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FDG and Amyloid PET in Cognitively Normal Individuals at Risk for Late-Onset Alzheimer’s Disease

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Advances in Molecular Imaging 2014年第2期4卷 15-26页

作者： John Murray Wai H. Tsui Yi Li Pauline McHugh schantel Williams Megan Cummings Elizabeth Pirraglia Lilja solnes Ricardo Osorio Lidia Glodzik shankar Vallabhajosula Alexander Drzezga satoshi Minoshima Mony J. de Leon Lisa Mosconi New York University school of Medicine New York USA University of Cologne Cologne Germany University of Washington Seattle USA Weill Cornell Medical College New York USA

Having a parent affected by late-onset Alzheimer’s disease (AD) is a major risk factor for cognitively normal (NL) individuals. This study explores the potential of PET with 18F-FDG and the amyloid-β (Aβ) tracer 11C-Pittsburgh Compound B (PiB) for detection of individual risk in NL adults with AD-parents. Methods: FDG- and PiB-PET was performed in 119 young to late-middle aged NL individuals including 80 NL with positive family history of AD (FH+) and 39 NL with negative family history of any dementia (FH-). The FH+ group included 50 subjects with maternal (FHm) and 30 with paternal family history (FHp). Individual FDG and PiB scans were Z scored on a voxel-wise basis relative to modality-specific reference databases using automated procedures and rated as positive or negative (+/-) for AD-typical abnormalities using predefined criteria. To determine the effect of age, the cohort was separated into younger (49 ± 9 y) and older (68 ± 5 y) groups relative to the median age (60 y). Results: Among individuals of age >60 y, as compared to controls, NL FH+ showed a higher frequency of FDG+ scans vs. FH- (53% vs. 6% p scans (27% vs. 11%;p = 0.19). This effect was observed for both FHm and FHp groups. Among individuals of age ≤60 y, NL FHm showed a higher frequency of FDG+ scans (29%) compared to FH- (5%, p = 0.04) and a trend compared to FHp (11%) (p = 0.07), while the distribution of PiB+ scans was not different between groups. In both age cohorts, FDG+ scans were more frequent than PiB+ scans among NL FH+, especially FHm (p s a significant predictor of FH+ status. Classification according to PiB status was significantly less successful. Conclusions: Automated analysis of FDG- and PiB-PET demonstrates higher rates of abnormalities in at-risk FH+ vs FH-subjects, indicating potentially ongoing early AD-pathology in this population. The frequency of metabolic abnormalities was higher than that of Aβ pathology in the younger cohort, suggesting that neuronal dysfunction may

关键词： Alzheimer’s Disease Early Detection Positron Emission Tomography Amyloid Imaging Glucose Metabolism Normal Aging

Mitogen-activated protein kinase-dependent apoptosis in norcan-tharidin-treated A375-s2 cells is proceeded by the activation of protein kinase C

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中华医学杂志(英文版) 2005年第3期 23-28页

作者： AN Wei-wei, WANG Min-wei, Tashiro shin-ichi, Onodera satoshi and Ikejima TakashiChina-Japan Research Institute of Medical and Pharmaceutical sciences, shenyang Pharmaceutical University, shenyang 110016, China Department of Pharmacology, shenyang Pharmaceutical University, shenyang 110016, China Department of Clinical and Biomedical sciences, showa Pharmaceutical University, Tokyo 19-8543, Japan

Background We have reported that norcantharidin (NCTD) induces human melanoma A375-s2 cell apoptosis and that the activation of caspase and the mitochondrial pathway are involved in the apoptotic process. This study aimed at investigating the roles of mitogen-activated protein kinase (MAPK) and protein kinase C (PKC) in A375-s2 cell apoptosis induced by NCTD.

关键词： norcantharidin · A375-s2 cells · mitogen-activated protein kinase · protein kinase C · staurosporine

在双面加热的窄缝环形流道中流动沸腾换热研究

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核动力工程 2001年第2期22卷 113-117,143页

作者：沈秀中宫崎庆次井村谕徐济鋆上海交通大学上海200030 日本大阪大学

在窄缝流道内发生沸腾换热现象时，由于沸腾产生的汽泡受窄缝流道的限制，受压变形而消除了汽泡表面张力对传热的影响。因此对此现象进行基础性理论研究具有很重要的意义。本文在常压下用蒸馏水对窄缝间隙为 0.75mm的垂直环形流道，进... 详细信息

