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Establishment of mouse intestinal myofibroblast cell lines

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World Journal of Gastroenterology 2013年第17期19卷 2629-2637页

作者： Hideyoshi Kawasaki Takashi Ohama Masatoshi Hori koichi sato Laboratory of Veterinary Pharmacology Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Pharmacology Graduate School of Agriculture and Life Sciences the University of Tokyo

AIM:To establish novel intestinal myofibroblast (IMF) cell lines from mouse colonic mucosa and investigate their biological characters. METHODS:Primary IMFs were isolated from mucosal tissues of mouse colon that was denuded of epithelial cells and smooth muscle layer. For immortalization, primary IMFs were transfected with simian virus 40 large T antigen (designated as LmcMF). We also isolated some primary IMFs that spontaneously became immortalized without transfection (designated as SmcMF). To check immortality and normality of these cells, we examined their proliferative ability and contact inhibition. Moreover, the expression levels of proteins characterizing IMFs [including α-smooth muscle actin (α-SMA), vimentin, desmin, and type Ⅰ collagen] and proteins associated with the immune response [such as toll-like receptor 4 (TLR-4), CD14, MD2, IκBα, and p-p38] were determined by Western blotting. The localization of several myofibroblast protein markers was also detected by immunofluorescence staining. RESULTS:The cell growth assay results show that both LmcMF and SmcMF cells proliferated logarithmically at least up to passage 20. In addition, the contact inhibition assays show that LmcMF and SmcMF stopped growing after the cells reached confluence. These data suggest that these 2 types of cells were immortalized without losing contact inhibition of growth. Moreover, both LmcMF and SmcMF, like primary IMFs, showed spindle-shaped appearance. The expression levels of key myofibroblast protein markers, including α-SMA, vimentin, and desmin, were also examined by the Western blotting and immunofluorescence analyses. Our results show that these cells were positive for α-SMA and vimentin, but not desmin, as well as that both LmcMF and SmcMF expressed type Ⅰ collagen at a lower level than primary IMFs. Finally, we investigated the expression level of lipopolysaccharide (LPS) receptor-related proteins, as well as the response of the cells to LPS treatment. We found that th

关键词： Cell line Colon Lipopolysaccharide Mouse Myofibroblast

Role of sphingosine 1-phosphate in anti-atherogenic actions of high-density lipoprotein

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World Journal of Biological Chemistry 2010年第11期1卷 327-337页

作者： koichi sato Fumikazu Okajima Laboratory of Signal Transduction Institute for Molecular and Cellular RegulationGunma University

The reverse cholesterol transport mediated by high-density lipoprotein (HDL) is an important mechanism for maintaining body cholesterol, and hence, the crucial anti-atherogenic action of the lipoprotein. Recent studies, however, have shown that HDL exerts a variety of anti-inflammatory and anti-atherogenic actions independently of cholesterol metabolism. The present review provides an overview of the roles of sphingosine 1-phosphate (S1P)/S1P receptor and apolipoprotein A-I/scavenger receptor class B type I systems in the anti-atherogenic HDL actions. In addition, the physiological significance of the existence of S1P in the HDL particles is discussed.

关键词： High-density lipoprotein Sphingosine 1-phosphate Scavenger receptor class B type I Anti-atherogenic actions

Analysis on the long term discharge of a catchment

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Journal of Environmental Sciences 2000年第3期12卷 279-285页

作者： HONG Lin 1, TAKASE Keiji 2, sato koichi 2 (1. Wuhan University of Hydraulic and Electric Engineering, Wuhan 430072, China 2. Ehime University, Japan) Wuhan University of Hydraulic and Electric Engineering Wuhan 430072 China Ehime University Japan

In this study, the characteristics of the long term discharge of a Terraced Paddy Field Catchment are studied. 2 Kind of Tank Models are proposed and used to simulate the discharge of the catchment. The characteristics of the model simulations of the discharge of the catchment are analyzed and compared with those of a forest catchment.

关键词： long term discharge model simulation terraced paddy field catchment

Rate of social anxiety disorder, its comorbidity with depression and paroxetine effects in outpatients in Japan

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Open Journal of Psychiatry 2013年第1期3卷 191-194页

作者： Yukihiko Shirayama Michio Takahashi Masatoshi Suzuki Atsushi Kimura koichi sato Department of Psychiatry Teikyo University Chiba Medical Center Ichihara Japan

