Upconversion FRET quantitation:the role of donor photoexcitation mode and compositional architecture on the decay and intensity based responses
作者机构：Division of Biomedical PhysicochemistryInstitute of Low Temperature and Structure ResearchPANul.Okolna 2Wroclaw 50-422Poland Department of Life Technologies/BiotechnologyUniversity of TurkuKiinamyllynkatu 1020520 TurkuFinland
出 版 物：《Light(Science & Applications)》 (光（科学与应用)（英文版）)
年 卷 期：2022年第11卷第10期
页 面：2321-2334页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 070301[理学-无机化学] 0702[理学-物理学]
基 金：This researchwas funded in part by National Science Centre Poland Grant No.2021/41/N/ST5/02753.Fo
主 题：excitation donor decay
摘 要：Lanthanide-doped colloidal nanoparticles capable of photon upconversion(UC)offer long luminescence lifetimes,narrowband absorption and emission spectra,and efficient anti-Stokes *** features are highly advantageous for F?rster Resonance Energy Transfer(FRET)based *** nanoparticles(UCNPs)as donors may solve the existing problems of molecular FRET systems,such as photobleaching and limitations in quantitative analysis,but these new labels also bring new *** we have studied the impact of the core-shell compositional architecture of upconverting nanoparticle donors and the mode of photoexcitation on the performance of UC-FRET from UCNPs to Rose Bengal(RB)molecular *** have quantitatively compared luminescence rise and decay kinetics of Er3+emission using core-only NaYF4:20%Yb,2%Er and core-shell NaYF4:20%Yb@NaYF4:20%Yb,5%Er donor UCNPs under three photoexcitation schemes:(1)direct short-pulse photoexcitation of Er3+at 520 nm;indirect photoexcitation of Er3+through Yb3+sensitizer with(2)980 nm short(5-7 ns)or(3)980 nm long(4 ms)laser *** donor luminescence kinetics and steady-state emission spectra differed between the UCNP architectures and excitation *** for highly sensitive kinetic upconversion FRET-based biomolecular assays,the experimental results underline the complexity of the excitation and energy-migration mechanisms affecting the Er3+donor responses and suggest ways to optimize the photoexcitation scheme and the architecture of the UCNPs used as luminescent donors.
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