在窄缝流道内发生沸腾换热现象时，由于沸腾产生的汽泡受窄缝流道的限制，受压变形而消除了汽泡表面张力对传热的影响。因此对此现象进行基础性理论研究具有很重要的意义。本文在常压下用蒸馏水对窄缝间隙为 0.75mm的垂直环形流道，进行了流动沸腾传热实验研究。实验段的有效加热长度为 900mm，其加热方式为内外侧双面加热，实验的流量变化范围在 1.67× 10－ 5～ 5.83× 10－ 5m3/s。通过实验得到了在不同质量流速和热流密度下双面加热的窄缝流道中内外侧沸腾换热系数随干度变化的分布和特点。研究结果表明，由于在窄缝流道中存在着大量的运动聚合受压变形汽泡，因此使内外侧沸腾换热系数都很高 (可达 105W· m－ 2· K－ 1以上 )。

关键词：双面加热流动沸腾换热传热强化窄缝环形流道

Near-infrared polarimetry of the GG Tauri A binary system

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Research in Astronomy and Astrophysics 2014年第11期14卷 1438-1446页

作者： Yoichi Itoh Yumiko Oasa Tomoyuki Kudo Nobuhiko Kusakabe Jun Hashimoto Lyu Abe Wolfgang Brandner Timothy D.Brandt Joseph C.Carson sebastian Egner Markus Feldt Carol A.Grady Olivier Guyon Yutaka Hayano Masahiko Hayashi saeko s.Hayashi Thomas Henning Klaus W.Hodapp Miki Ishii Masanori Iye Markus Janson Ryo Kandori Gillian R.Knapp Masayuki Kuzuhara Jungmi Kwon Taro Matsuo Michael W.McElwain shoken Miyama Jun-Ichi Morino Amaya Moro-Martin Tetsuo Nishimura Tae-soo Pyo Eugene serabyn Takuya suenaga Hiroshi suto Ryuji suzuki Yasuhiro H.Takahashi Naruhisa Takato Hiroshi Terada Christian Thalmann Daigo Tomono Edwin L.Turner Makoto Watanabe John Wisniewski Toru Yamada satoshi Mayama Thayne Currie Hideki Takami Tomonori Usuda Motohide Tamura Nishi-Harima Astronomical Observatory Center for AstronomyUniversity of Hyogo407-2NishigaichiSayoHyogo 679-5313Japan Faculty of Education Saitama University255 Shimo-OkuboSakuraSaitamaSaitama338-8570Japan subaru Telescope National Astronomical Observatory of Japan650 North Ahoku PlaceHiloHI 96720USA National Astronomical Observatory of Japan 2-21-1OsawaMitakaTokyo181-8588Japan H.L.Dodge Department of Physics & Astronomy University of Oklahoma440 W Brooks St.NormanOK 73019USA Laboratoire Lagrange (UMR 7293) Universit de Nice-Sophia AntipolisCNRSObservatoire de la Cote d’Azur28 avenue Valrose06108 Nice Cedex 2France Max Planck Institute for Astronomy Knigstuhl 1769117 HeidelbergGermany Department of Astrophysical science Princeton UniversityPeyton HallIvy LanePrincetonNJ 08544USA Department of Physics and Astronomy College of Charleston58 Coming St.CharlestonSC29424USA subaru Telescope National Astronomical Observatory of Japan650 North A’ohoku PlaceHiloHI 96720USA Exoplanets and stellar Astrophysics Laboratory Code 667Goddard Space Flight CenterGreenbeltMD 20771USA Eureka scientific 2452 DelmerSuite 100Oakland CA 96002USA Goddard Center for Astrobiology GreenbeltMD 20771USA Institute for Astronomy University of Hawaii640 N.Aohoku PlaceHiloHI 96720USA Department of Earth and Planetary sciences Tokyo Institute of TechnologyOokayamaMeguro-kuTokyo 152-8551Japan Department of Astronomy Kyoto UniversityKitashirakawa-Oiwake-choSakyo-kuKyotoKyoto 606-8502Japan Hiroshima University 1-3-2KagamiyamaHigashihiroshimaHiroshima 739-8511Japan Department of Astrophysics CAB-CSIC/INTA28850 Torrejón de ArdozMadridSpain Jet Propulsion Laboratory California Institute of TechnologyPasadenaCA91109USA Department of Astronomical science The Graduate University for Advanced Studies2-21-1OsawaMitakaTokyo181-8588Japan Department of Astronomy The University of Tokyo7-3-1HongoBunkyo-kuTokyo113-0033Japan Astronomical Institute "Anton Pannekoek" University

A high angular resolution near-infrared image that shows the intensity of polarization for the GG Tau A binary system was obtained with the subaru Telescope. The image shows a circumbinary disk scattering the light from the central binary. The azimuthal profile of the intensity of polarization for the circumbinary disk is roughly reproduced by a simple disk model with the Henyey-Greenstein phase function and the Rayleigh function, indicating there are small dust grains at the surface of the disk. Combined with a previous observation of the circumbinary disk, our image indicates that the gap structure in the circumbinary disk orbits counterclockwise, but material in the disk orbits clockwise. We propose that there is a shadow caused by material located between the central binary and the circumbinary disk. The separations and position angles of the stellar components of the binary in the past 20 yr are consistent with the binary orbit with a = 33.4 AU and e = 0.34.

