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Wound healing activity of aqueous extract of Crotalaria verrucosa in Wistar albino rats

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Asian Pacific Journal of Tropical Medicine 2010年第10期3卷 783-787页

作者： Meena Kumari Eesha BR Mohanbabu Amberkar sarath babu Rajshekar Neelesh Kumar Department of Pharmacology Kasturba Medical CollegeManipal University

Objective:To evaluate the wound healing effect of aqueous extract of Crotalaria verrucosa(C. verrucosa) in ***:Three wound models including incision,excision and dead space wounds were used in this *** parameters studied were breaking strength in incision models,granulation tissue dry weight,breaking strength and hydroxyproline content in dead space wounds,percentage of wound contraction and period of epithelialization in excision wound ***:Two doses of the extract with and without dexamethasone showed significant increases in mean hydroxyproline,total protein content and dry weight of granulation tissue but it was higher with dose 800 mg/kg comparing with the *** dexamethasone treated group showed a significant(P<0.001) reduction in the wound breaking strength when compared to control group in incision type of wound *** of *** with dexamethasone significantly(P<0.001) increased the breaking strength compared to the dexamethasone treated only *** excision wound model,the percentage of the wound contraction was significantly(P<0.01) increased by two doses of test extract on all the days except the lower dose which exhibited only on 12th,16th days of drug treatment and it also reversed the dexamethasone suppressed wound *** significantly(P<0.001) reduced the time required for epithelialization and reversed the epithelialization delaying effect of dexamethasone (P<0.001).Conclusions:*** was found to possess significant wound healing property. This was evident by decrease in the period of epithelialization,increase in the rate of wound contraction,skin breaking strength,and granulation tissue dry weight *** C. verrucosa could be a good wound healing agent.

关键词： Crotalaria verrucosa Incision wound Excision wound Deadspace wound Wound breaking strength

Effects of dissolved oxygen on intestinal bacterial community and immunity of Atlantic salmon Salmo salar

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Journal of Oceanology and Limnology 2023年第1期41卷 364-375页

作者： Shihong XU Yanfeng WANG Caixia GAO sarath babu V Jun LI Qinghua LIU Zhizhong XIAO Yingxuan XU Chunyan ZHAO Li LIN Liang CHI Department of Aquatic Animal Medicine College of FisheriesHuazhong Agricultural UniversityWuhan 430070China College of Veterinary Medicine Qingdao Agricultural UniversityQingdao 266109China Laboratory Animal and Comparative Medicine Team State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural Sciences(CAAS)Harbin 150069China CAS Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Guangdong Provincial Water Environment and Aquatic Products Security Engineering Technology Research Center Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl BreedingZhongkai University of Agriculture and EngineeringGuangzhou 510225China University College London London WC1E 6BTUnited Kingdom School of Marine Science and Engineering Qingdao Agricultural UniversityQingdao 266109China

Dissolved oxygen(DO)is one of most important factors which affect wide range physiologic features of including immune responses and intestinal bacterial ***,the underlying mechanisms remain *** address this question,the intestinal bacterial community compositions and the immune features of Atlantic salmon(Salmo salar)grown in recirculating aquaculture systems(RAS)were *** were reared under different DO saturation levels,e.g.,200% saturation named high group(H),100%saturation named control group(CK),and 60%saturation named lower group(L).Large variations in the operational taxonomic units(OTUs)frequency distribution for the intestinal bacterial community of Atlantic salmon were *** intestinal bacterial community of all groups was dominated mainly by three phyla,e.g.,***,and ***,Acinetobacter baumannii,an opportunistic pathogen of salmon was increased significantly in L *** further monitored the immunity features of fish under different DO *** results show that leucocyte number,cortisol level,the expressions of interleukin-1β(IL-1β),Toll-like receptor 4(TLR4),and nucleotide-binding oligomerization domain like protein 2(NOD2)were higher at significant levels in the L group than those in the other two ***4 and NOD2 are usually related with the bacterial infections;therefore,it is reasonable to believe that the stronger immune responses observed in the L group might be related with the higher abundance of *** in the inte stine of Atlantic ***,these findings demonstrated that low DO level may induce stronger immunity responses in Atlantic salmon.