The prevalence of persons with social anxiety disorder (SAD) in Japan remains unknown. This study examined 293 patients with age between 20 and 60 at first visit on the outpatient clinic of psychiatry by the section of social phobia of M.I.N.I. and DSM-IV. After that, 10 patients with both SAD out of 16 patients (trial recruited) completed 12 weeks of treatment with paroxetine. Among 63 patients with 4 points and 40 patients with 3 points on the M.I.N.I., 21 patients (33%) and 16 patients (40%) were diagnosed as SAD on DSM-IV criteria, respectively. Together, 37 patients (12.6%) were diagnosed as SAD out of the 293 outpatients. Among 37 patients with SAD, 23 patients (62%) had comorbid depression. As for 10 patients after treatment with paroxetine, 8 patients improved from the point of recovery of depression (HAM-D scores below 10), whereas only 4 patients improved from the point of recovery of social phobia (L-SAS scores below 30). Three points as well as 4 points on the M.I.N.I. is meaningful for the diagnosis of SAD. For a while, paroxetine exerted less beneficial effects on SAD rather than on depression.

关键词： Social Anxiety Disorder Depression Paroxetine Comorbidity

Analysis of Predictive Factors for Lymph Node Metastasis in Submucosal Invasive Colorectal Carcinoma

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Surgical Science 2014年第3期5卷 75-83页

作者： Kiichi Sugimoto koichi sato Hiroshi Maekawa Mutsumi Sakurada Hajime Orita Tomoaki Ito Ryo Wada Department of Pathology Juntendo University Shizuoka Hospital 1129 Nagaoka Izunokuni Shizuoka 410-2295 Japan Department of Surgery Juntendo University Shizuoka Hospital 1129 Nagaoka Izunokuni Shizuoka 410-2295 Japan

Purpose: Submucosal invasive colorectal carcinoma (SICC) exhibits lymph node metastasis in about 10% of patients. Therefore, endoscopic resection is insufficient for cases of SICC at risk of lymph node metastasis, and surgical resection accompanied with lymph node dissection is necessary. However, because additional intestinal resection is unnecessary for cases without lymph node metastasis, more rigid criteria are required in order to decrease the incidence of unnecessary further intestinal resection. We retrospectively identified predictive factors for lymph node metastasis in submucosal invasive colorectal carcinoma. Methods: One hundred and two patients who underwent intestinal resection as the first treatment or additional intestinal resection after endoscopic resection at our department between 1999 and 2012 were enrolled in the present study. Clinicopathological factors were analyzed to determine predictive factors related to lymph node metastasis. Results: The multivariate analysis revealing only depth of submucosal invasion (≤2700 μm) was found to be a significant, independent predictive factor of lymph node metastasis (P = 0.04, Odds ratio: 4.18, 95% CI: 1.06 - 16.40). Conclusion: It is considered that the refinement of the criteria in the present study will be very useful, especially in the patients for whom careful judgment is required when considering additional intestinal resection.

关键词： Submucosal Invasive Colorectal Carcinoma Lymph Node Metastasis Additional Intestinal Resection Endoscopic Resection Depth of Submucosal Invasion

Effects of hydrogen charging and deformation on tensile properties of a multi-component alloy for nuclear applications

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Tungsten 2022年第3期4卷 212-218页

作者： Te Zhu Zhi-Hong Zhong koichi sato Ya-Min Song Feng-Jiao Ye Qian-Qian Wang Ye Dong Peng Zhang Run-Sheng Yu Bao-Yi Wang Alfonso H.W.Ngan Xing-Zhong Cao Qiu Xu Department of Mechanical Engineering The University of Hong KongPokfulam RoadHong KongChina Institute of High Energy Physics Chinese Academy of SciencesBeijing100049China Institute for Integrated Radiation and Nuclear Science Kyoto UniversityOsaka5900494Japan School of Materials Science and Engineering Hefei University of TechnologyHefei230009China Graduate School of Science and Engineering Kagoshima UniversityKagoshima8900065Japan

In this study,the influence of hydrogen charging and deformation on the tensile behavior of a 60Fe-12Cr-10Mn-15Cu-3Mo multi-component alloy was investigated using electron microscopy and positron annihilation lifetime *** results show that hydrogen-induced vacancy clusters found in the electrochemically charged hydrogen specimens are responsible for crack *** ingress to the microstructure,hydrogen promotes the formation of cell-structured dislocations that are beneficial for the improvement of tensile *** addition,hydrogen embrittlement can be mitigated by dislocations that can hinder hydrogen mobility in the deformed specimens.