关键词： stars individual （GG Tauri） -- stars pre-maln sequence - techniques high angular resolution

去甲斑蝥素诱导人黑色素瘤A375s2细胞凋亡

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中国医院药学杂志 2005年第6期25卷 501-504页

作者：安巍巍龚显峰王敏伟田代真一小野寺敏池岛乔沈阳药科大学中日医药研究所辽宁沈阳110016 沈阳药科大学GLP中心辽宁沈阳110016 昭和药科大学病态科学教研室

目的:研究去甲斑蝥素(NCTD)诱导人黑色素瘤A375-s2细胞凋亡的机制。方法:采用MTT法、形态学观察、DNA凝胶电泳及Westernblot检测法。结果:去甲斑蝥素可诱导A375-s2细胞发生凋亡。半胱氨酸天冬氨酸酶(caspase)-3,-9抑制剂可以部分的抑制N... 详细信息

目的:研究去甲斑蝥素(NCTD)诱导人黑色素瘤A375-s2细胞凋亡的机制。方法:采用MTT法、形态学观察、DNA凝胶电泳及Westernblot检测法。结果:去甲斑蝥素可诱导A375-s2细胞发生凋亡。半胱氨酸天冬氨酸酶(caspase)-3,-9抑制剂可以部分的抑制NCTD诱导的细胞死亡。细胞凋亡时caspase-3,8,-9酶活力升高,caspase3底物caspase-3激活的DNA酶抑制物(ICAD)蛋白表达下降,同时Bcl2/Bax、BclxL/Bax蛋白表达比率明显降低。结论:去甲斑蝥素通过激活caspase和Bcl-2家族诱导A375-s2细胞凋亡。

关键词：去甲斑螯素 A375-s2细胞凋亡caspase Bcl-2家族

The Effects of Bisphosphate on Chondrocyte Apoptosis in Adjuvant Arthritis

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The Effects of Bisphosphate on Chondrocyte Apoptosis in Adju...

水飞蓟素在紫外线照射诱导A375-s2细胞凋亡中发挥抑制作用

第三届国际骨质疏松及骨矿盐疾病学术会议

作者： CAO Yongping~1 YANG Xin~1 LIU Chao~1 WEN Licheng~1 satoshi Mori~2 Tomoyuki Akiyama~2 Tasuku Mashiba~2 1 Department of Orthopaedic surgery Peking University First Hospital Beijing 100034 China 2 Department of Orthopedic surgery Medical faculty Kagawa University Japan

＜正＞Objective:To investigate the effects of bisphosphate on chondrocyte apoptosis in rat adjuvant arthritis. Method:Eighty seven-week-old female Lewis rats were randomly allocated into 4 groups（20 rats per group）:normal control group（NV）, arthritic control group（sV）,low dose therapy group（s（?）-10）,high dose therapy group（s（?）-100）.

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中国病理生理杂志 2005年第10期21卷 1873-1878页

作者：李林昊吴立军田代真一小野寺敏内海文彰池岛乔沈阳药科大学中日医药研究所天然药物研究室辽宁沈阳110016 沈阳药科大学天然药物研究室辽宁沈阳110016 昭和药科大学病态科学教研室东京理科大学药学部遗传子制御学教研室沈阳药科大学中日医药研究所辽宁沈阳110016

目的:从天然药物中筛选出抑制紫外线照射诱导人黑色素瘤细胞A375-s2凋亡的有效单体。方法:MTT法测定细胞生长抑制率;形态学观察,DNA凝胶电泳及LDH法;用半胱天冬酶活力检测试剂盒测定半胱天冬酶活力;用免疫印记法检测Bcl-2家族成员(Bcl... 详细信息

目的:从天然药物中筛选出抑制紫外线照射诱导人黑色素瘤细胞A375-s2凋亡的有效单体。方法:MTT法测定细胞生长抑制率;形态学观察,DNA凝胶电泳及LDH法;用半胱天冬酶活力检测试剂盒测定半胱天冬酶活力;用免疫印记法检测Bcl-2家族成员(Bcl-2,Bcl-xL和Bax)的表达。结果:紫外线照射(2．4 J／cm2,5 min)能显著诱导A375-s2细胞发生凋亡,其作用呈明显时间依赖性。形态学观察可见凋亡小体的形成,琼脂糖凝胶电泳可见凋亡典型的DNA梯带;水飞蓟素具有抑制紫外线照射(2．4 J／cm2,5 min)诱导A375-s2细胞凋亡的作用,水飞蓟素作用于紫外线照射(2．4 J／cm2,5 min)的A375-s2细胞,培养12 h,使紫外线照射诱导的半胱天冬酶 -9、半胱天冬酶-3的活力降低;免疫印记法检测发现水飞蓟素作用的A375-s2细胞(紫外线照射)中Bcl-2蛋白和Bcl-xL蛋白的表达增加。结论:水飞蓟素明显抑制紫外线照射诱导的A375-s2细胞的凋亡,其抑制凋亡作用与半胱天冬酶途径和线粒体途径相关。