关键词： Atlantic salmon dissolved oxygen(DO) immune responses microbiota intestine

Inhibition of Fish Nodavirus by Gymnemagenol Extracted from Gymnema sylvestre

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Journal of Ocean University of China 2011年第4期10卷 402-408页

作者： V.Gopiesh Khanna K.Kannabiran V.sarath babu A.S.Sahul Hameed School of Biosciences and Technology VIT University Aquaculture Biotechnology Division Department of ZoologyC.Abdul Hakeem College

Viral nervous necrosis(VNN)has emerged to become a major problem in the culture of larval and juvenile marine fish *** phytochemicals isolated from commonly available medicinal plants are often screened for their efficacy in controlling fish viral *** of newer viral strains and resistance to existing antiviral drugs are problems currently as-sociated with treatment of VNN,which necessitates looking for alternate sources for effective antiviral *** aim of the present study was to screen antiviral potential of gymnemagenol(C30H50O4)previously extracted from leaves of Gymnema *** fish nodavirus,grouper nervous necrosis virus(GNNV)in infected Sahul Indian Grouper Eye(SIGE)cell lines were used to study the antiviral activity of gymnemagenol under in vitro *** susceptibility of the virus to gymnemagenol was confirmed by measuring the viral titre(TCID50 mL?1)in virus-infected SIGE cells every 24 *** at 20 μg mL?1 inhibited the prolifera-tion of GNNV to 53% at the end of the 6th d by inhibiting the proliferation of GNNV-infected SIGE *** viable SIGE cells were reduced to 47% as determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)*** viral titre(TCID50 mL?1)was also reduced to log 2.8 at the end of the 7th d in gymnemagenol-treated SIGE cells after inoculated with GNNV when compared to untreated control SIGE cell viral titre(log 4.1).Based on our results it can be concluded that gymnemagenol could be used as an antiviral agent against GNNV infection.

关键词： Gymnema sylvestre gymnemagenol grouper nervous necrosis virus Sahul Indian Grouper Eye cell line

罗氏沼虾几丁质酶3B基因的克隆及其在蜕皮周期中的表达

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水产学报 2019年第4期43卷 751-762页

作者：叶成凯卢志杰 sarath babu V 张晓君刘晓丹赵丽娟潘淦林蠡仲恺农业工程学院动物科技学院广东省水环境与水产品安全工程技术研究中心广州市水产病害与水禽养殖重点实验室华南师范大学生命科学学院广东省水产健康安全养殖重点实验室扬州大学动物科技学院

罗氏沼虾的生长发育及繁殖都与蜕皮紧密相关。几丁质酶是甲壳动物在蜕皮过程中最重要的酶之一。本研究对罗氏沼虾蜕皮周期外观特征和腹肢刚毛进行了观察描述,克隆并分析了罗氏沼虾的几丁质酶Ⅲ基因(MrChi3B),制备了几丁质酶Ⅲ蛋白的多... 详细信息

罗氏沼虾的生长发育及繁殖都与蜕皮紧密相关。几丁质酶是甲壳动物在蜕皮过程中最重要的酶之一。本研究对罗氏沼虾蜕皮周期外观特征和腹肢刚毛进行了观察描述,克隆并分析了罗氏沼虾的几丁质酶Ⅲ基因(MrChi3B),制备了几丁质酶Ⅲ蛋白的多克隆抗体,研究了其在蜕皮周期中的表达。罗氏沼虾几丁质酶基因cDNA的开放阅读框为1 143 bp,编码380个氨基酸,大小为41.91 ku。通过氨基酸序列比对发现,MrChi3B与日本沼虾几丁质酶基因(Chi3B)的同源性最高,为94%。MrChi3B含有一个糖苷键水解酶家族18的催化域。实时定量PCR (qRT-PCR)和蛋白印迹法(WB)检测结果显示,MrChi3B在罗氏沼虾多个组织中均有表达,但是不同组织中的表达有差异。在蜕皮期之后,其在胃、表皮和肌肉中的表达量显著上升。在胃中其表达量在蜕皮间期达到最高;在表皮和肌肉中其表达量在蜕皮后期达到最高;肠中的表达量在蜕皮前期和蜕皮期有所上升;眼柄期的表达量在所有蜕皮期都偏低。本研究结果为进一步研究几丁质酶的功能提供参考依据。

