. Institute of Microbiology Chinese Academy of Sciences 100080 Beijing China

Rhizobia, bacteria that fix atmospheric nitrogen, are important agricultural resources. In order to establish the evolutionary relationships among rhizobia isolated from different geo-graphic regions and different plant hosts for systematic studies, we evaluated the use of physical structure of the rhizobial genomes as a phylogenetic marker to categorize these bacteria. In this work, we analyzed the features of genome structures of 64 rhizobial strains. These rhizobial strains were divided into 21 phylogenetic clusters according to the features of genome structures evaluated by the endonuclease I-CeuI. These clusters were supported by 16S rRNA compari-sons and genomic sequences of four rhizobial strains, but they are largely different from those based on the current taxonomic scheme (except 16S rRNA).

关键词： rhizobia, phylogeny, genome structure.

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LASP-1研究进展

生物化学与生物物理进展 2010年第11期37卷 1182-1187页

作者：黄浩然赵亮丁彦青南方医科大学基础医学院病理学系广州510515

LASP-1(LIM and SH3protein1)最初是由乳腺癌转移性淋巴结cDNA文库中筛选克隆得到,含有LIM和SH3两个结构域.LASP-1在乳腺癌和卵巢癌等多种恶性肿瘤中高表达,并和肿瘤发生、侵袭和转移密切相关.近年来研究还证实LASP-1是抑癌基因p53的靶... 详细信息

LASP-1(LIM and SH3protein1)最初是由乳腺癌转移性淋巴结cDNA文库中筛选克隆得到,含有LIM和SH3两个结构域.LASP-1在乳腺癌和卵巢癌等多种恶性肿瘤中高表达,并和肿瘤发生、侵袭和转移密切相关.近年来研究还证实LASP-1是抑癌基因p53的靶蛋白.目前的研究均表明LASP-1是一种新的肿瘤转移蛋白.

关键词： LASP-1 肿瘤转移抑癌基因p53 信号转导

利用比数比统计的结果推断相对危险度RR=1、RR>1或RR<1

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上海医科大学学报 1999年第6期26卷 425-427页

作者：赵耐青上海医科大学基础医学院数学教研室上海200032

目的　试图用比数比 (OR)的统计结果得出相对危险度 (RR)是否等于 1的结论 ,由此病例对照研究也能得出 2个率是否相等的统计推断。方法　推导OR与RR的关系表达式。结果　证明了总体OR与总体相对危险度RR在等于 1、大于 1和小于 1处是一... 详细信息

目的　试图用比数比 (OR)的统计结果得出相对危险度 (RR)是否等于 1的结论 ,由此病例对照研究也能得出 2个率是否相等的统计推断。方法　推导OR与RR的关系表达式。结果　证明了总体OR与总体相对危险度RR在等于 1、大于 1和小于 1处是一致的。结论　OR的统计检验结果和推断可以得出总体RR是否等于 1、大于 1或小于 1,而且P值相同 ,由此得到 2个率是否相等的统计推断。

关键词：比数比相对危险度统计推断流行病学