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Asian Pacific Journal of Tropical Medicine 2020年第2期13卷 94-96页

作者： Van Lun Low Tiong Kai Tan Jamaiah Ibrahim sazaly abubakar Yvonne Ai Lian Lim Tropical Infectious Diseases Research and Education Centre(TIDREC) University of MalayaMalaysia Department of Parasitology Faculty of MedicineUniversity of MalayaMalaysia Faculty of Medicine and Defence Health National Defence University of MalaysiaMalaysia Department of Medical Microbiology Faculty of MedicineUniversity of MalayaMalaysia Centre of Excellence for Research in AIDS(CERIA) University of MalayaMalaysia

Rodent-borne leptospirosis is by far the most common bacterial zoonosis and it is an important emerging global public health concern in Southeast *** pathogens associated with rodents,especially those that live in close association with humans have been *** fill this knowledge gap,the present study was undertaken to explore other neglected disease agents that can naturally infect synanthropic rodents.

关键词： First evidence of Bartonella phoceensis and Candidatus Mycoplasma haemomuris subsp ratti in synanthropic rodents in Malaysia

Intracellular calcium ions facilitate dengue virus entry into endothelial cells and compromise endothelial barrier integrity

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Asian Pacific Journal of Tropical Medicine 2021年第11期14卷 505-516页

作者： Meng-Hooi Shu Pooi-Fong Wong Sing-Sin Sam Shih-Keng Loong Boon-Teong Teoh sazaly abubakar Tropical Infectious Diseases Research and Education Centre(TIDREC) Universiti Malaya50603 Kuala LumpurMalaysia Department of Pharmacology Faculty of MedicineUniversiti Malaya50603 Kuala LumpurMalaysia World Health Organization Collaborating Centre for Arbovirus Reference and Research(Dengue and Severe Dengue)MAA-12 Universiti Malaya50603 Kuala LumpurMalaysia

Objective:To investigate the involvement of Ca^(2+)in dengue virus(DENV)-infected human umbilical vein endothelial cells(HUVECs)and the disruption of endothelial ***:HUVECs were infected with DENV-2 in the presence of intracellular Ca^(2+)or endoplasmic reticulum Ca^(2+)*** infectivity was measured by focus-forming assay and quantitative *** Ca^(2+)was measured using Fluo-4-AM ***-cadherin and focal adhesion kinase(FAK)expressions were investigated by immunofluorescence and immunoblotting assays,***:DENV infection increased intracellular cytosolic Ca^(2+)levels and caused disassembly of the adherens junction protein,VEcadherin as evidenced by decreased VE-cadherin expression at the periphery of DENV-2 infected *** of intracellular Ca^(2+)stores,particularly those of the endoplasmic reticulum Ca^(2+),significantly decreased DENV yield in *** virus yield following the depletion of intracellular Ca^(2+)was caused by the inhibition of viral entry into HUVECs and not the inhibition of viral binding or ***-2 infection also resulted in Ca^(2+)-dependent activation of ***:Intracellular Ca^(2+)is required for the early phases of DENV infection in endothelial *** cytosolic Ca^(2+)levels in endothelial cells during DENV infection activated FAK,disrupted adherens junctions and compromised barrier ***,Ca^(2+)plays an important role in DENV infection in endothelial cells.

关键词： Endothelial cells Calcium signalling Dengue virus Endothelium permeability Intracellular calcium

Misidentification of multidrug resistant Enterococcus faecium using a commercial identification method

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Asian Pacific Journal of Tropical Medicine 2020年第10期13卷 474-476页

作者： Shih Keng Loong Nurul Asma Anati Che-Mat-Seri Nur Hidayana Mahfodz sazaly abubakar Tropical Infectious Diseases Research&Education Centre University of Malaya50603 Kuala LumpurMalaysia Department of Medical Microbiology Faculty of Medicine50603 Kuala LumpurMalaysia

Enterococcus(E.)faecium is recognized as a leading cause of nosocomial infections *** with the organism is often difficult to treat due to its inherent ability to acquire glycopeptide resistance genes and other virule... 详细信息

关键词： identification multidrug resistant

Human Enterovirus 71 DNA Vaccine Constructs Containing 5’UTR with Complete Internal Ribosome Entry Site Sequence Stimulated Improved Anti-Human Enterovirus 71 Neutralizing Immune Responses

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World Journal of Vaccines 2014年第1期4卷 33-43页