关键词：罗氏沼虾几丁质酶基因克隆蜕皮周期表达

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罗氏沼虾谷氨酰胺合成酶基因的克隆与表达

水产学报 2019年第3期43卷 549-562页

作者：卢志杰叶成凯 sarath babu V 张晓君刘晓丹赵丽娟潘淦林蠡华南师范大学生命科学学院广东省水产健康安全养殖重点实验室仲恺农业工程学院动物科技学院广东省水环境与水产品安全工程技术研究中心广州市水产病害与水禽养殖重点实验室扬州大学动物科技学院

谷氨酰胺合成酶(glutamine synthetase, GS)是一种广泛分布在动植物体内的酶,并参与细胞多种代谢调控。在甲壳动物中,GS在能量代谢和渗透压调节过程中起着重要作用。实验克隆了罗氏沼虾GS基因。GS基因cDNA全长1 965 bp,开放读码框(ORF)... 详细信息

谷氨酰胺合成酶(glutamine synthetase, GS)是一种广泛分布在动植物体内的酶,并参与细胞多种代谢调控。在甲壳动物中,GS在能量代谢和渗透压调节过程中起着重要作用。实验克隆了罗氏沼虾GS基因。GS基因cDNA全长1 965 bp,开放读码框(ORF)为1 086 bp,编码361个氨基酸(aa),分子量大小为40.75 ku,等电点为5.81。进化树分析发现,罗氏沼虾GS基因与凡纳滨对虾、斑节对虾和中国明对虾GS聚为一支,氨基酸相似性达到95%。氨基酸多序列比对分析结果显示,罗氏沼虾GS属于无脊椎动物分支的GSⅡ群,有5个保守区域。实验对罗氏沼虾GS蛋白进行表达并制备了多克隆抗体。采用荧光定量PCR技术(qRT-PCR)和免疫印迹(Western blot)对罗氏沼虾蜕壳前后的不同组织GS表达进行检测。qRT-PCR结果显示,GS基因在所检测的8个组织中均有表达;蜕壳前,其相对表达量顺序为:肝胰脏>肌肉>胃>肠>鳃>心脏>脑>血淋巴。蜕壳后和蜕壳前GS基因在组织中的差异表达比较结果显示,除了在肝胰脏表达下调外,其他7个组织都表达升高,其相对表达量的差异顺序为:脑>鳃>胃>肠>肌肉>心脏>血淋巴。Westernblot结果显示,蜕壳后GS蛋白在鳃和肌肉组织表达量上调,与其mRNA表达一致。此外,罗氏沼虾蜕壳后肝胰脏GS酶的活性和谷氨酰胺的含量下调,而其在鳃、肌肉和血淋巴中上调,结果与其基因表达一致。研究表明,GS基因在不同组织中表达的差异可能和罗氏沼虾的能量代谢、渗透压调节有关。

关键词：罗氏沼虾谷氨酰胺合成酶组织表达蜕壳

Hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatocellular damage in Wistar albino rats

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Asian Pacific Journal of Tropical Medicine 2011年第6期4卷 466-469页

作者： Eesha BR Mohanbabu Amberkar V Meena Kumari K sarath babu Vijay M Lalit M Rajput R Department of Pharmacology Kasturba Medical CollegeManipal UniversityKarnatakaIndia

