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Upconversion FRET quantitation:the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses

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Light(Science & Applications) 2022年第10期11卷 2321-2334页

作者： Agata Mkotulska Aleksandra Pilch-WrÓbel satu lahtinen Tero Soukka Artur Bednarkiewicz Division of Biomedical Physicochemistry Institute of Low Temperature and Structure ResearchPANul.Okolna 2Wroclaw 50-422Poland Department of Life Technologies/Biotechnology University of TurkuKiinamyllynkatu 1020520 TurkuFinland

Lanthanide-doped colloidal nanoparticles capable of photon upconversion(UC)offer long luminescence lifetimes,narrowband absorption and emission spectra,and efficient anti-Stokes *** features are highly advantageous for F?rster Resonance Energy Transfer(FRET)based *** nanoparticles(UCNPs)as donors may solve the existing problems of molecular FRET systems,such as photobleaching and limitations in quantitative analysis,but these new labels also bring new *** we have studied the impact of the core-shell compositional architecture of upconverting nanoparticle donors and the mode of photoexcitation on the performance of UC-FRET from UCNPs to Rose Bengal(RB)molecular *** have quantitatively compared luminescence rise and decay kinetics of Er3+emission using core-only NaYF4:20%Yb,2%Er and core-shell NaYF4:20%Yb@NaYF4:20%Yb,5%Er donor UCNPs under three photoexcitation schemes:(1)direct short-pulse photoexcitation of Er3+at 520 nm;indirect photoexcitation of Er3+through Yb3+sensitizer with(2)980 nm short(5-7 ns)or(3)980 nm long(4 ms)laser *** donor luminescence kinetics and steady-state emission spectra differed between the UCNP architectures and excitation *** for highly sensitive kinetic upconversion FRET-based biomolecular assays,the experimental results underline the complexity of the excitation and energy-migration mechanisms affecting the Er3+donor responses and suggest ways to optimize the photoexcitation scheme and the architecture of the UCNPs used as luminescent donors.

关键词： excitation donor decay

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Lanthanide-based bulky counterions against aggregation-caused quenching of dyes in fluorescent polymeric nanoparticles

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Aggregate 2022年第1期3卷 168-177页

作者： Caterina Severi satu lahtinen Jaana Rosenberg Andreas Reisch Tero Soukka Andrey S.Klymchenko Laboratoire de Biophotonique et Pathologies Faculte de PharmacieUMR 7021 CNRSUniversite de StrasbourgCedexIllkirchFrance Department of Biotechnology University of TurkuTurkuFinland

Dye-loaded polymeric nanoparticles(NPs)are promising bioimaging agents because of their available surface chemistry,high brightness,and tunable optical ***,high dye loadings can cause the aggregation-caused quenching(ACQ)of the encapsulated ***,we proposed to mitigate the ACQ inside polymeric NPs by insulating cationic dyes with bulky hydrophobic *** order to implement new functionalities into dye-loaded NPs,here,we extend the concept of bulky counterions to anionic lanthanide-based *** show that by employing Gd-based counterions with octadecyl rhodamine B loaded NPs at 30 wt% versus polymer,the fluorescence quantum yield can be increased to 10-fold(to 0.34).Moreover,Gd-anion provides NPs with enhanced contrast in electron microscopy.A combination of a luminescent Eu-based counterion with a far-red to near-infrared cyanine 5 dye(DiD)yields Forster resonance energy transfer NPs,where the UV-excited Eu-based counterion transfers energy to DiD,generating delayed fluorescence and large stokes shift of -340 *** studies reveal low cytotoxicity of NPs and their capacity to internalize without detectable dye leakage,in contrast to leaky NPs with small *** findings show that the aggregation behavior of cationic dyes in the polymeric NPs can be controlled by bulky lanthanide anions,which will help in developing bright luminescent multifunctional nanomaterials.

关键词： aggregation-caused quenching dye-loaded polymeric nanoparticles fluorescence microscopy

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