关键词：紫外线 A375-s2细胞水飞蓟素细胞凋亡半胱氨酸天冬氨酸蛋白酶

水飞蓟素和Fas激动性抗体CH11共同作用诱导A375-s2细胞凋亡

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中国药理学通报 2006年第10期22卷 1242-1245页

作者：杨小珂李林昊周蓓吴立军田代真一小野寺敏池岛乔沈阳药科大学中日医药研究所辽宁沈阳110016 沈阳药科大学天然药物研究室辽宁沈阳110016 昭和药科大学病态科学教研室

目的水飞蓟素和CH11共同作用诱导人黑色素瘤细胞A375-s2凋亡的分子机制。方法结晶紫法测定细胞生长抑制率;细胞凋亡的形态学观察,LDH法测定凋亡与坏死的比率;用免疫印迹法检测凋亡抑制性的去乙酰化酶sIRT1、Bc l-2家族成员(抗凋亡蛋白Bc... 详细信息

目的水飞蓟素和CH11共同作用诱导人黑色素瘤细胞A375-s2凋亡的分子机制。方法结晶紫法测定细胞生长抑制率;细胞凋亡的形态学观察,LDH法测定凋亡与坏死的比率;用免疫印迹法检测凋亡抑制性的去乙酰化酶sIRT1、Bc l-2家族成员(抗凋亡蛋白Bc l-2,Bc l-xL和促凋亡蛋白Bax)、细胞色素C和Caspase-3的表达。结果水飞蓟素和CH11共同作用能诱导A375-s2细胞发生凋亡;形态学观察可见凋亡小体的形成;免疫印迹法检测发现水飞蓟素和CH11共同作用的A375-s2细胞中Bax蛋白的表达增加,Bc l-2蛋白和Bc l-xL蛋白的表达降低,细胞色素C释放增加,pro-caspase-3表达量明显降低。结论水飞蓟素协同CH11能明显促进A375-s2细胞的凋亡。

关键词：水飞蓟素 CH11 协同作用 A375-s2细胞凋亡

Leukemogenic Fusion Gene (p190 BCR-ABL) Transduction into Hematopoietic stem/Progenitor Cells in the Common Marmoset

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Open Journal of Blood Diseases 2012年第1期2卷 1-10页

作者： Yan Dong seiichiro Kobayashi Yamin Tian Manabu Ozawa Takafumi Hiramoto Kiyoko Izawa Yuansong Bai Yasushi soda Erika sasaki Toshio Itoh Yoshiro Maru satoshi Takahashi Kaoru Uchimaru Naoki Oyaizu Arinobu Tojo Chieko Kai Kenzaburo Tani Central Institute for Experimental Animals Kawasaki Japan Department of Laboratory Medicine Research Hospital Institute of Medical Science the University of Tokyo Tokyo Japan Department of Molecular Genetics Medical Institute of Bioregulation Kyushu University Fukuoka Japan Department of Pharmacology Tokyo Woman’s Medical University Japan Division of Molecular Therapy Institute of Medical Science the University of Tokyo Tokyo Japan Laboratory Animal Research Center Institute of Medical Science the University of Tokyo Tokyo Japan.

Patients with Philadelphia chromosome (p190 BCR-ABL fusion gene)-positive acute lymphoblastic leukemia have a poor prognosis despite intensive therapeutic intervention.In this study, we attempted to develop a leukemia nonhuman primate model that mimics various human systems. Hematopoietic stem/progenitor cells in the common marmoset were transduced with a lentiviral vector containing the p190 BCR-ABL fusion gene by ex vivo transduction or in vivo direct bone marrow injection. In the latter model, BCR-ABL gene expression was maintained for more than one and a half years. One marmoset unexpectedly developed myelofibrosis-like disease. However, none of the marmosets have developed leukemia to date. In conclusion, we successfully achieved sustained p190 BCR-ABL gene expression in vivo. However, a genetic mutation in addition to p190 BCR-ABL may be required for the malignant transformation of hematopoietic stem/progenitor cells in the common marmoset during the short observation period. This novel in vivo approach will help develop a marmoset leukemia model in the future.

关键词： Leukemia Lentiviral Vector Myelofibrosis Common Marmoset