Objective:To evaluate the hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatic damage in ***:The plant material was shade dried, powdered and extracted with *** 52 and silymarin were used as standard drugs and 2%gum acacia as a control(vehicle).Alteration in the levels of biochemical markers of hepatic damage like AST,ALT,ALP and lipid peroxides were tested,and phytochemical tests were also ***:Paracetamol(2 g/kg) increased the serum levels of alanine aminotransfer (ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) and the lipid peroxides. Treatment of Liv 52,silymarin and ethanolic extract of Terminalia paniculata(200 mg/kg) altered levels of biochemical marker and showed significant hepatoprotective *** extract revealed the presence of phenolic compound and *** findings suggested that ethanolic bark extract of Terminalia paniculata possessed hepatoprotective activity in a dose dependent ***:Terminalia paniculata possesses hepatoprotective *** could be an effective and promising preventive agent against PCT induced hepatotoxicity.

关键词： Terminalia paniculata Hepatoprotection Paracetamol Liv 52 Silymarin

抑制肠道气呼吸大鳞副泥鳅主要呼吸器官的组织病理

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水产学报 2020年第5期44卷 845-857页

作者：王玟秀秦真东 sarath babu V 周萌梁日深莫金凤吴灶和张轩赵丽娟林蠡仲恺农业工程学院动物科技学院广东省水环境与水产品安全工程技术研究中心广州市水产病害与水禽养殖重点实验室广州市华轩水产有限公司

为了阐明肠道气呼吸对泥鳅的生理作用,本研究通过抑制大鳞副泥鳅肠道气呼吸,探究其主要呼吸器官的组织病理变化。结果显示,被抑制肠道气呼吸的大鳞副泥鳅通常在1周左右死亡。当被抑制肠道气呼吸的大鳞副泥鳅出现垂死时,采集对照组和实... 详细信息

为了阐明肠道气呼吸对泥鳅的生理作用,本研究通过抑制大鳞副泥鳅肠道气呼吸,探究其主要呼吸器官的组织病理变化。结果显示,被抑制肠道气呼吸的大鳞副泥鳅通常在1周左右死亡。当被抑制肠道气呼吸的大鳞副泥鳅出现垂死时,采集对照组和实验组的鳃、皮肤、前肠、中肠、后肠以及直肠进行苏木素—伊红(H.E)染色、阿利新蓝—高碘酸雪夫氏(AB-PAS)染色组织切片观察和扫描电镜观察,主要的病理变化:①H.E染色结果显示实验组大鳞副泥鳅鳃丝末端充血,背部皮肤表皮层的毛细血管收缩并减少,且真皮层细胞呈畸形,前肠黏膜褶膨大,后肠浆膜层有血红细胞渗出,后肠、直肠结缔组织显著增厚;②AB-PAS染色结果显示,实验组大鳞副泥鳅鳃、背部皮肤、后肠、直肠组织中嗜酸性空泡细胞均增多,前肠和中肠固有层酸性黏蛋白含量增多,黏膜下层中性黏蛋白含量减少;③扫描电镜结果显示,实验组大鳞副泥鳅鳃丝鳃小片表面皱缩,表皮受损脱落,背部皮肤表面分泌孔增加,中肠内腔表面突起增多,后肠和直肠絮状颗粒增多。研究表明,抑制大鳞副泥鳅肠道气呼吸会引发其主要呼吸器官上皮组织出现黏液细胞增多、血红细胞溢出等病理变化,甚至导致机体死亡,由此可知,肠道气呼吸行为是大鳞副泥鳅的必要生理活动。本研究将为大鳞副泥鳅的健康养殖及幼苗培育提供新的参考。

关键词：大鳞副泥鳅气呼吸抑制呼吸器官组织病理

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弓形革囊星虫体腔液细胞的结构与分类

仲恺农业工程学院学报 2020年第4期33卷 18-21页

作者：李凤麟 sarath babu 秦真东林蠡仲恺农业工程学院动物科技学院广东广州510225 广东省水环境与水产品安全工程技术研究中心广东广州510225 广州市水产病害与水禽养殖重点实验室广东广州510225

