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High-throughput physical phenotyping of cell differentiation

Microsystems & Nanoengineering 2017年第1期3卷 316-322页

作者： Jonathan Lin Donghyuk Kim Henry T.Tse Peter Tseng Lillian Peng Manjima Dhar saravanan karumbayaram Dino Di Carlo Department of Bioengineering University of CaliforniaLos AngelesCA 90095USA CytoVale Inc. 384 Oyster Point Boulevard#7 SouthSan FranciscoCA 94080USA Department of Biomedical Engineering Tufts UniversityMedfordMA 02155USA Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research Los AngelesCA 90095USA California NanoSystems Institute Los AngelesCA 90095USA Jonsson Comprehensive Cancer Center Los AngelesCA 90095USA Department of Mechanical Engineering University of CaliforniaLos AngelesCA 90095USA

In this report,we present multiparameter deformability cytometry(m-DC),in which we explore a large set of parameters describing the physical phenotypes of pluripotent cells and their derivatives.m-DC utilizes microfluidic inertial focusing and hydrodynamic stretching of single cells in conjunction with high-speed video recording to realize high-throughput characterization of over 20 different cell motion and morphology-derived *** extracted from videos include size,deformability,deformation kinetics,and *** train support vector machines that provide evidence that these additional physical measurements improve classification of induced pluripotent stem cells,mesenchymal stem cells,neural stem cells,and their derivatives compared to size and deformability *** addition,we utilize visual interactive stochastic neighbor embedding to visually map the high-dimensional physical phenotypic spaces occupied by these stem cells and their progeny and the pathways traversed during *** report demonstrates the potential of m-DC for improving understanding of physical differences that arise as cells differentiate and identifying cell subpopulations in a label-free ***,such approaches could broaden our understanding of subtle changes in cell phenotypes and their roles in human biology.

关键词： cell mechanics deformation deformation kinetics morphology physical phenotype stem cells

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