关键词： Hydrogen embrittlement Dislocation cell Multi-component alloys

Pathophysiological analysis and strategy for stercoral perforation of the colon

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Open Journal of Gastroenterology 2012年第2期2卷 45-50页

作者： koichi sato Hiroshi Maekawa Mutsumi Sakurada Hajime Orita Tomoaki Ito Yoshihiro Komatsu Fumiko Hirata Ryo Wada Department of Pathology Juntendo Shizuoka Hospital Juntendo University School of Medicine Department of Surgery Juntendo Shizuoka Hospital Juntendo University School of Medicine

Perpose: In order to establish the pathophysiological features and strategy for stercoral perforation of the colon, we herein analyze a series of stercoral perforation of the colon. Method: Ten patients were diagnosed with stercoral perforation. Clinical features, primary diseases, triggers, causative bacteria in ascites, postoperative complications, pathological features, severity of the disease, and effect of direct hemoperfusion with polymyxin B immobilized fiber (PMX-DHP) were investigated. Results: Nine patients had a long history of serious and chronic constipation and 7 patients had hypertension. Causative bacteria in ascites during the operation were most commonly Escherichia coli. There were a lot of severe postoperative complications such as sepsis, disseminated intravascular coagulation, and acute lung injury. With regard to the microscopic findings of the perforation site, the intestinal wall showed severe nonspecific inflammatory changes, including an increase of mono-nuclear cells in the lamina propria. There were 4 hospital deaths, so the mortality rate was 40%. APACHE- II and SOFA score were high postoperation and 24 hours after the operation. PMX-DHP was performed in 8 cases of severe conditions of stercoral perforation of the colon. Because the catecholamine index improved within 24 hours, four of 8 cases were rescued. Conclusion: Most of the patients with stercoral perforation of the colon had severe postoperative complications. The severity of the disease was extremely high, therefore, early diagnosis based on pathophy-siological features and comprehensive therapies including PMX-DHP were necessary for strategy of treating stercoral perforation of the colon.

关键词： Stercoral Perforation of the Colon APACHE-II Score SOFA Score Polymyxin B Immobilized Fiber (PMX-DHP)

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从对仇兆鳌的毁誉来看《杜诗详注》的价值

闽南师范大学学报（哲学社会科学版） 2022年第2期36卷 55-60页

作者：佐藤浩一东海大学日本东京都涩谷区

中日学界对仇兆鳌《杜诗详注》的评价往往毁誉参半。将清代以来学者对《杜诗详注》的褒贬之词进行梳理,并进一步结合其成书背景及相关文献考察这些评价允当与否。可知,诸家对《杜诗详注》价值缺陷的指摘有失公允,有必要重新对相关诋毁... 详细信息

中日学界对仇兆鳌《杜诗详注》的评价往往毁誉参半。将清代以来学者对《杜诗详注》的褒贬之词进行梳理,并进一步结合其成书背景及相关文献考察这些评价允当与否。可知,诸家对《杜诗详注》价值缺陷的指摘有失公允,有必要重新对相关诋毁与称誉重新审视:一是针对《杜诗详注》的引文中含有仇氏杜撰成分的说法,其实,同时代的注释书几乎都存在更改原文的地方,而在明清时期改字解杜风气盛行的背后《,详注》的引文本身也极有可能已经被改字了;二是针对《杜诗详注》的注释含有冗赘、画蛇添足的要素之说,特别在音注方面,之所以令读者感到冗长乏味,与作者本着诲人不倦的启蒙原则是不无关联的;三是针对《杜诗详注》中仇氏频频将其出生地域的发音视作杜甫的中古音的发音的情况,无疑属于仇氏的注杜之失,可从另一个角度来看《,杜诗详注》中存在的大量音注又为我们了解当时所在地域的发音提供宝贵的资料。

关键词：仇兆鳌《杜诗详注》诋毁称誉

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杜甫的早期诗歌——是散佚还是废弃

杜甫研究学刊 2021年第2期 20-27页

作者： [日]佐藤浩一日本东海大学

杜甫一生勤奋咏诗,现存诗歌1457首,比同时代诗人的多出许多。无论从作品的质量上还是数量上,杜甫都不愧为代表盛唐的一位诗杰。但杜甫实际上的作品数量应该更多。现存的杜诗,几乎没有他30岁之前的作品。日本和中国学界对此各有其不同的... 详细信息

杜甫一生勤奋咏诗,现存诗歌1457首,比同时代诗人的多出许多。无论从作品的质量上还是数量上,杜甫都不愧为代表盛唐的一位诗杰。但杜甫实际上的作品数量应该更多。现存的杜诗,几乎没有他30岁之前的作品。日本和中国学界对此各有其不同的解释。在日本,废弃说主流,即认为杜甫30岁时和李白相遇,领略了李白的稀世才能,决定废弃自己早期咏的诗歌,立志以一种与李白完全不同的诗风来重新咏诗。而中国多数人认为杜甫早期的作品未曾留下是自然散佚。这两种不同的见解到底哪一种正确呢?

关键词：废弃说散佚说早期作品