作者： Nor-Aziyah Mat-Rahim sazaly abubakar Tropical Infectious Diseases Research and Education Center (TIDREC) Department of Medical Microbiology Faculty of Medicine University Malaya Kuala Lumpur Malaysia Virology Unit Institute for Medical Research Kuala Lumpur Malaysia. Tropical Infectious Diseases Research and Education Center (TIDREC) Department of Medical Microbiology Faculty of MedicineUniversity Malaya Kuala Lumpur Malaysia

Recent improvement in the technologies for efficient delivery of DNA vaccines has renewed interest in the DNA-based vaccines. Several DNA-based vaccines against human enterovirus 71 (EV71), the causative agent for hand, foot and mouth disease (HFMD) have been developed. Here we examined the potential of improving the vaccines by inserting the EV71 5’ untranslated region (5’ UTR) containing the full length internal ribosome entry site (IRES) sequence to the EV71 VP1-based DNA vaccine constructs. Four vaccine constructs designated as 5’ UTR-VP1/EGFP, VP1/EGFP, 5’ UTR-VP1/pVAX and VP1/pVAX, were designed using the pEGFP-N1 and pVAX-1 expression vectors, respectively. Transfection of Vero cells with the vaccine constructs with the 5’-UTR (5’-UTR-VP1/EGFP and 5’ UTR-VP1/pVAX) resulted in higher percentages of cells expressing the recombinant protein in comparison to cells transfected with vectors without the 5’-UTR (67% and 57%, respectively). Higher IgG responses (29%) were obtained from mice immunized with the DNA vaccine construct with the full length 5’ UTR. The same group of mice when challenged with life EV71 produced significantly higher neutralizing antibody (NAb) titers (>5-fold). These results suggest that insertion of the EV71 5’ UTR sequence consisting of the full length IRES to the EV71 DNA vaccine constructs improved the efficacy of the constructs with enhanced elicitation of the neutralizing antibody responses.

关键词： Human Enterovirus 71 5’Untranslated Region (5’UTR) Internal Ribosome Entry Site (IRES) DNA Vaccine Neutralizing Antibodies

Isolation of Kytococcus schroeteri from the brown rat Rattus norvegicus

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Asian Pacific Journal of Tropical Medicine 2021年第4期14卷 191-192页

作者： Shih Keng Loong Nurul Asma Anati Che-Mat-Seri Kim-Kee Tan Noor-Syahida Azizan Siti Noraisah Ahmad-Nasrah Siti Nursheena Mohd-Zain sazaly abubakar Tropical Infectious Diseases Research&Education Centre High Impact Research BuildingUniversity of Malaya50603 Kuala LumpurMalaysia Department of Medical Microbiology Faculty of MedicineUniversity of Malaya50603 Kuala LumpurMalaysia Institute of Biological Sciences Faculty of ScienceUniversity of Malaya50603 Kuala LumpurMalaysia

Rodents such as Rattus rattus and Rattus norvegicus have adapted to living close to human settlements[1].This presents a public health challenge as these rodents are considered as pests that can transmit a number of zoonotic diseases to humans[1,2].A rodent surveillance programme was initiated between the years 2014 and 2015,focusing on rodents captured at wet markets where there is abundant food and considerable human *** present study was conducted in several wet markets in Kuala Terengganu,Malaysia(5.3333°N,103.1500°E).

关键词： Rattus initiated abundant

Unusual developing sites of dengue vectors and potential epidemiological implications

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Asian Pacific Journal of Tropical Biomedicine 2012年第3期2卷 228-232页

作者： Hamady Dieng Rahman GM Saifur Abu Hassan Ahmad MR Che Salmah Al Thbiani Aziz Tomomitsu Satho Fumio Miake Zairi Jaal sazaly abubakar Ronald Enrique Morales School of Biological Sciences Universiti Sains Malaysia Faculty of Pharmaceutical Sciences Fukuoka University Department of Medical Microbiology University of Malaya Faculty of Tropical Medicine Mahidol University

Objective:To identify the unusual breeding sites of two dengue vectors,*** albopiclus(***) and Aedes aegypti(***).Methods:During the second half of 2010, we performed an occasional survey in rural(Teluk Tempoyak) and urban(Gelugor) areas of Penang Island,Malaysia,to identify cryptic breeding ***:In the rural area,we found heterogeneous immature stages of *** in the water bowl of an encaged *** also observed *** eggs deposited in the flush tank of a toilet in the urban ***: It can be concluded that both breeding patterns can increase contact with hosts(humans and birds) and presumably population densities of *** and ***,thereby potentially boosting the risks for spread and transmission of arboviral diseases.

关键词： Breeding sites Aedes albopictus Bird Aedes aegypti Toilet Epidemiological implications