A quantification model for apoptosis in mouse embryos in the early stage of fetation
A quantification model for apoptosis in mouse embryos in the early stage of fetation作者机构:1. Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry Department of Physics Tsinghua University Beijing 100084 China 2. Department of Anatomy & Histology and Embryology School of Basic Medical Sciences Peking University Health Science Center Beijing 100083 China 3. Department of Automation Tsinghua University Beijing 100084 China
出 版 物:《Science China(Life Sciences)》 (中国科学(生命科学英文版))
年 卷 期:2009年第52卷第10期
页 面:922-927页
核心收录:
学科分类:0905[农学-畜牧学] 09[农学] 090501[农学-动物遗传育种与繁殖]
基 金:Supported by the National Key Basic Research Program of China (Grant No. 2007CB5119004) the National Natural Science Foundation of China (Grants No. 90919012 and 10874099) the Key Grant Project of the Chinese Ministry of Education (Grant No. 306020)
主 题:apoptosis preimplantation mouse embryo TPLSM quantification model
摘 要:Apoptosis is the most important inducement and modulator for embryos in the early stage of fetation, i.e. after the 8-cell stage, mostly the morula and blastula stage, to proceed to the stage of nonlinear development. Using a two-photon laser scanning microscopy (TPLSM) system, we obtained 3-dimensional (3D) fluorescent images of preimplantation mouse embryos. A model for quantification was established. The statistical results for the spatial location of apoptosis bodies in embryos was obtained following image processing, as well as investigation of the kinetics of apoptosis. It was found that most (70%) apoptosis occurred in the trophectoderm, and the departure between the centroid and geometric center of embryos had a step transition when embryos developed into the 32-cell stage, which was consistent with the theoretical prediction that the blastocele would induce a symmetry break of the distribution of cells in embryos.