弓形革囊星虫(Phascolosoma arcuatum)是一种栖息在潮间带沙泥中的名优经济动物,目前已经对弓形革囊星虫开展了小规模人工养殖,但是关于其基础生理学特性知之甚少.本研究通过瑞氏姬姆萨染色、扫描电镜和透射电镜开展弓形革囊星虫体腔液... 详细信息

弓形革囊星虫(Phascolosoma arcuatum)是一种栖息在潮间带沙泥中的名优经济动物,目前已经对弓形革囊星虫开展了小规模人工养殖,但是关于其基础生理学特性知之甚少.本研究通过瑞氏姬姆萨染色、扫描电镜和透射电镜开展弓形革囊星虫体腔液细胞的形态及结构研究.结果表明,弓形革囊星虫的体腔液细胞主要存在七大类红细胞、盘状细胞、嗜碱性粒细胞、血栓细胞、淋巴样细胞、网状细胞和嗜酸性粒细胞.其中,红细胞数量在体腔液细胞中所占比例最大;盘状细胞长直径最大为39~42μm;嗜碱性粒细胞近椭圆形,碱性颗粒经姬姆萨染色后为蓝色;血栓细胞常多个聚集;淋巴样细胞细胞核较大几乎充满整个细胞;网状细胞内多空泡;嗜酸性粒细胞颗粒经姬姆萨染色后为紫红色.

关键词：弓形革囊星虫体腔液细胞结构分类

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乌鳢感染鰤诺卡氏菌的转录组反应(英文)

仲恺农业工程学院学报 2018年第1期31卷 1-10页

作者：陈健安霍洁颖 sarath babu V 林蠡华中农业大学水产学院湖北武汉430070 仲恺农业工程学院动物科技学院广东广州510225

利用转录组技术对鰤诺卡氏菌(Nocardia seriolae)感染的乌鳢(Channa argus)进行分析与比较,以揭示可能的免疫机制,尤其在Toll样受体和Nod样受体的免疫反应.从乌鳢的头肾提取总RNA,采用Illumina Hi Seq TM2500进行测序和de novo转录组分... 详细信息

利用转录组技术对鰤诺卡氏菌(Nocardia seriolae)感染的乌鳢(Channa argus)进行分析与比较,以揭示可能的免疫机制,尤其在Toll样受体和Nod样受体的免疫反应.从乌鳢的头肾提取总RNA,采用Illumina Hi Seq TM2500进行测序和de novo转录组分析.乌鳢感染鰤诺卡氏菌后,对照组和试验组的clean reads分别是33 556 284(93.79%)和34 202 766(93.52%).利用Trinity软件对unigenes进行注释,从133 999条unigenes里,一共106319条unigenes得到注释,占79.34%;NR(NCBI non-redundant protein database)注释54 886(40.97%)条unigenes;39 795(29.69%)的Swiss-Prot注释;5 885(4.39%)条unigenes被KEGG(Kyoto encyclopedia of genes and genomes)注释到335条通路;5 753(4.29%)被注释到GO(Gene ontology)里.3 912条unigenes表达差异,其中1552条表达上调(39.67%)和2 360条表达下调(60.33%).Toll样受体和Nod样受体的免疫信号通路分别有28和13条差别表达基因发现有调制,12和3个上调,11和6个下调,5和4个unigenes没有显著的变化.经过转录组测序分析显示,乌鳢感染鰤诺卡氏菌后的转录复杂程度高,导致感染个体重诱导表达的基因产品的验证发现,尤其在非特异性免疫系统方面.

关键词：乌鳢(Channa argus) 鰤诺卡氏菌(Nocardia seriolae) 转录组 Toll样受体 Nod样受体组织病理学